In silico predictions of Escherichia coli metabolic capabilities are consistent with experimental data View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-02

AUTHORS

Jeremy S. Edwards, Rafael U. Ibarra, Bernhard O. Palsson

ABSTRACT

A significant goal in the post-genome era is to relate the annotated genome sequence to the physiological functions of a cell. Working from the annotated genome sequence, as well as biochemical and physiological information, it is possible to reconstruct complete metabolic networks. Furthermore, computational methods have been developed to interpret and predict the optimal performance of a metabolic network under a range of growth conditions. We have tested the hypothesis that Escherichia coli uses its metabolism to grow at a maximal rate using the E. coli MG1655 metabolic reconstruction. Based on this hypothesis, we formulated experiments that describe the quantitative relationship between a primary carbon source (acetate or succinate) uptake rate, oxygen uptake rate, and maximal cellular growth rate. We found that the experimental data were consistent with the stated hypothesis, namely that the E. coli metabolic network is optimized to maximize growth under the experimental conditions considered. This study thus demonstrates how the combination of in silico and experimental biology can be used to obtain a quantitative genotype-phenotype relationship for metabolism in bacterial cells. More... »

PAGES

125

Journal

TITLE

Nature Biotechnology

ISSUE

2

VOLUME

19

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/84379

    DOI

    http://dx.doi.org/10.1038/84379

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015458337

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11175725


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