The interaction between mesenchymal stem cells and steroids during inflammation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-01-23

AUTHORS

X Chen, Y Gan, W Li, J Su, Y Zhang, Y Huang, A I Roberts, Y Han, J Li, Y Wang, Y Shi

ABSTRACT

Mesenchymal stem cells (MSCs) are believed to exert their regenerative effects through differentiation and modulation of inflammatory responses. However, the relationship between the severity of inflammation and stem cell-mediated tissue repair has not been formally investigated. In this study, we applied different concentrations of dexamethasone (Dex) to anti-CD3-activated splenocyte cultured with or without MSCs. As expected, Dex exhibited a classical dose-dependent inhibition of T-cell proliferation. Surprisingly, although MSCs also blocked T-cell proliferation, the presence of Dex unexpectedly showed a dose-dependent reversion of T-cell proliferation. This effect of Dex was found to be exerted through interfering STAT1 phosphorylation-prompted expression of inducible nitric oxide synthase (iNOS). Interestingly, inflammation-induced chemokines in MSCs was unaffected. To test the role of inflammation severity in stem cell-mediated tissue repair, we employed mice with carbon tetrachloride-induced advanced liver fibrosis and found that although MSCs alone were effective, concurrent administration of Dex abrogated the therapeutic effects of MSCs on fibrin deposition, serum levels of bilirubin, albumin, and aminotransferases, as well as T-lymphocyte infiltration, especially IFN-γ+CD4+ and IL-17A+CD4+T cells. Likewise, iNOS−/− MSCs, which produce chemokines but not nitric oxide under inflammatory conditions, are ineffective in treating advanced liver fibrosis. Therefore, inflammation has a critical role in MSC-mediated tissue repair. In addition, concomitant application of MSCs with steroids should be avoided. More... »

PAGES

e1009-e1009

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/cddis.2013.537

DOI

http://dx.doi.org/10.1038/cddis.2013.537

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043054441

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24457953


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33 schema:description Mesenchymal stem cells (MSCs) are believed to exert their regenerative effects through differentiation and modulation of inflammatory responses. However, the relationship between the severity of inflammation and stem cell-mediated tissue repair has not been formally investigated. In this study, we applied different concentrations of dexamethasone (Dex) to anti-CD3-activated splenocyte cultured with or without MSCs. As expected, Dex exhibited a classical dose-dependent inhibition of T-cell proliferation. Surprisingly, although MSCs also blocked T-cell proliferation, the presence of Dex unexpectedly showed a dose-dependent reversion of T-cell proliferation. This effect of Dex was found to be exerted through interfering STAT1 phosphorylation-prompted expression of inducible nitric oxide synthase (iNOS). Interestingly, inflammation-induced chemokines in MSCs was unaffected. To test the role of inflammation severity in stem cell-mediated tissue repair, we employed mice with carbon tetrachloride-induced advanced liver fibrosis and found that although MSCs alone were effective, concurrent administration of Dex abrogated the therapeutic effects of MSCs on fibrin deposition, serum levels of bilirubin, albumin, and aminotransferases, as well as T-lymphocyte infiltration, especially IFN-γ+CD4+ and IL-17A+CD4+T cells. Likewise, iNOS−/− MSCs, which produce chemokines but not nitric oxide under inflammatory conditions, are ineffective in treating advanced liver fibrosis. Therefore, inflammation has a critical role in MSC-mediated tissue repair. In addition, concomitant application of MSCs with steroids should be avoided.
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40 IFN
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42 T cell proliferation
43 T lymphocyte infiltration
44 addition
45 administration
46 advanced liver fibrosis
47 albumin
48 aminotransferase
49 applications
50 bilirubin
51 carbon
52 cells
53 chemokines
54 concentration
55 concomitant application
56 concurrent administration
57 conditions
58 critical role
59 deposition
60 dexamethasone
61 different concentrations
62 differentiation
63 dose-dependent inhibition
64 dose-dependent reversion
65 effect
66 effect of Dex
67 expression
68 fibrin deposition
69 fibrosis
70 inducible nitric oxide synthase
71 infiltration
72 inflammation
73 inflammation severity
74 inflammatory conditions
75 inflammatory response
76 inhibition
77 interaction
78 levels
79 liver fibrosis
80 mesenchymal stem cells
81 mice
82 modulation
83 nitric oxide
84 nitric oxide synthase
85 oxide
86 oxide synthase
87 presence
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91 relationship
92 repair
93 response
94 reversion
95 role
96 serum levels
97 severity
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99 splenocytes
100 stem cells
101 steroids
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