Simultaneous stochastic sensing of divalent metal ions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-09

AUTHORS

O Braha, L Q Gu, L Zhou, X Lu, S Cheley, H Bayley

ABSTRACT

Stochastic sensing is an emerging analytical technique that relies upon single-molecule detection. Transmembrane pores, into which binding sites for analytes have been placed by genetic engineering, have been developed as stochastic sensing elements. Reversible occupation of an engineered binding site modulates the ionic current passing through a pore in a transmembrane potential and thereby provides both the concentration of an analyte and, through a characteristic signature, its identity. Here, we show that the concentrations of two or more divalent metal ions in solution can be determined simultaneously with a single sensor element. Further, the sensor element can be permanently calibrated without a detailed understanding of the kinetics of interaction of the metal ions with the engineered pore. More... »

PAGES

1005-1007

Journal

TITLE

Nature Biotechnology

ISSUE

9

VOLUME

18

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/79275

DOI

http://dx.doi.org/10.1038/79275

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027719967

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10973225


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