New insights into the phenotype of human dendritic cell populations View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-01-29

AUTHORS

Georgina Jane Clark, Fiona Kupresanin, Phillip Dieter Fromm, Xinsheng Ju, Leticia Muusers, Pablo Alejandro Silveira, Zehra Elgundi, Robin Edward Gasiorowski, Michael Stefan Papadimitrious, Christian Bryant, Kenneth Maan Liong Lee, Candice Jane Clarke, James William Young, Andrew Chan, Andrew Harman, Rachel Botting, Raquel Cabezón, Daniel Benitez-Ribas, Anna Elizabeth Stella Brooks, Phillip Rodney Dunbar, Derek Nigel John Hart

ABSTRACT

HLDA10 is the Tenth Human Leukocyte Differentiation Antigen (HLDA) Workshop. The HLDA Workshops provide a mechanism to allocate cluster of differentiation (CD) nomenclature by engaging in interlaboratory studies. As the host laboratory, we invited researchers from national and international academic and commercial institutions to submit monoclonal antibodies (mAbs) to human leukocyte surface membrane molecules, particularly those that recognised molecules on human myeloid cell populations and dendritic cells (DCs). These mAbs were tested for activity and then distributed as a blinded panel to 15 international laboratories to test on different leukocyte populations. These populations included blood DCs, skin-derived DCs, tonsil leukocytes, monocyte-derived DCs, CD34-derived DCs, macrophage populations and diagnostic acute myeloid leukaemia and lymphoma samples. Each laboratory was provided with enough mAb to perform five repeat experiments. Here, we summarise the reactivity of different mAb to 68 different cell-surface molecules expressed by human myeloid and DC populations. Submitted mAbs to some of the molecules were further validated to collate data required to designate a formal CD number. This collaborative process provides the broader scientific community with an invaluable data set validating mAbs to leukocyte-surface molecules. More... »

PAGES

e61

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/cti.2015.40

DOI

http://dx.doi.org/10.1038/cti.2015.40

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021224454

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26900474


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