Structural proteomics of an archaeon View Full Text


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Article Info

DATE

2000-10-01

AUTHORS

Dinesh Christendat, Adelinda Yee, Akil Dharamsi, Yuval Kluger, Alexei Savchenko, John R. Cort, Valerie Booth, Cameron D. Mackereth, Vivian Saridakis, Irena Ekiel, Guennadi Kozlov, Karen L. Maxwell, Ning Wu, Lawrence P. McIntosh, Kalle Gehring, Michael A. Kennedy, Alan R. Davidson, Emil F. Pai, Mark Gerstein, Aled M. Edwards, Cheryl H. Arrowsmith

ABSTRACT

A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics. More... »

PAGES

nsb1000_903

References to SciGraph publications

  • 1999-03. Identification of the archaeal NMN adenylyltransferase gene in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 1998-12. 100,000 protein structures for the biologist in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/82823

    DOI

    http://dx.doi.org/10.1038/82823

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049408892

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11017201


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/82823'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/82823'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/82823'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/82823'


     

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