A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-09-08

AUTHORS

J Jang, K Yoon, D W Hwang, D S Lee, S Kim

ABSTRACT

Gene transfer to the early-stage embryonic brain using the ultrasound image-guided gene delivery (UIGD) technique has proven to be valuable for investigating brain development. Thus far, this technology has been restricted to the study of embryonic neurogenesis. When this technique is designed to be employed for the study in adult animals, a long-term stable gene expression will be required. We attempted to develop a retroviral vector suitable for expressing exogenous genes in the brains of postnatal and adult mice in the context of the UIGD technique. Retroviral vectors containing four different long terminal repeats (LTRs) (each from Moloney murine leukemia virus (MoMLV), murine stem cell virus (MSCV), myeloproliferative sarcoma virus (MPSV) and spleen focus-forming virus (SFFV)) were compared using the well-known CE vector having the EF1α internal promoter as a control. The MS vector containing MSCV LTR produced a higher viral titer and a higher level of gene expression than other vectors including CE. The MS vector drove the gene expression in cultured neural stem cells for 3 weeks. Furthermore, the MS vector could efficiently deliver the gene to the mouse central nervous system, as transgene expression was found in various regions of the brains and spinal cords as well as in all major neural cell types. The data from an in vivo luciferase imaging analysis showed that the gene expression from the MS vector was sustainable for almost 3 months. Our data suggested that the MS vector would be suitable to construct mice containing the transgene expressed in the brain or spinal cord in a quick and cost-effective manner. More... »

PAGES

396-403

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/gt.2011.120

DOI

http://dx.doi.org/10.1038/gt.2011.120

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016179464

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21900964


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Brain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luciferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Moloney murine leukemia virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neural Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Retroviridae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spleen Focus-Forming Viruses", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Terminal Repeat Sequences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ultrasonography", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Interdisciplinary Graduate Program in Genetic Engineering, Seoul National University, Seoul, Korea", 
          "id": "http://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "Interdisciplinary Graduate Program in Genetic Engineering, Seoul National University, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jang", 
        "givenName": "J", 
        "id": "sg:person.01102152417.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102152417.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Life Science and Biotechnology, Sungkyunkwan University, Seoul, Korea", 
          "id": "http://www.grid.ac/institutes/grid.264381.a", 
          "name": [
            "School of Life Science and Biotechnology, Sungkyunkwan University, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoon", 
        "givenName": "K", 
        "id": "sg:person.01221514013.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221514013.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea", 
          "id": "http://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hwang", 
        "givenName": "D W", 
        "id": "sg:person.0627125000.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627125000.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "WCU Department of Molecular Medicine and Biopharmaceutical Science, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea", 
          "id": "http://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "WCU Department of Molecular Medicine and Biopharmaceutical Science, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "D S", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Biological Sciences, Seoul National University, Seoul, Korea", 
          "id": "http://www.grid.ac/institutes/grid.31501.36", 
          "name": [
            "Interdisciplinary Graduate Program in Genetic Engineering, Seoul National University, Seoul, Korea", 
            "School of Biological Sciences, Seoul National University, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "S", 
        "id": "sg:person.01025624231.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025624231.33"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/298623a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026714891", 
          "https://doi.org/10.1038/298623a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301892", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049235504", 
          "https://doi.org/10.1038/sj.gt.3301892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/12186", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027048246", 
          "https://doi.org/10.1038/12186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/301032a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039587163", 
          "https://doi.org/10.1038/301032a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3303010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051273018", 
          "https://doi.org/10.1038/sj.gt.3303010"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-09-08", 
    "datePublishedReg": "2011-09-08", 
    "description": "Gene transfer to the early-stage embryonic brain using the ultrasound image-guided gene delivery (UIGD) technique has proven to be valuable for investigating brain development. Thus far, this technology has been restricted to the study of embryonic neurogenesis. When this technique is designed to be employed for the study in adult animals, a long-term stable gene expression will be required. We attempted to develop a retroviral vector suitable for expressing exogenous genes in the brains of postnatal and adult mice in the context of the UIGD technique. Retroviral vectors containing four different long terminal repeats (LTRs) (each from Moloney murine leukemia virus (MoMLV), murine stem cell virus (MSCV), myeloproliferative sarcoma virus (MPSV) and spleen focus-forming virus (SFFV)) were compared using the well-known CE vector having the EF1\u03b1 internal promoter as a control. The MS vector containing MSCV LTR produced a higher viral titer and a higher level of gene expression than other vectors including CE. The MS vector drove the gene expression in cultured neural stem cells for 3 weeks. Furthermore, the MS vector could efficiently deliver the gene to the mouse central nervous system, as transgene expression was found in various regions of the brains and spinal cords as well as in all major neural cell types. The data from an in vivo luciferase imaging analysis showed that the gene expression from the MS vector was sustainable for almost 3 months. Our data suggested that the MS vector would be suitable to construct mice containing the transgene expressed in the brain or spinal cord in a quick and cost-effective manner.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/gt.2011.120", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1105638", 
        "issn": [
          "0969-7128", 
          "1476-5462"
        ], 
        "name": "Gene Therapy", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "keywords": [
      "retroviral vectors", 
      "MSCV long terminal repeat", 
      "gene delivery techniques", 
      "different long terminal repeats", 
      "stable gene expression", 
      "MS vector", 
      "long-term stable gene expression", 
      "gene delivery", 
      "brain of postnatal", 
      "transgene expression", 
      "exogenous genes", 
      "major neural cell types", 
      "spinal cord", 
      "gene transfer", 
      "delivery techniques", 
      "cost-effective manner", 
      "mouse central nervous system", 
      "cultured neural stem cells", 
      "long terminal repeat", 
      "central nervous system", 
      "high viral titers", 
      "stem cells", 
      "neural stem cells", 
      "gene expression", 
      "neural cell types", 
      "adult mice", 
      "nervous system", 
      "viral titers", 
      "mouse brain", 
      "brain development", 
      "embryonic brain", 
      "brain", 
      "adult animals", 
      "embryonic neurogenesis", 
      "vector", 
      "internal promoter", 
      "cord", 
      "mice", 
      "transgene", 
      "cell types", 
      "technique", 
      "expression", 
      "imaging analysis", 
      "technology", 
      "delivery", 
      "high levels", 
      "terminal repeat", 
      "postnatal", 
      "neurogenesis", 
      "titers", 
      "weeks", 
      "months", 
      "M vector", 
      "study", 
      "genes", 
      "Ce", 
      "animals", 
      "cells", 
      "transfer", 
      "levels", 
      "data", 
      "control", 
      "system", 
      "promoter", 
      "manner", 
      "development", 
      "types", 
      "analysis", 
      "repeats", 
      "region", 
      "context"
    ], 
    "name": "A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain", 
    "pagination": "396-403", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1016179464"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/gt.2011.120"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21900964"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/gt.2011.120", 
      "https://app.dimensions.ai/details/publication/pub.1016179464"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:37", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_553.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/gt.2011.120"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/gt.2011.120'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/gt.2011.120'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/gt.2011.120'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/gt.2011.120'


 

This table displays all metadata directly associated to this object as RDF triples.

252 TRIPLES      21 PREDICATES      117 URIs      104 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/gt.2011.120 schema:about N09daa0f404fe44ab82c07039407a8f6a
2 N0ae3bbb425784e129e40917d38d338ba
3 N110e66c9283d44288c9f8b833c6bd77b
4 N13852b1bacb24c699410ab8208c3196f
5 N1a68340633e0432b8b38b04f381a124f
6 N383b2d3ff4cc4b84b2765836e372315d
7 N4283cf307c0d44e9b25f9818096c6b0b
8 N4c0017eb5b2d48e68ed788a99ca3cce5
9 N568f27442bc5437fae919724f8d9209a
10 Na0425dec5e5344969838954aa2c1c2f5
11 Na3936d86e4af4b61bfabe04d14c0aa25
12 Nbd0d6de9ba704d34b8cd4d3f13209d63
13 Nbf9c492fa79e4f6fb8735eaa3e20fd66
14 Nc7d4f8ad422444f0ade1129e0f537faa
15 Ne08ce762d7354a7caef51ce272be3ebb
16 Ne6e3e35749684c5bae63c97c03ffedeb
17 anzsrc-for:11
18 anzsrc-for:1109
19 schema:author N04531e20659140cc841ede03ebfdbe49
20 schema:citation sg:pub.10.1038/12186
21 sg:pub.10.1038/298623a0
22 sg:pub.10.1038/301032a0
23 sg:pub.10.1038/sj.gt.3301892
24 sg:pub.10.1038/sj.gt.3303010
25 schema:datePublished 2011-09-08
26 schema:datePublishedReg 2011-09-08
27 schema:description Gene transfer to the early-stage embryonic brain using the ultrasound image-guided gene delivery (UIGD) technique has proven to be valuable for investigating brain development. Thus far, this technology has been restricted to the study of embryonic neurogenesis. When this technique is designed to be employed for the study in adult animals, a long-term stable gene expression will be required. We attempted to develop a retroviral vector suitable for expressing exogenous genes in the brains of postnatal and adult mice in the context of the UIGD technique. Retroviral vectors containing four different long terminal repeats (LTRs) (each from Moloney murine leukemia virus (MoMLV), murine stem cell virus (MSCV), myeloproliferative sarcoma virus (MPSV) and spleen focus-forming virus (SFFV)) were compared using the well-known CE vector having the EF1α internal promoter as a control. The MS vector containing MSCV LTR produced a higher viral titer and a higher level of gene expression than other vectors including CE. The MS vector drove the gene expression in cultured neural stem cells for 3 weeks. Furthermore, the MS vector could efficiently deliver the gene to the mouse central nervous system, as transgene expression was found in various regions of the brains and spinal cords as well as in all major neural cell types. The data from an in vivo luciferase imaging analysis showed that the gene expression from the MS vector was sustainable for almost 3 months. Our data suggested that the MS vector would be suitable to construct mice containing the transgene expressed in the brain or spinal cord in a quick and cost-effective manner.
28 schema:genre article
29 schema:isAccessibleForFree false
30 schema:isPartOf N2a4ad36ca6094874b70c31f3f0f53f52
31 N5e18e273e0cf458ab63a6be65c8f219c
32 sg:journal.1105638
33 schema:keywords Ce
34 M vector
35 MS vector
36 MSCV long terminal repeat
37 adult animals
38 adult mice
39 analysis
40 animals
41 brain
42 brain development
43 brain of postnatal
44 cell types
45 cells
46 central nervous system
47 context
48 control
49 cord
50 cost-effective manner
51 cultured neural stem cells
52 data
53 delivery
54 delivery techniques
55 development
56 different long terminal repeats
57 embryonic brain
58 embryonic neurogenesis
59 exogenous genes
60 expression
61 gene delivery
62 gene delivery techniques
63 gene expression
64 gene transfer
65 genes
66 high levels
67 high viral titers
68 imaging analysis
69 internal promoter
70 levels
71 long terminal repeat
72 long-term stable gene expression
73 major neural cell types
74 manner
75 mice
76 months
77 mouse brain
78 mouse central nervous system
79 nervous system
80 neural cell types
81 neural stem cells
82 neurogenesis
83 postnatal
84 promoter
85 region
86 repeats
87 retroviral vectors
88 spinal cord
89 stable gene expression
90 stem cells
91 study
92 system
93 technique
94 technology
95 terminal repeat
96 titers
97 transfer
98 transgene
99 transgene expression
100 types
101 vector
102 viral titers
103 weeks
104 schema:name A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain
105 schema:pagination 396-403
106 schema:productId N1e0873fdb62b4c579a7212ae61ee6e9c
107 N25b3d891a76a4eea9d754e067f9c0508
108 N692ccd8719cb44f6af51b43d6b226eac
109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016179464
110 https://doi.org/10.1038/gt.2011.120
111 schema:sdDatePublished 2022-10-01T06:37
112 schema:sdLicense https://scigraph.springernature.com/explorer/license/
113 schema:sdPublisher N4edd710b7d1d47daa542749e845c0b55
114 schema:url https://doi.org/10.1038/gt.2011.120
115 sgo:license sg:explorer/license/
116 sgo:sdDataset articles
117 rdf:type schema:ScholarlyArticle
118 N04531e20659140cc841ede03ebfdbe49 rdf:first sg:person.01102152417.00
119 rdf:rest Nc84bc3695cc34e1cbba986cff0511ead
120 N09daa0f404fe44ab82c07039407a8f6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Spleen Focus-Forming Viruses
122 rdf:type schema:DefinedTerm
123 N0ae3bbb425784e129e40917d38d338ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Genetic Vectors
125 rdf:type schema:DefinedTerm
126 N110e66c9283d44288c9f8b833c6bd77b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Moloney murine leukemia virus
128 rdf:type schema:DefinedTerm
129 N13852b1bacb24c699410ab8208c3196f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Luciferases
131 rdf:type schema:DefinedTerm
132 N1a68340633e0432b8b38b04f381a124f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Cell Line
134 rdf:type schema:DefinedTerm
135 N1e0873fdb62b4c579a7212ae61ee6e9c schema:name pubmed_id
136 schema:value 21900964
137 rdf:type schema:PropertyValue
138 N25b3d891a76a4eea9d754e067f9c0508 schema:name doi
139 schema:value 10.1038/gt.2011.120
140 rdf:type schema:PropertyValue
141 N2a4ad36ca6094874b70c31f3f0f53f52 schema:issueNumber 4
142 rdf:type schema:PublicationIssue
143 N383b2d3ff4cc4b84b2765836e372315d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Neural Stem Cells
145 rdf:type schema:DefinedTerm
146 N4283cf307c0d44e9b25f9818096c6b0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Male
148 rdf:type schema:DefinedTerm
149 N44d2fcea000e4c63b77ae1f230d96112 rdf:first sg:person.01025624231.33
150 rdf:rest rdf:nil
151 N4c0017eb5b2d48e68ed788a99ca3cce5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Gene Expression
153 rdf:type schema:DefinedTerm
154 N4edd710b7d1d47daa542749e845c0b55 schema:name Springer Nature - SN SciGraph project
155 rdf:type schema:Organization
156 N568f27442bc5437fae919724f8d9209a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Terminal Repeat Sequences
158 rdf:type schema:DefinedTerm
159 N5bc45abe27704b62b832f9edafe9c03b schema:affiliation grid-institutes:grid.31501.36
160 schema:familyName Lee
161 schema:givenName D S
162 rdf:type schema:Person
163 N5e18e273e0cf458ab63a6be65c8f219c schema:volumeNumber 19
164 rdf:type schema:PublicationVolume
165 N692ccd8719cb44f6af51b43d6b226eac schema:name dimensions_id
166 schema:value pub.1016179464
167 rdf:type schema:PropertyValue
168 N84e406eefead48f89ab508365b898219 rdf:first sg:person.0627125000.90
169 rdf:rest N94903dd7994c438c80b4e2cd06b9dc75
170 N94903dd7994c438c80b4e2cd06b9dc75 rdf:first N5bc45abe27704b62b832f9edafe9c03b
171 rdf:rest N44d2fcea000e4c63b77ae1f230d96112
172 Na0425dec5e5344969838954aa2c1c2f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Animals
174 rdf:type schema:DefinedTerm
175 Na3936d86e4af4b61bfabe04d14c0aa25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Gene Transfer Techniques
177 rdf:type schema:DefinedTerm
178 Nbd0d6de9ba704d34b8cd4d3f13209d63 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Ultrasonography
180 rdf:type schema:DefinedTerm
181 Nbf9c492fa79e4f6fb8735eaa3e20fd66 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Mice
183 rdf:type schema:DefinedTerm
184 Nc7d4f8ad422444f0ade1129e0f537faa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Retroviridae
186 rdf:type schema:DefinedTerm
187 Nc84bc3695cc34e1cbba986cff0511ead rdf:first sg:person.01221514013.23
188 rdf:rest N84e406eefead48f89ab508365b898219
189 Ne08ce762d7354a7caef51ce272be3ebb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
190 schema:name Brain
191 rdf:type schema:DefinedTerm
192 Ne6e3e35749684c5bae63c97c03ffedeb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Genes, Reporter
194 rdf:type schema:DefinedTerm
195 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
196 schema:name Medical and Health Sciences
197 rdf:type schema:DefinedTerm
198 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
199 schema:name Neurosciences
200 rdf:type schema:DefinedTerm
201 sg:journal.1105638 schema:issn 0969-7128
202 1476-5462
203 schema:name Gene Therapy
204 schema:publisher Springer Nature
205 rdf:type schema:Periodical
206 sg:person.01025624231.33 schema:affiliation grid-institutes:grid.31501.36
207 schema:familyName Kim
208 schema:givenName S
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025624231.33
210 rdf:type schema:Person
211 sg:person.01102152417.00 schema:affiliation grid-institutes:grid.31501.36
212 schema:familyName Jang
213 schema:givenName J
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102152417.00
215 rdf:type schema:Person
216 sg:person.01221514013.23 schema:affiliation grid-institutes:grid.264381.a
217 schema:familyName Yoon
218 schema:givenName K
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221514013.23
220 rdf:type schema:Person
221 sg:person.0627125000.90 schema:affiliation grid-institutes:grid.31501.36
222 schema:familyName Hwang
223 schema:givenName D W
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627125000.90
225 rdf:type schema:Person
226 sg:pub.10.1038/12186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027048246
227 https://doi.org/10.1038/12186
228 rdf:type schema:CreativeWork
229 sg:pub.10.1038/298623a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026714891
230 https://doi.org/10.1038/298623a0
231 rdf:type schema:CreativeWork
232 sg:pub.10.1038/301032a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039587163
233 https://doi.org/10.1038/301032a0
234 rdf:type schema:CreativeWork
235 sg:pub.10.1038/sj.gt.3301892 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049235504
236 https://doi.org/10.1038/sj.gt.3301892
237 rdf:type schema:CreativeWork
238 sg:pub.10.1038/sj.gt.3303010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051273018
239 https://doi.org/10.1038/sj.gt.3303010
240 rdf:type schema:CreativeWork
241 grid-institutes:grid.264381.a schema:alternateName School of Life Science and Biotechnology, Sungkyunkwan University, Seoul, Korea
242 schema:name School of Life Science and Biotechnology, Sungkyunkwan University, Seoul, Korea
243 rdf:type schema:Organization
244 grid-institutes:grid.31501.36 schema:alternateName Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea
245 Interdisciplinary Graduate Program in Genetic Engineering, Seoul National University, Seoul, Korea
246 School of Biological Sciences, Seoul National University, Seoul, Korea
247 WCU Department of Molecular Medicine and Biopharmaceutical Science, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea
248 schema:name Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea
249 Interdisciplinary Graduate Program in Genetic Engineering, Seoul National University, Seoul, Korea
250 School of Biological Sciences, Seoul National University, Seoul, Korea
251 WCU Department of Molecular Medicine and Biopharmaceutical Science, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea
252 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...