Direct haplotyping of kilobase-size DNA using carbon nanotube probes View Full Text


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Article Info

DATE

2000-07

AUTHORS

Adam T. Woolley, Chantal Guillemette, Chin Li Cheung, David E. Housman, Charles M. Lieber

ABSTRACT

We have implemented a method for multiplexed detection of polymorphic sites and direct determination of haplotypes in 10-kilobase-size DNA fragments using single-walled carbon nanotube (SWNT) atomic force microscopy (AFM) probes. Labeled oligonucleotides are hybridized specifically to complementary target sequences in template DNA, and the positions of the tagged sequences are detected by direct SWNT tip imaging. We demonstrated this concept by detecting streptavidin and IRD800 labels at two different sequences in M13mp18. Our approach also permits haplotype determination from simple visual inspection of AFM images of individual DNA molecules, which we have done on UGT1A7, a gene under study as a cancer risk factor. The haplotypes of individuals heterozygous at two critical loci, which together influence cancer risk, can be easily and directly distinguished from AFM images. The application of this technique to haplotyping in population-based genetic disease studies and other genomic screening problems is discussed. More... »

PAGES

760

Journal

TITLE

Nature Biotechnology

ISSUE

7

VOLUME

18

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/77760

    DOI

    http://dx.doi.org/10.1038/77760

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035209552

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10888845


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