Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-05-12

AUTHORS

Rui Liu, Ling Chen, Yanping Jiang, Zhihua Zhou, Gen Zou

ABSTRACT

Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a powerful genome-editing method that facilitates genetic alterations of genomes in a variety of organisms. However, a genome-editing approach has not been reported in filamentous fungi. Here, we demonstrated the establishment of a CRISPR/Cas9 system in the filamentous fungus Trichoderma reesei by specific codon optimization and in vitro RNA transcription. It was shown that the CRISPR/Cas9 system was controllable and conditional through inducible Cas9 expression. This system generated site-specific mutations in target genes through efficient homologous recombination, even using short homology arms. This system also provided an applicable and promising approach to targeting multiple genes simultaneously. Our results illustrate that the CRISPR/Cas9 system is a powerful genome-manipulating tool for T. reesei and most likely for other filamentous fungal species, which may accelerate studies on functional genomics and strain improvement in these filamentous fungi. More... »

PAGES

15007

Journal

TITLE

Cell Discovery

ISSUE

1

VOLUME

1

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/celldisc.2015.7

DOI

http://dx.doi.org/10.1038/celldisc.2015.7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029390352

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27462408


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