Reconstitution of actin-based motility of Listeria and Shigella using pure proteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-10

AUTHORS

Thomas P. Loisel, Rajaa Boujemaa, Dominique Pantaloni, Marie-France Carlier

ABSTRACT

Actin polymerization is essential for cell locomotion and is thought to generate the force responsible for cellular protrusions. The Arp2/3 complex is required to stimulate actin assembly at the leading edge in response to signalling. The bacteria Listeria and Shigella bypass the signalling pathway and harness the Arp2/3 complex to induce actin assembly and to propel themselves in living cells. However, the Arp2/3 complex alone is insufficient to promote movement. Here we have used pure components of the actin cytoskeleton to reconstitute sustained movement in Listeria and Shigella in vitro. Actin-based propulsion is driven by the free energy released by ATP hydrolysis linked to actin polymerization, and does not require myosin. In addition to actin and activated Arp2/3 complex, actin depolymerizing factor (ADF, or cofilin) and capping protein are also required for motility as they maintain a high steady-state level of G-actin, which controls the rate of unidirectional growth of actin filaments at the surface of the bacterium. The movement is more effective when profilin, alpha-actinin and VASP (for Listeria) are also included. These results have implications for our understanding of the mechanism of actin-based motility in cells. More... »

PAGES

613

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/44183

DOI

http://dx.doi.org/10.1038/44183

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035654768

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10524632


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