Deletion of the E3-6.7K/gp19K region reduces the persistence of wild-type adenovirus in a permissive tumor model in Syrian hamsters View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-09

AUTHORS

S Bortolanza, M Bunuales, P Alzuguren, O Lamas, R Aldabe, J Prieto, R Hernandez-Alcoceba

ABSTRACT

A partial deletion of the adenovirus E3 region, comprising the overlapping 6.7K/gp19K genes, has been described for the incorporation of therapeutic genes in 'armed' oncolytic adenoviruses. This deletion allows the insertion of up to 2.5 kb genetic material into the virus and ensures strong expression of transgenes without reducing the replication and cytolytic potency of viruses in vitro. E3-gp19K and 6.7K proteins are involved in avoiding recognition and elimination of infected cells by the host immune system. Therefore, we have studied the effect of this deletion on the replication and transgene expression of the virus in immunocompetent models based on Syrian hamsters. Tumors were established by intrahepatic injection of pancreatic cancer cells with moderate (HaP-T1, HP-1) or low (H2T) permissivity for adenovirus replication. The wild-type human adenovirus 5 (Ad5) or a modified version containing the luciferase gene in the E3-6.7K/gp19K locus (Ad-WTLuc) were injected intratumorally. We found that elimination of Ad-WTLuc was faster than Ad5 in HaP-T1 and HP-1 tumors. In contrast, no differences were observed when the same tumor was established in severely immunocompromised NOD-scid IL2Rgamma(null) mice. In addition, virus-mediated luciferase expression was more stable in these animals. These results suggest that the lack of E3-6.7K/gp19K genes may accelerate the clearance of oncolytic adenoviruses in some immunocompetent tumor models. More... »

PAGES

cgt200912

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/cgt.2009.12

DOI

http://dx.doi.org/10.1038/cgt.2009.12

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038981909

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19229289


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenoviridae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenovirus E3 Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetinae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Deletion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genome, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunocompetence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesocricetus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oncolytic Virotherapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Xenograft Model Antitumor Assays", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Navarra", 
          "id": "https://www.grid.ac/institutes/grid.5924.a", 
          "name": [
            "Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bortolanza", 
        "givenName": "S", 
        "id": "sg:person.0603175126.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603175126.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Navarra", 
          "id": "https://www.grid.ac/institutes/grid.5924.a", 
          "name": [
            "Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bunuales", 
        "givenName": "M", 
        "id": "sg:person.0717423526.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717423526.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Navarra", 
          "id": "https://www.grid.ac/institutes/grid.5924.a", 
          "name": [
            "Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alzuguren", 
        "givenName": "P", 
        "id": "sg:person.01065406061.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065406061.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Navarra", 
          "id": "https://www.grid.ac/institutes/grid.5924.a", 
          "name": [
            "Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lamas", 
        "givenName": "O", 
        "id": "sg:person.01034562125.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034562125.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Navarra", 
          "id": "https://www.grid.ac/institutes/grid.5924.a", 
          "name": [
            "Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aldabe", 
        "givenName": "R", 
        "id": "sg:person.01142515214.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142515214.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto de Salud Carlos III", 
          "id": "https://www.grid.ac/institutes/grid.413448.e", 
          "name": [
            "Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain", 
            "CIBERehd, University Clinic, Navarra, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Prieto", 
        "givenName": "J", 
        "id": "sg:person.0770060660.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770060660.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Navarra", 
          "id": "https://www.grid.ac/institutes/grid.5924.a", 
          "name": [
            "Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hernandez-Alcoceba", 
        "givenName": "R", 
        "id": "sg:person.01017272661.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01017272661.71"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01487691", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000233423", 
          "https://doi.org/10.1007/bf01487691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.76.4.1578-1587.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000666510"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301508", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007414000", 
          "https://doi.org/10.1038/sj.gt.3301508"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301508", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007414000", 
          "https://doi.org/10.1038/sj.gt.3301508"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/mt.2008.162", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007700261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/mt.2008.162", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007700261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-79496-4_13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007732172", 
          "https://doi.org/10.1007/978-3-642-79496-4_13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cgt.7700542", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008482065", 
          "https://doi.org/10.1038/sj.cgt.7700542"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cgt.7700542", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008482065", 
          "https://doi.org/10.1038/sj.cgt.7700542"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.174.10.6477", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010296345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301509", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010537663", 
          "https://doi.org/10.1038/sj.gt.3301509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301509", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010537663", 
          "https://doi.org/10.1038/sj.gt.3301509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m008218200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011814898"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-05-3497", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021463586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-540-75647-7_3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021537765", 
          "https://doi.org/10.1007/978-3-540-75647-7_3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1525-0016(03)00199-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022590672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1525-0016(03)00199-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022590672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.78.22.12297-12307.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022723576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1209037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025367553", 
          "https://doi.org/10.1038/sj.onc.1209037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1209037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025367553", 
          "https://doi.org/10.1038/sj.onc.1209037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1209037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025367553", 
          "https://doi.org/10.1038/sj.onc.1209037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetpar.2006.04.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027608718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.5.1356", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029403876"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jgm.495", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036710781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt887", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043212288", 
          "https://doi.org/10.1038/nbt887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt887", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043212288", 
          "https://doi.org/10.1038/nbt887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301507", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049636162", 
          "https://doi.org/10.1038/sj.gt.3301507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301507", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049636162", 
          "https://doi.org/10.1038/sj.gt.3301507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.79.9.5455-5465.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050164659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.77.14.7764-7778.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050881287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/104303402760293574", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059202491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.2007.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059272996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02924367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075325265", 
          "https://doi.org/10.1007/bf02924367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082778756", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082890654", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082903989", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082943872", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083152707", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083163714", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-09", 
    "datePublishedReg": "2009-09-01", 
    "description": "A partial deletion of the adenovirus E3 region, comprising the overlapping 6.7K/gp19K genes, has been described for the incorporation of therapeutic genes in 'armed' oncolytic adenoviruses. This deletion allows the insertion of up to 2.5 kb genetic material into the virus and ensures strong expression of transgenes without reducing the replication and cytolytic potency of viruses in vitro. E3-gp19K and 6.7K proteins are involved in avoiding recognition and elimination of infected cells by the host immune system. Therefore, we have studied the effect of this deletion on the replication and transgene expression of the virus in immunocompetent models based on Syrian hamsters. Tumors were established by intrahepatic injection of pancreatic cancer cells with moderate (HaP-T1, HP-1) or low (H2T) permissivity for adenovirus replication. The wild-type human adenovirus 5 (Ad5) or a modified version containing the luciferase gene in the E3-6.7K/gp19K locus (Ad-WTLuc) were injected intratumorally. We found that elimination of Ad-WTLuc was faster than Ad5 in HaP-T1 and HP-1 tumors. In contrast, no differences were observed when the same tumor was established in severely immunocompromised NOD-scid IL2Rgamma(null) mice. In addition, virus-mediated luciferase expression was more stable in these animals. These results suggest that the lack of E3-6.7K/gp19K genes may accelerate the clearance of oncolytic adenoviruses in some immunocompetent tumor models.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/cgt.2009.12", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1112008", 
        "issn": [
          "0929-1903", 
          "1476-5500"
        ], 
        "name": "Cancer Gene Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "16"
      }
    ], 
    "name": "Deletion of the E3-6.7K/gp19K region reduces the persistence of wild-type adenovirus in a permissive tumor model in Syrian hamsters", 
    "pagination": "cgt200912", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b9b0425349c562e4ec91264654257ad68d0fe073aae4f9539989b1ad7c78d957"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19229289"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9432230"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/cgt.2009.12"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038981909"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/cgt.2009.12", 
      "https://app.dimensions.ai/details/publication/pub.1038981909"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000527.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/cgt200912"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/cgt.2009.12'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/cgt.2009.12'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/cgt.2009.12'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/cgt.2009.12'


 

This table displays all metadata directly associated to this object as RDF triples.

285 TRIPLES      21 PREDICATES      78 URIs      40 LITERALS      28 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/cgt.2009.12 schema:about N13da8ea05ee348c48b6bb38dc57f7226
2 N2924aa2c95ad4f1f9d621d520ae8c153
3 N329c884b9eef44a4b7d8cd1f63a0b149
4 N33d313d7b3384a7983ba493a5955964f
5 N362160a762694cf0b952940e3ff5fb5a
6 N41539c1bbdc249b2be9c20e8031f3cf5
7 N4cd269ba3025411b9b1f170c40adf3cb
8 N4f37ca5654a94a4b9c7144e1c233517d
9 N56e3225313514295bc56167715f130ea
10 N7db76f77e08c4969be8790112c195507
11 N83335dc9a1aa45348b099694d6f471d9
12 N87599a3fbb954f9c93f01f5032aaa6eb
13 N985bcb6b4e78414ca06fcd082eced6c7
14 N990bf6d361864a8584f05597adb279a2
15 Na0a593d82d4e41369f0f4bf22ca747d2
16 Nba9ff5e68fa548f5bab11a93fb43ea2c
17 Nbed96fbd4a3c41279078c40546b4a143
18 Ne02ddbdcbe334c71abfd279e354e7214
19 Ne24a6a06c41c4d8e9bad0426ce840f5c
20 anzsrc-for:11
21 anzsrc-for:1107
22 schema:author Nbd0dbb9381d54ae191781c951e811da1
23 schema:citation sg:pub.10.1007/978-3-540-75647-7_3
24 sg:pub.10.1007/978-3-642-79496-4_13
25 sg:pub.10.1007/bf01487691
26 sg:pub.10.1007/bf02924367
27 sg:pub.10.1038/nbt887
28 sg:pub.10.1038/sj.cgt.7700542
29 sg:pub.10.1038/sj.gt.3301507
30 sg:pub.10.1038/sj.gt.3301508
31 sg:pub.10.1038/sj.gt.3301509
32 sg:pub.10.1038/sj.onc.1209037
33 https://app.dimensions.ai/details/publication/pub.1082778756
34 https://app.dimensions.ai/details/publication/pub.1082890654
35 https://app.dimensions.ai/details/publication/pub.1082903989
36 https://app.dimensions.ai/details/publication/pub.1082943872
37 https://app.dimensions.ai/details/publication/pub.1083152707
38 https://app.dimensions.ai/details/publication/pub.1083163714
39 https://doi.org/10.1002/jgm.495
40 https://doi.org/10.1016/j.vetpar.2006.04.016
41 https://doi.org/10.1016/s1525-0016(03)00199-0
42 https://doi.org/10.1038/mt.2008.162
43 https://doi.org/10.1073/pnas.84.5.1356
44 https://doi.org/10.1074/jbc.m008218200
45 https://doi.org/10.1089/104303402760293574
46 https://doi.org/10.1089/hum.2007.017
47 https://doi.org/10.1128/jvi.76.4.1578-1587.2002
48 https://doi.org/10.1128/jvi.77.14.7764-7778.2003
49 https://doi.org/10.1128/jvi.78.22.12297-12307.2004
50 https://doi.org/10.1128/jvi.79.9.5455-5465.2005
51 https://doi.org/10.1158/0008-5472.can-05-3497
52 https://doi.org/10.4049/jimmunol.174.10.6477
53 schema:datePublished 2009-09
54 schema:datePublishedReg 2009-09-01
55 schema:description A partial deletion of the adenovirus E3 region, comprising the overlapping 6.7K/gp19K genes, has been described for the incorporation of therapeutic genes in 'armed' oncolytic adenoviruses. This deletion allows the insertion of up to 2.5 kb genetic material into the virus and ensures strong expression of transgenes without reducing the replication and cytolytic potency of viruses in vitro. E3-gp19K and 6.7K proteins are involved in avoiding recognition and elimination of infected cells by the host immune system. Therefore, we have studied the effect of this deletion on the replication and transgene expression of the virus in immunocompetent models based on Syrian hamsters. Tumors were established by intrahepatic injection of pancreatic cancer cells with moderate (HaP-T1, HP-1) or low (H2T) permissivity for adenovirus replication. The wild-type human adenovirus 5 (Ad5) or a modified version containing the luciferase gene in the E3-6.7K/gp19K locus (Ad-WTLuc) were injected intratumorally. We found that elimination of Ad-WTLuc was faster than Ad5 in HaP-T1 and HP-1 tumors. In contrast, no differences were observed when the same tumor was established in severely immunocompromised NOD-scid IL2Rgamma(null) mice. In addition, virus-mediated luciferase expression was more stable in these animals. These results suggest that the lack of E3-6.7K/gp19K genes may accelerate the clearance of oncolytic adenoviruses in some immunocompetent tumor models.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree true
59 schema:isPartOf N6c88f42a561447f5b0283b68c0ceff89
60 Nc1978821026b4c68b65e2978e77e9c0c
61 sg:journal.1112008
62 schema:name Deletion of the E3-6.7K/gp19K region reduces the persistence of wild-type adenovirus in a permissive tumor model in Syrian hamsters
63 schema:pagination cgt200912
64 schema:productId N4663a9b17a8d4c29a057ace674d21828
65 N4dca7daaea2e44d9a363ea39f65a1ef5
66 N56231d9c161d403faf5db636f56a9a8f
67 N6502e2921c4347b6b3254ecf9c2e1e7f
68 Na94272bce91f48a797c26ed8917bcc3c
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038981909
70 https://doi.org/10.1038/cgt.2009.12
71 schema:sdDatePublished 2019-04-10T14:13
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N642aa8e8dde6419d84ad1dd8d757ece9
74 schema:url http://www.nature.com/articles/cgt200912
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N13da8ea05ee348c48b6bb38dc57f7226 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Xenograft Model Antitumor Assays
80 rdf:type schema:DefinedTerm
81 N2924aa2c95ad4f1f9d621d520ae8c153 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Immunocompetence
83 rdf:type schema:DefinedTerm
84 N29672a1bdf7448dbb460868e753e25dc rdf:first sg:person.0770060660.33
85 rdf:rest N9ce368ecaa6244118daf9b54953c35ef
86 N30f849dbcb9946bfacd8ca197771ceda rdf:first sg:person.01142515214.09
87 rdf:rest N29672a1bdf7448dbb460868e753e25dc
88 N329c884b9eef44a4b7d8cd1f63a0b149 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Genes, Reporter
90 rdf:type schema:DefinedTerm
91 N33d313d7b3384a7983ba493a5955964f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Humans
93 rdf:type schema:DefinedTerm
94 N362160a762694cf0b952940e3ff5fb5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Disease Models, Animal
96 rdf:type schema:DefinedTerm
97 N41539c1bbdc249b2be9c20e8031f3cf5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Cell Line
99 rdf:type schema:DefinedTerm
100 N461ecc17956c4efe8a0f461143b1427f rdf:first sg:person.01065406061.80
101 rdf:rest Nb1abab90b60a4eda9b79c67bb175e9df
102 N4663a9b17a8d4c29a057ace674d21828 schema:name readcube_id
103 schema:value b9b0425349c562e4ec91264654257ad68d0fe073aae4f9539989b1ad7c78d957
104 rdf:type schema:PropertyValue
105 N4cd269ba3025411b9b1f170c40adf3cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Virus Replication
107 rdf:type schema:DefinedTerm
108 N4dca7daaea2e44d9a363ea39f65a1ef5 schema:name doi
109 schema:value 10.1038/cgt.2009.12
110 rdf:type schema:PropertyValue
111 N4f37ca5654a94a4b9c7144e1c233517d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Transgenes
113 rdf:type schema:DefinedTerm
114 N56231d9c161d403faf5db636f56a9a8f schema:name dimensions_id
115 schema:value pub.1038981909
116 rdf:type schema:PropertyValue
117 N56e3225313514295bc56167715f130ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Mesocricetus
119 rdf:type schema:DefinedTerm
120 N63e9cc740f23414a91f383ef66b7148e rdf:first sg:person.0717423526.23
121 rdf:rest N461ecc17956c4efe8a0f461143b1427f
122 N642aa8e8dde6419d84ad1dd8d757ece9 schema:name Springer Nature - SN SciGraph project
123 rdf:type schema:Organization
124 N6502e2921c4347b6b3254ecf9c2e1e7f schema:name nlm_unique_id
125 schema:value 9432230
126 rdf:type schema:PropertyValue
127 N6c88f42a561447f5b0283b68c0ceff89 schema:volumeNumber 16
128 rdf:type schema:PublicationVolume
129 N7db76f77e08c4969be8790112c195507 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Cricetinae
131 rdf:type schema:DefinedTerm
132 N83335dc9a1aa45348b099694d6f471d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Oncolytic Virotherapy
134 rdf:type schema:DefinedTerm
135 N87599a3fbb954f9c93f01f5032aaa6eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Genome, Viral
137 rdf:type schema:DefinedTerm
138 N985bcb6b4e78414ca06fcd082eced6c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Gene Deletion
140 rdf:type schema:DefinedTerm
141 N990bf6d361864a8584f05597adb279a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Mice
143 rdf:type schema:DefinedTerm
144 N9ce368ecaa6244118daf9b54953c35ef rdf:first sg:person.01017272661.71
145 rdf:rest rdf:nil
146 Na0a593d82d4e41369f0f4bf22ca747d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Adenovirus E3 Proteins
148 rdf:type schema:DefinedTerm
149 Na94272bce91f48a797c26ed8917bcc3c schema:name pubmed_id
150 schema:value 19229289
151 rdf:type schema:PropertyValue
152 Nb1abab90b60a4eda9b79c67bb175e9df rdf:first sg:person.01034562125.08
153 rdf:rest N30f849dbcb9946bfacd8ca197771ceda
154 Nba9ff5e68fa548f5bab11a93fb43ea2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Adenoviridae
156 rdf:type schema:DefinedTerm
157 Nbd0dbb9381d54ae191781c951e811da1 rdf:first sg:person.0603175126.69
158 rdf:rest N63e9cc740f23414a91f383ef66b7148e
159 Nbed96fbd4a3c41279078c40546b4a143 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Neoplasms
161 rdf:type schema:DefinedTerm
162 Nc1978821026b4c68b65e2978e77e9c0c schema:issueNumber 9
163 rdf:type schema:PublicationIssue
164 Ne02ddbdcbe334c71abfd279e354e7214 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Gene Expression Regulation, Viral
166 rdf:type schema:DefinedTerm
167 Ne24a6a06c41c4d8e9bad0426ce840f5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Animals
169 rdf:type schema:DefinedTerm
170 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
171 schema:name Medical and Health Sciences
172 rdf:type schema:DefinedTerm
173 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
174 schema:name Immunology
175 rdf:type schema:DefinedTerm
176 sg:journal.1112008 schema:issn 0929-1903
177 1476-5500
178 schema:name Cancer Gene Therapy
179 rdf:type schema:Periodical
180 sg:person.01017272661.71 schema:affiliation https://www.grid.ac/institutes/grid.5924.a
181 schema:familyName Hernandez-Alcoceba
182 schema:givenName R
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01017272661.71
184 rdf:type schema:Person
185 sg:person.01034562125.08 schema:affiliation https://www.grid.ac/institutes/grid.5924.a
186 schema:familyName Lamas
187 schema:givenName O
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034562125.08
189 rdf:type schema:Person
190 sg:person.01065406061.80 schema:affiliation https://www.grid.ac/institutes/grid.5924.a
191 schema:familyName Alzuguren
192 schema:givenName P
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065406061.80
194 rdf:type schema:Person
195 sg:person.01142515214.09 schema:affiliation https://www.grid.ac/institutes/grid.5924.a
196 schema:familyName Aldabe
197 schema:givenName R
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142515214.09
199 rdf:type schema:Person
200 sg:person.0603175126.69 schema:affiliation https://www.grid.ac/institutes/grid.5924.a
201 schema:familyName Bortolanza
202 schema:givenName S
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603175126.69
204 rdf:type schema:Person
205 sg:person.0717423526.23 schema:affiliation https://www.grid.ac/institutes/grid.5924.a
206 schema:familyName Bunuales
207 schema:givenName M
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717423526.23
209 rdf:type schema:Person
210 sg:person.0770060660.33 schema:affiliation https://www.grid.ac/institutes/grid.413448.e
211 schema:familyName Prieto
212 schema:givenName J
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770060660.33
214 rdf:type schema:Person
215 sg:pub.10.1007/978-3-540-75647-7_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021537765
216 https://doi.org/10.1007/978-3-540-75647-7_3
217 rdf:type schema:CreativeWork
218 sg:pub.10.1007/978-3-642-79496-4_13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007732172
219 https://doi.org/10.1007/978-3-642-79496-4_13
220 rdf:type schema:CreativeWork
221 sg:pub.10.1007/bf01487691 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000233423
222 https://doi.org/10.1007/bf01487691
223 rdf:type schema:CreativeWork
224 sg:pub.10.1007/bf02924367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075325265
225 https://doi.org/10.1007/bf02924367
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nbt887 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043212288
228 https://doi.org/10.1038/nbt887
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/sj.cgt.7700542 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008482065
231 https://doi.org/10.1038/sj.cgt.7700542
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/sj.gt.3301507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049636162
234 https://doi.org/10.1038/sj.gt.3301507
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/sj.gt.3301508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007414000
237 https://doi.org/10.1038/sj.gt.3301508
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/sj.gt.3301509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010537663
240 https://doi.org/10.1038/sj.gt.3301509
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/sj.onc.1209037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025367553
243 https://doi.org/10.1038/sj.onc.1209037
244 rdf:type schema:CreativeWork
245 https://app.dimensions.ai/details/publication/pub.1082778756 schema:CreativeWork
246 https://app.dimensions.ai/details/publication/pub.1082890654 schema:CreativeWork
247 https://app.dimensions.ai/details/publication/pub.1082903989 schema:CreativeWork
248 https://app.dimensions.ai/details/publication/pub.1082943872 schema:CreativeWork
249 https://app.dimensions.ai/details/publication/pub.1083152707 schema:CreativeWork
250 https://app.dimensions.ai/details/publication/pub.1083163714 schema:CreativeWork
251 https://doi.org/10.1002/jgm.495 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036710781
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/j.vetpar.2006.04.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027608718
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/s1525-0016(03)00199-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022590672
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1038/mt.2008.162 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007700261
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1073/pnas.84.5.1356 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029403876
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1074/jbc.m008218200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011814898
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1089/104303402760293574 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059202491
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1089/hum.2007.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272996
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1128/jvi.76.4.1578-1587.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000666510
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1128/jvi.77.14.7764-7778.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050881287
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1128/jvi.78.22.12297-12307.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022723576
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1128/jvi.79.9.5455-5465.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050164659
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1158/0008-5472.can-05-3497 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021463586
276 rdf:type schema:CreativeWork
277 https://doi.org/10.4049/jimmunol.174.10.6477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010296345
278 rdf:type schema:CreativeWork
279 https://www.grid.ac/institutes/grid.413448.e schema:alternateName Instituto de Salud Carlos III
280 schema:name CIBERehd, University Clinic, Navarra, Spain
281 Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain
282 rdf:type schema:Organization
283 https://www.grid.ac/institutes/grid.5924.a schema:alternateName University of Navarra
284 schema:name Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain
285 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...