On the presence of DNA polymerase α in human lymphocyte nuclei and chromosomes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1996-08

AUTHORS

M G Ennas, H Suzuki, M Menegazzi, A Carcereri, F Hanaoka, F Gremo, M Nieddu, R Mezzanotte

ABSTRACT

Experiments were carried out to correlate the cytological localization of DNA polymerase α with the presence of its specific mRNA in human lymphocytes studied at different times after phytohaemagglutinin stimulation. Our data indicated that in resting cells it is not possible to detect DNA polymerase α protein or mRNA by Northern hybridization. By contrast, in stimulated cells the detection of mRNA specific for DNA polymerase α synthesis is possible after 16 h phytohaemagglutin stimulation, whereas immunolocalization is possible after only 4 h stimulation. Observation of cytological preparations from cells stimulated for times long enough to obtain mitoses surprisingly showed an intense immunoreaction in mitotic chromosomes treated with monoclonal antibodies to DNA polymerase α. More... »

PAGES

186-191

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/hdy.1996.123

DOI

http://dx.doi.org/10.1038/hdy.1996.123

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003611943

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8760400


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199 grid-institutes:grid.7597.c schema:alternateName Institute of Physical and Chemical Research (Riken), Wako, Saitama, Japan
200 schema:name Institute of Physical and Chemical Research (Riken), Wako, Saitama, Japan
201 rdf:type schema:Organization
202 grid-institutes:grid.7763.5 schema:alternateName Dipartimento di Citomorfologia, Università di Cagliari, Italy
203 Istituto di Biologie Generale, Università di Cagliari, Italy
204 schema:name Dipartimento di Citomorfologia, Università di Cagliari, Italy
205 Istituto di Biologie Generale, Università di Cagliari, Italy
206 rdf:type schema:Organization
 




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