The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-12

AUTHORS

P O Vardevanyan, A P Antonyan, M A Parsadanyan, H G Davtyan, A T Karapetyan

ABSTRACT

The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25(o)C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of bases (n), corresponding to one binding site were determined. Non fluorescent "strong" complex with ds-DNA at pH = 7.0 and t = 25(o)C as well as "strong" and "weak" complexes with ss-DNA at pH = 3.0 and t = 25(o)C are revealed. More... »

PAGES

emm200368

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/emm.2003.68

DOI

http://dx.doi.org/10.1038/emm.2003.68

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032609270

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14749530


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