Anomalous retinoblastoma protein expression in Sternberg-Reed cells in Hodgkin's disease: a comparative study with p53 and Ki67 expression View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1996-10

AUTHORS

M Sánchez-Beato, J C Martínez-Montero, T A Doussis-Anagnostopoulou, K C Gatter, J García, J F García, E LLoret, M A Piris

ABSTRACT

Retinoblastoma (Rb) tumour-suppressor protein plays a critical role in cell cycle control. Rb inactivation is a frequent phenomenon in tumours of different cell lineages, in which the absence of Rb protein has been considered to be a marker of Rb disregulation. We used modern immunohistochemical techniques to study the expression of Rb protein in a large series of 130 patients with Hodgkin's disease. Simultaneously, Western blot was used to analyse a more restricted group (12 patients) to confirm the immunohistochemical results and to clarify the phosphorylation status of Rb protein. As the level of Rb expression varied according to cell cycle stage, we also performed immunostaining for Ki67, a protein present in proliferating cells. To make comparison possible, we first characterised the amount and phosphorylation status of Rb protein in reactive lymphoid tissue and phytohaemagglutinin (PHA)-stimulated lymphocytes. The presence of p53 in Sternberg-Reed cells was also included in the study, as both proteins (p53 and Rb) have been found to be closely associated in cell cycle control. PHA-stimulated peripheral blood lymphocytes showed a parallel increase in Rb and cell cycle progression, together with progressive Rb phosphorylation. In reactive lymphoid tissue there was also a clear correlation between Rb expression and the Ki67 proliferation index (R = 0.96, P = 0.038). When analysing Hodgkin's disease samples, a clear difference emerges between cases of nodular lymphocyte predominance, which preserve the relationship between Rb and Ki67 expression (r = 0.8727, P = 0.000), and classical forms of Hodgkin's disease (nodular sclerosis and mixed cellularity), which display a strong deviation from this pattern. Two main anomalies were found: (1) One group of 21/130 cases with partial or total loss of Rb protein expression, which could reflect the existence of genetic alterations, or an altered transcriptional or translational regulation of Rb gene. (2) Another group with an abnormally high Rb/Ki67 ratio, which could support conflicting interpretations: (i) excess Rb protein for controlling cell cycle progression; or (ii) adhesion of Rb protein to other cellular or viral proteins, such as p53 and MDM2. The results of this study indicate an anomalous pattern of expression of Rb in classical forms of Hodgkin's disease, and suggest the possibility of undertaking functional studies (E1A adhesion, p16 expression) with the aim of better characterising the status of Rb protein, and correlating these findings with clinical course in Hodgkin's disease patients. More... »

PAGES

1056

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/bjc.1996.489

DOI

http://dx.doi.org/10.1038/bjc.1996.489

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036183892

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8855974


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hodgkin Disease", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ki-67 Antigen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphoid Tissue", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reed-Sternberg Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Retinoblastoma Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Suppressor Protein p53", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Department of Pathology, Hospital V. de la Salud, Toledo, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "S\u00e1nchez-Beato", 
        "givenName": "M", 
        "id": "sg:person.01026644115.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026644115.63"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Mart\u00ednez-Montero", 
        "givenName": "J C", 
        "id": "sg:person.01115753461.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115753461.94"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Doussis-Anagnostopoulou", 
        "givenName": "T A", 
        "type": "Person"
      }, 
      {
        "familyName": "Gatter", 
        "givenName": "K C", 
        "id": "sg:person.01024131223.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024131223.95"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Garc\u00eda", 
        "givenName": "J", 
        "id": "sg:person.011124702042.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011124702042.53"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Garc\u00eda", 
        "givenName": "J F", 
        "id": "sg:person.013242743702.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013242743702.55"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "LLoret", 
        "givenName": "E", 
        "id": "sg:person.0654226146.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654226146.90"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Piris", 
        "givenName": "M A", 
        "id": "sg:person.011442442264.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011442442264.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1056/nejm199011223232105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001952774"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/371021a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003384180", 
          "https://doi.org/10.1038/371021a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.7.2775", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003839721"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711770106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015195697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711770106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015195697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.12.5455", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016477264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/334124a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019612455", 
          "https://doi.org/10.1038/334124a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(91)90557-f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023113546"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cyto.990030503", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024745379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cyto.990030503", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024745379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(88)90559-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026329406"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(89)90517-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030112583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/375694a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043400439", 
          "https://doi.org/10.1038/375694a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.12.5320", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045383596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711690404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045521119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711690404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045521119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0169-5002(95)96300-k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047114128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(89)90508-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050118781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711680404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051457357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711680404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051457357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng0894-480", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051798898", 
          "https://doi.org/10.1038/ng0894-480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/jcp.48.2.151", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053358275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/84.16.1251", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059817370"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2521957", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062542263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2537532", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062542761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2588006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062545142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077346916", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb05006.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077431412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077653338", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077673766", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077819805", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077833328", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077941175", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077986982", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078024967", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb08018.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078039711"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078042731", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078974069", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079286587", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082630897", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082664687", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082727135", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082820728", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-10", 
    "datePublishedReg": "1996-10-01", 
    "description": "Retinoblastoma (Rb) tumour-suppressor protein plays a critical role in cell cycle control. Rb inactivation is a frequent phenomenon in tumours of different cell lineages, in which the absence of Rb protein has been considered to be a marker of Rb disregulation. We used modern immunohistochemical techniques to study the expression of Rb protein in a large series of 130 patients with Hodgkin's disease. Simultaneously, Western blot was used to analyse a more restricted group (12 patients) to confirm the immunohistochemical results and to clarify the phosphorylation status of Rb protein. As the level of Rb expression varied according to cell cycle stage, we also performed immunostaining for Ki67, a protein present in proliferating cells. To make comparison possible, we first characterised the amount and phosphorylation status of Rb protein in reactive lymphoid tissue and phytohaemagglutinin (PHA)-stimulated lymphocytes. The presence of p53 in Sternberg-Reed cells was also included in the study, as both proteins (p53 and Rb) have been found to be closely associated in cell cycle control. PHA-stimulated peripheral blood lymphocytes showed a parallel increase in Rb and cell cycle progression, together with progressive Rb phosphorylation. In reactive lymphoid tissue there was also a clear correlation between Rb expression and the Ki67 proliferation index (R = 0.96, P = 0.038). When analysing Hodgkin's disease samples, a clear difference emerges between cases of nodular lymphocyte predominance, which preserve the relationship between Rb and Ki67 expression (r = 0.8727, P = 0.000), and classical forms of Hodgkin's disease (nodular sclerosis and mixed cellularity), which display a strong deviation from this pattern. Two main anomalies were found: (1) One group of 21/130 cases with partial or total loss of Rb protein expression, which could reflect the existence of genetic alterations, or an altered transcriptional or translational regulation of Rb gene. (2) Another group with an abnormally high Rb/Ki67 ratio, which could support conflicting interpretations: (i) excess Rb protein for controlling cell cycle progression; or (ii) adhesion of Rb protein to other cellular or viral proteins, such as p53 and MDM2. The results of this study indicate an anomalous pattern of expression of Rb in classical forms of Hodgkin's disease, and suggest the possibility of undertaking functional studies (E1A adhesion, p16 expression) with the aim of better characterising the status of Rb protein, and correlating these findings with clinical course in Hodgkin's disease patients.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/bjc.1996.489", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1017082", 
        "issn": [
          "0007-0920", 
          "1532-1827"
        ], 
        "name": "British Journal of Cancer", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "74"
      }
    ], 
    "name": "Anomalous retinoblastoma protein expression in Sternberg-Reed cells in Hodgkin's disease: a comparative study with p53 and Ki67 expression", 
    "pagination": "1056", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ac5d9bbd7f86d39868caaa4a3c7905441b50eea0220aaca3d358534e1c53c255"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8855974"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0370635"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/bjc.1996.489"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036183892"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/bjc.1996.489", 
      "https://app.dimensions.ai/details/publication/pub.1036183892"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000359_0000000359/records_29183_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/bjc1996489"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/bjc.1996.489'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/bjc.1996.489'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/bjc.1996.489'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/bjc.1996.489'


 

This table displays all metadata directly associated to this object as RDF triples.

247 TRIPLES      21 PREDICATES      76 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/bjc.1996.489 schema:about N187a639172604670b91b99e50222f032
2 N230380e1660849b4988fad64abc023e1
3 N4dd38f1f8aa1437686bb684f7924bbdd
4 N582c7922107f472ba697aa4f7679f17d
5 N675bf3242e3149daafe755fd02d11383
6 N9cb609aa5d844bd0bcffe4bab8d3c4ff
7 Nc8aa765ed77a4d5fba8f4bb359059599
8 Nedae596296104fee8b41d67411c47023
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author Ne62f142cf0084b849806ef1710fb8fe8
12 schema:citation sg:pub.10.1038/334124a0
13 sg:pub.10.1038/371021a0
14 sg:pub.10.1038/375694a0
15 sg:pub.10.1038/ng0894-480
16 https://app.dimensions.ai/details/publication/pub.1077346916
17 https://app.dimensions.ai/details/publication/pub.1077653338
18 https://app.dimensions.ai/details/publication/pub.1077673766
19 https://app.dimensions.ai/details/publication/pub.1077819805
20 https://app.dimensions.ai/details/publication/pub.1077833328
21 https://app.dimensions.ai/details/publication/pub.1077941175
22 https://app.dimensions.ai/details/publication/pub.1077986982
23 https://app.dimensions.ai/details/publication/pub.1078024967
24 https://app.dimensions.ai/details/publication/pub.1078042731
25 https://app.dimensions.ai/details/publication/pub.1078974069
26 https://app.dimensions.ai/details/publication/pub.1079286587
27 https://app.dimensions.ai/details/publication/pub.1082630897
28 https://app.dimensions.ai/details/publication/pub.1082664687
29 https://app.dimensions.ai/details/publication/pub.1082727135
30 https://app.dimensions.ai/details/publication/pub.1082820728
31 https://doi.org/10.1002/cyto.990030503
32 https://doi.org/10.1002/j.1460-2075.1991.tb05006.x
33 https://doi.org/10.1002/j.1460-2075.1991.tb08018.x
34 https://doi.org/10.1002/path.1711680404
35 https://doi.org/10.1002/path.1711690404
36 https://doi.org/10.1002/path.1711770106
37 https://doi.org/10.1016/0092-8674(88)90559-4
38 https://doi.org/10.1016/0092-8674(89)90508-4
39 https://doi.org/10.1016/0092-8674(89)90517-5
40 https://doi.org/10.1016/0092-8674(91)90557-f
41 https://doi.org/10.1016/0169-5002(95)96300-k
42 https://doi.org/10.1056/nejm199011223232105
43 https://doi.org/10.1073/pnas.87.7.2775
44 https://doi.org/10.1073/pnas.90.12.5455
45 https://doi.org/10.1073/pnas.91.12.5320
46 https://doi.org/10.1093/jnci/84.16.1251
47 https://doi.org/10.1126/science.2521957
48 https://doi.org/10.1126/science.2537532
49 https://doi.org/10.1126/science.2588006
50 https://doi.org/10.1136/jcp.48.2.151
51 schema:datePublished 1996-10
52 schema:datePublishedReg 1996-10-01
53 schema:description Retinoblastoma (Rb) tumour-suppressor protein plays a critical role in cell cycle control. Rb inactivation is a frequent phenomenon in tumours of different cell lineages, in which the absence of Rb protein has been considered to be a marker of Rb disregulation. We used modern immunohistochemical techniques to study the expression of Rb protein in a large series of 130 patients with Hodgkin's disease. Simultaneously, Western blot was used to analyse a more restricted group (12 patients) to confirm the immunohistochemical results and to clarify the phosphorylation status of Rb protein. As the level of Rb expression varied according to cell cycle stage, we also performed immunostaining for Ki67, a protein present in proliferating cells. To make comparison possible, we first characterised the amount and phosphorylation status of Rb protein in reactive lymphoid tissue and phytohaemagglutinin (PHA)-stimulated lymphocytes. The presence of p53 in Sternberg-Reed cells was also included in the study, as both proteins (p53 and Rb) have been found to be closely associated in cell cycle control. PHA-stimulated peripheral blood lymphocytes showed a parallel increase in Rb and cell cycle progression, together with progressive Rb phosphorylation. In reactive lymphoid tissue there was also a clear correlation between Rb expression and the Ki67 proliferation index (R = 0.96, P = 0.038). When analysing Hodgkin's disease samples, a clear difference emerges between cases of nodular lymphocyte predominance, which preserve the relationship between Rb and Ki67 expression (r = 0.8727, P = 0.000), and classical forms of Hodgkin's disease (nodular sclerosis and mixed cellularity), which display a strong deviation from this pattern. Two main anomalies were found: (1) One group of 21/130 cases with partial or total loss of Rb protein expression, which could reflect the existence of genetic alterations, or an altered transcriptional or translational regulation of Rb gene. (2) Another group with an abnormally high Rb/Ki67 ratio, which could support conflicting interpretations: (i) excess Rb protein for controlling cell cycle progression; or (ii) adhesion of Rb protein to other cellular or viral proteins, such as p53 and MDM2. The results of this study indicate an anomalous pattern of expression of Rb in classical forms of Hodgkin's disease, and suggest the possibility of undertaking functional studies (E1A adhesion, p16 expression) with the aim of better characterising the status of Rb protein, and correlating these findings with clinical course in Hodgkin's disease patients.
54 schema:genre research_article
55 schema:inLanguage en
56 schema:isAccessibleForFree true
57 schema:isPartOf Na2de52426ef246c98c7474c580121cd5
58 Nbd31c3bb211f417bb4e93dceda588395
59 sg:journal.1017082
60 schema:name Anomalous retinoblastoma protein expression in Sternberg-Reed cells in Hodgkin's disease: a comparative study with p53 and Ki67 expression
61 schema:pagination 1056
62 schema:productId N026ce90f833f4eddaf5ca50011ad3a47
63 N228d5d41d08f403096f2789e391bf10b
64 N98a769d17f15492c87ed949a7b34ca9d
65 Na5c32cda2c9241bd8987db8a259f60e4
66 Nebc641e84bd94126ba28198f1ee29bed
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036183892
68 https://doi.org/10.1038/bjc.1996.489
69 schema:sdDatePublished 2019-04-11T11:51
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher Na5d1e1b6efb04cad95de613cfa7a770b
72 schema:url https://www.nature.com/articles/bjc1996489
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N026ce90f833f4eddaf5ca50011ad3a47 schema:name doi
77 schema:value 10.1038/bjc.1996.489
78 rdf:type schema:PropertyValue
79 N073f100822e145d4b42b91cc00de3eea rdf:first sg:person.011124702042.53
80 rdf:rest N47e42a67dee144fa985fbfba4c9e69b6
81 N187a639172604670b91b99e50222f032 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Tumor Suppressor Protein p53
83 rdf:type schema:DefinedTerm
84 N228d5d41d08f403096f2789e391bf10b schema:name nlm_unique_id
85 schema:value 0370635
86 rdf:type schema:PropertyValue
87 N230380e1660849b4988fad64abc023e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Retinoblastoma Protein
89 rdf:type schema:DefinedTerm
90 N47e42a67dee144fa985fbfba4c9e69b6 rdf:first sg:person.013242743702.55
91 rdf:rest Ndad2ded025c14e5ab72208ec30221aec
92 N4dd38f1f8aa1437686bb684f7924bbdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Ki-67 Antigen
94 rdf:type schema:DefinedTerm
95 N582c7922107f472ba697aa4f7679f17d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Reed-Sternberg Cells
97 rdf:type schema:DefinedTerm
98 N675bf3242e3149daafe755fd02d11383 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Humans
100 rdf:type schema:DefinedTerm
101 N8d77a218259e4748afbf0f9482a41b25 schema:name Department of Pathology, Hospital V. de la Salud, Toledo, Spain.
102 rdf:type schema:Organization
103 N98a769d17f15492c87ed949a7b34ca9d schema:name pubmed_id
104 schema:value 8855974
105 rdf:type schema:PropertyValue
106 N9b7e65978a5f4a84846080496c845e9b rdf:first sg:person.011442442264.52
107 rdf:rest rdf:nil
108 N9cb609aa5d844bd0bcffe4bab8d3c4ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Lymphoid Tissue
110 rdf:type schema:DefinedTerm
111 Na2de52426ef246c98c7474c580121cd5 schema:issueNumber 7
112 rdf:type schema:PublicationIssue
113 Na5c32cda2c9241bd8987db8a259f60e4 schema:name dimensions_id
114 schema:value pub.1036183892
115 rdf:type schema:PropertyValue
116 Na5d1e1b6efb04cad95de613cfa7a770b schema:name Springer Nature - SN SciGraph project
117 rdf:type schema:Organization
118 Nb3c3b319f16c458e9e1b92fcbefda8c1 rdf:first sg:person.01024131223.95
119 rdf:rest N073f100822e145d4b42b91cc00de3eea
120 Nbd31c3bb211f417bb4e93dceda588395 schema:volumeNumber 74
121 rdf:type schema:PublicationVolume
122 Nc8aa765ed77a4d5fba8f4bb359059599 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Hodgkin Disease
124 rdf:type schema:DefinedTerm
125 Ndad2ded025c14e5ab72208ec30221aec rdf:first sg:person.0654226146.90
126 rdf:rest N9b7e65978a5f4a84846080496c845e9b
127 Ne62f142cf0084b849806ef1710fb8fe8 rdf:first sg:person.01026644115.63
128 rdf:rest Ne6fa6477d78546f0979cfa0bd7b26de8
129 Ne6fa6477d78546f0979cfa0bd7b26de8 rdf:first sg:person.01115753461.94
130 rdf:rest Nfe00b0be35cf4678abb726ca48a9d5b3
131 Nebc641e84bd94126ba28198f1ee29bed schema:name readcube_id
132 schema:value ac5d9bbd7f86d39868caaa4a3c7905441b50eea0220aaca3d358534e1c53c255
133 rdf:type schema:PropertyValue
134 Nedae596296104fee8b41d67411c47023 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Neoplasm Proteins
136 rdf:type schema:DefinedTerm
137 Nfae8c3705ca84f2c8dd0facea6068ac0 schema:familyName Doussis-Anagnostopoulou
138 schema:givenName T A
139 rdf:type schema:Person
140 Nfe00b0be35cf4678abb726ca48a9d5b3 rdf:first Nfae8c3705ca84f2c8dd0facea6068ac0
141 rdf:rest Nb3c3b319f16c458e9e1b92fcbefda8c1
142 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biological Sciences
144 rdf:type schema:DefinedTerm
145 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
146 schema:name Biochemistry and Cell Biology
147 rdf:type schema:DefinedTerm
148 sg:journal.1017082 schema:issn 0007-0920
149 1532-1827
150 schema:name British Journal of Cancer
151 rdf:type schema:Periodical
152 sg:person.01024131223.95 schema:familyName Gatter
153 schema:givenName K C
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024131223.95
155 rdf:type schema:Person
156 sg:person.01026644115.63 schema:affiliation N8d77a218259e4748afbf0f9482a41b25
157 schema:familyName Sánchez-Beato
158 schema:givenName M
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026644115.63
160 rdf:type schema:Person
161 sg:person.011124702042.53 schema:familyName García
162 schema:givenName J
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011124702042.53
164 rdf:type schema:Person
165 sg:person.01115753461.94 schema:familyName Martínez-Montero
166 schema:givenName J C
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115753461.94
168 rdf:type schema:Person
169 sg:person.011442442264.52 schema:familyName Piris
170 schema:givenName M A
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011442442264.52
172 rdf:type schema:Person
173 sg:person.013242743702.55 schema:familyName García
174 schema:givenName J F
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013242743702.55
176 rdf:type schema:Person
177 sg:person.0654226146.90 schema:familyName LLoret
178 schema:givenName E
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654226146.90
180 rdf:type schema:Person
181 sg:pub.10.1038/334124a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019612455
182 https://doi.org/10.1038/334124a0
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/371021a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003384180
185 https://doi.org/10.1038/371021a0
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/375694a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043400439
188 https://doi.org/10.1038/375694a0
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/ng0894-480 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051798898
191 https://doi.org/10.1038/ng0894-480
192 rdf:type schema:CreativeWork
193 https://app.dimensions.ai/details/publication/pub.1077346916 schema:CreativeWork
194 https://app.dimensions.ai/details/publication/pub.1077653338 schema:CreativeWork
195 https://app.dimensions.ai/details/publication/pub.1077673766 schema:CreativeWork
196 https://app.dimensions.ai/details/publication/pub.1077819805 schema:CreativeWork
197 https://app.dimensions.ai/details/publication/pub.1077833328 schema:CreativeWork
198 https://app.dimensions.ai/details/publication/pub.1077941175 schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1077986982 schema:CreativeWork
200 https://app.dimensions.ai/details/publication/pub.1078024967 schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1078042731 schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1078974069 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1079286587 schema:CreativeWork
204 https://app.dimensions.ai/details/publication/pub.1082630897 schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1082664687 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1082727135 schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1082820728 schema:CreativeWork
208 https://doi.org/10.1002/cyto.990030503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024745379
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1002/j.1460-2075.1991.tb05006.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077431412
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1002/j.1460-2075.1991.tb08018.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078039711
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1002/path.1711680404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051457357
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1002/path.1711690404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045521119
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1002/path.1711770106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015195697
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/0092-8674(88)90559-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026329406
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/0092-8674(89)90508-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050118781
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/0092-8674(89)90517-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030112583
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/0092-8674(91)90557-f schema:sameAs https://app.dimensions.ai/details/publication/pub.1023113546
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/0169-5002(95)96300-k schema:sameAs https://app.dimensions.ai/details/publication/pub.1047114128
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1056/nejm199011223232105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001952774
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1073/pnas.87.7.2775 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003839721
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1073/pnas.90.12.5455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016477264
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1073/pnas.91.12.5320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045383596
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1093/jnci/84.16.1251 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059817370
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1126/science.2521957 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062542263
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1126/science.2537532 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062542761
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1126/science.2588006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062545142
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1136/jcp.48.2.151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053358275
247 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...