Comparative metaproteomics reveals ocean-scale shifts in microbial nutrient utilization and energy transduction View Full Text


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Article Info

DATE

2010-02-18

AUTHORS

Robert M Morris, Brook L Nunn, Christian Frazar, David R Goodlett, Ying S Ting, Gabrielle Rocap

ABSTRACT

Bacteria and Archaea play critical roles in marine energy fluxes and nutrient cycles by incorporating and redistributing dissolved organic matter and inorganic nutrients in the oceans. How these microorganisms do this work at the level of the expressed protein is known only from a few studies of targeted lineages. We used comparative membrane metaproteomics to identify functional responses of communities to different nutrient concentrations on an oceanic scale. Comparative analyses of microbial membrane fractions revealed shifts in nutrient utilization and energy transduction along an environmental gradient in South Atlantic surface waters, from a low-nutrient gyre to a highly productive coastal upwelling region. The dominant membrane proteins identified (19%) were TonB-dependent transporters (TBDTs), which are known to utilize a proton motive force to transport nutrients across the outer membrane of Gram-negative bacteria. The ocean-wide importance of TonB-dependent nutrient acquisition in marine bacteria was unsuspected. Diverse light-harvesting rhodopsins were detected in membrane proteomes from every sample. Proteomic evidence of both TBDTs and rhodopsins in the same lineages suggest that phototrophic bacterioplankton have the potential to use energy from light to fuel transport activities. We also identified viral proteins in every sample and archaeal ammonia monooxygenase proteins in the upwelling region, suggesting that Archaea are important nitrifiers in nutrient-rich surface waters. More... »

PAGES

673-685

References to SciGraph publications

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  • 2006-02. Proteorhodopsin lateral gene transfer between marine planktonic Bacteria and Archaea in NATURE
  • 2005-09. Isolation of an autotrophic ammonia-oxidizing marine archaeon in NATURE
  • 2007-03-07. Strain-resolved community proteomics reveals recombining genomes of acidophilic bacteria in NATURE
  • 2002-12. SAR11 clade dominates ocean surface bacterioplankton communities in NATURE
  • 2005-11. Proteorhodopsin in the ubiquitous marine bacterium SAR11 in NATURE
  • 2002-08. Cultivation of the ubiquitous SAR11 marine bacterioplankton clade in NATURE
  • 2004-02-01. Community structure and metabolism through reconstruction of microbial genomes from the environment in NATURE
  • 2001-01. Archaeal dominance in the mesopelagic zone of the Pacific Ocean in NATURE
  • 2008-05-13. Proteorhodopsins: an array of physiological roles? in NATURE REVIEWS MICROBIOLOGY
  • 1994-11-01. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ismej.2010.4

    DOI

    http://dx.doi.org/10.1038/ismej.2010.4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1000797192

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20164862


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