Ontology type: schema:ScholarlyArticle Open Access: True
2011-03
AUTHORSAnnie Wu, Qi Zeng, Tae Heung Kang, Shiwen Peng, Elena Roosinovich, Sara I. Pai, Chien-Fu Hung
ABSTRACTHuman papillomavirus (HPV), particularly type 16, has been associated with a subset of head and neck cancers. The viral-encoded oncogenic proteins E6 and E7 represent ideal targets for immunotherapy against HPV-associated head and neck cancers. DNA vaccines have emerged as attractive approaches for immunotherapy due to its simplicity, safety and ease of preparation. Intradermal administration of DNA vaccine by means of gene gun represents an efficient method to deliver DNA directly into dendritic cells for priming antigen-specific T cells. We have previously shown that a DNA vaccine encoding an invariant chain (Ii), in which the class II-associated Ii peptide (CLIP) region has been replaced by a Pan-DR-epitope (PADRE) sequence to form Ii-PADRE, is capable of generating PADRE-specific CD4+ T cells in vaccinated mice. In the current study, we hypothesize that a DNA vaccine encoding Ii-PADRE linked to E6 (Ii-PADRE-E6) will further enhance E6-specific CD8+ T cell immune responses through PADRE-specific CD4+ T-helper cells. We found that mice vaccinated with Ii-PADRE-E6 DNA generated comparable levels of PADRE-specific CD4+ T-cell immune responses, as well as significantly stronger E6-specific CD8+ T-cell immune responses and antitumor effects against the lethal challenge of E6-expressing tumor compared with mice vaccinated with Ii-E6 DNA. Taken together, our data indicate that vaccination with Ii-E6 DNA with PADRE replacing the CLIP region is capable of enhancing the E6-specific CD8+ T-cell immune response generated by the Ii-E6 DNA. Thus, Ii-PADRE-E6 represents a novel DNA vaccine for the treatment of HPV-associated head and neck cancer and other HPV-associated malignancies. More... »
PAGES304
http://scigraph.springernature.com/pub.10.1038/gt.2010.151
DOIhttp://dx.doi.org/10.1038/gt.2010.151
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1015101412
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/20981112
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Immunology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antigens, Differentiation, B-Lymphocyte",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biolistics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "CD4-Positive T-Lymphocytes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "CD8-Positive T-Lymphocytes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line, Tumor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA Primers",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Dendritic Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Epitopes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Flow Cytometry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Head and Neck Neoplasms",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Histocompatibility Antigens Class II",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Human papillomavirus 16",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Immunotherapy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Inbred C57BL",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Oncogene Proteins, Viral",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Papillomavirus Infections",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Repressor Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Reverse Transcriptase Polymerase Chain Reaction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Vaccines, DNA",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Johns Hopkins University",
"id": "https://www.grid.ac/institutes/grid.21107.35",
"name": [
"Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD",
"Department of Otolaryngology - Head and Neck Cancer, Johns Hopkins Medical Institutions, Baltimore, MD"
],
"type": "Organization"
},
"familyName": "Wu",
"givenName": "Annie",
"id": "sg:person.07764333747.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07764333747.54"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Johns Hopkins University",
"id": "https://www.grid.ac/institutes/grid.21107.35",
"name": [
"Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD"
],
"type": "Organization"
},
"familyName": "Zeng",
"givenName": "Qi",
"id": "sg:person.01057651641.91",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057651641.91"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Johns Hopkins University",
"id": "https://www.grid.ac/institutes/grid.21107.35",
"name": [
"Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD"
],
"type": "Organization"
},
"familyName": "Kang",
"givenName": "Tae Heung",
"id": "sg:person.0663641732.90",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663641732.90"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Johns Hopkins University",
"id": "https://www.grid.ac/institutes/grid.21107.35",
"name": [
"Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD"
],
"type": "Organization"
},
"familyName": "Peng",
"givenName": "Shiwen",
"id": "sg:person.0637425714.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637425714.18"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Imperial College London",
"id": "https://www.grid.ac/institutes/grid.7445.2",
"name": [
"Imperial College School of Medicine, London, UK"
],
"type": "Organization"
},
"familyName": "Roosinovich",
"givenName": "Elena",
"id": "sg:person.01031133326.15",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031133326.15"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Johns Hopkins University",
"id": "https://www.grid.ac/institutes/grid.21107.35",
"name": [
"Department of Otolaryngology - Head and Neck Cancer, Johns Hopkins Medical Institutions, Baltimore, MD",
"Department of Oncology, Johns Hopkins Medical Institutions, Baltimore, MD"
],
"type": "Organization"
},
"familyName": "Pai",
"givenName": "Sara I.",
"id": "sg:person.01053524376.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053524376.38"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Johns Hopkins University",
"id": "https://www.grid.ac/institutes/grid.21107.35",
"name": [
"Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD",
"Department of Oncology, Johns Hopkins Medical Institutions, Baltimore, MD"
],
"type": "Organization"
},
"familyName": "Hung",
"givenName": "Chien-Fu",
"id": "sg:person.014470015607.97",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014470015607.97"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1146/annurev.immunol.15.1.617",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000338683"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3303102",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002210451",
"https://doi.org/10.1038/sj.gt.3303102"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1084/jem.188.6.1075",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003271274"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4049/jimmunol.164.3.1625",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004870504"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1586/14760584.6.2.227",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009808395"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.imlet.2007.09.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015705089"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302913",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017178463",
"https://doi.org/10.1038/sj.gt.3302913"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11373-008-9247-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021923824",
"https://doi.org/10.1007/s11373-008-9247-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302252",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024719788",
"https://doi.org/10.1038/sj.gt.3302252"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302252",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024719788",
"https://doi.org/10.1038/sj.gt.3302252"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/1078-0432.ccr-09-0784",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026807381"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/0008-5472.can-03-1475",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027792944"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc798",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030753299",
"https://doi.org/10.1038/nrc798"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc798",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030753299",
"https://doi.org/10.1038/nrc798"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1038/sj.mt.6300121",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038083198"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.vaccine.2007.08.036",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039233604"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.cco.0000264952.98166.99",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040568195"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.cco.0000264952.98166.99",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040568195"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.immunol.18.1.927",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043301612"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302840",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044443511",
"https://doi.org/10.1038/sj.gt.3302840"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302840",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044443511",
"https://doi.org/10.1038/sj.gt.3302840"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.78.16.8468-8476.2004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051051776"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nm1096-1122",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052270183",
"https://doi.org/10.1038/nm1096-1122"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1074-7613(94)80017-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053296492"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0264-410x(98)00341-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053433455"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/vir.0.002105-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060398099"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci12346",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1063416061"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci17293",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1063416263"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1517/14712598.8.4.421",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1067590027"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074601062",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074736041",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1075261856",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4049/jimmunol.180.5.3339",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077610835"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082854625",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1083056569",
"type": "CreativeWork"
}
],
"datePublished": "2011-03",
"datePublishedReg": "2011-03-01",
"description": "Human papillomavirus (HPV), particularly type 16, has been associated with a subset of head and neck cancers. The viral-encoded oncogenic proteins E6 and E7 represent ideal targets for immunotherapy against HPV-associated head and neck cancers. DNA vaccines have emerged as attractive approaches for immunotherapy due to its simplicity, safety and ease of preparation. Intradermal administration of DNA vaccine by means of gene gun represents an efficient method to deliver DNA directly into dendritic cells for priming antigen-specific T cells. We have previously shown that a DNA vaccine encoding an invariant chain (Ii), in which the class II-associated Ii peptide (CLIP) region has been replaced by a Pan-DR-epitope (PADRE) sequence to form Ii-PADRE, is capable of generating PADRE-specific CD4+ T cells in vaccinated mice. In the current study, we hypothesize that a DNA vaccine encoding Ii-PADRE linked to E6 (Ii-PADRE-E6) will further enhance E6-specific CD8+ T cell immune responses through PADRE-specific CD4+ T-helper cells. We found that mice vaccinated with Ii-PADRE-E6 DNA generated comparable levels of PADRE-specific CD4+ T-cell immune responses, as well as significantly stronger E6-specific CD8+ T-cell immune responses and antitumor effects against the lethal challenge of E6-expressing tumor compared with mice vaccinated with Ii-E6 DNA. Taken together, our data indicate that vaccination with Ii-E6 DNA with PADRE replacing the CLIP region is capable of enhancing the E6-specific CD8+ T-cell immune response generated by the Ii-E6 DNA. Thus, Ii-PADRE-E6 represents a novel DNA vaccine for the treatment of HPV-associated head and neck cancer and other HPV-associated malignancies.",
"genre": "research_article",
"id": "sg:pub.10.1038/gt.2010.151",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.2478134",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2440195",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2440185",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1105638",
"issn": [
"0969-7128",
"1476-5462"
],
"name": "Gene Therapy",
"type": "Periodical"
},
{
"issueNumber": "3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "18"
}
],
"name": "Innovative DNA vaccine for human papillomavirus (HPV)-associated head and neck cancer",
"pagination": "304",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"47f2c8efd63fa246983225c834856fcae06e20bcf622c640c2b26ae188330021"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"20981112"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9421525"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/gt.2010.151"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1015101412"
]
}
],
"sameAs": [
"https://doi.org/10.1038/gt.2010.151",
"https://app.dimensions.ai/details/publication/pub.1015101412"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T22:20",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000435.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/gt2010151"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/gt.2010.151'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/gt.2010.151'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/gt.2010.151'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/gt.2010.151'
This table displays all metadata directly associated to this object as RDF triples.
305 TRIPLES
21 PREDICATES
82 URIs
43 LITERALS
31 BLANK NODES