Structural proteomics of an archaeon View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-10-01

AUTHORS

Dinesh Christendat, Adelinda Yee, Akil Dharamsi, Yuval Kluger, Alexei Savchenko, John R. Cort, Valerie Booth, Cameron D. Mackereth, Vivian Saridakis, Irena Ekiel, Guennadi Kozlov, Karen L. Maxwell, Ning Wu, Lawrence P. McIntosh, Kalle Gehring, Michael A. Kennedy, Alan R. Davidson, Emil F. Pai, Mark Gerstein, Aled M. Edwards, Cheryl H. Arrowsmith

ABSTRACT

A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics. More... »

PAGES

nsb1000_903

References to SciGraph publications

  • 1999-03. Identification of the archaeal NMN adenylyltransferase gene in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 1998-12. 100,000 protein structures for the biologist in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/82823

    DOI

    http://dx.doi.org/10.1038/82823

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049408892

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11017201


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Crystallography, X-Ray", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Methanobacterium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Conformation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Proteome", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Christendat", 
            "givenName": "Dinesh", 
            "id": "sg:person.01347341027.78", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347341027.78"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yee", 
            "givenName": "Adelinda", 
            "id": "sg:person.01140615260.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140615260.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dharamsi", 
            "givenName": "Akil", 
            "id": "sg:person.01142325477.65", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142325477.65"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Yale University", 
              "id": "https://www.grid.ac/institutes/grid.47100.32", 
              "name": [
                "Department of Molecular Biophysics and Biochemistry and Computer Science, PO Box 208114, Yale University, New Haven , Connecticut 06520, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kluger", 
            "givenName": "Yuval", 
            "id": "sg:person.0731526665.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731526665.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Savchenko", 
            "givenName": "Alexei", 
            "id": "sg:person.015766703057.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015766703057.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pacific Northwest National Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.451303.0", 
              "name": [
                "Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratories, EMSL 2569 K8-98, Richland, Washington 99352, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cort", 
            "givenName": "John R.", 
            "id": "sg:person.0577535366.93", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577535366.93"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Booth", 
            "givenName": "Valerie", 
            "id": "sg:person.01261053663.24", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261053663.24"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of British Columbia", 
              "id": "https://www.grid.ac/institutes/grid.17091.3e", 
              "name": [
                "Departments of Biochemistry and Molecular Biology, Chemistry and the Biotechnology Laboratory, 2146 Health Sciences Mall, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mackereth", 
            "givenName": "Cameron D.", 
            "id": "sg:person.01301672667.11", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301672667.11"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Saridakis", 
            "givenName": "Vivian", 
            "id": "sg:person.0776013027.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776013027.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biotechnology Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.443931.8", 
              "name": [
                "Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Ave., Montreal , Quebec, Canada H4P 2R2."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ekiel", 
            "givenName": "Irena", 
            "id": "sg:person.0576253234.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576253234.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "McGill University", 
              "id": "https://www.grid.ac/institutes/grid.14709.3b", 
              "name": [
                "Department of Biochemistry and Montreal Joint Centre for Structural Biology, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada , H3G 1Y6."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kozlov", 
            "givenName": "Guennadi", 
            "id": "sg:person.0666544025.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666544025.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Maxwell", 
            "givenName": "Karen L.", 
            "id": "sg:person.01044552510.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044552510.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Ning", 
            "id": "sg:person.01152355061.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152355061.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of British Columbia", 
              "id": "https://www.grid.ac/institutes/grid.17091.3e", 
              "name": [
                "Departments of Biochemistry and Molecular Biology, Chemistry and the Biotechnology Laboratory, 2146 Health Sciences Mall, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3."
              ], 
              "type": "Organization"
            }, 
            "familyName": "McIntosh", 
            "givenName": "Lawrence P.", 
            "id": "sg:person.0657214023.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657214023.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "McGill University", 
              "id": "https://www.grid.ac/institutes/grid.14709.3b", 
              "name": [
                "Department of Biochemistry and Montreal Joint Centre for Structural Biology, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada , H3G 1Y6."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gehring", 
            "givenName": "Kalle", 
            "id": "sg:person.01324255516.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324255516.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pacific Northwest National Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.451303.0", 
              "name": [
                "Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratories, EMSL 2569 K8-98, Richland, Washington 99352, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kennedy", 
            "givenName": "Michael A.", 
            "id": "sg:person.0743735252.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743735252.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8.", 
                "Department of Molecular and Medical Genetics, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Davidson", 
            "givenName": "Alan R.", 
            "id": "sg:person.0776437310.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776437310.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9.", 
                "Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8.", 
                "Department of Molecular and Medical Genetics, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pai", 
            "givenName": "Emil F.", 
            "id": "sg:person.01164261766.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164261766.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Yale University", 
              "id": "https://www.grid.ac/institutes/grid.47100.32", 
              "name": [
                "Department of Molecular Biophysics and Biochemistry and Computer Science, PO Box 208114, Yale University, New Haven , Connecticut 06520, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gerstein", 
            "givenName": "Mark", 
            "id": "sg:person.011677655217.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011677655217.83"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9.", 
                "Banting and Best Department of Medical Research, C.H. Best Institute, University of Toronto, 112 College St., Toronto, Ontario, Canada M5G 1L6."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Edwards", 
            "givenName": "Aled M.", 
            "id": "sg:person.0745553521.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745553521.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Arrowsmith", 
            "givenName": "Cheryl H.", 
            "id": "sg:person.01143744003.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143744003.44"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1002/pro.5560070226", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001969421"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pro.5560070226", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001969421"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.95.26.15189", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002904894"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.97.12.6316", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013236241"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6976.1998.tb00371.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013486571"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(05)80360-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013618994"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.97.12.6311", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014929775"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0134(1997)1+<2::aid-prot2>3.0.co;2-t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015030749"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.95.23.13597", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018446959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/jmbi.1993.1489", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019247667"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/4136", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020959260", 
              "https://doi.org/10.1038/4136"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1006968328186", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025541396", 
              "https://doi.org/10.1023/a:1006968328186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/jmbi.1993.1413", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032431006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pro.5560041020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035211955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/bbrc.1998.9893", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044257048"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(85)15006-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046327081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.050417797", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051482655"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00129a007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055161988"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.288.5466.640", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062569305"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2000-10-01", 
        "datePublishedReg": "2000-10-01", 
        "description": "A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/82823", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1295033", 
            "issn": [
              "1545-9993", 
              "2331-365X"
            ], 
            "name": "Nature Structural & Molecular Biology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "10", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "name": "Structural proteomics of an archaeon", 
        "pagination": "nsb1000_903", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "eb5f890d423bd00141dbdc8abb623b43c36056810d38bd2e2c854a264fc19279"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "11017201"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9421566"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/82823"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1049408892"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/82823", 
          "https://app.dimensions.ai/details/publication/pub.1049408892"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T13:56", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nsb1000_903"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/82823'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/82823'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/82823'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/82823'


     

    This table displays all metadata directly associated to this object as RDF triples.

    303 TRIPLES      21 PREDICATES      51 URIs      25 LITERALS      14 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/82823 schema:about N0117d5aa18604ffe97d203507c153f00
    2 N28df2ab505cd4759938f3966dd3d7aee
    3 N84fcf1c31e8e4885975053fb2c67e5e2
    4 N8f25dfeba8ce42249bafdc9d0d77e84b
    5 N9752312ca5174bc7a0c65af46eca5eac
    6 anzsrc-for:06
    7 anzsrc-for:0601
    8 schema:author Nfff66aefb213445a891cef7c7f75ab02
    9 schema:citation sg:pub.10.1023/a:1006968328186
    10 sg:pub.10.1038/4136
    11 https://doi.org/10.1002/(sici)1097-0134(1997)1+<2::aid-prot2>3.0.co;2-t
    12 https://doi.org/10.1002/pro.5560041020
    13 https://doi.org/10.1002/pro.5560070226
    14 https://doi.org/10.1006/bbrc.1998.9893
    15 https://doi.org/10.1006/jmbi.1993.1413
    16 https://doi.org/10.1006/jmbi.1993.1489
    17 https://doi.org/10.1016/0076-6879(85)15006-8
    18 https://doi.org/10.1016/s0022-2836(05)80360-2
    19 https://doi.org/10.1021/bi00129a007
    20 https://doi.org/10.1073/pnas.050417797
    21 https://doi.org/10.1073/pnas.95.23.13597
    22 https://doi.org/10.1073/pnas.95.26.15189
    23 https://doi.org/10.1073/pnas.97.12.6311
    24 https://doi.org/10.1073/pnas.97.12.6316
    25 https://doi.org/10.1111/j.1574-6976.1998.tb00371.x
    26 https://doi.org/10.1126/science.288.5466.640
    27 schema:datePublished 2000-10-01
    28 schema:datePublishedReg 2000-10-01
    29 schema:description A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics.
    30 schema:genre research_article
    31 schema:inLanguage en
    32 schema:isAccessibleForFree false
    33 schema:isPartOf N35a36133bee3423d94188a4dc6ed6b65
    34 N6d6da8d3fcaa4e59b13d18e4a2e3d72f
    35 sg:journal.1295033
    36 schema:name Structural proteomics of an archaeon
    37 schema:pagination nsb1000_903
    38 schema:productId N342e7e8d689a42518c009f78b1572d01
    39 N5292f84cff41451aa6fc59155ef5aa38
    40 N704f50afedb84dc28cf08b92ea5f18ac
    41 N82027bf415e344aeaae032795ecfae1b
    42 Nbd11e8b4df6a484eabd1db6ce2d0ee44
    43 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049408892
    44 https://doi.org/10.1038/82823
    45 schema:sdDatePublished 2019-04-10T13:56
    46 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    47 schema:sdPublisher Nd141bcdef38f43c58f3bb9f0622dd0e8
    48 schema:url http://www.nature.com/articles/nsb1000_903
    49 sgo:license sg:explorer/license/
    50 sgo:sdDataset articles
    51 rdf:type schema:ScholarlyArticle
    52 N0117d5aa18604ffe97d203507c153f00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    53 schema:name Protein Conformation
    54 rdf:type schema:DefinedTerm
    55 N20c7c009000d4e5198c23520a14c5bc9 rdf:first sg:person.01301672667.11
    56 rdf:rest N98e7a423c0574ba6aee811fd09bbb410
    57 N28df2ab505cd4759938f3966dd3d7aee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    58 schema:name Crystallography, X-Ray
    59 rdf:type schema:DefinedTerm
    60 N342e7e8d689a42518c009f78b1572d01 schema:name pubmed_id
    61 schema:value 11017201
    62 rdf:type schema:PropertyValue
    63 N353cd32911c24717a7aa5582f7665381 rdf:first sg:person.011677655217.83
    64 rdf:rest Nde9e850e9ebb4b1a8d99cafe151e34bb
    65 N35a36133bee3423d94188a4dc6ed6b65 schema:issueNumber 10
    66 rdf:type schema:PublicationIssue
    67 N4292fcc10771438bbb41e1f29b30b2f5 rdf:first sg:person.0576253234.08
    68 rdf:rest Ned1794d14267407db13d755cf534557c
    69 N5292f84cff41451aa6fc59155ef5aa38 schema:name nlm_unique_id
    70 schema:value 9421566
    71 rdf:type schema:PropertyValue
    72 N5d4d29bfc8944f51a60ade066c7e290c rdf:first sg:person.01324255516.18
    73 rdf:rest Nde96afec76ad4e81b5736cf13ed89de3
    74 N609cb62d9dd3406f8c99e43b1766c124 rdf:first sg:person.01143744003.44
    75 rdf:rest rdf:nil
    76 N6d6da8d3fcaa4e59b13d18e4a2e3d72f schema:volumeNumber 7
    77 rdf:type schema:PublicationVolume
    78 N704f50afedb84dc28cf08b92ea5f18ac schema:name doi
    79 schema:value 10.1038/82823
    80 rdf:type schema:PropertyValue
    81 N75c31aee963545c5aa58f07ff179c960 rdf:first sg:person.015766703057.43
    82 rdf:rest Ne645c4e0b87f4931ad1f3e5034693946
    83 N77fe692d8e8647a68a2cad72b5c9ade2 rdf:first sg:person.01164261766.27
    84 rdf:rest N353cd32911c24717a7aa5582f7665381
    85 N7ac7115186a14b2b907022e34ec296b6 rdf:first sg:person.0657214023.64
    86 rdf:rest N5d4d29bfc8944f51a60ade066c7e290c
    87 N7bda58729c2c4ef5b7c45b291a65773a rdf:first sg:person.01261053663.24
    88 rdf:rest N20c7c009000d4e5198c23520a14c5bc9
    89 N82027bf415e344aeaae032795ecfae1b schema:name readcube_id
    90 schema:value eb5f890d423bd00141dbdc8abb623b43c36056810d38bd2e2c854a264fc19279
    91 rdf:type schema:PropertyValue
    92 N84fcf1c31e8e4885975053fb2c67e5e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Methanobacterium
    94 rdf:type schema:DefinedTerm
    95 N8f25dfeba8ce42249bafdc9d0d77e84b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Cloning, Molecular
    97 rdf:type schema:DefinedTerm
    98 N9752312ca5174bc7a0c65af46eca5eac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Proteome
    100 rdf:type schema:DefinedTerm
    101 N98e7a423c0574ba6aee811fd09bbb410 rdf:first sg:person.0776013027.27
    102 rdf:rest N4292fcc10771438bbb41e1f29b30b2f5
    103 Na851ee6d3b4a4273a8fcafdfc1234140 rdf:first sg:person.01140615260.57
    104 rdf:rest Nd7fea4bad13946cb8eedcbda32019977
    105 Nbd11e8b4df6a484eabd1db6ce2d0ee44 schema:name dimensions_id
    106 schema:value pub.1049408892
    107 rdf:type schema:PropertyValue
    108 Nc13fed918ee44b038ba715307f1cabfa rdf:first sg:person.0731526665.51
    109 rdf:rest N75c31aee963545c5aa58f07ff179c960
    110 Nd141bcdef38f43c58f3bb9f0622dd0e8 schema:name Springer Nature - SN SciGraph project
    111 rdf:type schema:Organization
    112 Nd7fea4bad13946cb8eedcbda32019977 rdf:first sg:person.01142325477.65
    113 rdf:rest Nc13fed918ee44b038ba715307f1cabfa
    114 Nde96afec76ad4e81b5736cf13ed89de3 rdf:first sg:person.0743735252.37
    115 rdf:rest Ne2559ce510fe40489a4502a067a7091f
    116 Nde9e850e9ebb4b1a8d99cafe151e34bb rdf:first sg:person.0745553521.55
    117 rdf:rest N609cb62d9dd3406f8c99e43b1766c124
    118 Ne2559ce510fe40489a4502a067a7091f rdf:first sg:person.0776437310.36
    119 rdf:rest N77fe692d8e8647a68a2cad72b5c9ade2
    120 Ne645c4e0b87f4931ad1f3e5034693946 rdf:first sg:person.0577535366.93
    121 rdf:rest N7bda58729c2c4ef5b7c45b291a65773a
    122 Ne665eb169ec34bd58fd966df97375f8e rdf:first sg:person.01152355061.36
    123 rdf:rest N7ac7115186a14b2b907022e34ec296b6
    124 Ned1794d14267407db13d755cf534557c rdf:first sg:person.0666544025.28
    125 rdf:rest Ned69089be67d4e46bbe345103d90b6d5
    126 Ned69089be67d4e46bbe345103d90b6d5 rdf:first sg:person.01044552510.53
    127 rdf:rest Ne665eb169ec34bd58fd966df97375f8e
    128 Nfff66aefb213445a891cef7c7f75ab02 rdf:first sg:person.01347341027.78
    129 rdf:rest Na851ee6d3b4a4273a8fcafdfc1234140
    130 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    131 schema:name Biological Sciences
    132 rdf:type schema:DefinedTerm
    133 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    134 schema:name Biochemistry and Cell Biology
    135 rdf:type schema:DefinedTerm
    136 sg:journal.1295033 schema:issn 1545-9993
    137 2331-365X
    138 schema:name Nature Structural & Molecular Biology
    139 rdf:type schema:Periodical
    140 sg:person.01044552510.53 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    141 schema:familyName Maxwell
    142 schema:givenName Karen L.
    143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044552510.53
    144 rdf:type schema:Person
    145 sg:person.01140615260.57 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    146 schema:familyName Yee
    147 schema:givenName Adelinda
    148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140615260.57
    149 rdf:type schema:Person
    150 sg:person.01142325477.65 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    151 schema:familyName Dharamsi
    152 schema:givenName Akil
    153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142325477.65
    154 rdf:type schema:Person
    155 sg:person.01143744003.44 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    156 schema:familyName Arrowsmith
    157 schema:givenName Cheryl H.
    158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143744003.44
    159 rdf:type schema:Person
    160 sg:person.01152355061.36 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    161 schema:familyName Wu
    162 schema:givenName Ning
    163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152355061.36
    164 rdf:type schema:Person
    165 sg:person.01164261766.27 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    166 schema:familyName Pai
    167 schema:givenName Emil F.
    168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164261766.27
    169 rdf:type schema:Person
    170 sg:person.011677655217.83 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
    171 schema:familyName Gerstein
    172 schema:givenName Mark
    173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011677655217.83
    174 rdf:type schema:Person
    175 sg:person.01261053663.24 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    176 schema:familyName Booth
    177 schema:givenName Valerie
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261053663.24
    179 rdf:type schema:Person
    180 sg:person.01301672667.11 schema:affiliation https://www.grid.ac/institutes/grid.17091.3e
    181 schema:familyName Mackereth
    182 schema:givenName Cameron D.
    183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301672667.11
    184 rdf:type schema:Person
    185 sg:person.01324255516.18 schema:affiliation https://www.grid.ac/institutes/grid.14709.3b
    186 schema:familyName Gehring
    187 schema:givenName Kalle
    188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324255516.18
    189 rdf:type schema:Person
    190 sg:person.01347341027.78 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    191 schema:familyName Christendat
    192 schema:givenName Dinesh
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347341027.78
    194 rdf:type schema:Person
    195 sg:person.015766703057.43 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    196 schema:familyName Savchenko
    197 schema:givenName Alexei
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015766703057.43
    199 rdf:type schema:Person
    200 sg:person.0576253234.08 schema:affiliation https://www.grid.ac/institutes/grid.443931.8
    201 schema:familyName Ekiel
    202 schema:givenName Irena
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576253234.08
    204 rdf:type schema:Person
    205 sg:person.0577535366.93 schema:affiliation https://www.grid.ac/institutes/grid.451303.0
    206 schema:familyName Cort
    207 schema:givenName John R.
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577535366.93
    209 rdf:type schema:Person
    210 sg:person.0657214023.64 schema:affiliation https://www.grid.ac/institutes/grid.17091.3e
    211 schema:familyName McIntosh
    212 schema:givenName Lawrence P.
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657214023.64
    214 rdf:type schema:Person
    215 sg:person.0666544025.28 schema:affiliation https://www.grid.ac/institutes/grid.14709.3b
    216 schema:familyName Kozlov
    217 schema:givenName Guennadi
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666544025.28
    219 rdf:type schema:Person
    220 sg:person.0731526665.51 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
    221 schema:familyName Kluger
    222 schema:givenName Yuval
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731526665.51
    224 rdf:type schema:Person
    225 sg:person.0743735252.37 schema:affiliation https://www.grid.ac/institutes/grid.451303.0
    226 schema:familyName Kennedy
    227 schema:givenName Michael A.
    228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743735252.37
    229 rdf:type schema:Person
    230 sg:person.0745553521.55 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    231 schema:familyName Edwards
    232 schema:givenName Aled M.
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745553521.55
    234 rdf:type schema:Person
    235 sg:person.0776013027.27 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    236 schema:familyName Saridakis
    237 schema:givenName Vivian
    238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776013027.27
    239 rdf:type schema:Person
    240 sg:person.0776437310.36 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    241 schema:familyName Davidson
    242 schema:givenName Alan R.
    243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776437310.36
    244 rdf:type schema:Person
    245 sg:pub.10.1023/a:1006968328186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025541396
    246 https://doi.org/10.1023/a:1006968328186
    247 rdf:type schema:CreativeWork
    248 sg:pub.10.1038/4136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020959260
    249 https://doi.org/10.1038/4136
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1002/(sici)1097-0134(1997)1+<2::aid-prot2>3.0.co;2-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1015030749
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1002/pro.5560041020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035211955
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1002/pro.5560070226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001969421
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1006/bbrc.1998.9893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044257048
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1006/jmbi.1993.1413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032431006
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1006/jmbi.1993.1489 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019247667
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1016/0076-6879(85)15006-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046327081
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1016/s0022-2836(05)80360-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013618994
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1021/bi00129a007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055161988
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1073/pnas.050417797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051482655
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1073/pnas.95.23.13597 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018446959
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1073/pnas.95.26.15189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002904894
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1073/pnas.97.12.6311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014929775
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1073/pnas.97.12.6316 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013236241
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1111/j.1574-6976.1998.tb00371.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013486571
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1126/science.288.5466.640 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062569305
    282 rdf:type schema:CreativeWork
    283 https://www.grid.ac/institutes/grid.14709.3b schema:alternateName McGill University
    284 schema:name Department of Biochemistry and Montreal Joint Centre for Structural Biology, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada , H3G 1Y6.
    285 rdf:type schema:Organization
    286 https://www.grid.ac/institutes/grid.17063.33 schema:alternateName University of Toronto
    287 schema:name Banting and Best Department of Medical Research, C.H. Best Institute, University of Toronto, 112 College St., Toronto, Ontario, Canada M5G 1L6.
    288 Department of Biochemistry, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8.
    289 Department of Molecular and Medical Genetics, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8.
    290 Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9.
    291 rdf:type schema:Organization
    292 https://www.grid.ac/institutes/grid.17091.3e schema:alternateName University of British Columbia
    293 schema:name Departments of Biochemistry and Molecular Biology, Chemistry and the Biotechnology Laboratory, 2146 Health Sciences Mall, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.
    294 rdf:type schema:Organization
    295 https://www.grid.ac/institutes/grid.443931.8 schema:alternateName Biotechnology Research Institute
    296 schema:name Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Ave., Montreal , Quebec, Canada H4P 2R2.
    297 rdf:type schema:Organization
    298 https://www.grid.ac/institutes/grid.451303.0 schema:alternateName Pacific Northwest National Laboratory
    299 schema:name Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratories, EMSL 2569 K8-98, Richland, Washington 99352, USA.
    300 rdf:type schema:Organization
    301 https://www.grid.ac/institutes/grid.47100.32 schema:alternateName Yale University
    302 schema:name Department of Molecular Biophysics and Biochemistry and Computer Science, PO Box 208114, Yale University, New Haven , Connecticut 06520, USA.
    303 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...