The proteolytic activity of tissue-plasminogen activator enhances NMDA receptor-mediated signaling View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-01

AUTHORS

Olivier Nicole, Fabian Docagne, Carine Ali, Isabelle Margaill, Peter Carmeliet, Eric T. MacKenzie, Denis Vivien, Alain Buisson

ABSTRACT

Tissue-plasminogen activator (t-PA) is now available for the treatment of thrombo-embolic stroke but adverse effects have been reported in some patients, particularly hemorrhaging. In contrast, the results of animal studies have indicated that t-PA could increase neuronal damage after focal cerebral ischemia. Here we report for the first time that t-PA potentiates signaling mediated by glutamatergic receptors by modifying the properties of the N-methyl-D-aspartate (NMDA) receptor. When depolarized, cortical neurons release bio-active t-PA that interacts with and cleaves the NR1 subunit of the NMDA receptor. Moreover, the treatment with recombinant t-PA leads to a 37% increase in NMDA-stimulated fura-2 fluorescence, which may reflect an increased NMDA-receptor function. These results were confirmed in vivo by the intrastriatal injection of recombinant-PA, which potentiated the excitotoxic lesions induced by NMDA. These data provide insight into the regulation of NMDA-receptor-mediated signaling and could initiate therapeutic strategies to improve the efficacy of t-PA treatment in man. More... »

PAGES

nm0101_59

Journal

TITLE

Nature Medicine

ISSUE

1

VOLUME

7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/83358

DOI

http://dx.doi.org/10.1038/83358

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048103862

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11135617


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Death", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrolysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ion Transport", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Potentials", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, N-Methyl-D-Aspartate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Plasminogen Activator", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 de Caen Basse-Normandie", 
          "id": "https://www.grid.ac/institutes/grid.412043.0", 
          "name": [
            "Universit\u00e9 de CAEN, UMR CNRS 6551, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN, Cedex, France", 
            "CEA-Universit\u00e9 LRA10V, UPRES EA 2609, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nicole", 
        "givenName": "Olivier", 
        "id": "sg:person.01117174340.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117174340.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 de Caen Basse-Normandie", 
          "id": "https://www.grid.ac/institutes/grid.412043.0", 
          "name": [
            "Universit\u00e9 de CAEN, UMR CNRS 6551, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN, Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Docagne", 
        "givenName": "Fabian", 
        "id": "sg:person.01125221124.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125221124.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 de Caen Basse-Normandie", 
          "id": "https://www.grid.ac/institutes/grid.412043.0", 
          "name": [
            "Universit\u00e9 de CAEN, UMR CNRS 6551, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN, Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ali", 
        "givenName": "Carine", 
        "id": "sg:person.01235307632.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235307632.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Paris Descartes University", 
          "id": "https://www.grid.ac/institutes/grid.10992.33", 
          "name": [
            "Laboratoire de pharmacologie, Facult\u00e9 des Sciences Pharmaceutiques et Biologiques, Universit\u00e9 Ren\u00e9 Descartes, Paris F-75006, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Margaill", 
        "givenName": "Isabelle", 
        "id": "sg:person.01065077153.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065077153.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Flanders Institute for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.11486.3a", 
          "name": [
            "The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Carmeliet", 
        "givenName": "Peter", 
        "id": "sg:person.01033041553.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033041553.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 de Caen Basse-Normandie", 
          "id": "https://www.grid.ac/institutes/grid.412043.0", 
          "name": [
            "Universit\u00e9 de CAEN, UMR CNRS 6551, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN, Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "MacKenzie", 
        "givenName": "Eric T.", 
        "id": "sg:person.01116522214.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116522214.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 de Caen Basse-Normandie", 
          "id": "https://www.grid.ac/institutes/grid.412043.0", 
          "name": [
            "Universit\u00e9 de CAEN, UMR CNRS 6551, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN, Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vivien", 
        "givenName": "Denis", 
        "id": "sg:person.0773120341.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773120341.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 de Caen Basse-Normandie", 
          "id": "https://www.grid.ac/institutes/grid.412043.0", 
          "name": [
            "Universit\u00e9 de CAEN, UMR CNRS 6551, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN, Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Buisson", 
        "givenName": "Alain", 
        "id": "sg:person.01006607040.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006607040.82"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0960-9822(98)70016-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004107343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.17.7627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004428940"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)80597-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004802940"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/19819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007133701", 
          "https://doi.org/10.1038/19819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199512143332401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008672430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-461201-3.50010-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010430710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1471-4159.1998.71062349.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013701096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0298-228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013754624", 
          "https://doi.org/10.1038/nm0298-228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0298-228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013754624", 
          "https://doi.org/10.1038/nm0298-228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(88)90162-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015528818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/399a007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018898529", 
          "https://doi.org/10.1038/399a007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/399a007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018898529", 
          "https://doi.org/10.1038/399a007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.19.12662", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019544574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/377340a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032270773", 
          "https://doi.org/10.1038/377340a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1432-1327.1999.00545.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039476094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-3806(94)90066-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043811872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-3806(94)90066-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043811872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci116637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044420168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/368419a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046408145", 
          "https://doi.org/10.1038/368419a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-0270(87)90041-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046423432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-0270(87)90041-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046423432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/307462a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047554393", 
          "https://doi.org/10.1038/307462a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0028-3908(95)00109-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051808483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.12.6031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051931812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.3.1976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053482394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.284.5414.647", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062565022"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.19-16-06723.1999", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074502494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081750825", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.16-07-02220.1996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082876221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpheart.1997.273.5.h2091", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083174289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fasebj.12.15.1683", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083355805"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2001-01", 
    "datePublishedReg": "2001-01-01", 
    "description": "Tissue-plasminogen activator (t-PA) is now available for the treatment of thrombo-embolic stroke but adverse effects have been reported in some patients, particularly hemorrhaging. In contrast, the results of animal studies have indicated that t-PA could increase neuronal damage after focal cerebral ischemia. Here we report for the first time that t-PA potentiates signaling mediated by glutamatergic receptors by modifying the properties of the N-methyl-D-aspartate (NMDA) receptor. When depolarized, cortical neurons release bio-active t-PA that interacts with and cleaves the NR1 subunit of the NMDA receptor. Moreover, the treatment with recombinant t-PA leads to a 37% increase in NMDA-stimulated fura-2 fluorescence, which may reflect an increased NMDA-receptor function. These results were confirmed in vivo by the intrastriatal injection of recombinant-PA, which potentiated the excitotoxic lesions induced by NMDA. These data provide insight into the regulation of NMDA-receptor-mediated signaling and could initiate therapeutic strategies to improve the efficacy of t-PA treatment in man.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/83358", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1113716", 
        "issn": [
          "1078-8956", 
          "1546-170X"
        ], 
        "name": "Nature Medicine", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "The proteolytic activity of tissue-plasminogen activator enhances NMDA receptor-mediated signaling", 
    "pagination": "nm0101_59", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e52874170bc0e36f8ee1d0ea35e22f7dd71f71a5ff6aecc223991fddd5a351f0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11135617"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9502015"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/83358"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1048103862"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/83358", 
      "https://app.dimensions.ai/details/publication/pub.1048103862"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T00:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8695_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/nm0101_59"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/83358'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/83358'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/83358'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/83358'


 

This table displays all metadata directly associated to this object as RDF triples.

251 TRIPLES      21 PREDICATES      66 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/83358 schema:about N1982f6fc0e7948069ed35dfa1490619e
2 N57ce03afe42c4a288d01cfd834218f9e
3 N6285270d70bf4d48b636f99f364d66eb
4 Nc56f263f91774a4f84db74b888bf9ef0
5 Nc773ffd443ee4824997ce8f09d272067
6 Ncb8fca2f89454e5a829ca150ac22a39e
7 Nd2c4fa6e14e24b93b76ded401f68bd57
8 Ndf80ea23151b4c24b0838670e19c419d
9 Ne561cb5ccff74c6fa06b88764764d7d8
10 Ne6d3094f727d41d29f1261507e178590
11 anzsrc-for:11
12 anzsrc-for:1109
13 schema:author N6cd0126f0a4a426d945ac5e625bcb996
14 schema:citation sg:pub.10.1038/19819
15 sg:pub.10.1038/307462a0
16 sg:pub.10.1038/368419a0
17 sg:pub.10.1038/377340a0
18 sg:pub.10.1038/399a007
19 sg:pub.10.1038/nm0298-228
20 https://app.dimensions.ai/details/publication/pub.1081750825
21 https://doi.org/10.1016/0028-3908(95)00109-j
22 https://doi.org/10.1016/0165-0270(87)90041-0
23 https://doi.org/10.1016/0165-3806(94)90066-3
24 https://doi.org/10.1016/0896-6273(88)90162-6
25 https://doi.org/10.1016/b978-0-12-461201-3.50010-4
26 https://doi.org/10.1016/s0896-6273(00)80597-8
27 https://doi.org/10.1016/s0960-9822(98)70016-8
28 https://doi.org/10.1046/j.1432-1327.1999.00545.x
29 https://doi.org/10.1046/j.1471-4159.1998.71062349.x
30 https://doi.org/10.1056/nejm199512143332401
31 https://doi.org/10.1073/pnas.92.17.7627
32 https://doi.org/10.1073/pnas.93.12.6031
33 https://doi.org/10.1074/jbc.272.19.12662
34 https://doi.org/10.1074/jbc.272.3.1976
35 https://doi.org/10.1096/fasebj.12.15.1683
36 https://doi.org/10.1126/science.284.5414.647
37 https://doi.org/10.1152/ajpheart.1997.273.5.h2091
38 https://doi.org/10.1172/jci116637
39 https://doi.org/10.1523/jneurosci.16-07-02220.1996
40 https://doi.org/10.1523/jneurosci.19-16-06723.1999
41 schema:datePublished 2001-01
42 schema:datePublishedReg 2001-01-01
43 schema:description Tissue-plasminogen activator (t-PA) is now available for the treatment of thrombo-embolic stroke but adverse effects have been reported in some patients, particularly hemorrhaging. In contrast, the results of animal studies have indicated that t-PA could increase neuronal damage after focal cerebral ischemia. Here we report for the first time that t-PA potentiates signaling mediated by glutamatergic receptors by modifying the properties of the N-methyl-D-aspartate (NMDA) receptor. When depolarized, cortical neurons release bio-active t-PA that interacts with and cleaves the NR1 subunit of the NMDA receptor. Moreover, the treatment with recombinant t-PA leads to a 37% increase in NMDA-stimulated fura-2 fluorescence, which may reflect an increased NMDA-receptor function. These results were confirmed in vivo by the intrastriatal injection of recombinant-PA, which potentiated the excitotoxic lesions induced by NMDA. These data provide insight into the regulation of NMDA-receptor-mediated signaling and could initiate therapeutic strategies to improve the efficacy of t-PA treatment in man.
44 schema:genre research_article
45 schema:inLanguage en
46 schema:isAccessibleForFree false
47 schema:isPartOf N62a4b92d64a941f394add1b6f36bbb53
48 N988f0dde4eba4f8b90cbcb101c2a9ff6
49 sg:journal.1113716
50 schema:name The proteolytic activity of tissue-plasminogen activator enhances NMDA receptor-mediated signaling
51 schema:pagination nm0101_59
52 schema:productId N063225542ca7451b9f4197dd6ebdf704
53 N1d85b5d8bce94dd79cd88416afe3aa86
54 N89a0d4d37179481c9acd35e578d4c5bb
55 Nb6829755d4e04f659de36c4ba6b409a0
56 Nef6f986138de49c68cc354fc740d6b79
57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048103862
58 https://doi.org/10.1038/83358
59 schema:sdDatePublished 2019-04-11T00:04
60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
61 schema:sdPublisher Nfbd46cac93114a9795bafbf3ee501a93
62 schema:url http://www.nature.com/articles/nm0101_59
63 sgo:license sg:explorer/license/
64 sgo:sdDataset articles
65 rdf:type schema:ScholarlyArticle
66 N063225542ca7451b9f4197dd6ebdf704 schema:name doi
67 schema:value 10.1038/83358
68 rdf:type schema:PropertyValue
69 N184d567d5e6a4869ac56d0dc46c2ecb3 rdf:first sg:person.0773120341.98
70 rdf:rest N40793a9fd2b5400d98c07c3cc0bca2c5
71 N1982f6fc0e7948069ed35dfa1490619e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Hydrolysis
73 rdf:type schema:DefinedTerm
74 N1d85b5d8bce94dd79cd88416afe3aa86 schema:name dimensions_id
75 schema:value pub.1048103862
76 rdf:type schema:PropertyValue
77 N40793a9fd2b5400d98c07c3cc0bca2c5 rdf:first sg:person.01006607040.82
78 rdf:rest rdf:nil
79 N57ce03afe42c4a288d01cfd834218f9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Cell Death
81 rdf:type schema:DefinedTerm
82 N6285270d70bf4d48b636f99f364d66eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Ion Transport
84 rdf:type schema:DefinedTerm
85 N62a4b92d64a941f394add1b6f36bbb53 schema:volumeNumber 7
86 rdf:type schema:PublicationVolume
87 N6cd0126f0a4a426d945ac5e625bcb996 rdf:first sg:person.01117174340.95
88 rdf:rest Nd7cf66b1cf0047b38042ab2103e76e89
89 N84be700670fa48148fd0d62e3529e6e5 rdf:first sg:person.01116522214.96
90 rdf:rest N184d567d5e6a4869ac56d0dc46c2ecb3
91 N89a0d4d37179481c9acd35e578d4c5bb schema:name nlm_unique_id
92 schema:value 9502015
93 rdf:type schema:PropertyValue
94 N988f0dde4eba4f8b90cbcb101c2a9ff6 schema:issueNumber 1
95 rdf:type schema:PublicationIssue
96 Nb6829755d4e04f659de36c4ba6b409a0 schema:name readcube_id
97 schema:value e52874170bc0e36f8ee1d0ea35e22f7dd71f71a5ff6aecc223991fddd5a351f0
98 rdf:type schema:PropertyValue
99 Nc56f263f91774a4f84db74b888bf9ef0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Signal Transduction
101 rdf:type schema:DefinedTerm
102 Nc773ffd443ee4824997ce8f09d272067 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Calcium
104 rdf:type schema:DefinedTerm
105 Ncb8fca2f89454e5a829ca150ac22a39e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Animals
107 rdf:type schema:DefinedTerm
108 Nd2c4fa6e14e24b93b76ded401f68bd57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Receptors, N-Methyl-D-Aspartate
110 rdf:type schema:DefinedTerm
111 Nd438a85050f04ff18cdcf25d259bb6c5 rdf:first sg:person.01235307632.57
112 rdf:rest Nef943edc6091477aadad88c7c889e2a8
113 Nd7cf66b1cf0047b38042ab2103e76e89 rdf:first sg:person.01125221124.20
114 rdf:rest Nd438a85050f04ff18cdcf25d259bb6c5
115 Ndf80ea23151b4c24b0838670e19c419d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Tissue Plasminogen Activator
117 rdf:type schema:DefinedTerm
118 Ne561cb5ccff74c6fa06b88764764d7d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Neurons
120 rdf:type schema:DefinedTerm
121 Ne6d3094f727d41d29f1261507e178590 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Membrane Potentials
123 rdf:type schema:DefinedTerm
124 Ne7635311d0d94031a188128bb97f1399 rdf:first sg:person.01033041553.19
125 rdf:rest N84be700670fa48148fd0d62e3529e6e5
126 Nef6f986138de49c68cc354fc740d6b79 schema:name pubmed_id
127 schema:value 11135617
128 rdf:type schema:PropertyValue
129 Nef943edc6091477aadad88c7c889e2a8 rdf:first sg:person.01065077153.51
130 rdf:rest Ne7635311d0d94031a188128bb97f1399
131 Nfbd46cac93114a9795bafbf3ee501a93 schema:name Springer Nature - SN SciGraph project
132 rdf:type schema:Organization
133 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
134 schema:name Medical and Health Sciences
135 rdf:type schema:DefinedTerm
136 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
137 schema:name Neurosciences
138 rdf:type schema:DefinedTerm
139 sg:journal.1113716 schema:issn 1078-8956
140 1546-170X
141 schema:name Nature Medicine
142 rdf:type schema:Periodical
143 sg:person.01006607040.82 schema:affiliation https://www.grid.ac/institutes/grid.412043.0
144 schema:familyName Buisson
145 schema:givenName Alain
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006607040.82
147 rdf:type schema:Person
148 sg:person.01033041553.19 schema:affiliation https://www.grid.ac/institutes/grid.11486.3a
149 schema:familyName Carmeliet
150 schema:givenName Peter
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033041553.19
152 rdf:type schema:Person
153 sg:person.01065077153.51 schema:affiliation https://www.grid.ac/institutes/grid.10992.33
154 schema:familyName Margaill
155 schema:givenName Isabelle
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065077153.51
157 rdf:type schema:Person
158 sg:person.01116522214.96 schema:affiliation https://www.grid.ac/institutes/grid.412043.0
159 schema:familyName MacKenzie
160 schema:givenName Eric T.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116522214.96
162 rdf:type schema:Person
163 sg:person.01117174340.95 schema:affiliation https://www.grid.ac/institutes/grid.412043.0
164 schema:familyName Nicole
165 schema:givenName Olivier
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117174340.95
167 rdf:type schema:Person
168 sg:person.01125221124.20 schema:affiliation https://www.grid.ac/institutes/grid.412043.0
169 schema:familyName Docagne
170 schema:givenName Fabian
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125221124.20
172 rdf:type schema:Person
173 sg:person.01235307632.57 schema:affiliation https://www.grid.ac/institutes/grid.412043.0
174 schema:familyName Ali
175 schema:givenName Carine
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235307632.57
177 rdf:type schema:Person
178 sg:person.0773120341.98 schema:affiliation https://www.grid.ac/institutes/grid.412043.0
179 schema:familyName Vivien
180 schema:givenName Denis
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773120341.98
182 rdf:type schema:Person
183 sg:pub.10.1038/19819 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007133701
184 https://doi.org/10.1038/19819
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/307462a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047554393
187 https://doi.org/10.1038/307462a0
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/368419a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046408145
190 https://doi.org/10.1038/368419a0
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/377340a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032270773
193 https://doi.org/10.1038/377340a0
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/399a007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018898529
196 https://doi.org/10.1038/399a007
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/nm0298-228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013754624
199 https://doi.org/10.1038/nm0298-228
200 rdf:type schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1081750825 schema:CreativeWork
202 https://doi.org/10.1016/0028-3908(95)00109-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1051808483
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0165-0270(87)90041-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046423432
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0165-3806(94)90066-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043811872
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/0896-6273(88)90162-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015528818
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/b978-0-12-461201-3.50010-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010430710
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/s0896-6273(00)80597-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004802940
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/s0960-9822(98)70016-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004107343
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1046/j.1432-1327.1999.00545.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1039476094
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1046/j.1471-4159.1998.71062349.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013701096
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1056/nejm199512143332401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008672430
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1073/pnas.92.17.7627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004428940
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1073/pnas.93.12.6031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051931812
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1074/jbc.272.19.12662 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019544574
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1074/jbc.272.3.1976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053482394
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1096/fasebj.12.15.1683 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083355805
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1126/science.284.5414.647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062565022
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1152/ajpheart.1997.273.5.h2091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083174289
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1172/jci116637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044420168
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1523/jneurosci.16-07-02220.1996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082876221
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1523/jneurosci.19-16-06723.1999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074502494
241 rdf:type schema:CreativeWork
242 https://www.grid.ac/institutes/grid.10992.33 schema:alternateName Paris Descartes University
243 schema:name Laboratoire de pharmacologie, Faculté des Sciences Pharmaceutiques et Biologiques, Université René Descartes, Paris F-75006, France
244 rdf:type schema:Organization
245 https://www.grid.ac/institutes/grid.11486.3a schema:alternateName Flanders Institute for Biotechnology
246 schema:name The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium
247 rdf:type schema:Organization
248 https://www.grid.ac/institutes/grid.412043.0 schema:alternateName Université de Caen Basse-Normandie
249 schema:name CEA-Université LRA10V, UPRES EA 2609, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN Cedex, France
250 Université de CAEN, UMR CNRS 6551, Bd H. Becquerel, Cyeron BP 5229, F-14074 CAEN, Cedex, France
251 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...