The Tol2 transposon system mediates the genetic engineering of T-cells with CD19-specific chimeric antigen receptors for B-cell malignancies View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-11-27

AUTHORS

T Tsukahara, N Iwase, K Kawakami, M Iwasaki, C Yamamoto, K Ohmine, R Uchibori, T Teruya, H Ido, Y Saga, M Urabe, H Mizukami, A Kume, M Nakamura, R Brentjens, K Ozawa

ABSTRACT

Engineered T-cell therapy using a CD19-specific chimeric antigen receptor (CD19-CAR) is a promising strategy for the treatment of advanced B-cell malignancies. Gene transfer of CARs to T-cells has widely relied on retroviral vectors, but transposon-based gene transfer has recently emerged as a suitable nonviral method to mediate stable transgene expression. The advantages of transposon vectors compared with viral vectors include their simplicity and cost-effectiveness. We used the Tol2 transposon system to stably transfer CD19-CAR into human T-cells. Normal human peripheral blood lymphocytes were co-nucleofected with the Tol2 transposon donor plasmid carrying CD19-CAR and the transposase expression plasmid and were selectively propagated on NIH3T3 cells expressing human CD19. Expanded CD3+ T-cells with stable and high-level transgene expression (~95%) produced interferon-γ upon stimulation with CD19 and specifically lysed Raji cells, a CD19+ human B-cell lymphoma cell line. Adoptive transfer of these T-cells suppressed tumor progression in Raji tumor-bearing Rag2−/−γc−/− immunodeficient mice compared with control mice. These results demonstrate that the Tol2 transposon system could be used to express CD19-CAR in genetically engineered T-cells for the treatment of refractory B-cell malignancies. More... »

PAGES

209-215

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/gt.2014.104

DOI

http://dx.doi.org/10.1038/gt.2014.104

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005320016

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25427612


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD19", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coculture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Transposable Elements", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunotherapy, Adoptive", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphoma, B-Cell", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred BALB C", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Knockout", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "NIH 3T3 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Antigen, T-Cell", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
            "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsukahara", 
        "givenName": "T", 
        "id": "sg:person.01172111242.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172111242.59"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Iwase", 
        "givenName": "N", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.288127.6", 
          "name": [
            "Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kawakami", 
        "givenName": "K", 
        "id": "sg:person.01030362062.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030362062.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.288127.6", 
          "name": [
            "Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Iwasaki", 
        "givenName": "M", 
        "id": "sg:person.01313227340.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313227340.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
            "Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamoto", 
        "givenName": "C", 
        "id": "sg:person.01247654435.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247654435.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan", 
            "Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ohmine", 
        "givenName": "K", 
        "id": "sg:person.01144457051.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144457051.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
            "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Uchibori", 
        "givenName": "R", 
        "id": "sg:person.01123776042.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123776042.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Teruya", 
        "givenName": "T", 
        "id": "sg:person.01161630306.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161630306.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ido", 
        "givenName": "H", 
        "id": "sg:person.01113515106.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113515106.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
            "Department of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saga", 
        "givenName": "Y", 
        "id": "sg:person.0611710645.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611710645.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Urabe", 
        "givenName": "M", 
        "id": "sg:person.01337270101.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337270101.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mizukami", 
        "givenName": "H", 
        "id": "sg:person.0613664001.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613664001.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kume", 
        "givenName": "A", 
        "id": "sg:person.0672430713.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672430713.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Human Gene Sciences Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.265073.5", 
          "name": [
            "Human Gene Sciences Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakamura", 
        "givenName": "M", 
        "id": "sg:person.011030722564.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011030722564.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA", 
          "id": "http://www.grid.ac/institutes/grid.51462.34", 
          "name": [
            "Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brentjens", 
        "givenName": "R", 
        "id": "sg:person.01263050511.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263050511.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410804.9", 
          "name": [
            "Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan", 
            "Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan", 
            "Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ozawa", 
        "givenName": "K", 
        "id": "sg:person.013136320052.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013136320052.97"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/74455", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011466374", 
          "https://doi.org/10.1038/74455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc3179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052351170", 
          "https://doi.org/10.1038/nrc3179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1472-6750-10-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002841635", 
          "https://doi.org/10.1186/1472-6750-10-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni.2762", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046343330", 
          "https://doi.org/10.1038/ni.2762"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02994009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040745127", 
          "https://doi.org/10.1007/bf02994009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-10-477", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051515093", 
          "https://doi.org/10.1186/1471-2164-10-477"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2007-8-s1-s7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006435958", 
          "https://doi.org/10.1186/gb-2007-8-s1-s7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrclinonc.2013.46", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045630180", 
          "https://doi.org/10.1038/nrclinonc.2013.46"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/gt.2011.40", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034274955", 
          "https://doi.org/10.1038/gt.2011.40"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/gt.2009.54", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028629257", 
          "https://doi.org/10.1038/gt.2009.54"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-11-27", 
    "datePublishedReg": "2014-11-27", 
    "description": "Engineered T-cell therapy using a CD19-specific chimeric antigen receptor (CD19-CAR) is a promising strategy for the treatment of advanced B-cell malignancies. Gene transfer of CARs to T-cells has widely relied on retroviral vectors, but transposon-based gene transfer has recently emerged as a suitable nonviral method to mediate stable transgene expression. The advantages of transposon vectors compared with viral vectors include their simplicity and cost-effectiveness. We used the Tol2 transposon system to stably transfer CD19-CAR into human T-cells. Normal human peripheral blood lymphocytes were co-nucleofected with the Tol2 transposon donor plasmid carrying CD19-CAR and the transposase expression plasmid and were selectively propagated on NIH3T3 cells expressing human CD19. Expanded CD3+ T-cells with stable and high-level transgene expression (~95%) produced interferon-\u03b3 upon stimulation with CD19 and specifically lysed Raji cells, a CD19+ human B-cell lymphoma cell line. Adoptive transfer of these T-cells suppressed tumor progression in Raji tumor-bearing Rag2\u2212/\u2212\u03b3c\u2212/\u2212 immunodeficient mice compared with control mice. These results demonstrate that the Tol2 transposon system could be used to express CD19-CAR in genetically engineered T-cells for the treatment of refractory B-cell malignancies.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/gt.2014.104", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6123438", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2438793", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6050094", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6048023", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6082931", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6141763", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "22"
      }
    ], 
    "keywords": [
      "B-cell malignancies", 
      "CD19-specific chimeric antigen receptor", 
      "chimeric antigen receptor", 
      "T cells", 
      "CD19-CAR", 
      "advanced B-cell malignancies", 
      "normal human peripheral blood lymphocytes", 
      "refractory B-cell malignancies", 
      "antigen receptor", 
      "human peripheral blood lymphocytes", 
      "Engineered T cell therapies", 
      "human B-cell lymphoma cell lines", 
      "T-cell therapy", 
      "peripheral blood lymphocytes", 
      "B-cell lymphoma cell lines", 
      "human T cells", 
      "adoptive transfer", 
      "control mice", 
      "blood lymphocytes", 
      "lymphoma cell lines", 
      "human CD19", 
      "immunodeficient mice", 
      "tumor progression", 
      "transgene expression", 
      "Raji cells", 
      "CD19", 
      "malignancy", 
      "gene transfer", 
      "cell lines", 
      "mice", 
      "viral vectors", 
      "receptors", 
      "treatment", 
      "retroviral vectors", 
      "expression plasmid", 
      "promising strategy", 
      "stable transgene expression", 
      "cells", 
      "high-level transgene expression", 
      "CD3", 
      "lymphocytes", 
      "therapy", 
      "expression", 
      "interferon", 
      "NIH3T3 cells", 
      "progression", 
      "stimulation", 
      "Raji", 
      "nonviral methods", 
      "transposon system", 
      "plasmid", 
      "transposon donor plasmid", 
      "strategies", 
      "lines", 
      "vector", 
      "results", 
      "transposon vector", 
      "system", 
      "donor plasmid", 
      "Tol2 transposon system", 
      "transfer", 
      "method", 
      "genetic engineering", 
      "advantages", 
      "transposon", 
      "cars", 
      "simplicity", 
      "engineering"
    ], 
    "name": "The Tol2 transposon system mediates the genetic engineering of T-cells with CD19-specific chimeric antigen receptors for B-cell malignancies", 
    "pagination": "209-215", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005320016"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/gt.2014.104"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25427612"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/gt.2014.104", 
      "https://app.dimensions.ai/details/publication/pub.1005320016"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T15:56", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_627.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/gt.2014.104"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/gt.2014.104'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/gt.2014.104'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/gt.2014.104'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/gt.2014.104'


 

This table displays all metadata directly associated to this object as RDF triples.

376 TRIPLES      21 PREDICATES      122 URIs      104 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/gt.2014.104 schema:about N1452bd3fe31d49f5bfa58541f913933b
2 N1d6ab2fceb26497386639aaa5e81c070
3 N239487f21c944081a11247e783fde1e5
4 N3a414ff680fe455183196eca24f91022
5 N4374a6d8edfd4738aeebb0994d7fe0a9
6 N47c6dc3a1030440fb23a233b623c9d34
7 N50458f18e30042308a09b32fc88e88cc
8 N511d4ea9abd9481f917746ccc11e0963
9 N66e763aa22c64e6a88a91a971d560480
10 N8287f442879c458282fc23ba7926796a
11 N9241f1e6f91c444b8de54b2e4e5b550f
12 N9a3f51997ffe411ebff4ba18029b8807
13 Nae4ff4d067a74775b3ef9aa50302a130
14 Nd4cc6092ab0745ba86dc23042b00a452
15 Ndf05f9512fd6466684e34f55dcb9dd96
16 Ne38e7b7ddef14059b065fd162f734dff
17 Nea312520040e437591ca448c581f8c32
18 Nec583d9c26c84b51882f2305628d6881
19 Nfd6dc595d7194fa7a71d221d51c8107d
20 anzsrc-for:11
21 anzsrc-for:1107
22 schema:author N1e6010f597a247c0aa295336f73bc7a9
23 schema:citation sg:pub.10.1007/bf02994009
24 sg:pub.10.1038/74455
25 sg:pub.10.1038/gt.2009.54
26 sg:pub.10.1038/gt.2011.40
27 sg:pub.10.1038/ni.2762
28 sg:pub.10.1038/nrc3179
29 sg:pub.10.1038/nrclinonc.2013.46
30 sg:pub.10.1186/1471-2164-10-477
31 sg:pub.10.1186/1472-6750-10-3
32 sg:pub.10.1186/gb-2007-8-s1-s7
33 schema:datePublished 2014-11-27
34 schema:datePublishedReg 2014-11-27
35 schema:description Engineered T-cell therapy using a CD19-specific chimeric antigen receptor (CD19-CAR) is a promising strategy for the treatment of advanced B-cell malignancies. Gene transfer of CARs to T-cells has widely relied on retroviral vectors, but transposon-based gene transfer has recently emerged as a suitable nonviral method to mediate stable transgene expression. The advantages of transposon vectors compared with viral vectors include their simplicity and cost-effectiveness. We used the Tol2 transposon system to stably transfer CD19-CAR into human T-cells. Normal human peripheral blood lymphocytes were co-nucleofected with the Tol2 transposon donor plasmid carrying CD19-CAR and the transposase expression plasmid and were selectively propagated on NIH3T3 cells expressing human CD19. Expanded CD3+ T-cells with stable and high-level transgene expression (~95%) produced interferon-γ upon stimulation with CD19 and specifically lysed Raji cells, a CD19+ human B-cell lymphoma cell line. Adoptive transfer of these T-cells suppressed tumor progression in Raji tumor-bearing Rag2−/−γc−/− immunodeficient mice compared with control mice. These results demonstrate that the Tol2 transposon system could be used to express CD19-CAR in genetically engineered T-cells for the treatment of refractory B-cell malignancies.
36 schema:genre article
37 schema:isAccessibleForFree true
38 schema:isPartOf N60771b209f304ca8a6375038b3d9911d
39 Ned1723847cb34212a3839ad8dea39745
40 sg:journal.1105638
41 schema:keywords B-cell lymphoma cell lines
42 B-cell malignancies
43 CD19
44 CD19-CAR
45 CD19-specific chimeric antigen receptor
46 CD3
47 Engineered T cell therapies
48 NIH3T3 cells
49 Raji
50 Raji cells
51 T cells
52 T-cell therapy
53 Tol2 transposon system
54 adoptive transfer
55 advanced B-cell malignancies
56 advantages
57 antigen receptor
58 blood lymphocytes
59 cars
60 cell lines
61 cells
62 chimeric antigen receptor
63 control mice
64 donor plasmid
65 engineering
66 expression
67 expression plasmid
68 gene transfer
69 genetic engineering
70 high-level transgene expression
71 human B-cell lymphoma cell lines
72 human CD19
73 human T cells
74 human peripheral blood lymphocytes
75 immunodeficient mice
76 interferon
77 lines
78 lymphocytes
79 lymphoma cell lines
80 malignancy
81 method
82 mice
83 nonviral methods
84 normal human peripheral blood lymphocytes
85 peripheral blood lymphocytes
86 plasmid
87 progression
88 promising strategy
89 receptors
90 refractory B-cell malignancies
91 results
92 retroviral vectors
93 simplicity
94 stable transgene expression
95 stimulation
96 strategies
97 system
98 therapy
99 transfer
100 transgene expression
101 transposon
102 transposon donor plasmid
103 transposon system
104 transposon vector
105 treatment
106 tumor progression
107 vector
108 viral vectors
109 schema:name The Tol2 transposon system mediates the genetic engineering of T-cells with CD19-specific chimeric antigen receptors for B-cell malignancies
110 schema:pagination 209-215
111 schema:productId N0d8d372f3615464d8aa7c17f6e721d4c
112 N6871446e7d594c48a8ee46f8df5ba009
113 Ncf460b25aead4ce4a320d0516aa7f546
114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005320016
115 https://doi.org/10.1038/gt.2014.104
116 schema:sdDatePublished 2022-09-02T15:56
117 schema:sdLicense https://scigraph.springernature.com/explorer/license/
118 schema:sdPublisher N530d8468eac244798994d2b11cd860e6
119 schema:url https://doi.org/10.1038/gt.2014.104
120 sgo:license sg:explorer/license/
121 sgo:sdDataset articles
122 rdf:type schema:ScholarlyArticle
123 N09d61e40f1fc43e88328123c78b18ac0 rdf:first sg:person.0613664001.27
124 rdf:rest N227b5d1108e74c75b4f266cccae98071
125 N0d8d372f3615464d8aa7c17f6e721d4c schema:name pubmed_id
126 schema:value 25427612
127 rdf:type schema:PropertyValue
128 N10babb45e7064d4a8ed564111e444867 rdf:first sg:person.013136320052.97
129 rdf:rest rdf:nil
130 N1452bd3fe31d49f5bfa58541f913933b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Mice, Inbred BALB C
132 rdf:type schema:DefinedTerm
133 N1d6ab2fceb26497386639aaa5e81c070 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Immunotherapy, Adoptive
135 rdf:type schema:DefinedTerm
136 N1e6010f597a247c0aa295336f73bc7a9 rdf:first sg:person.01172111242.59
137 rdf:rest N479a05cd92e244b8b3e120551807258e
138 N227b5d1108e74c75b4f266cccae98071 rdf:first sg:person.0672430713.98
139 rdf:rest Na5b0a5ba414b4107b6bacca83cf8652b
140 N239487f21c944081a11247e783fde1e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name NIH 3T3 Cells
142 rdf:type schema:DefinedTerm
143 N3a414ff680fe455183196eca24f91022 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Lymphoma, B-Cell
145 rdf:type schema:DefinedTerm
146 N4374a6d8edfd4738aeebb0994d7fe0a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Mice, Knockout
148 rdf:type schema:DefinedTerm
149 N46f89867f96d485e86c62cfeb3c7ad08 rdf:first sg:person.0611710645.56
150 rdf:rest N99129959a6b6476b8abbdf6226163229
151 N479a05cd92e244b8b3e120551807258e rdf:first N89f41097033c4416ae9e76809a7edde4
152 rdf:rest Nf6111f3aad034775b5bc8b74db8ff9a3
153 N47c6dc3a1030440fb23a233b623c9d34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Cell Line, Tumor
155 rdf:type schema:DefinedTerm
156 N50458f18e30042308a09b32fc88e88cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Antigens, CD19
158 rdf:type schema:DefinedTerm
159 N511d4ea9abd9481f917746ccc11e0963 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Genetic Engineering
161 rdf:type schema:DefinedTerm
162 N530d8468eac244798994d2b11cd860e6 schema:name Springer Nature - SN SciGraph project
163 rdf:type schema:Organization
164 N60771b209f304ca8a6375038b3d9911d schema:issueNumber 2
165 rdf:type schema:PublicationIssue
166 N66e763aa22c64e6a88a91a971d560480 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Molecular Sequence Data
168 rdf:type schema:DefinedTerm
169 N686f0ac66c4046138923333ca4f0ad02 rdf:first sg:person.01113515106.13
170 rdf:rest N46f89867f96d485e86c62cfeb3c7ad08
171 N6871446e7d594c48a8ee46f8df5ba009 schema:name doi
172 schema:value 10.1038/gt.2014.104
173 rdf:type schema:PropertyValue
174 N8287f442879c458282fc23ba7926796a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Neoplasm Transplantation
176 rdf:type schema:DefinedTerm
177 N876c4020b1a1482ab1c6cb2e63293fd0 rdf:first sg:person.01144457051.28
178 rdf:rest Na10b4bd7fb9a4d91a74de6bc8cf6a0ba
179 N89f41097033c4416ae9e76809a7edde4 schema:affiliation grid-institutes:grid.410804.9
180 schema:familyName Iwase
181 schema:givenName N
182 rdf:type schema:Person
183 N9241f1e6f91c444b8de54b2e4e5b550f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Genetic Therapy
185 rdf:type schema:DefinedTerm
186 N99129959a6b6476b8abbdf6226163229 rdf:first sg:person.01337270101.39
187 rdf:rest N09d61e40f1fc43e88328123c78b18ac0
188 N9a3f51997ffe411ebff4ba18029b8807 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name T-Lymphocytes
190 rdf:type schema:DefinedTerm
191 Na10b4bd7fb9a4d91a74de6bc8cf6a0ba rdf:first sg:person.01123776042.03
192 rdf:rest Nf9a31018b22c4b059f8722054bda0209
193 Na5b0a5ba414b4107b6bacca83cf8652b rdf:first sg:person.011030722564.57
194 rdf:rest Nf12468b693974d03af8b83bf6b928424
195 Nae4ff4d067a74775b3ef9aa50302a130 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
196 schema:name Receptors, Antigen, T-Cell
197 rdf:type schema:DefinedTerm
198 Ncd9afd61fb2647fe86a177df184642b5 rdf:first sg:person.01313227340.74
199 rdf:rest Ndfb1ffacb52e42bcb6758cf818a54171
200 Ncf460b25aead4ce4a320d0516aa7f546 schema:name dimensions_id
201 schema:value pub.1005320016
202 rdf:type schema:PropertyValue
203 Nd4cc6092ab0745ba86dc23042b00a452 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
204 schema:name Mice
205 rdf:type schema:DefinedTerm
206 Ndf05f9512fd6466684e34f55dcb9dd96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
207 schema:name DNA Transposable Elements
208 rdf:type schema:DefinedTerm
209 Ndfb1ffacb52e42bcb6758cf818a54171 rdf:first sg:person.01247654435.18
210 rdf:rest N876c4020b1a1482ab1c6cb2e63293fd0
211 Ne38e7b7ddef14059b065fd162f734dff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Recombinant Fusion Proteins
213 rdf:type schema:DefinedTerm
214 Nea312520040e437591ca448c581f8c32 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
215 schema:name Humans
216 rdf:type schema:DefinedTerm
217 Nec583d9c26c84b51882f2305628d6881 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
218 schema:name Coculture Techniques
219 rdf:type schema:DefinedTerm
220 Ned1723847cb34212a3839ad8dea39745 schema:volumeNumber 22
221 rdf:type schema:PublicationVolume
222 Nf12468b693974d03af8b83bf6b928424 rdf:first sg:person.01263050511.77
223 rdf:rest N10babb45e7064d4a8ed564111e444867
224 Nf6111f3aad034775b5bc8b74db8ff9a3 rdf:first sg:person.01030362062.32
225 rdf:rest Ncd9afd61fb2647fe86a177df184642b5
226 Nf9a31018b22c4b059f8722054bda0209 rdf:first sg:person.01161630306.60
227 rdf:rest N686f0ac66c4046138923333ca4f0ad02
228 Nfd6dc595d7194fa7a71d221d51c8107d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
229 schema:name Animals
230 rdf:type schema:DefinedTerm
231 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
232 schema:name Medical and Health Sciences
233 rdf:type schema:DefinedTerm
234 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
235 schema:name Immunology
236 rdf:type schema:DefinedTerm
237 sg:grant.2438793 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2014.104
238 rdf:type schema:MonetaryGrant
239 sg:grant.6048023 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2014.104
240 rdf:type schema:MonetaryGrant
241 sg:grant.6050094 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2014.104
242 rdf:type schema:MonetaryGrant
243 sg:grant.6082931 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2014.104
244 rdf:type schema:MonetaryGrant
245 sg:grant.6123438 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2014.104
246 rdf:type schema:MonetaryGrant
247 sg:grant.6141763 http://pending.schema.org/fundedItem sg:pub.10.1038/gt.2014.104
248 rdf:type schema:MonetaryGrant
249 sg:journal.1105638 schema:issn 0969-7128
250 1476-5462
251 schema:name Gene Therapy
252 schema:publisher Springer Nature
253 rdf:type schema:Periodical
254 sg:person.01030362062.32 schema:affiliation grid-institutes:grid.288127.6
255 schema:familyName Kawakami
256 schema:givenName K
257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030362062.32
258 rdf:type schema:Person
259 sg:person.011030722564.57 schema:affiliation grid-institutes:grid.265073.5
260 schema:familyName Nakamura
261 schema:givenName M
262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011030722564.57
263 rdf:type schema:Person
264 sg:person.01113515106.13 schema:affiliation grid-institutes:grid.410804.9
265 schema:familyName Ido
266 schema:givenName H
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113515106.13
268 rdf:type schema:Person
269 sg:person.01123776042.03 schema:affiliation grid-institutes:grid.410804.9
270 schema:familyName Uchibori
271 schema:givenName R
272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123776042.03
273 rdf:type schema:Person
274 sg:person.01144457051.28 schema:affiliation grid-institutes:grid.410804.9
275 schema:familyName Ohmine
276 schema:givenName K
277 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144457051.28
278 rdf:type schema:Person
279 sg:person.01161630306.60 schema:affiliation grid-institutes:grid.410804.9
280 schema:familyName Teruya
281 schema:givenName T
282 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161630306.60
283 rdf:type schema:Person
284 sg:person.01172111242.59 schema:affiliation grid-institutes:grid.410804.9
285 schema:familyName Tsukahara
286 schema:givenName T
287 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172111242.59
288 rdf:type schema:Person
289 sg:person.01247654435.18 schema:affiliation grid-institutes:grid.410804.9
290 schema:familyName Yamamoto
291 schema:givenName C
292 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247654435.18
293 rdf:type schema:Person
294 sg:person.01263050511.77 schema:affiliation grid-institutes:grid.51462.34
295 schema:familyName Brentjens
296 schema:givenName R
297 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263050511.77
298 rdf:type schema:Person
299 sg:person.01313227340.74 schema:affiliation grid-institutes:grid.288127.6
300 schema:familyName Iwasaki
301 schema:givenName M
302 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313227340.74
303 rdf:type schema:Person
304 sg:person.013136320052.97 schema:affiliation grid-institutes:grid.410804.9
305 schema:familyName Ozawa
306 schema:givenName K
307 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013136320052.97
308 rdf:type schema:Person
309 sg:person.01337270101.39 schema:affiliation grid-institutes:grid.410804.9
310 schema:familyName Urabe
311 schema:givenName M
312 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337270101.39
313 rdf:type schema:Person
314 sg:person.0611710645.56 schema:affiliation grid-institutes:grid.410804.9
315 schema:familyName Saga
316 schema:givenName Y
317 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611710645.56
318 rdf:type schema:Person
319 sg:person.0613664001.27 schema:affiliation grid-institutes:grid.410804.9
320 schema:familyName Mizukami
321 schema:givenName H
322 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613664001.27
323 rdf:type schema:Person
324 sg:person.0672430713.98 schema:affiliation grid-institutes:grid.410804.9
325 schema:familyName Kume
326 schema:givenName A
327 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672430713.98
328 rdf:type schema:Person
329 sg:pub.10.1007/bf02994009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040745127
330 https://doi.org/10.1007/bf02994009
331 rdf:type schema:CreativeWork
332 sg:pub.10.1038/74455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011466374
333 https://doi.org/10.1038/74455
334 rdf:type schema:CreativeWork
335 sg:pub.10.1038/gt.2009.54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028629257
336 https://doi.org/10.1038/gt.2009.54
337 rdf:type schema:CreativeWork
338 sg:pub.10.1038/gt.2011.40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034274955
339 https://doi.org/10.1038/gt.2011.40
340 rdf:type schema:CreativeWork
341 sg:pub.10.1038/ni.2762 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046343330
342 https://doi.org/10.1038/ni.2762
343 rdf:type schema:CreativeWork
344 sg:pub.10.1038/nrc3179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052351170
345 https://doi.org/10.1038/nrc3179
346 rdf:type schema:CreativeWork
347 sg:pub.10.1038/nrclinonc.2013.46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045630180
348 https://doi.org/10.1038/nrclinonc.2013.46
349 rdf:type schema:CreativeWork
350 sg:pub.10.1186/1471-2164-10-477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051515093
351 https://doi.org/10.1186/1471-2164-10-477
352 rdf:type schema:CreativeWork
353 sg:pub.10.1186/1472-6750-10-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002841635
354 https://doi.org/10.1186/1472-6750-10-3
355 rdf:type schema:CreativeWork
356 sg:pub.10.1186/gb-2007-8-s1-s7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006435958
357 https://doi.org/10.1186/gb-2007-8-s1-s7
358 rdf:type schema:CreativeWork
359 grid-institutes:grid.265073.5 schema:alternateName Human Gene Sciences Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan
360 schema:name Human Gene Sciences Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan
361 rdf:type schema:Organization
362 grid-institutes:grid.288127.6 schema:alternateName Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan
363 schema:name Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan
364 rdf:type schema:Organization
365 grid-institutes:grid.410804.9 schema:alternateName Department of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Tochigi, Japan
366 Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
367 Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
368 Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan
369 schema:name Department of Obstetrics and Gynecology, Jichi Medical University, Shimotsuke, Tochigi, Japan
370 Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
371 Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
372 Division of Immuno-Gene and Cell Therapy (Takara Bio), Jichi Medical University, Shimotsuke, Tochigi, Japan
373 rdf:type schema:Organization
374 grid-institutes:grid.51462.34 schema:alternateName Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA
375 schema:name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA
376 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...