Increased adipogenic conversion of muscle satellite cells in obese Zucker rats View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-03-16

AUTHORS

A Scarda, C Franzin, G Milan, M Sanna, C Dal Prà, C Pagano, L Boldrin, M Piccoli, E Trevellin, M Granzotto, P Gamba, G Federspil, P De Coppi, R Vettor

ABSTRACT

Aims/hypothesis:Visceral and intermuscular adipose tissue (IMAT) depots account for most obesity-related metabolic and cardiovascular complications. Muscle satellite cells (SCs) are mesenchymal stem cells giving rise to myotubes and also to adipocytes, suggesting their possible contribution to IMAT origin and expansion. We investigated the myogenic differentiation of SCs and the adipogenic potential of both preadipocytes and SCs from genetically obese Zucker rats (fa/fa), focusing on the role of Wnt signaling in these differentiation processes.Methods:SCs were isolated by single-fiber technique from flexor digitorum brevis muscle and preadipocytes were extracted from subcutaneous adipose tissue (AT). Morphological features and gene expression profile were evaluated during in vitro myogenesis and adipogenesis. Wingless-type MMTV integration site family member 10b (Wnt10b) expression was quantified by quantitative PCR in skeletal muscle and AT.Results:We did not observe any difference in the proliferation rate and in the myogenic differentiation of SCs from obese and lean rats. However, a decreased insulin-induced glucose uptake was present in myotubes originating from fa/fa rats. Under adipogenic conditions, preadipocytes and SCs of obese animals displayed an enhanced adipogenesis. Wnt10b expression was reduced in obese rats in both muscle and AT.Conclusions/interpretation:Our data suggest that the increase in different fat depots including IMAT and the reduced muscle insulin sensitivity, the major phenotypical alteration of obese Zucker rats, could be ascribed to an intrinsic defect, either genetically determined or acquired, still present in both muscle and fat precursors. The involvement of Wnt10b as a regulator of both adipogenesis and muscle-to-fat conversion is suggested. More... »

PAGES

1319-1327

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URI

http://scigraph.springernature.com/pub.10.1038/ijo.2010.47

DOI

http://dx.doi.org/10.1038/ijo.2010.47

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003904355

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20231840


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73 mesenchymal stem cells
74 morphological features
75 muscle
76 muscle insulin sensitivity
77 muscle satellite cells
78 myogenesis
79 myogenic differentiation
80 myotubes
81 obese Zucker rats
82 obese animals
83 obese rats
84 origin
85 phenotypical alterations
86 possible contribution
87 potential
88 preadipocytes
89 precursors
90 process
91 profile
92 proliferation rate
93 quantitative PCR
94 rate
95 rats
96 regulator
97 rise
98 role
99 role of Wnt
100 satellite cells
101 sensitivity
102 single-fibre technique
103 skeletal muscle
104 stem cells
105 subcutaneous adipose tissue
106 technique
107 tissue
108 tissue depots
109 uptake
110 vitro myogenesis
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