Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-03

AUTHORS

Victor Lyamichev, Andrea L. Mast, Jeff G. Hall, James R. Prudent, Michael W. Kaiser, Tsetska Takova, Robert W. Kwiatkowski, Tamara J. Sander, Monika de Arruda, David A. Arco, Bruce P. Neri, Mary Ann D. Brow

ABSTRACT

Flap endonucleases (FENs) isolated from archaea are shown to recognize and cleave a structure formed when two overlapping oligonucleotides hybridize to a target DNA strand. The downstream oligonucleotide probe is cleaved, and the precise site of cleavage is dependent on the amount of overlap with the upstream oligonucleotide. We have demonstrated that use of thermostable archaeal FENs allows the reaction to be performed at temperatures that promote probe turnover without the need for temperature cycling. The resulting amplification of the cleavage signal enables the detection of specific DNA targets at sub-attomole levels within complex mixtures. Moreover, we provide evidence that this cleavage is sufficiently specific to enable discrimination of single-base differences and can differentiate homozygotes from heterozygotes in single-copy genes in genomic DNA. More... »

PAGES

nbt0399_292

Journal

TITLE

Nature Biotechnology

ISSUE

3

VOLUME

17

Author Affiliations

Related Patents

  • Nucleic Acid Detection Method Involving The Direct Generation Of A Measurable Signal
  • Nucleic Acid Amplification Method
  • Systems And Methods For Predicting Oligonucleotide Melting Temperature (Tms)
  • Massive Parallel Method For Decoding Dna And Rna
  • Invasive Cleavage Of Nucleic Acids
  • Nucleic Acid Enrichment
  • Fen-1 Endonucleases, Mixtures And Cleavage Methods
  • Removal Of Pcr Inhibitors
  • Detection Of Target Nucleic Acid Sequence By Pto Cleavage And Extension-Dependent Cleavage
  • Compositions And Methods For Preparing Oligonucleotide Solutions
  • Decoding Of Array Sensors With Microspheres
  • Modified Oligonucleotides For Mismatch Discrimination
  • Compositions And Methods For Repetitive Use Of Genomic Dna
  • Compositions And Methods For Performing Methylation Detection Assays
  • Method For Testing Nucleic Acid Sequence
  • Target Analyte Sensors Utilizing Microspheres
  • Combinatorial Decoding Of Random Nucleic Acid Arrays
  • Encoding And Decoding Of Array Sensors Utilizing Nanocrystals
  • Self-Encoding Sensor With Microspheres
  • Compositions And Methods For The Detection Of A Nucleic Acid Using A Cleavage Reaction
  • Methods For Detection Of A Target Nucleic Acid By Capture Using Multi-Subunit Probes
  • Removal Of Pcr Inhibitors
  • Microrna (Mirna) And Downstream Targets For Diagnostic And Therapeutic Purposes
  • Combinatorial Decoding Of Random Nucleic Acid Arrays
  • Detection Of Nucleotide Variation On Target Nucleic Acid Sequence By Pto Cleavage And Extension Assay
  • Pyrosequencing Methods And Related Compositions
  • Detection Of Target Nucleic Acid Sequences By Pto Cleavage And Extension Assay
  • Snap-Back Primers And Detectable Hairpin Structures
  • System And Apparatus For Sequential Processing Of Analytes
  • Detection Of Hpv
  • Detection Of Nucleotide Variation On Target Nucleic Acid Sequence By Pto Cleavage And Extension Assay
  • Detection Of Target Nucleic Acid Sequence By Pto Cleavage And Extension-Dependent Signaling Oligonucleotide Cleavage
  • Self-Encoding Sensor With Microspheres
  • Detection Of Target Nucleic Acid Sequence By Pto Cleavage And Extension-Dependent Signaling Oligonucleotide Hybridization Assay
  • Isolation Of Nucleic Acids
  • Multiplexed Immunohistochemical Assays Using Molecular Tags
  • Compositions For Detection Of A Target Nucleic Acid Sequence
  • Massive Parallel Method For Decoding Dna And Rna
  • Multiplexed Methylation Detection Methods
  • Specialized Oligonucleotides And Their Use In Nucleic Acid Amplification And Detection
  • Methods For Detection Of A Target Nucleic Acid By Forming A Cleavage Structure With A Cleavage Resistant Probe
  • Reactions On A Solid Surface
  • Detection Of Hpv
  • Massive Parallel Method For Decoding Dna And Rna
  • Self-Encoding Sensor With Microspheres
  • Detection Of Nucleic Acid Differences Using Combined Endonuclease Cleavage And Ligation Reactions
  • Massive Parallel Method For Decoding Dna And Rna
  • Detection Of Nucleic Acid Differences Using Combined Endonuclease Cleavage And Ligation Reactions
  • Methods Of Detection Of A Target Nucleic Acid Sequence
  • Use Of Microfluidic Systems In The Detection Of Target Analytes Using Microsphere Arrays
  • Detection Of Target Nucleic Acid Sequences By Pto Cleavage And Extension Assay
  • Methods And Compositions For Detecting Target Sequences
  • Combinatorial Decoding Of Random Nucleic Acid Arrays
  • Method Of Making And Decoding Of Array Sensors With Microspheres
  • Methods For Detection Of A Target Nucleic Acid Sequence
  • Methods For Detection Of A Target Nucleic Acid Sequence
  • Methods For Detection Of A Target Nucleic Acid Using A Probe Comprising Secondary Structure
  • Method For Detecting Nucleic Acid Mutation By Detecting Chemiluminiscence Generated With By-Product Of Complementary Strand Extension Reaction
  • Isolation Of Nucleic Acids
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • Combinatorial Decoding Of Random Nucleic Acid Arrays
  • Synthesis Of Cleavable Fluorescent Nucleotides As Reversible Terminators For Dna Sequencing By Synthesis
  • Tm Leveling Compositions
  • Nucleic Acid Detection Methods Using Universal Priming
  • Methods For Detection Of A Target Nucleic Acid By Capture
  • Parallel Genotyping Of Multiple Patient Samples
  • Compositions And Methods For The Detection Of A Nucleic Acid Using A Cleavage Reaction
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • Microrna (Mirna) And Downstream Targets For Diagnostic And Therapeutic Purposes
  • Massive Parallel Method For Decoding Dna And Rna
  • Use Of Microfluidic Systems In The Detection Of Target Analytes Using Microsphere Arrays
  • Detection Of Target Nucleic Acid Sequences By Pto Cleavage And Extension Assay
  • Amino Acid Transporters
  • Multiplex Genotyping Using Solid Phase Capturable Dideoxynucleotides And Mass Spectrometry
  • Synthesis Of Cleavable Fluorescent Nucleotides As Reversible Terminators For Dna Sequencing By Synthesis
  • Detection Of Nucleotide Variation On Target Nucleic Acid Sequence
  • Methods Of Detecting Targets On An Array
  • Snap-Back Primers And Detectable Hairpin Structures
  • Isolation Of Nucleic Acids
  • Detection Of Target Nucleic Acid Sequence By Pto Cleavage And Extension-Dependent Signaling Oligonucleotide Cleavage
  • Key Probe Compositions And Methods For Polynucleotide Detection
  • Four-Color Dna Sequencing By Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators
  • Massive Parallel Method For Decoding Dna And Rna
  • Isolation Of Nucleic Acids
  • Microrna (Mirna) And Downstream Targets For Diagnostic And Therapeutic Purposes
  • Method For Diagnosing Or Predicting Susceptibility To Optic Neuropathy
  • Multiplex Nucleic Acid Reactions
  • Methods For Detection Of A Target Nucleic Acid By Forming A Cleavage Structure With A Cleavage Resistant Probe
  • Method For Generating A Circularised Nucleic Acid
  • Parallel Genotyping Of Multiple Patient Samples
  • Process For Obtaining Recombined Nucleotide Sequences In Vitro, Libraries Of Sequences And Sequences Thus Obtained
  • Combinatorial Decoding Of Random Nucleic Acid Arrays
  • Snap-Back Primers And Detectable Hairpin Structures
  • Modified Oligonucleotides For Mismatch Discrimination
  • Multiplex Decoding Of Array Sensors With Microspheres
  • Methods For Detection Of A Target Nucleic Acid By Capture Using Multi-Subunit Probes
  • Detection Of Nucleic Acid Amplification Reactions Using Bead Arrays
  • Nucleic Acid Detection Compositions
  • Reactions On A Solid Surface
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • Isolation Of Nucleic Acids
  • Massive Parallel Method For Decoding Dna And Rna
  • Massive Parallel Method For Decoding Dna And Rna
  • Encoding And Decoding Of Array Sensors Utilizing Nanocrystals
  • Multiplexed Detection Of Analytes
  • Self-Encoding Sensor With Microspheres
  • Methods For Detecting Target Analytes And Enzymatic Reactions
  • Detection Of Hpv
  • Digital Sequence Analysis Of Dna Methylation
  • Methods For The Administration Of Iloperidone
  • Methods For The Administration Of Iloperidone
  • Detection Of Target Nucleic Acid Sequences By Pto Cleavage And Extension Assay
  • Detection Of Target Nucleic Acid Sequences By Pto Cleavage And Extension Assay
  • Isolation Of Nucleic Acids
  • Method For Obtaining In Vitro Recombined Polynucleotide Sequences, Sequence Banks And Resulting Sequences
  • Detection Of Target Nucleic Acid Sequence By Pto Cleavage And Extension-Dependent Signaling Oligonucleotide Cleavage
  • Methods For Detecting Target Analytes And Enzymatic Reactions
  • Multiplex Detection Assay For Influenza And Rsv Viruses
  • Compositions And Methods For Preparing Oligonucleotide Solutions
  • Detection Of Target Nucleic Acid Sequences By Po Cleavage And Hybridization
  • Combinatorial Decoding Of Random Nucleic Acid Arrays
  • Multiplex Nucleic Acid Reactions
  • Compositions And Kits For Detection Of A Target Nucleic Acid
  • Self-Encoding Sensor With Microspheres
  • Snap-Back Primers And Detectable Hairpin Structures
  • Arid1a And Ppp2r1a Mutations In Cancer
  • Massive Parallel Method For Decoding Dna And Rna
  • Process For Obtaining Recombined Nucleotide Sequences In Vitro, Libraries Of Sequences And Sequences Thus Obtained
  • Polynucleotide Sequence Assay
  • Invasive Cleavage Of Nucleic Acids
  • Nucleic Acid Detection Using Structured Probes
  • Invasion Assay
  • Process For In Vitro Creation Of Recombinant Polynucleotide Sequences By Oriented Ligation
  • Multiplex Decoding Of Array Sensors With Microspheres
  • Isolation Of Nucleic Acids
  • Dna Sequence With Non-Fluorescent Nucleotide Reversible Terminators And Cleavable Label Modified Nucleotide Terminators
  • Methods For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • Combinatorial Decoding Of Random Nucleic Acid Arrays
  • Reaction Mixtures For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Method Of Detecting Mismatching Regions
  • Methods Of Identifying And Treating Individuals Exhibiting Mutant Kit Protein
  • Combinatorial Fluorescence Energy Transfer Tags And Their Applications For Multiplex Genetic Analyses
  • Snap-Back Primers And Detectable Hairpin Structures
  • Four-Color Dna Sequencing By Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators
  • Methods And Compositions For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Detection Of Target Nucleic Acid Sequence By Pto Cleavage And Extension-Dependent Cleavage
  • Self-Encoding Sensor With Microspheres
  • Self-Encoding Fiber Optic Sensor
  • Detection Of Nucleic Acid Differences Using Combined Endonuclease Cleavage And Ligation Reactions
  • Detection Of Target Nucleic Acid Sequences By Pto Cleavage And Extension Assay
  • Serial Isolation Of Multiple Dna Targets From Stool
  • Methods For Quantitative Amplification And Detection Over A Wide Dynamic Range
  • Soybean Polymorphisms And Methods Of Genotyping
  • Serial Isolation Of Multiple Dna Targets From Stool
  • Method For Quantitating Oligonucleotides
  • Methods Of Detecting An Amplified Nucleic Acid
  • Photocleavable Fluorescent Nucleotides For Dna Sequencing On Chip Constructed By Site-Specific Coupling Chemistry
  • Multiplex Decoding Of Array Sensors With Microspheres
  • Massive Parallel Method For Decoding Dna And Rna
  • Detection Of Target Nucleic Acid Sequence By Pto Cleavage And Extension-Dependent Signaling Oligonucleotide Hybridization Assay
  • Method For Sequential Sequencing Nucleic Acids
  • Detection Of Target Nucleic Acid Sequence On Solid Phase By Pto Cleavage And Extension Using Hcto Assay
  • System And Apparatus For Sequential Processing Of Analytes
  • Methods For Detection Of A Target Nucleic Acid By Forming A Cleavage Structure With A Cleavage Resistant Probe
  • Snap-Back Primers And Detectable Hairpin Structures
  • Multiplex Nucleic Acid Reactions
  • Compositions And Methods For The Detection Of A Nucleic Acid Using A Cleavage Reaction
  • Massive Parallel Method For Decoding Dna And Rna
  • Methods For The Administration Of Iloperidone
  • Methods For Detection Of A Target Nucleic Acid By Capture
  • Nucleic Acid Detection Methods Using Universal Priming
  • Massive Parallel Method For Decoding Dna And Rna
  • Pyrosequencing Methods And Related Compositions
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/7044

    DOI

    http://dx.doi.org/10.1038/7044

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030550270

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10096299


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Archaeoglobus fulgidus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteriophage M13", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Endonucleases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Exodeoxyribonuclease V", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Exodeoxyribonucleases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Leukocytes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Models, Biological", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutagenesis, Insertional", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oligonucleotide Probes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymorphism, Restriction Fragment Length", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pyrococcus furiosus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Spectrometry, Fluorescence", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lyamichev", 
            "givenName": "Victor", 
            "id": "sg:person.0655231011.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655231011.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mast", 
            "givenName": "Andrea L.", 
            "id": "sg:person.0730020314.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730020314.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hall", 
            "givenName": "Jeff G.", 
            "id": "sg:person.0725523477.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725523477.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Prudent", 
            "givenName": "James R.", 
            "id": "sg:person.0616126742.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616126742.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kaiser", 
            "givenName": "Michael W.", 
            "id": "sg:person.01111145407.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111145407.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Takova", 
            "givenName": "Tsetska", 
            "id": "sg:person.015501647677.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015501647677.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kwiatkowski", 
            "givenName": "Robert W.", 
            "id": "sg:person.0722405643.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722405643.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sander", 
            "givenName": "Tamara J.", 
            "id": "sg:person.01274723714.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274723714.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Arruda", 
            "givenName": "Monika", 
            "id": "sg:person.0672540217.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672540217.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Arco", 
            "givenName": "David A.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Neri", 
            "givenName": "Bruce P.", 
            "id": "sg:person.0707573506.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707573506.77"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hologic (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421696.e", 
              "name": [
                "Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brow", 
            "givenName": "Mary Ann D.", 
            "id": "sg:person.0671404235.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671404235.83"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/371432a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007555686", 
              "https://doi.org/10.1038/371432a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/382090a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014222955", 
              "https://doi.org/10.1038/382090a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bies.950190309", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015423625"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.271.47.30068", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016683437"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.7.2674", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026336422"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.8.11.1344", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033818632"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.me.44.020193.001025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034229677"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(82)90404-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036248164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/abio.1995.1320", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046071825"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.7683443", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062648393"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076636181", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078781886", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082740148", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083148073", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1999-03", 
        "datePublishedReg": "1999-03-01", 
        "description": "Flap endonucleases (FENs) isolated from archaea are shown to recognize and cleave a structure formed when two overlapping oligonucleotides hybridize to a target DNA strand. The downstream oligonucleotide probe is cleaved, and the precise site of cleavage is dependent on the amount of overlap with the upstream oligonucleotide. We have demonstrated that use of thermostable archaeal FENs allows the reaction to be performed at temperatures that promote probe turnover without the need for temperature cycling. The resulting amplification of the cleavage signal enables the detection of specific DNA targets at sub-attomole levels within complex mixtures. Moreover, we provide evidence that this cleavage is sufficiently specific to enable discrimination of single-base differences and can differentiate homozygotes from heterozygotes in single-copy genes in genomic DNA.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/7044", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "17"
          }
        ], 
        "name": "Polymorphism identification and quantitative detection of genomic DNA\nby invasive cleavage of oligonucleotide probes", 
        "pagination": "nbt0399_292", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "918972b1175e146528c5d08ebcd1ae5e9a79327af5c97d2b88a49b9a73aa296e"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10096299"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/7044"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1030550270"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/7044", 
          "https://app.dimensions.ai/details/publication/pub.1030550270"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:21", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87078_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt0399_292"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/7044'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/7044'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/7044'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/7044'


     

    This table displays all metadata directly associated to this object as RDF triples.

    237 TRIPLES      21 PREDICATES      56 URIs      34 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/7044 schema:about N010170d864764980849d84be0e39573f
    2 N01709c726bad434ea7606c6e652628dc
    3 N31def43453714d0c930a9960bfe52c4c
    4 N32ef92f284634b0cb7dc22a69c8a82cf
    5 N3a4af20b1a5844d4a4a19087f3109879
    6 N4605383e0b6c43ad8dfec22458b4d078
    7 Naf47a62b210e4b3a873e7c60e493844f
    8 Nbbd57b5487704876bf1c532bb9ebff84
    9 Nbf1a76f2c0db49adb1f43b3b200d346d
    10 Nc1e0c2c4e1a44819afd4cce35e5fafa4
    11 Nd787c852c9324ccba0064e308ce5c27f
    12 Ne1302d3c1cec463b8fe13c250df1cdf7
    13 Ne196b2fbafbc4a2aa296cc0467791fb8
    14 anzsrc-for:06
    15 anzsrc-for:0604
    16 schema:author N414b149812be48a4afed19770e942904
    17 schema:citation sg:pub.10.1038/371432a0
    18 sg:pub.10.1038/382090a0
    19 https://app.dimensions.ai/details/publication/pub.1076636181
    20 https://app.dimensions.ai/details/publication/pub.1078781886
    21 https://app.dimensions.ai/details/publication/pub.1082740148
    22 https://app.dimensions.ai/details/publication/pub.1083148073
    23 https://doi.org/10.1002/bies.950190309
    24 https://doi.org/10.1006/abio.1995.1320
    25 https://doi.org/10.1016/0092-8674(82)90404-4
    26 https://doi.org/10.1073/pnas.91.7.2674
    27 https://doi.org/10.1074/jbc.271.47.30068
    28 https://doi.org/10.1101/gad.8.11.1344
    29 https://doi.org/10.1126/science.7683443
    30 https://doi.org/10.1146/annurev.me.44.020193.001025
    31 schema:datePublished 1999-03
    32 schema:datePublishedReg 1999-03-01
    33 schema:description Flap endonucleases (FENs) isolated from archaea are shown to recognize and cleave a structure formed when two overlapping oligonucleotides hybridize to a target DNA strand. The downstream oligonucleotide probe is cleaved, and the precise site of cleavage is dependent on the amount of overlap with the upstream oligonucleotide. We have demonstrated that use of thermostable archaeal FENs allows the reaction to be performed at temperatures that promote probe turnover without the need for temperature cycling. The resulting amplification of the cleavage signal enables the detection of specific DNA targets at sub-attomole levels within complex mixtures. Moreover, we provide evidence that this cleavage is sufficiently specific to enable discrimination of single-base differences and can differentiate homozygotes from heterozygotes in single-copy genes in genomic DNA.
    34 schema:genre research_article
    35 schema:inLanguage en
    36 schema:isAccessibleForFree false
    37 schema:isPartOf N991d94d51ef74de9ac55c4bddeb41830
    38 N9e24eaf6e47b45a3ac2689a8b2eaed82
    39 sg:journal.1115214
    40 schema:name Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes
    41 schema:pagination nbt0399_292
    42 schema:productId N0e172527e90f4b4db98ffc94e2fe4612
    43 N18314845853d4e05a13710cabf98aca3
    44 N38b9aa4bc48f4278b42ee5730a834f90
    45 N67cb38e7682e42b989fabcb3697dd2e7
    46 Ndf9696f9b494467a9e92ce9b5869861c
    47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030550270
    48 https://doi.org/10.1038/7044
    49 schema:sdDatePublished 2019-04-11T12:21
    50 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    51 schema:sdPublisher N6fda72f4b8f24bdfaa375cc95c3152a3
    52 schema:url http://www.nature.com/articles/nbt0399_292
    53 sgo:license sg:explorer/license/
    54 sgo:sdDataset articles
    55 rdf:type schema:ScholarlyArticle
    56 N010170d864764980849d84be0e39573f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    57 schema:name Oligonucleotide Probes
    58 rdf:type schema:DefinedTerm
    59 N01709c726bad434ea7606c6e652628dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    60 schema:name Bacteriophage M13
    61 rdf:type schema:DefinedTerm
    62 N0e172527e90f4b4db98ffc94e2fe4612 schema:name doi
    63 schema:value 10.1038/7044
    64 rdf:type schema:PropertyValue
    65 N18314845853d4e05a13710cabf98aca3 schema:name readcube_id
    66 schema:value 918972b1175e146528c5d08ebcd1ae5e9a79327af5c97d2b88a49b9a73aa296e
    67 rdf:type schema:PropertyValue
    68 N1d680e2c60264176b169aaaf76c48bea rdf:first sg:person.0616126742.20
    69 rdf:rest N29811de35c7d451b8fb7c5fabf3dae89
    70 N29811de35c7d451b8fb7c5fabf3dae89 rdf:first sg:person.01111145407.02
    71 rdf:rest N6216a8ae63504fedaba2ea97e5428c98
    72 N31def43453714d0c930a9960bfe52c4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    73 schema:name Archaeoglobus fulgidus
    74 rdf:type schema:DefinedTerm
    75 N32ef92f284634b0cb7dc22a69c8a82cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    76 schema:name Endonucleases
    77 rdf:type schema:DefinedTerm
    78 N38988c9814314d38b9b61bed1555949f rdf:first Nf0795be2127446d2bbf4f091b642dfee
    79 rdf:rest Nd22be87428c6424b9ce03982bef9f8e2
    80 N38b9aa4bc48f4278b42ee5730a834f90 schema:name pubmed_id
    81 schema:value 10096299
    82 rdf:type schema:PropertyValue
    83 N3a4af20b1a5844d4a4a19087f3109879 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Exodeoxyribonuclease V
    85 rdf:type schema:DefinedTerm
    86 N414b149812be48a4afed19770e942904 rdf:first sg:person.0655231011.45
    87 rdf:rest Ncedeeb69d6be4f7f8e4952e4df908ff0
    88 N4605383e0b6c43ad8dfec22458b4d078 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Models, Biological
    90 rdf:type schema:DefinedTerm
    91 N4e81f5f6c1e044398d1e6e412fd7292a rdf:first sg:person.0672540217.25
    92 rdf:rest N38988c9814314d38b9b61bed1555949f
    93 N5accf580345e41608e90aa8b50c0ea47 rdf:first sg:person.01274723714.50
    94 rdf:rest N4e81f5f6c1e044398d1e6e412fd7292a
    95 N6216a8ae63504fedaba2ea97e5428c98 rdf:first sg:person.015501647677.04
    96 rdf:rest N6a4a44780524440c9e134369803f9cb5
    97 N67cb38e7682e42b989fabcb3697dd2e7 schema:name nlm_unique_id
    98 schema:value 9604648
    99 rdf:type schema:PropertyValue
    100 N6a4a44780524440c9e134369803f9cb5 rdf:first sg:person.0722405643.72
    101 rdf:rest N5accf580345e41608e90aa8b50c0ea47
    102 N6fda72f4b8f24bdfaa375cc95c3152a3 schema:name Springer Nature - SN SciGraph project
    103 rdf:type schema:Organization
    104 N73ba42ed5878465c9185fc15e4092f72 rdf:first sg:person.0725523477.63
    105 rdf:rest N1d680e2c60264176b169aaaf76c48bea
    106 N991d94d51ef74de9ac55c4bddeb41830 schema:volumeNumber 17
    107 rdf:type schema:PublicationVolume
    108 N9e24eaf6e47b45a3ac2689a8b2eaed82 schema:issueNumber 3
    109 rdf:type schema:PublicationIssue
    110 Naf47a62b210e4b3a873e7c60e493844f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Polymorphism, Restriction Fragment Length
    112 rdf:type schema:DefinedTerm
    113 Nbbd57b5487704876bf1c532bb9ebff84 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name DNA
    115 rdf:type schema:DefinedTerm
    116 Nbf1a76f2c0db49adb1f43b3b200d346d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Pyrococcus furiosus
    118 rdf:type schema:DefinedTerm
    119 Nc1e0c2c4e1a44819afd4cce35e5fafa4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Exodeoxyribonucleases
    121 rdf:type schema:DefinedTerm
    122 Ncedeeb69d6be4f7f8e4952e4df908ff0 rdf:first sg:person.0730020314.53
    123 rdf:rest N73ba42ed5878465c9185fc15e4092f72
    124 Nd22be87428c6424b9ce03982bef9f8e2 rdf:first sg:person.0707573506.77
    125 rdf:rest Nd590420ad5c34f13b76213efbc1db097
    126 Nd590420ad5c34f13b76213efbc1db097 rdf:first sg:person.0671404235.83
    127 rdf:rest rdf:nil
    128 Nd787c852c9324ccba0064e308ce5c27f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Leukocytes
    130 rdf:type schema:DefinedTerm
    131 Ndf9696f9b494467a9e92ce9b5869861c schema:name dimensions_id
    132 schema:value pub.1030550270
    133 rdf:type schema:PropertyValue
    134 Ne1302d3c1cec463b8fe13c250df1cdf7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Mutagenesis, Insertional
    136 rdf:type schema:DefinedTerm
    137 Ne196b2fbafbc4a2aa296cc0467791fb8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Spectrometry, Fluorescence
    139 rdf:type schema:DefinedTerm
    140 Nf0795be2127446d2bbf4f091b642dfee schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    141 schema:familyName Arco
    142 schema:givenName David A.
    143 rdf:type schema:Person
    144 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    145 schema:name Biological Sciences
    146 rdf:type schema:DefinedTerm
    147 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    148 schema:name Genetics
    149 rdf:type schema:DefinedTerm
    150 sg:journal.1115214 schema:issn 1087-0156
    151 1546-1696
    152 schema:name Nature Biotechnology
    153 rdf:type schema:Periodical
    154 sg:person.01111145407.02 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    155 schema:familyName Kaiser
    156 schema:givenName Michael W.
    157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111145407.02
    158 rdf:type schema:Person
    159 sg:person.01274723714.50 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    160 schema:familyName Sander
    161 schema:givenName Tamara J.
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274723714.50
    163 rdf:type schema:Person
    164 sg:person.015501647677.04 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    165 schema:familyName Takova
    166 schema:givenName Tsetska
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015501647677.04
    168 rdf:type schema:Person
    169 sg:person.0616126742.20 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    170 schema:familyName Prudent
    171 schema:givenName James R.
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616126742.20
    173 rdf:type schema:Person
    174 sg:person.0655231011.45 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    175 schema:familyName Lyamichev
    176 schema:givenName Victor
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655231011.45
    178 rdf:type schema:Person
    179 sg:person.0671404235.83 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    180 schema:familyName Brow
    181 schema:givenName Mary Ann D.
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671404235.83
    183 rdf:type schema:Person
    184 sg:person.0672540217.25 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    185 schema:familyName de Arruda
    186 schema:givenName Monika
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672540217.25
    188 rdf:type schema:Person
    189 sg:person.0707573506.77 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    190 schema:familyName Neri
    191 schema:givenName Bruce P.
    192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707573506.77
    193 rdf:type schema:Person
    194 sg:person.0722405643.72 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    195 schema:familyName Kwiatkowski
    196 schema:givenName Robert W.
    197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722405643.72
    198 rdf:type schema:Person
    199 sg:person.0725523477.63 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    200 schema:familyName Hall
    201 schema:givenName Jeff G.
    202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725523477.63
    203 rdf:type schema:Person
    204 sg:person.0730020314.53 schema:affiliation https://www.grid.ac/institutes/grid.421696.e
    205 schema:familyName Mast
    206 schema:givenName Andrea L.
    207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730020314.53
    208 rdf:type schema:Person
    209 sg:pub.10.1038/371432a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007555686
    210 https://doi.org/10.1038/371432a0
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1038/382090a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014222955
    213 https://doi.org/10.1038/382090a0
    214 rdf:type schema:CreativeWork
    215 https://app.dimensions.ai/details/publication/pub.1076636181 schema:CreativeWork
    216 https://app.dimensions.ai/details/publication/pub.1078781886 schema:CreativeWork
    217 https://app.dimensions.ai/details/publication/pub.1082740148 schema:CreativeWork
    218 https://app.dimensions.ai/details/publication/pub.1083148073 schema:CreativeWork
    219 https://doi.org/10.1002/bies.950190309 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015423625
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1006/abio.1995.1320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046071825
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.1016/0092-8674(82)90404-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036248164
    224 rdf:type schema:CreativeWork
    225 https://doi.org/10.1073/pnas.91.7.2674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026336422
    226 rdf:type schema:CreativeWork
    227 https://doi.org/10.1074/jbc.271.47.30068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016683437
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1101/gad.8.11.1344 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033818632
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1126/science.7683443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062648393
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1146/annurev.me.44.020193.001025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034229677
    234 rdf:type schema:CreativeWork
    235 https://www.grid.ac/institutes/grid.421696.e schema:alternateName Hologic (United States)
    236 schema:name Third Wave Technologies, 502 South Rosa Rd., Madison, WI 53719-1256.
    237 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...