Insulin-like growth factor I activates the invasion suppressor function of E-cadherin in MCF-7 human mammary carcinoma cells in vitro View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1993-08

AUTHORS

M E Bracke, B M Vyncke, E A Bruyneel, S J Vermeulen, G K De Bruyne, N A Van Larebeke, K Vleminckx, F M Van Roy, M M Mareel

ABSTRACT

The calcium-dependent cell-cell adhesion molecule E-cadherin has been shown to counteract invasion of epithelial neoplastic cells. Using three monoclonal antibodies, we have demonstrated the presence of E-cadherin at the surface of human MCF-7/6 mammary carcinoma cells by indirect immunofluorescence coupled to flow cytometry and by immunocytochemistry. Nevertheless, MCF-7/6 cells failed to aggregate in a medium containing 1.25 mM CaCl2, and they were invasive after confrontation with embryonic chick heart fragments in organ culture. Treatment of MCF-7/6 cells with 0.5 microgram ml-1 insulin-like growth factor I (IGF-I) led to homotypic aggregation within 5 to 10 min and inhibited invasion in vitro during at least 8 days. The effect of IGF-I on cellular aggregation was insensitive to cycloheximide. However, monoclonal antibodies that interfered with the function of either the IGF-I receptor (alpha IR3) or E-cadherin (HECD-1, MB2) blocked the effect of IGF-I on aggregation. The effects of IGF-I on aggregation and on invasion could be mimicked by 1 microgram ml-1 insulin, but not by 0.5 microgram ml-1 IGF-II. The insulin effects were presumably not mediated by the IGF-I receptor, since they could not be blocked by an antibody against this receptor (alpha IR3). Our results indicate that IGF-I activates the invasion suppressor role of E-cadherin in MCF-7/6 cells. More... »

PAGES

282

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/bjc.1993.329

DOI

http://dx.doi.org/10.1038/bjc.1993.329

DIMENSIONS

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PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8347483


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