Contribution of crenarchaeal autotrophic ammonia oxidizers to the dark primary production in Tyrrhenian deep waters (Central Mediterranean Sea) View Full Text


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Article Info

DATE

2011-01-06

AUTHORS

Michail M Yakimov, Violetta La Cono, Francesco Smedile, Thomas H DeLuca, Silvia Juárez, Sergio Ciordia, Marisol Fernández, Juan Pablo Albar, Manuel Ferrer, Peter N Golyshin, Laura Giuliano

ABSTRACT

Mesophilic Crenarchaeota have recently been thought to be significant contributors to nitrogen (N) and carbon (C) cycling. In this study, we examined the vertical distribution of ammonia-oxidizing Crenarchaeota at offshore site in Southern Tyrrhenian Sea. The median value of the crenachaeal cell to amoA gene ratio was close to one suggesting that virtually all deep-sea Crenarchaeota possess the capacity to oxidize ammonia. Crenarchaea-specific genes, nirK and ureC, for nitrite reductase and urease were identified and their affiliation demonstrated the presence of ‘deep-sea’ clades distinct from ‘shallow’ representatives. Measured deep-sea dark CO2 fixation estimates were comparable to the median value of photosynthetic biomass production calculated for this area of Tyrrhenian Sea, pointing to the significance of this process in the C cycle of aphotic marine ecosystems. To elucidate the pivotal organisms in this process, we targeted known marine crenarchaeal autotrophy-related genes, coding for acetyl-CoA carboxylase (accA) and 4-hydroxybutyryl-CoA dehydratase (4-hbd). As in case of nirK and ureC, these genes are grouped with deep-sea sequences being distantly related to those retrieved from the epipelagic zone. To pair the molecular data with specific functional attributes we performed [14C]HCO3 incorporation experiments followed by analyses of radiolabeled proteins using shotgun proteomics approach. More than 100 oligopeptides were attributed to 40 marine crenarchaeal-specific proteins that are involved in 10 different metabolic processes, including autotrophy. Obtained results provided a clear proof of chemolithoautotrophic physiology of bathypelagic crenarchaeota and indicated that this numerically predominant group of microorganisms facilitate a hitherto unrecognized sink for inorganic C of a global importance. More... »

PAGES

945-961

References to SciGraph publications

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  • 2008-01-17. Molecular and biogeochemical evidence for ammonia oxidation by marine Crenarchaeota in the Gulf of California in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2010-08-14. Molecular signatures for the Crenarchaeota and the Thaumarchaeota in ANTONIE VAN LEEUWENHOEK
  • 2007-06-01. Role of nitrite reductase in the ammonia-oxidizing pathway of Nitrosomonas europaea in ARCHIVES OF MICROBIOLOGY
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  • 2001-01. Archaeal dominance in the mesopelagic zone of the Pacific Ocean in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ismej.2010.197

    DOI

    http://dx.doi.org/10.1038/ismej.2010.197

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1007955479

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21209665


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