Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome View Full Text


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Article Info

DATE

2012-04-26

AUTHORS

Eamonn P Culligan, Roy D Sleator, Julian R Marchesi, Colin Hill

ABSTRACT

Metagenomics is a powerful tool that allows for the culture-independent analysis of complex microbial communities. One of the most complex and dense microbial ecosystems known is that of the human distal colon, with cell densities reaching up to 1012 per gram of faeces. With the majority of species as yet uncultured, there are an enormous number of novel genes awaiting discovery. In the current study, we conducted a functional screen of a metagenomic library of the human gut microbiota for potential salt-tolerant clones. Using transposon mutagenesis, three genes were identified from a single clone exhibiting high levels of identity to a species from the genus Collinsella (closest relative being Collinsella aerofaciens) (COLAER_01955, COLAER_01957 and COLAER_01981), a high G+C, Gram-positive member of the Actinobacteria commonly found in the human gut. The encoded proteins exhibit a strong similarity to GalE, MurB and MazG. Furthermore, pyrosequencing and bioinformatic analysis of two additional fosmid clones revealed the presence of an additional galE and mazG gene, with the highest level of genetic identity to Akkermansia muciniphila and Eggerthella sp. YY7918, respectively. Cloning and heterologous expression of the genes in the osmosensitive strain, Escherichia coli MKH13, resulted in increased salt tolerance of the transformed cells. It is hoped that the identification of atypical salt tolerance genes will help to further elucidate novel salt tolerance mechanisms, and will assist our increased understanding how resident bacteria cope with the osmolarity of the gastrointestinal tract. More... »

PAGES

1916-1925

References to SciGraph publications

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  • 2006-11-26. Transposon-aided capture (TRACA) of plasmids resident in the human gut mobile metagenome in NATURE METHODS
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  • 2009-11-23. Probiotics and gastrointestinal disease: successes, problems and future prospects in GUT PATHOGENS
  • 1996-04. Stress response in pathogenic bacteria in JOURNAL OF BIOSCIENCES
  • 2004-06-25. Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice in MOLECULAR GENETICS AND GENOMICS
  • 2010-03. A human gut microbial gene catalogue established by metagenomic sequencing in NATURE
  • 2010-02-03. An overview of in silico protein function prediction in ARCHIVES OF MICROBIOLOGY
  • 1998-09. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments in ARCHIVES OF MICROBIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ismej.2012.38

    DOI

    http://dx.doi.org/10.1038/ismej.2012.38

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1036916589

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22534607


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