Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12-07

AUTHORS

Gloria Allocca, Anjali P Kusumbe, Saravana K Ramasamy, Ning Wang

ABSTRACT

Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples. More... »

PAGES

851

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/bonekey.2016.84

DOI

http://dx.doi.org/10.1038/bonekey.2016.84

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031089556

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28018584


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