Noncarrier naked antigen-specific DNA vaccine generates potent antigen-specific immunologic responses and antitumor effects View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-06

AUTHORS

C-A Chen, M-C Chang, W-Z Sun, Y-L Chen, Y-C Chiang, C-Y Hsieh, S M Chen, P-N Hsiao, W-F Cheng

ABSTRACT

Genetic immunization strategies have largely focused on the use of plasmid DNA with a gene gun. However, there remains a clear need to further improve the efficiency, safety, and cost of potential DNA vaccines. The gold particle-coated DNA format delivered through a gene gun is expensive, time and process consuming, and raises aseptic safety concerns. This study aims to determine whether a low-pressured gene gun can deliver noncarrier naked DNA vaccine without any particle coating, and generate similarly strong antigen-specific immunologic responses and potent antitumor effects compared with gold particle-coated DNA vaccine. Our results show that mice vaccinated with noncarrier naked chimeric CRT/E7 DNA lead to dramatic increases in the numbers of E7-specific CD8+ T-cell precursors and markedly raised titers of E7-specific antibodies. Furthermore, noncarrier naked CRT/E7 DNA vaccine generated potent antitumor effects against subcutaneous E7-expressing tumors and pre-established E7-expressing metastatic pulmonary tumors. In addition, mice immunized with noncarrier naked CRT/E7 DNA vaccine had significantly less burning effects on the skin compared with those vaccinated with gold particle-coated CRT/E7 DNA vaccine. We conclude that noncarrier naked CRT/E7 DNA vaccine delivered with a low-pressured gene gun can generate similarly potent immunologic responses and effective antitumor effects has fewer side effects, and is more convenient than conventional gold particle-coated DNA vaccine. More... »

PAGES

gt200931

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/gt.2009.31

DOI

http://dx.doi.org/10.1038/gt.2009.31

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036322495

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19357714


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