Isolation and characterization of glomerular macrophages in experimental glomerulonephritis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1988-04

AUTHORS

PG Tipping, SR Holdsworth

ABSTRACT

Adherent cells emigrating from glomeruli of rabbits developing anti-glomerular basement membrane antibody induced glomerulonephritis were isolated and characterized as macrophages. Glomeruli were isolated using a sterile graded sieving technique and cultured in plastic tissue culture flasks. After varying culture times, emigrating adherent cells were harvested by 'cold shock' or trypsin-versene. These cells had the morphological and functional characteristics of macrophages. They were largely mononuclear, esterase-positive, phagocytic cells, which exhibited surface Fc receptors. A mean of 4.8 +/- 2.1 X 10(4) macrophages could be isolated from 2 X 10(4) glomeruli after 1 h in tissue culture. Greater numbers of macrophages could be isolated with further time in culture. After 72 h however, intrinsic glomerular cell contamination occurred. The majority of the cells were viable by fluorescein diacetate hydrolysis, and Trypan Blue exclusion. Further functional studies of these cells may provide some new insights into the cellular basis of macrophage-induced glomerular injury in experimental glomerulonephritis. More... »

PAGES

icb198818

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/icb.1988.18

DOI

http://dx.doi.org/10.1038/icb.1988.18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004648765

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3181996


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