Productive in vitro infection of human umbilical vein endothelial cells and three colon carcinoma cell lines with HIV-1 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1995-04

AUTHORS

David A Cooper, Douglas D Richman, Eva Vasak, Jacques Corbeil, Louise A Evans, Paul D Edward, Paul W Mcqueen, Ronald Penny

ABSTRACT

The objective of this study was to assess the ability of HIV-1 to establish an in vitro infection of primary human umbilical vein endothelial cells (HUVEC). The HUVEC and colon carcinoma cell lines were inoculated with different isolates of HIV-1 (HIV-1SF2, HIV-1Mck and HIV-1LAI) and productive viral infection was assessed by both the detection of p24 core antigen in the culture supernatants and the presence of specific spliced HIV mRNA. The infection which was detected in the inoculated HUVEC and all the colon carcinoma cell lines could not be blocked using an antibody targeted against the CD4 receptor. Furthermore, the HIV-inoculated HUVEC secreted elevated levels of IL-6 and this increase was found to be proportional to the size of the viral inoculum. No changes in the production of IL-1 beta, TNF-alpha, IFN-alpha and IFN-gamma were detected following HIV infection. The colon carcinoma cells, however, did not secrete increased levels of these cytokines following HIV-1 inoculation. These results confirm that non-CD4 expressing cells, such as endothelial cells and certain colon epithelial cells, serve as targets and reservoirs for HIV. Moreover, the production of IL-6 by HIV-infected endothelial cells may be a contributing factor to the aberrant immunoregulation associated with HIV infection in vivo. More... »

PAGES

140

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/icb.1995.22

DOI

http://dx.doi.org/10.1038/icb.1995.22

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043049977

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7797233


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD4 Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colonic Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endothelium, Vascular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV Core Protein p24", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Umbilical Veins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "UNSW Australia", 
          "id": "https://www.grid.ac/institutes/grid.1005.4", 
          "name": [
            "National Centre in HIV Epidemiology and Clinical Research, University of NSW, Sydney, New South Wales, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cooper", 
        "givenName": "David A", 
        "id": "sg:person.010403665332.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010403665332.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Diego", 
          "id": "https://www.grid.ac/institutes/grid.266100.3", 
          "name": [
            "Departments of Pathology and Medicine, University of California San Diego, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Richman", 
        "givenName": "Douglas D", 
        "id": "sg:person.0772641340.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772641340.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UNSW Australia", 
          "id": "https://www.grid.ac/institutes/grid.1005.4", 
          "name": [
            "Centre for Immunology, St Vincent's Hospital, University of NSW, Sydney, New South Wales, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vasak", 
        "givenName": "Eva", 
        "id": "sg:person.01074013552.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074013552.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Diego", 
          "id": "https://www.grid.ac/institutes/grid.266100.3", 
          "name": [
            "Departments of Pathology and Medicine, University of California San Diego, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Corbeil", 
        "givenName": "Jacques", 
        "id": "sg:person.01325733351.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325733351.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UNSW Australia", 
          "id": "https://www.grid.ac/institutes/grid.1005.4", 
          "name": [
            "Centre for Immunology, St Vincent's Hospital, University of NSW, Sydney, New South Wales, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Evans", 
        "givenName": "Louise A", 
        "id": "sg:person.01221770006.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221770006.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UNSW Australia", 
          "id": "https://www.grid.ac/institutes/grid.1005.4", 
          "name": [
            "Centre for Immunology, St Vincent's Hospital, University of NSW, Sydney, New South Wales, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Edward", 
        "givenName": "Paul D", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UNSW Australia", 
          "id": "https://www.grid.ac/institutes/grid.1005.4", 
          "name": [
            "Centre for Immunology, St Vincent's Hospital, University of NSW, Sydney, New South Wales, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mcqueen", 
        "givenName": "Paul W", 
        "id": "sg:person.01270103206.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270103206.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "UNSW Australia", 
          "id": "https://www.grid.ac/institutes/grid.1005.4", 
          "name": [
            "Centre for Immunology, St Vincent's Hospital, University of NSW, Sydney, New South Wales, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Penny", 
        "givenName": "Ronald", 
        "id": "sg:person.01010767342.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010767342.64"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1097/00002030-199103000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001717773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002030-199103000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001717773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.11.4068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003855000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.10.3526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008080102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0098-2997(91)90022-e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008724078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0098-2997(91)90022-e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008724078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/331368a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009863498", 
          "https://doi.org/10.1038/331368a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0169-328x(91)90052-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010085311"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0169-328x(91)90052-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010085311"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(87)90021-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013321498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1989.tb02388.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013363663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1989.tb02388.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013363663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002030-199206000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024850058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002030-199206000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024850058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.1830210814", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029346623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.5.1582", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030069426"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/337368a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030810289", 
          "https://doi.org/10.1038/337368a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0016-5085(93)91064-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030932807"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.1830180818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034512474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002030-199402000-00008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039715233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002030-199402000-00008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039715233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(70)90634-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042434066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02919741", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042756789", 
          "https://doi.org/10.1007/bf02919741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(90)90252-m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043224975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(88)90348-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044671296"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(88)90348-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044671296"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-68-4-1177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045530369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0923-2516(90)90017-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047335777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0923-2516(90)90017-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047335777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.11.4287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047928780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/aid.1992.8.1567", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059223404"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/infdis/160.3.551", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059701656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-73-8-1899", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060358482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2836951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062564692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076012883", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077245426", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077314286", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077329809", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077365946", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078618997", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078755886", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079056023", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079356766", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079868704", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082762585", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082798260", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1995-04", 
    "datePublishedReg": "1995-04-01", 
    "description": "The objective of this study was to assess the ability of HIV-1 to establish an in vitro infection of primary human umbilical vein endothelial cells (HUVEC). The HUVEC and colon carcinoma cell lines were inoculated with different isolates of HIV-1 (HIV-1SF2, HIV-1Mck and HIV-1LAI) and productive viral infection was assessed by both the detection of p24 core antigen in the culture supernatants and the presence of specific spliced HIV mRNA. The infection which was detected in the inoculated HUVEC and all the colon carcinoma cell lines could not be blocked using an antibody targeted against the CD4 receptor. Furthermore, the HIV-inoculated HUVEC secreted elevated levels of IL-6 and this increase was found to be proportional to the size of the viral inoculum. No changes in the production of IL-1 beta, TNF-alpha, IFN-alpha and IFN-gamma were detected following HIV infection. The colon carcinoma cells, however, did not secrete increased levels of these cytokines following HIV-1 inoculation. These results confirm that non-CD4 expressing cells, such as endothelial cells and certain colon epithelial cells, serve as targets and reservoirs for HIV. Moreover, the production of IL-6 by HIV-infected endothelial cells may be a contributing factor to the aberrant immunoregulation associated with HIV infection in vivo.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/icb.1995.22", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1019561", 
        "issn": [
          "0818-9641", 
          "1440-1711"
        ], 
        "name": "Immunology and Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "73"
      }
    ], 
    "name": "Productive in vitro infection of human umbilical vein endothelial cells and three colon carcinoma cell lines with HIV-1", 
    "pagination": "140", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "cd0fba50a08460c7efc4650549ca7f923671676ffee378401f23d47163750d7f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7797233"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8706300"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/icb.1995.22"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1043049977"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/icb.1995.22", 
      "https://app.dimensions.ai/details/publication/pub.1043049977"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000475.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/icb199522"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/icb.1995.22'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/icb.1995.22'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/icb.1995.22'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/icb.1995.22'


 

This table displays all metadata directly associated to this object as RDF triples.

278 TRIPLES      21 PREDICATES      80 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/icb.1995.22 schema:about N01c388355e0d44d980225defd45de4c9
2 N07f99cf6302f42a0b2519c5965212a87
3 N21d72261f280473fa5f859ba73e97a59
4 N31ed37fb9c394c4f8bd7bc9082eb4748
5 N56cd459e6ef543e495a4183981293db9
6 N5dd98fb95333449bb6fdb30e9d39d8ec
7 N7f6e209251e942eaaea2b7c52a8c6b5b
8 N8ff6cf77ec4f48f6853bdb14c53533f7
9 N9d1a7e3481254307aa5959c07224a2c2
10 Na5f5b4a55215462485a8648ed71f0893
11 Nd2b0773ebcbe439abc84b87f2f349978
12 Nf988234f4343409b83cf6eaf92a53195
13 Nfcb1ef3e828f4873a26570d13d338a09
14 anzsrc-for:11
15 anzsrc-for:1107
16 schema:author Nb67f6e64d9c04e30bff983dda02c85fd
17 schema:citation sg:pub.10.1007/bf02919741
18 sg:pub.10.1038/331368a0
19 sg:pub.10.1038/337368a0
20 https://app.dimensions.ai/details/publication/pub.1076012883
21 https://app.dimensions.ai/details/publication/pub.1077245426
22 https://app.dimensions.ai/details/publication/pub.1077314286
23 https://app.dimensions.ai/details/publication/pub.1077329809
24 https://app.dimensions.ai/details/publication/pub.1077365946
25 https://app.dimensions.ai/details/publication/pub.1078618997
26 https://app.dimensions.ai/details/publication/pub.1078755886
27 https://app.dimensions.ai/details/publication/pub.1079056023
28 https://app.dimensions.ai/details/publication/pub.1079356766
29 https://app.dimensions.ai/details/publication/pub.1079868704
30 https://app.dimensions.ai/details/publication/pub.1082762585
31 https://app.dimensions.ai/details/publication/pub.1082798260
32 https://doi.org/10.1002/eji.1830180818
33 https://doi.org/10.1002/eji.1830210814
34 https://doi.org/10.1016/0003-2697(87)90021-2
35 https://doi.org/10.1016/0016-5085(93)91064-o
36 https://doi.org/10.1016/0042-6822(90)90252-m
37 https://doi.org/10.1016/0098-2997(91)90022-e
38 https://doi.org/10.1016/0169-328x(91)90052-y
39 https://doi.org/10.1016/0923-2516(90)90017-d
40 https://doi.org/10.1016/s0140-6736(70)90634-3
41 https://doi.org/10.1016/s0140-6736(88)90348-0
42 https://doi.org/10.1073/pnas.84.10.3526
43 https://doi.org/10.1073/pnas.86.11.4287
44 https://doi.org/10.1073/pnas.87.11.4068
45 https://doi.org/10.1073/pnas.89.5.1582
46 https://doi.org/10.1089/aid.1992.8.1567
47 https://doi.org/10.1093/infdis/160.3.551
48 https://doi.org/10.1097/00002030-199103000-00005
49 https://doi.org/10.1097/00002030-199206000-00005
50 https://doi.org/10.1097/00002030-199402000-00008
51 https://doi.org/10.1099/0022-1317-68-4-1177
52 https://doi.org/10.1099/0022-1317-73-8-1899
53 https://doi.org/10.1111/j.1574-6968.1989.tb02388.x
54 https://doi.org/10.1126/science.2836951
55 schema:datePublished 1995-04
56 schema:datePublishedReg 1995-04-01
57 schema:description The objective of this study was to assess the ability of HIV-1 to establish an in vitro infection of primary human umbilical vein endothelial cells (HUVEC). The HUVEC and colon carcinoma cell lines were inoculated with different isolates of HIV-1 (HIV-1SF2, HIV-1Mck and HIV-1LAI) and productive viral infection was assessed by both the detection of p24 core antigen in the culture supernatants and the presence of specific spliced HIV mRNA. The infection which was detected in the inoculated HUVEC and all the colon carcinoma cell lines could not be blocked using an antibody targeted against the CD4 receptor. Furthermore, the HIV-inoculated HUVEC secreted elevated levels of IL-6 and this increase was found to be proportional to the size of the viral inoculum. No changes in the production of IL-1 beta, TNF-alpha, IFN-alpha and IFN-gamma were detected following HIV infection. The colon carcinoma cells, however, did not secrete increased levels of these cytokines following HIV-1 inoculation. These results confirm that non-CD4 expressing cells, such as endothelial cells and certain colon epithelial cells, serve as targets and reservoirs for HIV. Moreover, the production of IL-6 by HIV-infected endothelial cells may be a contributing factor to the aberrant immunoregulation associated with HIV infection in vivo.
58 schema:genre research_article
59 schema:inLanguage en
60 schema:isAccessibleForFree true
61 schema:isPartOf N3755654090f542abbf5577270e5c23a3
62 Nbd83584b0a3841e68dece026c9017a19
63 sg:journal.1019561
64 schema:name Productive in vitro infection of human umbilical vein endothelial cells and three colon carcinoma cell lines with HIV-1
65 schema:pagination 140
66 schema:productId N2d65c07c903845c088d6e0e8032d347b
67 N413a3bb4bf384f0a91c1f18ad0a52c92
68 N7a39290cac1d4f3e9e4a4f37ad2f1641
69 Nb68bc3b44a6940b6b7040466d1f620c3
70 Nd2f45a5965ad4669a0b6478216f63301
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043049977
72 https://doi.org/10.1038/icb.1995.22
73 schema:sdDatePublished 2019-04-10T14:00
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher Nafec172065a04a72bbd0c0b46e5a7ad0
76 schema:url https://www.nature.com/articles/icb199522
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N01c388355e0d44d980225defd45de4c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name CD4 Antigens
82 rdf:type schema:DefinedTerm
83 N079421d2cd2f490397598a6c3e4f889f rdf:first sg:person.01074013552.61
84 rdf:rest N2657e8bce10840c6aba15d4d1ed2d279
85 N07f99cf6302f42a0b2519c5965212a87 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Humans
87 rdf:type schema:DefinedTerm
88 N18be1b365217412a8e745eb5528d486f rdf:first sg:person.0772641340.05
89 rdf:rest N079421d2cd2f490397598a6c3e4f889f
90 N21d72261f280473fa5f859ba73e97a59 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name HIV Core Protein p24
92 rdf:type schema:DefinedTerm
93 N2657e8bce10840c6aba15d4d1ed2d279 rdf:first sg:person.01325733351.34
94 rdf:rest Nb7a898a25c3f4125af1f002e9dcf3e79
95 N2d65c07c903845c088d6e0e8032d347b schema:name doi
96 schema:value 10.1038/icb.1995.22
97 rdf:type schema:PropertyValue
98 N31ed37fb9c394c4f8bd7bc9082eb4748 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name HIV Infections
100 rdf:type schema:DefinedTerm
101 N3755654090f542abbf5577270e5c23a3 schema:volumeNumber 73
102 rdf:type schema:PublicationVolume
103 N413a3bb4bf384f0a91c1f18ad0a52c92 schema:name nlm_unique_id
104 schema:value 8706300
105 rdf:type schema:PropertyValue
106 N4f307b72fc394da3a455d1d788f047e9 rdf:first sg:person.01270103206.45
107 rdf:rest Nfbe144b85a7b48de8f0729d6c517c7df
108 N56cd459e6ef543e495a4183981293db9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Cells, Cultured
110 rdf:type schema:DefinedTerm
111 N5dd98fb95333449bb6fdb30e9d39d8ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Colonic Neoplasms
113 rdf:type schema:DefinedTerm
114 N75c25211899a4ccc80826ba8a1bf27b0 schema:affiliation https://www.grid.ac/institutes/grid.1005.4
115 schema:familyName Edward
116 schema:givenName Paul D
117 rdf:type schema:Person
118 N7a39290cac1d4f3e9e4a4f37ad2f1641 schema:name readcube_id
119 schema:value cd0fba50a08460c7efc4650549ca7f923671676ffee378401f23d47163750d7f
120 rdf:type schema:PropertyValue
121 N7f6e209251e942eaaea2b7c52a8c6b5b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Base Sequence
123 rdf:type schema:DefinedTerm
124 N8299ad0b014e44dfb2a89b69803b1afe rdf:first N75c25211899a4ccc80826ba8a1bf27b0
125 rdf:rest N4f307b72fc394da3a455d1d788f047e9
126 N8ff6cf77ec4f48f6853bdb14c53533f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Umbilical Veins
128 rdf:type schema:DefinedTerm
129 N9d1a7e3481254307aa5959c07224a2c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name HIV-1
131 rdf:type schema:DefinedTerm
132 Na5f5b4a55215462485a8648ed71f0893 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Molecular Sequence Data
134 rdf:type schema:DefinedTerm
135 Nafec172065a04a72bbd0c0b46e5a7ad0 schema:name Springer Nature - SN SciGraph project
136 rdf:type schema:Organization
137 Nb67f6e64d9c04e30bff983dda02c85fd rdf:first sg:person.010403665332.80
138 rdf:rest N18be1b365217412a8e745eb5528d486f
139 Nb68bc3b44a6940b6b7040466d1f620c3 schema:name dimensions_id
140 schema:value pub.1043049977
141 rdf:type schema:PropertyValue
142 Nb7a898a25c3f4125af1f002e9dcf3e79 rdf:first sg:person.01221770006.83
143 rdf:rest N8299ad0b014e44dfb2a89b69803b1afe
144 Nbd83584b0a3841e68dece026c9017a19 schema:issueNumber 2
145 rdf:type schema:PublicationIssue
146 Nd2b0773ebcbe439abc84b87f2f349978 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name RNA, Viral
148 rdf:type schema:DefinedTerm
149 Nd2f45a5965ad4669a0b6478216f63301 schema:name pubmed_id
150 schema:value 7797233
151 rdf:type schema:PropertyValue
152 Nf988234f4343409b83cf6eaf92a53195 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Endothelium, Vascular
154 rdf:type schema:DefinedTerm
155 Nfbe144b85a7b48de8f0729d6c517c7df rdf:first sg:person.01010767342.64
156 rdf:rest rdf:nil
157 Nfcb1ef3e828f4873a26570d13d338a09 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Tumor Cells, Cultured
159 rdf:type schema:DefinedTerm
160 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
161 schema:name Medical and Health Sciences
162 rdf:type schema:DefinedTerm
163 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
164 schema:name Immunology
165 rdf:type schema:DefinedTerm
166 sg:journal.1019561 schema:issn 0818-9641
167 1440-1711
168 schema:name Immunology and Cell Biology
169 rdf:type schema:Periodical
170 sg:person.01010767342.64 schema:affiliation https://www.grid.ac/institutes/grid.1005.4
171 schema:familyName Penny
172 schema:givenName Ronald
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010767342.64
174 rdf:type schema:Person
175 sg:person.010403665332.80 schema:affiliation https://www.grid.ac/institutes/grid.1005.4
176 schema:familyName Cooper
177 schema:givenName David A
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010403665332.80
179 rdf:type schema:Person
180 sg:person.01074013552.61 schema:affiliation https://www.grid.ac/institutes/grid.1005.4
181 schema:familyName Vasak
182 schema:givenName Eva
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074013552.61
184 rdf:type schema:Person
185 sg:person.01221770006.83 schema:affiliation https://www.grid.ac/institutes/grid.1005.4
186 schema:familyName Evans
187 schema:givenName Louise A
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221770006.83
189 rdf:type schema:Person
190 sg:person.01270103206.45 schema:affiliation https://www.grid.ac/institutes/grid.1005.4
191 schema:familyName Mcqueen
192 schema:givenName Paul W
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270103206.45
194 rdf:type schema:Person
195 sg:person.01325733351.34 schema:affiliation https://www.grid.ac/institutes/grid.266100.3
196 schema:familyName Corbeil
197 schema:givenName Jacques
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325733351.34
199 rdf:type schema:Person
200 sg:person.0772641340.05 schema:affiliation https://www.grid.ac/institutes/grid.266100.3
201 schema:familyName Richman
202 schema:givenName Douglas D
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772641340.05
204 rdf:type schema:Person
205 sg:pub.10.1007/bf02919741 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042756789
206 https://doi.org/10.1007/bf02919741
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/331368a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009863498
209 https://doi.org/10.1038/331368a0
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/337368a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030810289
212 https://doi.org/10.1038/337368a0
213 rdf:type schema:CreativeWork
214 https://app.dimensions.ai/details/publication/pub.1076012883 schema:CreativeWork
215 https://app.dimensions.ai/details/publication/pub.1077245426 schema:CreativeWork
216 https://app.dimensions.ai/details/publication/pub.1077314286 schema:CreativeWork
217 https://app.dimensions.ai/details/publication/pub.1077329809 schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1077365946 schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1078618997 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1078755886 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1079056023 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1079356766 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1079868704 schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1082762585 schema:CreativeWork
225 https://app.dimensions.ai/details/publication/pub.1082798260 schema:CreativeWork
226 https://doi.org/10.1002/eji.1830180818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034512474
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1002/eji.1830210814 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029346623
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/0003-2697(87)90021-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013321498
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/0016-5085(93)91064-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1030932807
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/0042-6822(90)90252-m schema:sameAs https://app.dimensions.ai/details/publication/pub.1043224975
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/0098-2997(91)90022-e schema:sameAs https://app.dimensions.ai/details/publication/pub.1008724078
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/0169-328x(91)90052-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1010085311
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/0923-2516(90)90017-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1047335777
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/s0140-6736(70)90634-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042434066
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/s0140-6736(88)90348-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044671296
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1073/pnas.84.10.3526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008080102
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1073/pnas.86.11.4287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047928780
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1073/pnas.87.11.4068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003855000
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1073/pnas.89.5.1582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030069426
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1089/aid.1992.8.1567 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059223404
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1093/infdis/160.3.551 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059701656
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1097/00002030-199103000-00005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001717773
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1097/00002030-199206000-00005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024850058
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1097/00002030-199402000-00008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039715233
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1099/0022-1317-68-4-1177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045530369
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1099/0022-1317-73-8-1899 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060358482
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1111/j.1574-6968.1989.tb02388.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013363663
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1126/science.2836951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062564692
271 rdf:type schema:CreativeWork
272 https://www.grid.ac/institutes/grid.1005.4 schema:alternateName UNSW Australia
273 schema:name Centre for Immunology, St Vincent's Hospital, University of NSW, Sydney, New South Wales, Australia
274 National Centre in HIV Epidemiology and Clinical Research, University of NSW, Sydney, New South Wales, Australia
275 rdf:type schema:Organization
276 https://www.grid.ac/institutes/grid.266100.3 schema:alternateName University of California, San Diego
277 schema:name Departments of Pathology and Medicine, University of California San Diego, California, USA
278 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...