Cell-free translation reconstituted with purified components View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-08

AUTHORS

Y Shimizu, A Inoue, Y Tomari, T Suzuki, T Yokogawa, K Nishikawa, T Ueda

ABSTRACT

We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 microg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA). More... »

PAGES

751-755

Journal

TITLE

Nature Biotechnology

ISSUE

8

VOLUME

19

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/90802

    DOI

    http://dx.doi.org/10.1038/90802

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1032863741

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11479568


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