Targeted screening for induced mutations View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-04

AUTHORS

C M McCallum, L Comai, E A Greene, S Henikoff

ABSTRACT

With the accumulation of large-scale sequence data, emphasis in genomics has shifted from determining gene structure to testing gene function, and this relies on reverse genetic methodology. Here we explore the feasibility of screening for chemically induced mutations in target sequences in Arabidopsis thaliana. Our TILLING (Targeting Induced Local Lesions IN Genomes) method combines the efficiency of ethyl methanesulfonate (EMS)-induced mutagenesis with the ability of denaturing high-performance liquid chromatography (DHPLC) to detect base pair changes by heteroduplex analysis. Importantly, this method generates a wide range of mutant alleles, is fast and automatable, and is applicable to any organism that can be chemically mutagenized. More... »

PAGES

455-457

References to SciGraph publications

Journal

TITLE

Nature Biotechnology

ISSUE

4

VOLUME

18

Related Patents

  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Oil Content
  • Generation Of Plants With Altered Oil Content
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Resistance To Auxinic Herbicides
  • Disease Resistant Plants
  • Generation Of Plants With Altered Oil, Protein Or Fiber Content
  • Transgenic Plants Having Altered Biomass Composition
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Disruption Of Ahp6 Gene Leads To Plants With Improved Seed Yield
  • Disease Resistant Grape Plants
  • Transgenic Plants Having Increased Tolerance To Aluminum
  • Resistance To Auxinic Herbicides
  • Brassica Rod1 Gene Sequences And Uses Thereof
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Organism With Altered Carotenoid Content And Method Of Producing Same
  • Maize Variety And Method Of Production
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Oil Content
  • Disruption Of Ckx3 And At Least One Other Ckx Gene In A Plant Or Plant Cell Leads To Improved Traits
  • Tomatoes Having Reduced Polygalacturonase Activity Caused By Non-Transgenic Mutations In The Polygalacturonase Gene
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Oil Content
  • Generation Of Plants With Altered Oil Content
  • Wheat Having Reduced Waxy Protein Due To Non-Transgenic Alterations Of A Waxy Gene
  • Disease Resistant Tomato Plants
  • Identification And The Use Of Krp Mutants In Plants
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Oil Content
  • Generation Of Plants With Altered Oil Content
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Generation Of Plants With Altered Oil Content
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Generation Of Plants With Altered Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Resistance To Auxinic Herbicides
  • Disease Resistant Plants
  • Brassica Plant Comprising A Mutant Indehiscent Allele
  • Soybean Rod1 Gene Sequences And Uses Thereof
  • Plants Modified For Reduced Cadmium Transport, Derivative Products, And Related Methods
  • Generation Of Plants With Altered Oil Content
  • Resistance To Auxinic Herbicides
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Oil Content
  • Disruption Of Acc Synthase Genes To Delay Senescence In Plants
  • Transgenic Plants Having Increased Biomass
  • Plants Modified For Reduced Cadmium Transport, Derivative Products, And Related Methods
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Resistance To Auxinic Herbicides
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Oil Content
  • Gossypium Hirsutum Plants With Increased Fiber Strength Comprising A Fiber Strength Allele Spanning The Gluc1.1a Gene From Gossypium Barbadense
  • Generation Of Plants With Altered Oil Content
  • Nicotiana Benthamiana Plants Deficient In Xylosyltransferase Activity
  • Generation Of Plants With Altered Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Tomatoes Having Reduced Polygalacturonase Activity Caused By Non-Transgenic Mutations In The Polygalacturonase Gene
  • Means And Methods For The Reduction Of Photorespiration In Crops
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Soybeans With Reduced Isoflavones
  • Generation Of Plants With Altered Oil, Protein, Or Fiber Content
  • Disease Resistant Plants
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Disease Resistant Plants
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/74542

    DOI

    http://dx.doi.org/10.1038/74542

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1000399665

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10748531


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Alleles", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Substitution", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Arabidopsis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Pair Mismatch", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Pairing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromatography, High Pressure Liquid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Codon, Terminator", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Conserved Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA (Cytosine-5-)-Methyltransferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Modification Methylases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Primers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ethyl Methanesulfonate", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Introns", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutagenesis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Point Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Fred Hutchinson Cancer Research Center", 
              "id": "https://www.grid.ac/institutes/grid.270240.3", 
              "name": [
                "Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "McCallum", 
            "givenName": "C M", 
            "id": "sg:person.0605606371.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605606371.02"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Comai", 
            "givenName": "L", 
            "id": "sg:person.01042217474.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042217474.55"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Greene", 
            "givenName": "E A", 
            "id": "sg:person.0763374436.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763374436.17"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Henikoff", 
            "givenName": "S", 
            "id": "sg:person.01110332674.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110332674.32"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1093/nar/26.7.1628", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007625044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1224::aid-elps1224>3.0.co;2-o", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021393785"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1224::aid-elps1224>3.0.co;2-o", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021393785"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.1.1.37", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023274746"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.9.9.859", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025812109"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0027-5107(82)90129-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028458523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/26.20.4597", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030448908"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/17.7.2421", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033603099"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1098-1004(1999)13:4<318::aid-humu9>3.0.co;2-f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036583964"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/25.22.4692", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043831325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004399900092", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049621079", 
              "https://doi.org/10.1007/s004399900092"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004399900092", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049621079", 
              "https://doi.org/10.1007/s004399900092"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac990099w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055076581"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac990099w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055076581"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/gte.1997.1.201", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059267008"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074500492", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1995.tb00069.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082456783"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.7.10.996", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083159140"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083259836", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.8.7.698", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083298508"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2000-04", 
        "datePublishedReg": "2000-04-01", 
        "description": "With the accumulation of large-scale sequence data, emphasis in genomics has shifted from determining gene structure to testing gene function, and this relies on reverse genetic methodology. Here we explore the feasibility of screening for chemically induced mutations in target sequences in Arabidopsis thaliana. Our TILLING (Targeting Induced Local Lesions IN Genomes) method combines the efficiency of ethyl methanesulfonate (EMS)-induced mutagenesis with the ability of denaturing high-performance liquid chromatography (DHPLC) to detect base pair changes by heteroduplex analysis. Importantly, this method generates a wide range of mutant alleles, is fast and automatable, and is applicable to any organism that can be chemically mutagenized.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/74542", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2509733", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "18"
          }
        ], 
        "name": "Targeted screening for induced mutations", 
        "pagination": "455-457", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "01e739308365b1d2518fa49b5c27cfb7701af53c4638def54575f1cfb6a37ed6"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10748531"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/74542"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1000399665"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/74542", 
          "https://app.dimensions.ai/details/publication/pub.1000399665"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:21", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87079_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/nbt/journal/v18/n4/full/nbt0400_455.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/74542'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/74542'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/74542'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/74542'


     

    This table displays all metadata directly associated to this object as RDF triples.

    215 TRIPLES      21 PREDICATES      65 URIs      40 LITERALS      28 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/74542 schema:about N069be1f85083427f84f9368620609a21
    2 N09c8823c91dc418aa789f8e44e6a2e73
    3 N0e15935aae8d45c29c4b8c3b31a8e954
    4 N14cbab4c5b564c0cbb11b4598c1ee3ff
    5 N1f6603fd59e74e28a085aaa4c5fcb1b7
    6 N2619675582ce42d08693f505b0e9175b
    7 N3575bc944e6d409ba10ebf53bc9b2c85
    8 N463f5fedd64446f5a0e73f17474efbd5
    9 N548112a131a54db1a66fa36a0a38f9b0
    10 N58ae94c0f76d4e049ddd836c00f7bd21
    11 N67c0c7eb2d8f4bc7ac46b5fb34643284
    12 N8be7be29f87d40a28145a6d452293443
    13 N91206708759f4a1d8b933d2a133a0add
    14 Naec0ffea92574e8c890405a44685e393
    15 Nbbf3914ccdfc4614b111e9b9d2fc60f7
    16 Nc1e4b1b6fe0942e08e25514536f18418
    17 Nc4bcd964b6104b438fca60749efedeb5
    18 Nd9e8c62a477a4519b78b2481435207b1
    19 Nfff9a906b16147769aabb08fc78edf22
    20 anzsrc-for:06
    21 anzsrc-for:0604
    22 schema:author Naf15cc0b03a84dc29abad2a5981acf90
    23 schema:citation sg:pub.10.1007/s004399900092
    24 https://app.dimensions.ai/details/publication/pub.1074500492
    25 https://app.dimensions.ai/details/publication/pub.1083259836
    26 https://doi.org/10.1002/(sici)1098-1004(1999)13:4<318::aid-humu9>3.0.co;2-f
    27 https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1224::aid-elps1224>3.0.co;2-o
    28 https://doi.org/10.1002/j.1460-2075.1995.tb00069.x
    29 https://doi.org/10.1016/0027-5107(82)90129-4
    30 https://doi.org/10.1021/ac990099w
    31 https://doi.org/10.1089/gte.1997.1.201
    32 https://doi.org/10.1093/nar/17.7.2421
    33 https://doi.org/10.1093/nar/25.22.4692
    34 https://doi.org/10.1093/nar/26.20.4597
    35 https://doi.org/10.1093/nar/26.7.1628
    36 https://doi.org/10.1101/gr.7.10.996
    37 https://doi.org/10.1101/gr.8.7.698
    38 https://doi.org/10.1101/gr.9.9.859
    39 https://doi.org/10.1105/tpc.1.1.37
    40 schema:datePublished 2000-04
    41 schema:datePublishedReg 2000-04-01
    42 schema:description With the accumulation of large-scale sequence data, emphasis in genomics has shifted from determining gene structure to testing gene function, and this relies on reverse genetic methodology. Here we explore the feasibility of screening for chemically induced mutations in target sequences in Arabidopsis thaliana. Our TILLING (Targeting Induced Local Lesions IN Genomes) method combines the efficiency of ethyl methanesulfonate (EMS)-induced mutagenesis with the ability of denaturing high-performance liquid chromatography (DHPLC) to detect base pair changes by heteroduplex analysis. Importantly, this method generates a wide range of mutant alleles, is fast and automatable, and is applicable to any organism that can be chemically mutagenized.
    43 schema:genre research_article
    44 schema:inLanguage en
    45 schema:isAccessibleForFree false
    46 schema:isPartOf N2a03aa530cd5488e9018c650b1eb3a04
    47 N7950c8f2493f4a9eaad31df19a0841f8
    48 sg:journal.1115214
    49 schema:name Targeted screening for induced mutations
    50 schema:pagination 455-457
    51 schema:productId N14be3641fa3e40fcbb90e7f03a6e99c5
    52 N363b4effa7154ea9afc439585d5e74c9
    53 N4518beb2e59940f68b0eda575194405c
    54 N991b03ab92644ad8934cf146734762bc
    55 Nb4ef29f268ad40e4b90ebf96140fa114
    56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000399665
    57 https://doi.org/10.1038/74542
    58 schema:sdDatePublished 2019-04-11T12:21
    59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    60 schema:sdPublisher N2ba7c35e871543c0ad535041831e22d7
    61 schema:url http://www.nature.com/nbt/journal/v18/n4/full/nbt0400_455.html
    62 sgo:license sg:explorer/license/
    63 sgo:sdDataset articles
    64 rdf:type schema:ScholarlyArticle
    65 N069be1f85083427f84f9368620609a21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    66 schema:name Codon, Terminator
    67 rdf:type schema:DefinedTerm
    68 N09c8823c91dc418aa789f8e44e6a2e73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    69 schema:name Cloning, Molecular
    70 rdf:type schema:DefinedTerm
    71 N0e15935aae8d45c29c4b8c3b31a8e954 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    72 schema:name Point Mutation
    73 rdf:type schema:DefinedTerm
    74 N14be3641fa3e40fcbb90e7f03a6e99c5 schema:name dimensions_id
    75 schema:value pub.1000399665
    76 rdf:type schema:PropertyValue
    77 N14cbab4c5b564c0cbb11b4598c1ee3ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Mutagenesis
    79 rdf:type schema:DefinedTerm
    80 N1f6603fd59e74e28a085aaa4c5fcb1b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Amino Acid Substitution
    82 rdf:type schema:DefinedTerm
    83 N2619675582ce42d08693f505b0e9175b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Introns
    85 rdf:type schema:DefinedTerm
    86 N2a03aa530cd5488e9018c650b1eb3a04 schema:volumeNumber 18
    87 rdf:type schema:PublicationVolume
    88 N2ba7c35e871543c0ad535041831e22d7 schema:name Springer Nature - SN SciGraph project
    89 rdf:type schema:Organization
    90 N34105e2dd5a047a99715a71fde67385b rdf:first sg:person.01042217474.55
    91 rdf:rest Nae888050df7943fba58ef2dc93fb52ab
    92 N3575bc944e6d409ba10ebf53bc9b2c85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Alleles
    94 rdf:type schema:DefinedTerm
    95 N363b4effa7154ea9afc439585d5e74c9 schema:name nlm_unique_id
    96 schema:value 9604648
    97 rdf:type schema:PropertyValue
    98 N4518beb2e59940f68b0eda575194405c schema:name doi
    99 schema:value 10.1038/74542
    100 rdf:type schema:PropertyValue
    101 N463f5fedd64446f5a0e73f17474efbd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name DNA (Cytosine-5-)-Methyltransferases
    103 rdf:type schema:DefinedTerm
    104 N548112a131a54db1a66fa36a0a38f9b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name DNA Modification Methylases
    106 rdf:type schema:DefinedTerm
    107 N58ae94c0f76d4e049ddd836c00f7bd21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Ethyl Methanesulfonate
    109 rdf:type schema:DefinedTerm
    110 N67c0c7eb2d8f4bc7ac46b5fb34643284 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Polymerase Chain Reaction
    112 rdf:type schema:DefinedTerm
    113 N756ab293e0174698be086693c362845d rdf:first sg:person.01110332674.32
    114 rdf:rest rdf:nil
    115 N7950c8f2493f4a9eaad31df19a0841f8 schema:issueNumber 4
    116 rdf:type schema:PublicationIssue
    117 N8be7be29f87d40a28145a6d452293443 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Arabidopsis
    119 rdf:type schema:DefinedTerm
    120 N91206708759f4a1d8b933d2a133a0add schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name DNA Primers
    122 rdf:type schema:DefinedTerm
    123 N991b03ab92644ad8934cf146734762bc schema:name readcube_id
    124 schema:value 01e739308365b1d2518fa49b5c27cfb7701af53c4638def54575f1cfb6a37ed6
    125 rdf:type schema:PropertyValue
    126 Nae888050df7943fba58ef2dc93fb52ab rdf:first sg:person.0763374436.17
    127 rdf:rest N756ab293e0174698be086693c362845d
    128 Naec0ffea92574e8c890405a44685e393 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Conserved Sequence
    130 rdf:type schema:DefinedTerm
    131 Naf15cc0b03a84dc29abad2a5981acf90 rdf:first sg:person.0605606371.02
    132 rdf:rest N34105e2dd5a047a99715a71fde67385b
    133 Nb4ef29f268ad40e4b90ebf96140fa114 schema:name pubmed_id
    134 schema:value 10748531
    135 rdf:type schema:PropertyValue
    136 Nbbf3914ccdfc4614b111e9b9d2fc60f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Base Pair Mismatch
    138 rdf:type schema:DefinedTerm
    139 Nc1e4b1b6fe0942e08e25514536f18418 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Chromatography, High Pressure Liquid
    141 rdf:type schema:DefinedTerm
    142 Nc4bcd964b6104b438fca60749efedeb5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Base Sequence
    144 rdf:type schema:DefinedTerm
    145 Nd9e8c62a477a4519b78b2481435207b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Base Pairing
    147 rdf:type schema:DefinedTerm
    148 Nfff9a906b16147769aabb08fc78edf22 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Genetic Engineering
    150 rdf:type schema:DefinedTerm
    151 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    152 schema:name Biological Sciences
    153 rdf:type schema:DefinedTerm
    154 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Genetics
    156 rdf:type schema:DefinedTerm
    157 sg:grant.2509733 http://pending.schema.org/fundedItem sg:pub.10.1038/74542
    158 rdf:type schema:MonetaryGrant
    159 sg:journal.1115214 schema:issn 1087-0156
    160 1546-1696
    161 schema:name Nature Biotechnology
    162 rdf:type schema:Periodical
    163 sg:person.01042217474.55 schema:familyName Comai
    164 schema:givenName L
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042217474.55
    166 rdf:type schema:Person
    167 sg:person.01110332674.32 schema:familyName Henikoff
    168 schema:givenName S
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110332674.32
    170 rdf:type schema:Person
    171 sg:person.0605606371.02 schema:affiliation https://www.grid.ac/institutes/grid.270240.3
    172 schema:familyName McCallum
    173 schema:givenName C M
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605606371.02
    175 rdf:type schema:Person
    176 sg:person.0763374436.17 schema:familyName Greene
    177 schema:givenName E A
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763374436.17
    179 rdf:type schema:Person
    180 sg:pub.10.1007/s004399900092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049621079
    181 https://doi.org/10.1007/s004399900092
    182 rdf:type schema:CreativeWork
    183 https://app.dimensions.ai/details/publication/pub.1074500492 schema:CreativeWork
    184 https://app.dimensions.ai/details/publication/pub.1083259836 schema:CreativeWork
    185 https://doi.org/10.1002/(sici)1098-1004(1999)13:4<318::aid-humu9>3.0.co;2-f schema:sameAs https://app.dimensions.ai/details/publication/pub.1036583964
    186 rdf:type schema:CreativeWork
    187 https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1224::aid-elps1224>3.0.co;2-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1021393785
    188 rdf:type schema:CreativeWork
    189 https://doi.org/10.1002/j.1460-2075.1995.tb00069.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082456783
    190 rdf:type schema:CreativeWork
    191 https://doi.org/10.1016/0027-5107(82)90129-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028458523
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1021/ac990099w schema:sameAs https://app.dimensions.ai/details/publication/pub.1055076581
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1089/gte.1997.1.201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059267008
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1093/nar/17.7.2421 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033603099
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1093/nar/25.22.4692 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043831325
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1093/nar/26.20.4597 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030448908
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1093/nar/26.7.1628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007625044
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1101/gr.7.10.996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083159140
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1101/gr.8.7.698 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083298508
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1101/gr.9.9.859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025812109
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1105/tpc.1.1.37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023274746
    212 rdf:type schema:CreativeWork
    213 https://www.grid.ac/institutes/grid.270240.3 schema:alternateName Fred Hutchinson Cancer Research Center
    214 schema:name Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.
    215 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...