Identification of in vivo DNA targets of chromatin proteins using tethered Dam methyltransferase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-04

AUTHORS

B van Steensel, S Henikoff

ABSTRACT

We have developed a novel technique, named DamID, for the identification of DNA loci that interact in vivo with specific nuclear proteins in eukaryotes. By tethering Escherichia coli DNA adenine methyltransferase (Dam) to a chromatin protein, Dam can be targeted in vivo to native binding sites of this protein, resulting in local DNA methylation. Sites of methylation can subsequently be mapped using methylation-specific restriction enzymes or antibodies. We demonstrate the successful application of DamID both in Drosophila cell cultures and in whole flies. When Dam is tethered to the DNA-binding domain of GAL4, targeted methylation is limited to a region of a few kilobases surrounding a GAL4 binding sequence. Using DamID, we identified a number of expected and unexpected target loci for Drosophila heterochromatin protein 1. DamID has potential for genome-wide mapping of in vivo targets of chromatin proteins in various eukaryotes. More... »

PAGES

424-428

Journal

TITLE

Nature Biotechnology

ISSUE

4

VOLUME

18

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/74487

    DOI

    http://dx.doi.org/10.1038/74487

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1036546793

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10748524


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