Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-01

AUTHORS

D Simão, C Pinto, P Fernandes, C J Peddie, S Piersanti, L M Collinson, S Salinas, I Saggio, G Schiavo, E J Kremer, C Brito, P M Alves

ABSTRACT

Gene therapy is a promising approach with enormous potential for treatment of neurodegenerative disorders. Viral vectors derived from canine adenovirus type 2 (CAV-2) present attractive features for gene delivery strategies in the human brain, by preferentially transducing neurons, are capable of efficient axonal transport to afferent brain structures, have a 30-kb cloning capacity and have low innate and induced immunogenicity in preclinical tests. For clinical translation, in-depth preclinical evaluation of efficacy and safety in a human setting is primordial. Stem cell-derived human neural cells have a great potential as complementary tools by bridging the gap between animal models, which often diverge considerably from human phenotype, and clinical trials. Herein, we explore helper-dependent CAV-2 (hd-CAV-2) efficacy and safety for gene delivery in a human stem cell-derived 3D neural in vitro model. Assessment of hd-CAV-2 vector efficacy was performed at different multiplicities of infection, by evaluating transgene expression and impact on cell viability, ultrastructural cellular organization and neuronal gene expression. Under optimized conditions, hd-CAV-2 transduction led to stable long-term transgene expression with minimal toxicity. hd-CAV-2 preferentially transduced neurons, whereas human adenovirus type 5 (HAdV5) showed increased tropism toward glial cells. This work demonstrates, in a physiologically relevant 3D model, that hd-CAV-2 vectors are efficient tools for gene delivery to human neurons, with stable long-term transgene expression and minimal cytotoxicity. More... »

PAGES

86

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/gt.2015.75

DOI

http://dx.doi.org/10.1038/gt.2015.75

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026275828

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26181626


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenoviruses, Canine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenoviruses, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Axonal Transport", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Central Nervous System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neural Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neuroglia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Tropism", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal", 
            "Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sim\u00e3o", 
        "givenName": "D", 
        "id": "sg:person.01304011123.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304011123.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal", 
            "Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pinto", 
        "givenName": "C", 
        "id": "sg:person.01107043614.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107043614.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal", 
            "Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fernandes", 
        "givenName": "P", 
        "id": "sg:person.0616636744.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616636744.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "The Francis Crick Institute, Lincoln's Inn Fields Laboratory, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peddie", 
        "givenName": "C J", 
        "id": "sg:person.0742214210.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742214210.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapienza University of Rome", 
          "id": "https://www.grid.ac/institutes/grid.7841.a", 
          "name": [
            "Dipartimento di Biologia e Biotecnologie \u2018Charles Darwin\u2019, Universit\u00e0 di Roma La Sapienza, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Piersanti", 
        "givenName": "S", 
        "id": "sg:person.01270140430.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270140430.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "The Francis Crick Institute, Lincoln's Inn Fields Laboratory, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Collinson", 
        "givenName": "L M", 
        "id": "sg:person.0677742110.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677742110.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Montpellier", 
          "id": "https://www.grid.ac/institutes/grid.121334.6", 
          "name": [
            "Institut de G\u00e9n\u00e9tique Mol\u00e9culaire de Montpellier, Montpellier, France", 
            "Universit\u00e9 Montpellier, Montpellier, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Salinas", 
        "givenName": "S", 
        "id": "sg:person.01022143342.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022143342.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapienza University of Rome", 
          "id": "https://www.grid.ac/institutes/grid.7841.a", 
          "name": [
            "Dipartimento di Biologia e Biotecnologie \u2018Charles Darwin\u2019, Universit\u00e0 di Roma La Sapienza, Rome, Italy", 
            "Istituto Pasteur Fondazione Cenci Bolognetti, Universit\u00e0 di Roma La Sapienza, Rome, Italy", 
            "Istituto di Biologia e Patologia Molecolari del CNR, Universit\u00e0 di Roma La Sapienza, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saggio", 
        "givenName": "I", 
        "id": "sg:person.01043324530.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043324530.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University College London", 
          "id": "https://www.grid.ac/institutes/grid.83440.3b", 
          "name": [
            "Sobell Department of Motor Neuroscience and Movement Disorders, Institute of Neurology, University College London, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schiavo", 
        "givenName": "G", 
        "id": "sg:person.01255571250.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255571250.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Montpellier", 
          "id": "https://www.grid.ac/institutes/grid.121334.6", 
          "name": [
            "Institut de G\u00e9n\u00e9tique Mol\u00e9culaire de Montpellier, Montpellier, France", 
            "Universit\u00e9 Montpellier, Montpellier, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kremer", 
        "givenName": "E J", 
        "id": "sg:person.0651116711.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0651116711.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal", 
            "Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brito", 
        "givenName": "C", 
        "id": "sg:person.0747041465.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747041465.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidade Nova de Lisboa", 
          "id": "https://www.grid.ac/institutes/grid.10772.33", 
          "name": [
            "iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal", 
            "Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alves", 
        "givenName": "P M", 
        "id": "sg:person.014102654140.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014102654140.78"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pone.0060342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000868130"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1000442", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000944773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/gt.2012.52", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003225579", 
          "https://doi.org/10.1038/gt.2012.52"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2009.02.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003314348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/mb:34:2:247", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005939199", 
          "https://doi.org/10.1385/mb:34:2:247"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0023212", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006205975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/btpr.64", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006820929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0290(19961105)52:3<429::aid-bit9>3.0.co;2-m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008260782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011750309", 
          "https://doi.org/10.1038/sj.gt.3302779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011750309", 
          "https://doi.org/10.1038/sj.gt.3302779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm2236", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015693164", 
          "https://doi.org/10.1038/nrm2236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.1.505-512.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017312190"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.03-0438fje", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017721166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pneurobio.2008.05.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018762161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.01-0321fje", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018968665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0069808", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019848936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4155/tde.10.95", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020490619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/abab:133:1:9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022155349", 
          "https://doi.org/10.1385/abab:133:1:9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022297273", 
          "https://doi.org/10.1038/sj.gt.3302538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022297273", 
          "https://doi.org/10.1038/sj.gt.3302538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/exnr.2001.7706", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023213170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4155/tde.10.50", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023556570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.80.3.1487-1496.2006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025110102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymthe.2005.10.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025243513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.03576-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025872939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2165/11533740-000000000-00000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026351862", 
          "https://doi.org/10.2165/11533740-000000000-00000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2165/11533740-000000000-00000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026351862", 
          "https://doi.org/10.2165/11533740-000000000-00000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026706357", 
          "https://doi.org/10.1038/nmeth.2019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/biot.201400765", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027018540"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m605316200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029568633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.nbd.2011.09.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032486602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.22.10639-10649.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033541280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037922659", 
          "https://doi.org/10.1038/nm1113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037922659", 
          "https://doi.org/10.1038/nm1113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037922659", 
          "https://doi.org/10.1038/nm1113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/v2092134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040570415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mthe.2001.0263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043354276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2012.03.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044890446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2012.03.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044890446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/gt.2014.92", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046055806", 
          "https://doi.org/10.1038/gt.2014.92"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m113.518365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046652305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2009.08.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047597521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053182885", 
          "https://doi.org/10.1038/nature14600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/104303400750001417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059202084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/ten.tea.2014.0079", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059316569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1226625", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062466980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/1874467210801010013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069234160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2741/e607", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070918084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078138494", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-01", 
    "datePublishedReg": "2016-01-01", 
    "description": "Gene therapy is a promising approach with enormous potential for treatment of neurodegenerative disorders. Viral vectors derived from canine adenovirus type 2 (CAV-2) present attractive features for gene delivery strategies in the human brain, by preferentially transducing neurons, are capable of efficient axonal transport to afferent brain structures, have a 30-kb cloning capacity and have low innate and induced immunogenicity in preclinical tests. For clinical translation, in-depth preclinical evaluation of efficacy and safety in a human setting is primordial. Stem cell-derived human neural cells have a great potential as complementary tools by bridging the gap between animal models, which often diverge considerably from human phenotype, and clinical trials. Herein, we explore helper-dependent CAV-2 (hd-CAV-2) efficacy and safety for gene delivery in a human stem cell-derived 3D neural in vitro model. Assessment of hd-CAV-2 vector efficacy was performed at different multiplicities of infection, by evaluating transgene expression and impact on cell viability, ultrastructural cellular organization and neuronal gene expression. Under optimized conditions, hd-CAV-2 transduction led to stable long-term transgene expression with minimal toxicity. hd-CAV-2 preferentially transduced neurons, whereas human adenovirus type 5 (HAdV5) showed increased tropism toward glial cells. This work demonstrates, in a physiologically relevant 3D model, that hd-CAV-2 vectors are efficient tools for gene delivery to human neurons, with stable long-term transgene expression and minimal cytotoxicity. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/gt.2015.75", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3525182", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3797661", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3773127", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2754968", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6798224", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "23"
      }
    ], 
    "name": "Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model", 
    "pagination": "86", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7d2cd301dc290ebe4cf8e607792a6a3a98a0b9979d0f0b698f5fa39b1d81c3f7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26181626"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9421525"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/gt.2015.75"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026275828"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/gt.2015.75", 
      "https://app.dimensions.ai/details/publication/pub.1026275828"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T00:05", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8695_00000434.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/gt201575"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/gt.2015.75'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/gt.2015.75'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/gt.2015.75'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/gt.2015.75'


 

This table displays all metadata directly associated to this object as RDF triples.

394 TRIPLES      21 PREDICATES      93 URIs      41 LITERALS      29 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/gt.2015.75 schema:about N10078eddc30c4876812fb1d19b4e2efd
2 N287ec6c506ee4488be82497de231d0a7
3 N299facef9e2948bdbb45bdc89f69f51f
4 N576c1fa46f40469fbdfb0b96c898ac68
5 N5e7d970d76af4a5e8563dd22b2baa588
6 N6a7296340fd141ccb6be71cfe8525cfb
7 N7a4a19fbfb6740efa935f865509d55d9
8 N8f7de4fbd58947e2a49f68f5abd5d2bc
9 N9a64fb506ee04729af6b7c54bb6dc5f0
10 Na167f6d6a0a44011ad18a5bc3dd6ecdb
11 Na2d21786d41c4b31ac06983a1779ad1a
12 Naae8aaf6f9914bde86738227b113a312
13 Nb3422f65f1be45beb248fbcd3c0b6557
14 Nbe7f29302ecb45fea1697404c7b787d9
15 Nc13d2da7442a482a9a48fa830ac3d023
16 Nc5bc5618718949f7b2d4cf44cdda4ab9
17 Nd23a5e76528940e88e19e8c5ffd7f36f
18 Nd60d6a60541c425590b7a67bc7491df6
19 Ne0bf5616ad8847369980955201494cac
20 Nf337a508f52842339dd4c885e6bfe4a7
21 anzsrc-for:11
22 anzsrc-for:1109
23 schema:author N811edaa5be6146a9aa21fd500fdb24b3
24 schema:citation sg:pub.10.1038/gt.2012.52
25 sg:pub.10.1038/gt.2014.92
26 sg:pub.10.1038/nature14600
27 sg:pub.10.1038/nm1113
28 sg:pub.10.1038/nmeth.2019
29 sg:pub.10.1038/nrm2236
30 sg:pub.10.1038/sj.gt.3302538
31 sg:pub.10.1038/sj.gt.3302779
32 sg:pub.10.1385/abab:133:1:9
33 sg:pub.10.1385/mb:34:2:247
34 sg:pub.10.2165/11533740-000000000-00000
35 https://app.dimensions.ai/details/publication/pub.1078138494
36 https://doi.org/10.1002/(sici)1097-0290(19961105)52:3<429::aid-bit9>3.0.co;2-m
37 https://doi.org/10.1002/biot.201400765
38 https://doi.org/10.1002/btpr.64
39 https://doi.org/10.1006/exnr.2001.7706
40 https://doi.org/10.1006/meth.2001.1262
41 https://doi.org/10.1006/mthe.2001.0263
42 https://doi.org/10.1016/j.bbadis.2009.02.009
43 https://doi.org/10.1016/j.bbadis.2009.08.014
44 https://doi.org/10.1016/j.nbd.2011.09.014
45 https://doi.org/10.1016/j.pneurobio.2008.05.003
46 https://doi.org/10.1016/j.ymeth.2012.03.005
47 https://doi.org/10.1016/j.ymthe.2005.10.008
48 https://doi.org/10.1074/jbc.m113.518365
49 https://doi.org/10.1074/jbc.m605316200
50 https://doi.org/10.1089/104303400750001417
51 https://doi.org/10.1089/ten.tea.2014.0079
52 https://doi.org/10.1096/fj.01-0321fje
53 https://doi.org/10.1096/fj.03-0438fje
54 https://doi.org/10.1126/science.1226625
55 https://doi.org/10.1128/jvi.03576-14
56 https://doi.org/10.1128/jvi.74.1.505-512.2000
57 https://doi.org/10.1128/jvi.74.22.10639-10649.2000
58 https://doi.org/10.1128/jvi.80.3.1487-1496.2006
59 https://doi.org/10.1371/journal.pone.0023212
60 https://doi.org/10.1371/journal.pone.0060342
61 https://doi.org/10.1371/journal.pone.0069808
62 https://doi.org/10.1371/journal.ppat.1000442
63 https://doi.org/10.2174/1874467210801010013
64 https://doi.org/10.2741/e607
65 https://doi.org/10.3390/v2092134
66 https://doi.org/10.4155/tde.10.50
67 https://doi.org/10.4155/tde.10.95
68 schema:datePublished 2016-01
69 schema:datePublishedReg 2016-01-01
70 schema:description Gene therapy is a promising approach with enormous potential for treatment of neurodegenerative disorders. Viral vectors derived from canine adenovirus type 2 (CAV-2) present attractive features for gene delivery strategies in the human brain, by preferentially transducing neurons, are capable of efficient axonal transport to afferent brain structures, have a 30-kb cloning capacity and have low innate and induced immunogenicity in preclinical tests. For clinical translation, in-depth preclinical evaluation of efficacy and safety in a human setting is primordial. Stem cell-derived human neural cells have a great potential as complementary tools by bridging the gap between animal models, which often diverge considerably from human phenotype, and clinical trials. Herein, we explore helper-dependent CAV-2 (hd-CAV-2) efficacy and safety for gene delivery in a human stem cell-derived 3D neural in vitro model. Assessment of hd-CAV-2 vector efficacy was performed at different multiplicities of infection, by evaluating transgene expression and impact on cell viability, ultrastructural cellular organization and neuronal gene expression. Under optimized conditions, hd-CAV-2 transduction led to stable long-term transgene expression with minimal toxicity. hd-CAV-2 preferentially transduced neurons, whereas human adenovirus type 5 (HAdV5) showed increased tropism toward glial cells. This work demonstrates, in a physiologically relevant 3D model, that hd-CAV-2 vectors are efficient tools for gene delivery to human neurons, with stable long-term transgene expression and minimal cytotoxicity.
71 schema:genre research_article
72 schema:inLanguage en
73 schema:isAccessibleForFree true
74 schema:isPartOf N86f72c64d2d84d939ea3b65023f4d605
75 Nd60f6c70fd6740dba4e9ed73f6ef864e
76 sg:journal.1105638
77 schema:name Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model
78 schema:pagination 86
79 schema:productId N0f7123e2c72c4cfd928cd34eef3c0326
80 N2e848940b9eb4d1f92880759a2ea5cbd
81 Naef3b4714b1a4ce0a7caf54a134344ca
82 Nb238fdea561442f6b55d5579eba72f86
83 Nb7ea4e11c89b48bea73db737182b1f7d
84 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026275828
85 https://doi.org/10.1038/gt.2015.75
86 schema:sdDatePublished 2019-04-11T00:05
87 schema:sdLicense https://scigraph.springernature.com/explorer/license/
88 schema:sdPublisher Nfee5ccd321414594a49ec194d149c2b3
89 schema:url https://www.nature.com/articles/gt201575
90 sgo:license sg:explorer/license/
91 sgo:sdDataset articles
92 rdf:type schema:ScholarlyArticle
93 N0944c5a29a774e38bf61517730a2aae7 rdf:first sg:person.01270140430.29
94 rdf:rest N1e32cfbc01314a1e918f14d1771a0e6d
95 N0f7123e2c72c4cfd928cd34eef3c0326 schema:name doi
96 schema:value 10.1038/gt.2015.75
97 rdf:type schema:PropertyValue
98 N10078eddc30c4876812fb1d19b4e2efd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Transgenes
100 rdf:type schema:DefinedTerm
101 N1c5621d83b6e447aa8e8f9a858ec4235 rdf:first sg:person.01043324530.18
102 rdf:rest Ne1328f2a135942d5a3188ef6b9add892
103 N1e32cfbc01314a1e918f14d1771a0e6d rdf:first sg:person.0677742110.53
104 rdf:rest N49b3734b17af40f592731ffdc6e3f376
105 N27aa936ddcd04a448bde775b9b6096ca rdf:first sg:person.014102654140.78
106 rdf:rest rdf:nil
107 N287ec6c506ee4488be82497de231d0a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Genetic Vectors
109 rdf:type schema:DefinedTerm
110 N299facef9e2948bdbb45bdc89f69f51f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Gene Transfer Techniques
112 rdf:type schema:DefinedTerm
113 N2e848940b9eb4d1f92880759a2ea5cbd schema:name dimensions_id
114 schema:value pub.1026275828
115 rdf:type schema:PropertyValue
116 N49b3734b17af40f592731ffdc6e3f376 rdf:first sg:person.01022143342.31
117 rdf:rest N1c5621d83b6e447aa8e8f9a858ec4235
118 N542a1ba7ea374acd976206f6c2bfe0d8 rdf:first sg:person.0616636744.58
119 rdf:rest N6661afea65ac401b9df3a3d1061fd969
120 N576c1fa46f40469fbdfb0b96c898ac68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Viral Tropism
122 rdf:type schema:DefinedTerm
123 N5e7d970d76af4a5e8563dd22b2baa588 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Neurons
125 rdf:type schema:DefinedTerm
126 N6661afea65ac401b9df3a3d1061fd969 rdf:first sg:person.0742214210.07
127 rdf:rest N0944c5a29a774e38bf61517730a2aae7
128 N68fb4952ce054f5281b992b7d92d9268 rdf:first sg:person.0651116711.42
129 rdf:rest N9dbe75efa32a4682842cb35deabe2b30
130 N6a7296340fd141ccb6be71cfe8525cfb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Cell Survival
132 rdf:type schema:DefinedTerm
133 N7a4a19fbfb6740efa935f865509d55d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Animals
135 rdf:type schema:DefinedTerm
136 N7b3461ccf28142abbf4708b8306097ef rdf:first sg:person.01107043614.07
137 rdf:rest N542a1ba7ea374acd976206f6c2bfe0d8
138 N811edaa5be6146a9aa21fd500fdb24b3 rdf:first sg:person.01304011123.53
139 rdf:rest N7b3461ccf28142abbf4708b8306097ef
140 N86f72c64d2d84d939ea3b65023f4d605 schema:issueNumber 1
141 rdf:type schema:PublicationIssue
142 N8f7de4fbd58947e2a49f68f5abd5d2bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Adenoviruses, Canine
144 rdf:type schema:DefinedTerm
145 N9a64fb506ee04729af6b7c54bb6dc5f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Genetic Therapy
147 rdf:type schema:DefinedTerm
148 N9dbe75efa32a4682842cb35deabe2b30 rdf:first sg:person.0747041465.15
149 rdf:rest N27aa936ddcd04a448bde775b9b6096ca
150 Na167f6d6a0a44011ad18a5bc3dd6ecdb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Central Nervous System
152 rdf:type schema:DefinedTerm
153 Na2d21786d41c4b31ac06983a1779ad1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Axonal Transport
155 rdf:type schema:DefinedTerm
156 Naae8aaf6f9914bde86738227b113a312 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Neural Stem Cells
158 rdf:type schema:DefinedTerm
159 Naef3b4714b1a4ce0a7caf54a134344ca schema:name pubmed_id
160 schema:value 26181626
161 rdf:type schema:PropertyValue
162 Nb238fdea561442f6b55d5579eba72f86 schema:name nlm_unique_id
163 schema:value 9421525
164 rdf:type schema:PropertyValue
165 Nb3422f65f1be45beb248fbcd3c0b6557 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Neuroglia
167 rdf:type schema:DefinedTerm
168 Nb7ea4e11c89b48bea73db737182b1f7d schema:name readcube_id
169 schema:value 7d2cd301dc290ebe4cf8e607792a6a3a98a0b9979d0f0b698f5fa39b1d81c3f7
170 rdf:type schema:PropertyValue
171 Nbe7f29302ecb45fea1697404c7b787d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Cloning, Molecular
173 rdf:type schema:DefinedTerm
174 Nc13d2da7442a482a9a48fa830ac3d023 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Humans
176 rdf:type schema:DefinedTerm
177 Nc5bc5618718949f7b2d4cf44cdda4ab9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Transduction, Genetic
179 rdf:type schema:DefinedTerm
180 Nd23a5e76528940e88e19e8c5ffd7f36f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Adenoviruses, Human
182 rdf:type schema:DefinedTerm
183 Nd60d6a60541c425590b7a67bc7491df6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Cell Differentiation
185 rdf:type schema:DefinedTerm
186 Nd60f6c70fd6740dba4e9ed73f6ef864e schema:volumeNumber 23
187 rdf:type schema:PublicationVolume
188 Ne0bf5616ad8847369980955201494cac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Disease Models, Animal
190 rdf:type schema:DefinedTerm
191 Ne1328f2a135942d5a3188ef6b9add892 rdf:first sg:person.01255571250.30
192 rdf:rest N68fb4952ce054f5281b992b7d92d9268
193 Nf337a508f52842339dd4c885e6bfe4a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Gene Expression Regulation
195 rdf:type schema:DefinedTerm
196 Nfee5ccd321414594a49ec194d149c2b3 schema:name Springer Nature - SN SciGraph project
197 rdf:type schema:Organization
198 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
199 schema:name Medical and Health Sciences
200 rdf:type schema:DefinedTerm
201 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
202 schema:name Neurosciences
203 rdf:type schema:DefinedTerm
204 sg:grant.2754968 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2015.75
205 rdf:type schema:MonetaryGrant
206 sg:grant.3525182 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2015.75
207 rdf:type schema:MonetaryGrant
208 sg:grant.3773127 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2015.75
209 rdf:type schema:MonetaryGrant
210 sg:grant.3797661 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2015.75
211 rdf:type schema:MonetaryGrant
212 sg:grant.6798224 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2015.75
213 rdf:type schema:MonetaryGrant
214 sg:journal.1105638 schema:issn 0969-7128
215 1476-5462
216 schema:name Gene Therapy
217 rdf:type schema:Periodical
218 sg:person.01022143342.31 schema:affiliation https://www.grid.ac/institutes/grid.121334.6
219 schema:familyName Salinas
220 schema:givenName S
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022143342.31
222 rdf:type schema:Person
223 sg:person.01043324530.18 schema:affiliation https://www.grid.ac/institutes/grid.7841.a
224 schema:familyName Saggio
225 schema:givenName I
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043324530.18
227 rdf:type schema:Person
228 sg:person.01107043614.07 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
229 schema:familyName Pinto
230 schema:givenName C
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107043614.07
232 rdf:type schema:Person
233 sg:person.01255571250.30 schema:affiliation https://www.grid.ac/institutes/grid.83440.3b
234 schema:familyName Schiavo
235 schema:givenName G
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255571250.30
237 rdf:type schema:Person
238 sg:person.01270140430.29 schema:affiliation https://www.grid.ac/institutes/grid.7841.a
239 schema:familyName Piersanti
240 schema:givenName S
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270140430.29
242 rdf:type schema:Person
243 sg:person.01304011123.53 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
244 schema:familyName Simão
245 schema:givenName D
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304011123.53
247 rdf:type schema:Person
248 sg:person.014102654140.78 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
249 schema:familyName Alves
250 schema:givenName P M
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014102654140.78
252 rdf:type schema:Person
253 sg:person.0616636744.58 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
254 schema:familyName Fernandes
255 schema:givenName P
256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616636744.58
257 rdf:type schema:Person
258 sg:person.0651116711.42 schema:affiliation https://www.grid.ac/institutes/grid.121334.6
259 schema:familyName Kremer
260 schema:givenName E J
261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0651116711.42
262 rdf:type schema:Person
263 sg:person.0677742110.53 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
264 schema:familyName Collinson
265 schema:givenName L M
266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677742110.53
267 rdf:type schema:Person
268 sg:person.0742214210.07 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
269 schema:familyName Peddie
270 schema:givenName C J
271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742214210.07
272 rdf:type schema:Person
273 sg:person.0747041465.15 schema:affiliation https://www.grid.ac/institutes/grid.10772.33
274 schema:familyName Brito
275 schema:givenName C
276 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747041465.15
277 rdf:type schema:Person
278 sg:pub.10.1038/gt.2012.52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003225579
279 https://doi.org/10.1038/gt.2012.52
280 rdf:type schema:CreativeWork
281 sg:pub.10.1038/gt.2014.92 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046055806
282 https://doi.org/10.1038/gt.2014.92
283 rdf:type schema:CreativeWork
284 sg:pub.10.1038/nature14600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053182885
285 https://doi.org/10.1038/nature14600
286 rdf:type schema:CreativeWork
287 sg:pub.10.1038/nm1113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037922659
288 https://doi.org/10.1038/nm1113
289 rdf:type schema:CreativeWork
290 sg:pub.10.1038/nmeth.2019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026706357
291 https://doi.org/10.1038/nmeth.2019
292 rdf:type schema:CreativeWork
293 sg:pub.10.1038/nrm2236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015693164
294 https://doi.org/10.1038/nrm2236
295 rdf:type schema:CreativeWork
296 sg:pub.10.1038/sj.gt.3302538 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022297273
297 https://doi.org/10.1038/sj.gt.3302538
298 rdf:type schema:CreativeWork
299 sg:pub.10.1038/sj.gt.3302779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011750309
300 https://doi.org/10.1038/sj.gt.3302779
301 rdf:type schema:CreativeWork
302 sg:pub.10.1385/abab:133:1:9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022155349
303 https://doi.org/10.1385/abab:133:1:9
304 rdf:type schema:CreativeWork
305 sg:pub.10.1385/mb:34:2:247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005939199
306 https://doi.org/10.1385/mb:34:2:247
307 rdf:type schema:CreativeWork
308 sg:pub.10.2165/11533740-000000000-00000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026351862
309 https://doi.org/10.2165/11533740-000000000-00000
310 rdf:type schema:CreativeWork
311 https://app.dimensions.ai/details/publication/pub.1078138494 schema:CreativeWork
312 https://doi.org/10.1002/(sici)1097-0290(19961105)52:3<429::aid-bit9>3.0.co;2-m schema:sameAs https://app.dimensions.ai/details/publication/pub.1008260782
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1002/biot.201400765 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027018540
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1002/btpr.64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006820929
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1006/exnr.2001.7706 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023213170
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1006/meth.2001.1262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027621591
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1006/mthe.2001.0263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043354276
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1016/j.bbadis.2009.02.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003314348
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1016/j.bbadis.2009.08.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047597521
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1016/j.nbd.2011.09.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032486602
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1016/j.pneurobio.2008.05.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018762161
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1016/j.ymeth.2012.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044890446
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1016/j.ymthe.2005.10.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025243513
335 rdf:type schema:CreativeWork
336 https://doi.org/10.1074/jbc.m113.518365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046652305
337 rdf:type schema:CreativeWork
338 https://doi.org/10.1074/jbc.m605316200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029568633
339 rdf:type schema:CreativeWork
340 https://doi.org/10.1089/104303400750001417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059202084
341 rdf:type schema:CreativeWork
342 https://doi.org/10.1089/ten.tea.2014.0079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059316569
343 rdf:type schema:CreativeWork
344 https://doi.org/10.1096/fj.01-0321fje schema:sameAs https://app.dimensions.ai/details/publication/pub.1018968665
345 rdf:type schema:CreativeWork
346 https://doi.org/10.1096/fj.03-0438fje schema:sameAs https://app.dimensions.ai/details/publication/pub.1017721166
347 rdf:type schema:CreativeWork
348 https://doi.org/10.1126/science.1226625 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062466980
349 rdf:type schema:CreativeWork
350 https://doi.org/10.1128/jvi.03576-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025872939
351 rdf:type schema:CreativeWork
352 https://doi.org/10.1128/jvi.74.1.505-512.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017312190
353 rdf:type schema:CreativeWork
354 https://doi.org/10.1128/jvi.74.22.10639-10649.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033541280
355 rdf:type schema:CreativeWork
356 https://doi.org/10.1128/jvi.80.3.1487-1496.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025110102
357 rdf:type schema:CreativeWork
358 https://doi.org/10.1371/journal.pone.0023212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006205975
359 rdf:type schema:CreativeWork
360 https://doi.org/10.1371/journal.pone.0060342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000868130
361 rdf:type schema:CreativeWork
362 https://doi.org/10.1371/journal.pone.0069808 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019848936
363 rdf:type schema:CreativeWork
364 https://doi.org/10.1371/journal.ppat.1000442 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000944773
365 rdf:type schema:CreativeWork
366 https://doi.org/10.2174/1874467210801010013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069234160
367 rdf:type schema:CreativeWork
368 https://doi.org/10.2741/e607 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070918084
369 rdf:type schema:CreativeWork
370 https://doi.org/10.3390/v2092134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040570415
371 rdf:type schema:CreativeWork
372 https://doi.org/10.4155/tde.10.50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023556570
373 rdf:type schema:CreativeWork
374 https://doi.org/10.4155/tde.10.95 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020490619
375 rdf:type schema:CreativeWork
376 https://www.grid.ac/institutes/grid.10772.33 schema:alternateName Universidade Nova de Lisboa
377 schema:name Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
378 iBET—Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal
379 rdf:type schema:Organization
380 https://www.grid.ac/institutes/grid.121334.6 schema:alternateName University of Montpellier
381 schema:name Institut de Génétique Moléculaire de Montpellier, Montpellier, France
382 Université Montpellier, Montpellier, France
383 rdf:type schema:Organization
384 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
385 schema:name The Francis Crick Institute, Lincoln's Inn Fields Laboratory, London, UK
386 rdf:type schema:Organization
387 https://www.grid.ac/institutes/grid.7841.a schema:alternateName Sapienza University of Rome
388 schema:name Dipartimento di Biologia e Biotecnologie ‘Charles Darwin’, Università di Roma La Sapienza, Rome, Italy
389 Istituto Pasteur Fondazione Cenci Bolognetti, Università di Roma La Sapienza, Rome, Italy
390 Istituto di Biologia e Patologia Molecolari del CNR, Università di Roma La Sapienza, Rome, Italy
391 rdf:type schema:Organization
392 https://www.grid.ac/institutes/grid.83440.3b schema:alternateName University College London
393 schema:name Sobell Department of Motor Neuroscience and Movement Disorders, Institute of Neurology, University College London, London, UK
394 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...