Single nucleotide polymorphic discrimination by an electronic dot blot assay on semiconductor microchips View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-04

AUTHORS

Patrick N. Gilles, David J. Wu, Charles B. Foster, Patrick J. Dillon, Stephen J. Chanock

ABSTRACT

We have developed a rapid assay for single nucleotide polymorphism (SNP) detection that utilizes electronic circuitry on silicon microchips. The method was validated by the accurate discrimination of blinded DNA samples for the complex quadra-allelic SNP of mannose binding protein. The microchip directed the transport, concentration, and attachment of amplified patient DNA to selected electrodes (test sites) creating an array of DNA samples. Through control of the electric field, the microchip enabled accurate genetic identification of these samples using fluorescently labeled DNA reporter probes. The accuracy of this approach was established by internal controls of dual labeled reporters and by using mismatched sequences in addition to the wild-type and variant reporter sequences to validate the SNP-genotype. The ability to customize this assay for multiple genes has advantages over other existing approaches. More... »

PAGES

nbt0499_365

Journal

TITLE

Nature Biotechnology

ISSUE

4

VOLUME

17

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/7921

    DOI

    http://dx.doi.org/10.1038/7921

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1019407436

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10207885


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