Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-06

AUTHORS

Jiekai Chen, Jing Liu, You Chen, Jiaqi Yang, Jing Chen, He Liu, Xiangjie Zhao, Kunlun Mo, Hong Song, Lin Guo, Shilong Chu, Deping Wang, Ke Ding, Duanqing Pei

ABSTRACT

The ectopic expression of several transcription factors can restore embryonic cell fate to cultured somatic cells and generate induced pluripotent stem cells (iPSCs), revealing a previously unknown pathway to pluripotency. However, this technology is currently limited by low efficiency, slow kinetics and multi-factorial requirement. Here we show that reprogramming can be improved and dramatically accelerated by optimizing culture conditions. First, we developed an optimized defined medium, iCD1, which allows Oct4/Sox2/Klf4 (OSK)-mediated reprogramming to achieve ultra-high efficiency (~10% at day 8). We also found that this optimized condition renders both Sox2 and Klf4 dispensable, although the elimination of these two factors leads to lower efficiency and slower kinetics. Our studies define a shortened route, both in timing and factor requirement, toward pluripotency. This new paradigm not only provides a rationale to further improve iPSC generation but also simplifies the conceptual understanding of reprogramming by defined factors. More... »

PAGES

884

Journal

TITLE

Cell Research

ISSUE

6

VOLUME

21

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/cr.2011.51

DOI

http://dx.doi.org/10.1038/cr.2011.51

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049791045

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21445094


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