Study of ethidium bromide interaction peculiarities with DNA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2001-12

AUTHORS

P O Vardevanyan, A P Antonyan, G A Manukyan, A T Karapetyan

ABSTRACT

The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M < or = muNa+ < or = 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex--(deltaT) becomes equal to that of DNA itself (deltaTo). It has been shown that the values of r(b) at which delta deltaT=deltaT-deltaT0=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA. More... »

PAGES

205

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/emm.2001.34

DOI

http://dx.doi.org/10.1038/emm.2001.34

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009175078

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11795481


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