Screening unlabeled DNA targets with randomly ordered fiber-optic gene arrays View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-01

AUTHORS

Frank J. Steemers, Jane A. Ferguson, David R. Walt

ABSTRACT

We have developed a randomly ordered fiber-optic gene array for rapid, parallel detection of unlabeled DNA targets with surface immobilized molecular beacons (MB) that undergo a conformational change accompanied by a fluorescence change in the presence of a complementary DNA target. Microarrays are prepared by randomly distributing MB-functionalized 3-μm diameter microspheres in an array of wells etched in a 500-μm diameter optical imaging fiber. Using several MBs, each designed to recognize a different target, we demonstrate the selective detection of genomic cystic fibrosis related targets. Positional registration and fluorescence response monitoring of the microspheres was performed using an optical encoding scheme and an imaging fluorescence microscope system. More... »

PAGES

91-94

Journal

TITLE

Nature Biotechnology

ISSUE

1

VOLUME

18

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/72006

    DOI

    http://dx.doi.org/10.1038/72006

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024163161

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10625399


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