Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-04

AUTHORS

Elena Zubko, Charles Scutt, Peter Meyer

ABSTRACT

Recombinant genes conferring resistance to antibiotics or herbicides are widely used as selectable markers in plant transformation. Once transgenic material has been selected, the marker gene is dispensable. We report a novel strategy to remove undesirable parts of a transgene after integration into the tobacco genome. This approach is based on the transfer of a vector containing a NPTII gene flanked by two 352 bp attachment P (attP) regions of bacteriophage lambda, and the identification of somatic tissue with deletion events following intrachromosomal recombination between the attP regions. This system was used to delete a 5.9 kb region from a recombinant vector that had been inserted into two different genomic regions. As the attP system does not require the expression of helper proteins to induce deletion events, or a genetic segregation step to remove recombinase genes, it should provide a useful tool to remove undesirable transgene regions, especially in vegetatively propagated species. More... »

PAGES

nbt0400_442

References to SciGraph publications

  • 1994. Specialized vectors for gene tagging and expression studies in PLANT MOLECULAR BIOLOGY MANUAL
  • 1995-05. Somatic intrachromosomal homologous recombination events in populations of plant siblings in PLANT MOLECULAR BIOLOGY
  • 1994-03. Transformation Systems for Generating Marker–Free Transgenic Plants in NATURE BIOTECHNOLOGY
  • 1994. Substrate Specificity of Plant Recombinases Determined in Extrachromosomal Recombination Systems in HOMOLOGOUS RECOMBINATION AND GENE SILENCING IN PLANTS
  • 1988-01. A negative selection scheme for tobacco protoplast-derived cells expressing the T-DNA gene 2 in PLANT CELL REPORTS
  • 1991-05. Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequences in PLANT MOLECULAR BIOLOGY
  • 1987-03. The chalcone synthase multigene family of Petunia hybrida (V30): sequence homology, chromosomal localization and evolutionary aspects in PLANT MOLECULAR BIOLOGY
  • 1991-09. Two T-DNA's co-transformed intoBrassica napus by a doubleAgrobacterium tumefaciens infection are mainly integrated at the same locus in THEORETICAL AND APPLIED GENETICS
  • 1993-02. Somatic homologous recombination in planta: The recombination frequency is dependent on the allelic state of recombining sequences and may be influenced by genomic position effects in MOLECULAR GENETICS AND GENOMICS
  • 1995-09. The transformation booster sequence from Petunia hybrida is a retrotransposon derivative that binds to the nuclear scaffold in MOLECULAR GENETICS AND GENOMICS
  • 1986-09. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector in MOLECULAR GENETICS AND GENOMICS
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/74515

    DOI

    http://dx.doi.org/10.1038/74515

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011877455

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10748528


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