Antagonism of Rho-kinase stimulates rat penile erection via a nitric oxide-independent pathway View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-01

AUTHORS

Kanchan Chitaley, Christopher J. Wingard, R. Clinton Webb, Heather Branam, Vivienne S. Stopper, Ronald W. Lewis, Thomas M. Mills

ABSTRACT

Relaxation of the smooth muscle cells in the cavernosal arterioles and sinuses results in increased blood flow into the penis, raising corpus cavernosum pressure to culminate in penile erection. Nitric oxide, released from non-adrenergic/non-cholinergic nerves, is considered the principle stimulator of cavernosal smooth muscle relaxation, however, the inhibition of vasoconstrictors (that is, norepinephrine and endothelin-1, refs. 5-9) cannot be ignored as a potential regulator of penile erection. The calcium-sensitizing rho-A/Rho-kinase pathway may play a synergistic role in cavernosal vasoconstriction to maintain penile flaccidity. Rho-kinase is known to inhibit myosin light chain phosphatase, and to directly phosphorylate myosin light-chain (in solution), altogether resulting in a net increase in activated myosin and the promotion of cellular contraction. Although Rho-kinase protein and mRNA have been detected in cavernosal tissue, the role of Rho-kinase in the regulation of cavernosal tone is unknown. Using pharmacologic antagonism (Y-27632, ref. 13, 18), we examined the role of Rho-kinase in cavernosal tone, based on the hypothesis that antagonism of Rho-kinase results in increased corpus cavernosum pressure, initiating the erectile response independently of nitric oxide. Our finding, that Rho-kinase antagonism stimulates rat penile erection independently of nitric oxide, introduces a potential alternate avenue for the treatment of erectile dysfunction. More... »

PAGES

nm0101_119

Journal

TITLE

Nature Medicine

ISSUE

1

VOLUME

7

Related Patents

  • Anti-Inflammatory And Protein Kinase Inhibitor Compositions And Related Methods For Downregulation Of Detrimental Cellular Responses And Inhibition Of Cell Death
  • Pyridazine Compounds, Compositions And Methods
  • Cycloalkylamine Substituted Isoquinoline And Isoquinolinone Derivatives
  • Treatment Of Mild And Moderate Alzheimer's Disease
  • Isoquinoline Derivatives
  • Pyridazine Compounds, Compositions And Methods
  • Substituted Imidazole Derivatives And Methods Of Use Thereof
  • 6-Substituted Isoquinolines And Isoquinolinones
  • Inhibitors Of Influenza Viruses Replication
  • Inhibitors Of Influenza Viruses Replication
  • Azole Derivatives And Fused Bicyclic Azole Derivatives As Therapeutic Agents
  • Cyclohexylamin Isoquinolone Derivatives
  • Anti-Inflammatory And Protein Kinase Inhibitor Compositions And Related Methods For Downregulation Of Detrimental Cellular Responses And Inhibition Of Cell Death
  • Substituted Isoquinoline And Isoquinolinone Derivatives
  • Benzimidazole Derivatives As Therapeutic Agents
  • Inhibitors Of Influenza Viruses Replication
  • Isoquinoline Derivatives
  • Method For The Synthesis Of Compounds Of Formula I And Their Uses Thereof
  • Piperidinyl-Substituted Isoquinolone Derivatives
  • Formulations Containing Pyridazine Compounds
  • Cycloalkylamine Substituted Isoquinolone Derivatives
  • Bi- And Polycyclic Substituted Isoquinoline And Isoquinolinone Derivatives
  • Carboxamide Derivatives As Therapeutic Agents
  • 4-(4-Pyridinyl)-Benzamides And Their Use As Rock Activity Modulators
  • Inhibitors Of Influenza Viruses Replication
  • Method For The Synthesis Of Compounds Of Formula I And Their Uses Thereof
  • Mono- And Bicyclic Azole Derivatives That Inhibit The Interaction Of Ligands With Rage
  • 4-(4-Pyridinyl)-Benzamides And Their Use As Rock Activity Modulators
  • Substituted Isoquinoline And Isoquinolinone Derivatives
  • Benzimidazole Derivatives As Therapeutic Agents
  • Carboxamide Derivatives As Therapeutic Agents
  • Inhibitors Of Influenza Viruses Replication
  • Cycloalkylamine Substituted Isoquinoline Derivatives
  • Substituted Isoquinoline And Isoquinolinone Derivatives
  • Inhibitors Of Influenza Viruses Replication
  • Azole Derivatives And Fused Bicyclic Azole Derivatives As Therapeutic Agents
  • Azole Derivatives And Fused Bicyclic Azole Derivatives As Therapeutic Agents
  • Inhibitors Of Influenza Viruses Replication
  • Pyridazine Compounds And Methods
  • Methods To Identify Compounds That Modulate Rage
  • Methods Of Preparing Inhibitors Of Influenza Viruses Replication
  • Pyridazine Compounds, Compositions And Methods
  • Phenyl-Heteroaryl Derivatives And Methods Of Use Thereof
  • Substituted Isoquinoline And Isoquinolinone Derivatives As Inhibitors Of Rho-Kinase
  • Pyridazine Compounds, Compositions And Methods
  • Compositions And Treatments Using Pyridazine Compounds And Cholinesterase Inhibitors
  • Substituted Isoquinolines And Isoquinolinones As Rho Kinase Inhibitors
  • Inhibitors Of Influenza Viruses Replication
  • Methods To Identify Compounds That Modulate Rage
  • Phenyl-Heteroaryl Derivatives And Methods Of Use Thereof
  • Cyclohexylamine Isoquinolone Derivatives
  • Inhibitors Of Influenza Viruses Replication
  • Phenyl-Heteroaryl Derivatives And Methods Of Use Thereof
  • Inhibitors Of Influenza Viruses Replication
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/83258

    DOI

    http://dx.doi.org/10.1038/83258

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1036056981

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11135626


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1116", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Physiology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Western", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Enzyme Inhibitors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nitric Oxide", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Penile Erection", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Kinase Inhibitors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Kinases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Messenger", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Rats", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Rats, Sprague-Dawley", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Michigan\u2013Ann Arbor", 
              "id": "https://www.grid.ac/institutes/grid.214458.e", 
              "name": [
                "Department of Physiology, University of Michigan, Ann Arbor, Michigan 48109, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chitaley", 
            "givenName": "Kanchan", 
            "id": "sg:person.01327473426.71", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327473426.71"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Augusta University", 
              "id": "https://www.grid.ac/institutes/grid.410427.4", 
              "name": [
                "Department of Physiology, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wingard", 
            "givenName": "Christopher J.", 
            "id": "sg:person.01040444450.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040444450.77"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Augusta University", 
              "id": "https://www.grid.ac/institutes/grid.410427.4", 
              "name": [
                "Department of Physiology, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Webb", 
            "givenName": "R. Clinton", 
            "id": "sg:person.0600774175.33", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600774175.33"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Augusta University", 
              "id": "https://www.grid.ac/institutes/grid.410427.4", 
              "name": [
                "Department of Physiology, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Branam", 
            "givenName": "Heather", 
            "id": "sg:person.01330175767.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330175767.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Augusta University", 
              "id": "https://www.grid.ac/institutes/grid.410427.4", 
              "name": [
                "Department of Physiology, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stopper", 
            "givenName": "Vivienne S.", 
            "id": "sg:person.01254273162.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254273162.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Augusta University", 
              "id": "https://www.grid.ac/institutes/grid.410427.4", 
              "name": [
                "Department of Surgery, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lewis", 
            "givenName": "Ronald W.", 
            "id": "sg:person.013617142672.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013617142672.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Augusta University", 
              "id": "https://www.grid.ac/institutes/grid.410427.4", 
              "name": [
                "Department of Physiology, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA", 
                "Department of Surgery, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mills", 
            "givenName": "Thomas M.", 
            "id": "sg:person.016605560712.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016605560712.63"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf01275151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001151020", 
              "https://doi.org/10.1007/bf01275151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01275151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001151020", 
              "https://doi.org/10.1007/bf01275151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1469-7793.2000.t01-2-00177.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005913479"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-5347(01)64368-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007094018"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1469-7793.1999.261aa.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008960783"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-5347(01)62432-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009898018"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1939-4640.1997.tb02435.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012637949"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/40187", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014459412", 
              "https://doi.org/10.1038/40187"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/40187", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014459412", 
              "https://doi.org/10.1038/40187"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1939-4640.1997.tb01890.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014485356"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/humupd/4.1.3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017223223"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1159/000056123", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018573904"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0090-4295(99)00310-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019950730"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.272.19.12257", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022491866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199909010-00024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023251806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199909010-00024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023251806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1095/biolreprod59.6.1413", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024311281"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1095/biolreprod59.6.1413", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024311281"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.274.6.3744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024342815"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-3495(00)76754-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025296010"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.271.34.20246", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030487068"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-201x.1998.00454.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040635592"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.94.15.8226", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047368423"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-2999(96)00172-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049722442"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199709000-00076", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060214273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199709000-00076", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060214273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199709000-00076", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060214273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199811000-00072", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060215799"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199811000-00072", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060215799"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00005392-199811000-00072", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060215799"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.273.5272.245", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062553597"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1358/dot.2000.36.2-3.568787", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1065063258"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074628759", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076750128", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2001-01", 
        "datePublishedReg": "2001-01-01", 
        "description": "Relaxation of the smooth muscle cells in the cavernosal arterioles and sinuses results in increased blood flow into the penis, raising corpus cavernosum pressure to culminate in penile erection. Nitric oxide, released from non-adrenergic/non-cholinergic nerves, is considered the principle stimulator of cavernosal smooth muscle relaxation, however, the inhibition of vasoconstrictors (that is, norepinephrine and endothelin-1, refs. 5-9) cannot be ignored as a potential regulator of penile erection. The calcium-sensitizing rho-A/Rho-kinase pathway may play a synergistic role in cavernosal vasoconstriction to maintain penile flaccidity. Rho-kinase is known to inhibit myosin light chain phosphatase, and to directly phosphorylate myosin light-chain (in solution), altogether resulting in a net increase in activated myosin and the promotion of cellular contraction. Although Rho-kinase protein and mRNA have been detected in cavernosal tissue, the role of Rho-kinase in the regulation of cavernosal tone is unknown. Using pharmacologic antagonism (Y-27632, ref. 13, 18), we examined the role of Rho-kinase in cavernosal tone, based on the hypothesis that antagonism of Rho-kinase results in increased corpus cavernosum pressure, initiating the erectile response independently of nitric oxide. Our finding, that Rho-kinase antagonism stimulates rat penile erection independently of nitric oxide, introduces a potential alternate avenue for the treatment of erectile dysfunction.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/83258", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2683907", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2436718", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1113716", 
            "issn": [
              "1078-8956", 
              "1546-170X"
            ], 
            "name": "Nature Medicine", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "name": "Antagonism of Rho-kinase stimulates rat penile erection via a nitric oxide-independent pathway", 
        "pagination": "nm0101_119", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "abc2e719ec7a482c306ad443550ef33708dc328395b79ea101843faaed42b3b2"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "11135626"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9502015"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/83258"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1036056981"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/83258", 
          "https://app.dimensions.ai/details/publication/pub.1036056981"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T18:56", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000425.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nm0101_119"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/83258'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/83258'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/83258'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/83258'


     

    This table displays all metadata directly associated to this object as RDF triples.

    241 TRIPLES      21 PREDICATES      66 URIs      32 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/83258 schema:about N27fb1fc99a0f45e692d62d85075dccf3
    2 N737fb7fd8fd34b18aa61c84666341035
    3 N74e7f4a8d15f4ceab92a909e3c4616ae
    4 N7d947d4d2f7040cd8d354f35851585a4
    5 Nb6b29c48ddb24d0ebc02d0de80cfcb8f
    6 Nbff9f7b668764e09b4d142934deccc42
    7 Ncb4e396748a94a3dac981e2865d33d76
    8 Nd6542ef35988482ca4dc3a9d952c6999
    9 Ndde1dc6de8114bef99980fe07a0720cc
    10 Ne13793b8e6594fbdbf580e9f3906ff50
    11 Nfc428a373384430c8d0b5d4c6fd4dbe3
    12 anzsrc-for:11
    13 anzsrc-for:1116
    14 schema:author N69c3398a2f6f467ba17924188ca10ba3
    15 schema:citation sg:pub.10.1007/bf01275151
    16 sg:pub.10.1038/40187
    17 https://app.dimensions.ai/details/publication/pub.1074628759
    18 https://app.dimensions.ai/details/publication/pub.1076750128
    19 https://doi.org/10.1002/j.1939-4640.1997.tb01890.x
    20 https://doi.org/10.1002/j.1939-4640.1997.tb02435.x
    21 https://doi.org/10.1016/0014-2999(96)00172-0
    22 https://doi.org/10.1016/s0006-3495(00)76754-3
    23 https://doi.org/10.1016/s0022-5347(01)62432-7
    24 https://doi.org/10.1016/s0022-5347(01)64368-4
    25 https://doi.org/10.1016/s0090-4295(99)00310-6
    26 https://doi.org/10.1046/j.1365-201x.1998.00454.x
    27 https://doi.org/10.1073/pnas.94.15.8226
    28 https://doi.org/10.1074/jbc.271.34.20246
    29 https://doi.org/10.1074/jbc.272.19.12257
    30 https://doi.org/10.1074/jbc.274.6.3744
    31 https://doi.org/10.1093/humupd/4.1.3
    32 https://doi.org/10.1095/biolreprod59.6.1413
    33 https://doi.org/10.1097/00005392-199709000-00076
    34 https://doi.org/10.1097/00005392-199811000-00072
    35 https://doi.org/10.1097/00005392-199909010-00024
    36 https://doi.org/10.1111/j.1469-7793.1999.261aa.x
    37 https://doi.org/10.1111/j.1469-7793.2000.t01-2-00177.x
    38 https://doi.org/10.1126/science.273.5272.245
    39 https://doi.org/10.1159/000056123
    40 https://doi.org/10.1358/dot.2000.36.2-3.568787
    41 schema:datePublished 2001-01
    42 schema:datePublishedReg 2001-01-01
    43 schema:description Relaxation of the smooth muscle cells in the cavernosal arterioles and sinuses results in increased blood flow into the penis, raising corpus cavernosum pressure to culminate in penile erection. Nitric oxide, released from non-adrenergic/non-cholinergic nerves, is considered the principle stimulator of cavernosal smooth muscle relaxation, however, the inhibition of vasoconstrictors (that is, norepinephrine and endothelin-1, refs. 5-9) cannot be ignored as a potential regulator of penile erection. The calcium-sensitizing rho-A/Rho-kinase pathway may play a synergistic role in cavernosal vasoconstriction to maintain penile flaccidity. Rho-kinase is known to inhibit myosin light chain phosphatase, and to directly phosphorylate myosin light-chain (in solution), altogether resulting in a net increase in activated myosin and the promotion of cellular contraction. Although Rho-kinase protein and mRNA have been detected in cavernosal tissue, the role of Rho-kinase in the regulation of cavernosal tone is unknown. Using pharmacologic antagonism (Y-27632, ref. 13, 18), we examined the role of Rho-kinase in cavernosal tone, based on the hypothesis that antagonism of Rho-kinase results in increased corpus cavernosum pressure, initiating the erectile response independently of nitric oxide. Our finding, that Rho-kinase antagonism stimulates rat penile erection independently of nitric oxide, introduces a potential alternate avenue for the treatment of erectile dysfunction.
    44 schema:genre research_article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree false
    47 schema:isPartOf N217e774cc1a74ed8a80be56b62300997
    48 N52ab9d37a9914e51bfd6b06deacc3058
    49 sg:journal.1113716
    50 schema:name Antagonism of Rho-kinase stimulates rat penile erection via a nitric oxide-independent pathway
    51 schema:pagination nm0101_119
    52 schema:productId N35300203927241a8bb7b6f70c0d1bb64
    53 N3a24831f34bd484988ccc780d234a789
    54 N6d70da3f06ac42ebb4b90c19a2ea834a
    55 Need33623d0ce4795be41c8181120bf64
    56 Nf76ceeb7fbd3467595c28a2f5af0e7df
    57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036056981
    58 https://doi.org/10.1038/83258
    59 schema:sdDatePublished 2019-04-10T18:56
    60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    61 schema:sdPublisher Ne67457440ca04d6f8c23cad8713d0b36
    62 schema:url http://www.nature.com/articles/nm0101_119
    63 sgo:license sg:explorer/license/
    64 sgo:sdDataset articles
    65 rdf:type schema:ScholarlyArticle
    66 N217e774cc1a74ed8a80be56b62300997 schema:volumeNumber 7
    67 rdf:type schema:PublicationVolume
    68 N27fb1fc99a0f45e692d62d85075dccf3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    69 schema:name Animals
    70 rdf:type schema:DefinedTerm
    71 N29830ddf9b5546939a55c07ae5d62a59 rdf:first sg:person.0600774175.33
    72 rdf:rest N504166a0b4d84175a498d9a4e2739bef
    73 N35300203927241a8bb7b6f70c0d1bb64 schema:name dimensions_id
    74 schema:value pub.1036056981
    75 rdf:type schema:PropertyValue
    76 N3a24831f34bd484988ccc780d234a789 schema:name doi
    77 schema:value 10.1038/83258
    78 rdf:type schema:PropertyValue
    79 N504166a0b4d84175a498d9a4e2739bef rdf:first sg:person.01330175767.20
    80 rdf:rest N538953e218584948a441af9763ca5295
    81 N52ab9d37a9914e51bfd6b06deacc3058 schema:issueNumber 1
    82 rdf:type schema:PublicationIssue
    83 N538953e218584948a441af9763ca5295 rdf:first sg:person.01254273162.57
    84 rdf:rest N586250bdbc604816b7bf960b9851f1d3
    85 N586250bdbc604816b7bf960b9851f1d3 rdf:first sg:person.013617142672.04
    86 rdf:rest Ne4958e72fada40a18bb7c7b52f82f34a
    87 N69c3398a2f6f467ba17924188ca10ba3 rdf:first sg:person.01327473426.71
    88 rdf:rest Na20f154f857c4fc680295ac429d75095
    89 N6d70da3f06ac42ebb4b90c19a2ea834a schema:name pubmed_id
    90 schema:value 11135626
    91 rdf:type schema:PropertyValue
    92 N737fb7fd8fd34b18aa61c84666341035 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Enzyme Inhibitors
    94 rdf:type schema:DefinedTerm
    95 N74e7f4a8d15f4ceab92a909e3c4616ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Blotting, Western
    97 rdf:type schema:DefinedTerm
    98 N7d947d4d2f7040cd8d354f35851585a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Protein Kinase Inhibitors
    100 rdf:type schema:DefinedTerm
    101 Na20f154f857c4fc680295ac429d75095 rdf:first sg:person.01040444450.77
    102 rdf:rest N29830ddf9b5546939a55c07ae5d62a59
    103 Nb6b29c48ddb24d0ebc02d0de80cfcb8f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name RNA, Messenger
    105 rdf:type schema:DefinedTerm
    106 Nbff9f7b668764e09b4d142934deccc42 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Male
    108 rdf:type schema:DefinedTerm
    109 Ncb4e396748a94a3dac981e2865d33d76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Rats
    111 rdf:type schema:DefinedTerm
    112 Nd6542ef35988482ca4dc3a9d952c6999 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Penile Erection
    114 rdf:type schema:DefinedTerm
    115 Ndde1dc6de8114bef99980fe07a0720cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Nitric Oxide
    117 rdf:type schema:DefinedTerm
    118 Ne13793b8e6594fbdbf580e9f3906ff50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Protein Kinases
    120 rdf:type schema:DefinedTerm
    121 Ne4958e72fada40a18bb7c7b52f82f34a rdf:first sg:person.016605560712.63
    122 rdf:rest rdf:nil
    123 Ne67457440ca04d6f8c23cad8713d0b36 schema:name Springer Nature - SN SciGraph project
    124 rdf:type schema:Organization
    125 Need33623d0ce4795be41c8181120bf64 schema:name readcube_id
    126 schema:value abc2e719ec7a482c306ad443550ef33708dc328395b79ea101843faaed42b3b2
    127 rdf:type schema:PropertyValue
    128 Nf76ceeb7fbd3467595c28a2f5af0e7df schema:name nlm_unique_id
    129 schema:value 9502015
    130 rdf:type schema:PropertyValue
    131 Nfc428a373384430c8d0b5d4c6fd4dbe3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Rats, Sprague-Dawley
    133 rdf:type schema:DefinedTerm
    134 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    135 schema:name Medical and Health Sciences
    136 rdf:type schema:DefinedTerm
    137 anzsrc-for:1116 schema:inDefinedTermSet anzsrc-for:
    138 schema:name Medical Physiology
    139 rdf:type schema:DefinedTerm
    140 sg:grant.2436718 http://pending.schema.org/fundedItem sg:pub.10.1038/83258
    141 rdf:type schema:MonetaryGrant
    142 sg:grant.2683907 http://pending.schema.org/fundedItem sg:pub.10.1038/83258
    143 rdf:type schema:MonetaryGrant
    144 sg:journal.1113716 schema:issn 1078-8956
    145 1546-170X
    146 schema:name Nature Medicine
    147 rdf:type schema:Periodical
    148 sg:person.01040444450.77 schema:affiliation https://www.grid.ac/institutes/grid.410427.4
    149 schema:familyName Wingard
    150 schema:givenName Christopher J.
    151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040444450.77
    152 rdf:type schema:Person
    153 sg:person.01254273162.57 schema:affiliation https://www.grid.ac/institutes/grid.410427.4
    154 schema:familyName Stopper
    155 schema:givenName Vivienne S.
    156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254273162.57
    157 rdf:type schema:Person
    158 sg:person.01327473426.71 schema:affiliation https://www.grid.ac/institutes/grid.214458.e
    159 schema:familyName Chitaley
    160 schema:givenName Kanchan
    161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327473426.71
    162 rdf:type schema:Person
    163 sg:person.01330175767.20 schema:affiliation https://www.grid.ac/institutes/grid.410427.4
    164 schema:familyName Branam
    165 schema:givenName Heather
    166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330175767.20
    167 rdf:type schema:Person
    168 sg:person.013617142672.04 schema:affiliation https://www.grid.ac/institutes/grid.410427.4
    169 schema:familyName Lewis
    170 schema:givenName Ronald W.
    171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013617142672.04
    172 rdf:type schema:Person
    173 sg:person.016605560712.63 schema:affiliation https://www.grid.ac/institutes/grid.410427.4
    174 schema:familyName Mills
    175 schema:givenName Thomas M.
    176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016605560712.63
    177 rdf:type schema:Person
    178 sg:person.0600774175.33 schema:affiliation https://www.grid.ac/institutes/grid.410427.4
    179 schema:familyName Webb
    180 schema:givenName R. Clinton
    181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600774175.33
    182 rdf:type schema:Person
    183 sg:pub.10.1007/bf01275151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001151020
    184 https://doi.org/10.1007/bf01275151
    185 rdf:type schema:CreativeWork
    186 sg:pub.10.1038/40187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014459412
    187 https://doi.org/10.1038/40187
    188 rdf:type schema:CreativeWork
    189 https://app.dimensions.ai/details/publication/pub.1074628759 schema:CreativeWork
    190 https://app.dimensions.ai/details/publication/pub.1076750128 schema:CreativeWork
    191 https://doi.org/10.1002/j.1939-4640.1997.tb01890.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014485356
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1002/j.1939-4640.1997.tb02435.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012637949
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1016/0014-2999(96)00172-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049722442
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1016/s0006-3495(00)76754-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025296010
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1016/s0022-5347(01)62432-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009898018
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1016/s0022-5347(01)64368-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007094018
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1016/s0090-4295(99)00310-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019950730
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1046/j.1365-201x.1998.00454.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040635592
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1073/pnas.94.15.8226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047368423
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1074/jbc.271.34.20246 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030487068
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1074/jbc.272.19.12257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022491866
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1074/jbc.274.6.3744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024342815
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1093/humupd/4.1.3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017223223
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1095/biolreprod59.6.1413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024311281
    218 rdf:type schema:CreativeWork
    219 https://doi.org/10.1097/00005392-199709000-00076 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060214273
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1097/00005392-199811000-00072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060215799
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.1097/00005392-199909010-00024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023251806
    224 rdf:type schema:CreativeWork
    225 https://doi.org/10.1111/j.1469-7793.1999.261aa.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008960783
    226 rdf:type schema:CreativeWork
    227 https://doi.org/10.1111/j.1469-7793.2000.t01-2-00177.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005913479
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1126/science.273.5272.245 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062553597
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1159/000056123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018573904
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1358/dot.2000.36.2-3.568787 schema:sameAs https://app.dimensions.ai/details/publication/pub.1065063258
    234 rdf:type schema:CreativeWork
    235 https://www.grid.ac/institutes/grid.214458.e schema:alternateName University of Michigan–Ann Arbor
    236 schema:name Department of Physiology, University of Michigan, Ann Arbor, Michigan 48109, USA
    237 rdf:type schema:Organization
    238 https://www.grid.ac/institutes/grid.410427.4 schema:alternateName Augusta University
    239 schema:name Department of Physiology, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA
    240 Department of Surgery, Urology Section, Medical College of Georgia, Augusta, Georgia 30912, USA
    241 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...