Expression profiling using cDNA microarrays View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-01

AUTHORS

D J Duggan, M Bittner, Y Chen, P Meltzer, J M Trent

ABSTRACT

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining. More... »

PAGES

10-14

Journal

TITLE

Nature Genetics

ISSUE

1 Suppl

VOLUME

21

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/4434

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    http://dx.doi.org/10.1038/4434

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9915494


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