Monomeric type I and type III transforming growth factor-β receptors and their dimerization revealed by single-molecule imaging View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-11

AUTHORS

Wei Zhang, Jinghe Yuan, Yong Yang, Li Xu, Qiang Wang, Wei Zuo, Xiaohong Fang, Ye-Guang Chen

ABSTRACT

Transforming growth factor-β (TGF-β) binds with two transmembrane serine/threonine kinase receptors, type II (TβRII) and type I receptors (TβRI), and one accessory receptor, type III receptor (TβRIII), to transduce signals across cell membranes. Previous biochemical studies suggested that TβRI and TβRIII are preexisted homo-dimers. Using single-molecule microscopy to image green fluorescent protein-labeled membrane proteins, for the first time we have demonstrated that TβRI and TβRIII could exist as monomers at a low expression level. Upon TGF-β1 stimulation, TβRI follows the general ligand-induced receptor dimerization model for activation, but this process is TβRII-dependent. The monomeric status of the non-kinase receptor TβRIII is unchanged in the presence of TGF-β1. With the increase of receptor expression, both TβRI and TβRIII can be assembled into dimers on cell surfaces. More... »

PAGES

1216

References to SciGraph publications

  • 2007-04. Subunit counting in membrane-bound proteins in NATURE METHODS
  • 2008-01. Subunit organization and functional transitions in Ci-VSP in NATURE STRUCTURAL & MOLECULAR BIOLOGY
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    http://scigraph.springernature.com/pub.10.1038/cr.2010.105

    DOI

    http://dx.doi.org/10.1038/cr.2010.105

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20625381


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