Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells View Full Text


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Article Info

DATE

2014-07

AUTHORS

Jiao-jiao Xu, Si-yuan Wang, Ye Chen, Guang-ping Chen, Zai-quan Li, Xue-yan Shao, Liang Li, Wei Lu, Tian-yan Zhou

ABSTRACT

AIM: Dopamine receptors are present in the nervous system and also widely distributed in the periphery. The aim of this study was to investigate the role of D1 subtype dopamine receptors (DRD1) in the regulation of dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells. METHODS: HepG2 cells were treated with DRD1 agonists with or without DRD1 antagonist for 9 d. DRD1 and SULT2A1 mRNA expression, protein expression, and SULT2A1 activity were detected using RT-PCR, Western blotting and HPLC, respectively. The level of cAMP was measured using a commercial kit. RESULTS: All the 5 DR subtypes (DRD1-DRD5) were found to be expressed in HepG2 cells. Treatment of HepG2 cells with the specific DRD1 agonists SKF82958 (2.5 μmol/L) or SKF38393 (5 and 50 μmol/L) significantly increased the mRNA and protein expression of both DRD1 and SULT2A1, and increased SULT2A1 activity and cAMP levels. These effects were partially blocked by co-treatment with the specific DRD1 antagonist SCH23390 (2.5 μmol/L). In addition, transfection of HepG2 cells with DRD1-specific siRNAs decreased DRD1 mRNA expression by 40%, which resulted in the reduction of SULT2A1 mRNA expression by 60%, protein expression by 40%, and enzyme activity by 20%. CONCLUSION: DRD1 activation upregulates DRD1 and SULT2A1 expression and SULT2A1 activity in HepG2 cells, suggesting that the DRD1 subtype may be involved in the metabolism of drugs and xenobiotics through regulating SULT2A1. More... »

PAGES

aps201419

References to SciGraph publications

  • 2008-10-02. Neuroscience: Fragile dopamine in NATURE
  • 2002-03. Dopamine, hypertension and obesity in JOURNAL OF HUMAN HYPERTENSION
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    URI

    http://scigraph.springernature.com/pub.10.1038/aps.2014.19

    DOI

    http://dx.doi.org/10.1038/aps.2014.19

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1007325770

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24909515


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    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/aps.2014.19'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/aps.2014.19'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/aps.2014.19'

    RDF/XML is a standard XML format for linked data.

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