Reconstitution of actin-based motility of Listeria and Shigella using pure proteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-10

AUTHORS

Thomas P. Loisel, Rajaa Boujemaa, Dominique Pantaloni, Marie-France Carlier

ABSTRACT

Actin polymerization is essential for cell locomotion and is thought to generate the force responsible for cellular protrusions. The Arp2/3 complex is required to stimulate actin assembly at the leading edge in response to signalling. The bacteria Listeria and Shigella bypass the signalling pathway and harness the Arp2/3 complex to induce actin assembly and to propel themselves in living cells. However, the Arp2/3 complex alone is insufficient to promote movement. Here we have used pure components of the actin cytoskeleton to reconstitute sustained movement in Listeria and Shigella in vitro. Actin-based propulsion is driven by the free energy released by ATP hydrolysis linked to actin polymerization, and does not require myosin. In addition to actin and activated Arp2/3 complex, actin depolymerizing factor (ADF, or cofilin) and capping protein are also required for motility as they maintain a high steady-state level of G-actin, which controls the rate of unidirectional growth of actin filaments at the surface of the bacterium. The movement is more effective when profilin, alpha-actinin and VASP (for Listeria) are also included. These results have implications for our understanding of the mechanism of actin-based motility in cells. More... »

PAGES

613

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/44183

DOI

http://dx.doi.org/10.1038/44183

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035654768

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10524632


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actin Depolymerizing Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actin-Related Protein 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actin-Related Protein 3", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cattle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion Molecules", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Contractile Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytoskeletal Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Listeria monocytogenes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microfilament Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nerve Tissue Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Profilins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Shigella", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Wiskott-Aldrich Syndrome Protein, Neuronal", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Laboratoire d'Enzymologie et Biochimie Structurales", 
          "id": "https://www.grid.ac/institutes/grid.420040.2", 
          "name": [
            "Dynamique du Cytosquelette, LEBS, CNRS, Gif-sur-Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Loisel", 
        "givenName": "Thomas P.", 
        "id": "sg:person.01326071070.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326071070.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratoire d'Enzymologie et Biochimie Structurales", 
          "id": "https://www.grid.ac/institutes/grid.420040.2", 
          "name": [
            "Dynamique du Cytosquelette, LEBS, CNRS, Gif-sur-Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boujemaa", 
        "givenName": "Rajaa", 
        "id": "sg:person.01220724016.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220724016.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratoire d'Enzymologie et Biochimie Structurales", 
          "id": "https://www.grid.ac/institutes/grid.420040.2", 
          "name": [
            "Dynamique du Cytosquelette, LEBS, CNRS, Gif-sur-Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pantaloni", 
        "givenName": "Dominique", 
        "id": "sg:person.01243750121.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243750121.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratoire d'Enzymologie et Biochimie Structurales", 
          "id": "https://www.grid.ac/institutes/grid.420040.2", 
          "name": [
            "Dynamique du Cytosquelette, LEBS, CNRS, Gif-sur-Yvette, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Carlier", 
        "givenName": "Marie-France", 
        "id": "sg:person.0776555474.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776555474.73"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1083/jcb.135.3.647", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000013639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.130.2.331", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001896730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/17.10.2767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005234028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(00)88939-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009283696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.146.6.1319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010536568"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.146.6.1319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010536568"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.7.3739", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011319079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(99)80218-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013713613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/34208", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014134258", 
          "https://doi.org/10.1038/34208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/34208", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014134258", 
          "https://doi.org/10.1038/34208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(98)00015-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015776418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016740254", 
          "https://doi.org/10.1038/13040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016740254", 
          "https://doi.org/10.1038/13040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.144.6.1245", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016879206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0169(1997)37:1<44::aid-cm5>3.0.co;2-h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019942998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/385265a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021570808", 
          "https://doi.org/10.1038/385265a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.145.5.1009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021675451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(98)80085-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025244825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90080-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027822662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/16.7.1531", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033870538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.129.1.147", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034014300"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.30.20970", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035778010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.40.25602", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038663322"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1997.1062", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038674842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.136.6.1307", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042114642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1995.mmi_18030413.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045949963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80732-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046826381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.14.6572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048020977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/357257a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050003624", 
          "https://doi.org/10.1038/357257a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(99)80243-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051217848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cm.970280408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052343531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi970601b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055213803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi970601b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055213803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1848726", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062509922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.281.5373.105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062561595"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-10", 
    "datePublishedReg": "1999-10-01", 
    "description": "Actin polymerization is essential for cell locomotion and is thought to generate the force responsible for cellular protrusions. The Arp2/3 complex is required to stimulate actin assembly at the leading edge in response to signalling. The bacteria Listeria and Shigella bypass the signalling pathway and harness the Arp2/3 complex to induce actin assembly and to propel themselves in living cells. However, the Arp2/3 complex alone is insufficient to promote movement. Here we have used pure components of the actin cytoskeleton to reconstitute sustained movement in Listeria and Shigella in vitro. Actin-based propulsion is driven by the free energy released by ATP hydrolysis linked to actin polymerization, and does not require myosin. In addition to actin and activated Arp2/3 complex, actin depolymerizing factor (ADF, or cofilin) and capping protein are also required for motility as they maintain a high steady-state level of G-actin, which controls the rate of unidirectional growth of actin filaments at the surface of the bacterium. The movement is more effective when profilin, alpha-actinin and VASP (for Listeria) are also included. These results have implications for our understanding of the mechanism of actin-based motility in cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/44183", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6753", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "401"
      }
    ], 
    "name": "Reconstitution of actin-based motility of Listeria and Shigella using pure proteins", 
    "pagination": "613", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a9d8c65dfc510df43b78cf29f580f4d5da4920883c4b9c2052a766f831058211"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10524632"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/44183"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1035654768"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/44183", 
      "https://app.dimensions.ai/details/publication/pub.1035654768"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:11", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53984_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/44183"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/44183'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/44183'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/44183'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/44183'


 

This table displays all metadata directly associated to this object as RDF triples.

287 TRIPLES      21 PREDICATES      85 URIs      46 LITERALS      34 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/44183 schema:about N06fcec8e5ecc4b3e97cd59a81ae1703d
2 N0c59c859c52249a594ad305abe93a214
3 N17b2944259c34bb6aca771437548b3f9
4 N25a5f7e381d942709166577e07adb7ba
5 N2b392ce094ea469fa9d1d591b654f4a1
6 N332ec8416f9e4f25bf0782607cc243af
7 N3a93e3ed12db4cf7bbac658fd5ed6499
8 N5146b91eb99b4b2193a532c48026624b
9 N745f795464da44409ce37fb06636afa3
10 N82271f115ad44816969c8896a5a54bd1
11 N84a89ceeffa841d8943a1d7508ffe6b7
12 N877a8ff85d134dd683e3b3ca6f879eaf
13 N87a04fddfd124fafa93aba1249074952
14 N8867a60f78f04b868d15175d495fd968
15 N8a9434692f9a4abc9f7d6cddc9dc5887
16 N9153cb49fd8b4bcba86a9a2b5478292a
17 Na1f5c6ba2ab946bbac02a58f695c23c9
18 Nb7449fc264f64765a4909632ade0887a
19 Ndda54670c0af40d2b33beba5815bf69e
20 Ne77cf6331670464697601bcbd837410a
21 Nf22198feb06745f1b023f13193885dff
22 Nf2ce671ee303469ba23868c5f1373dd4
23 Nf7da019acb4044878343c4e0ff3b8fac
24 Nfbad545f667c48d6aca1a55019ef7086
25 Nfc2e336a71cc4d80b38dc3cffbebcc92
26 anzsrc-for:06
27 anzsrc-for:0601
28 schema:author N36fba4782dee433fbe3985221dea946b
29 schema:citation sg:pub.10.1038/13040
30 sg:pub.10.1038/34208
31 sg:pub.10.1038/357257a0
32 sg:pub.10.1038/385265a0
33 https://doi.org/10.1002/(sici)1097-0169(1997)37:1<44::aid-cm5>3.0.co;2-h
34 https://doi.org/10.1002/cm.970280408
35 https://doi.org/10.1006/jmbi.1997.1062
36 https://doi.org/10.1016/0092-8674(95)90080-2
37 https://doi.org/10.1016/s0092-8674(00)80732-1
38 https://doi.org/10.1016/s0955-0674(98)80085-9
39 https://doi.org/10.1016/s0960-9822(98)00015-3
40 https://doi.org/10.1016/s0960-9822(99)80218-8
41 https://doi.org/10.1016/s0960-9822(99)80243-7
42 https://doi.org/10.1016/s0962-8924(00)88939-4
43 https://doi.org/10.1021/bi970601b
44 https://doi.org/10.1073/pnas.92.14.6572
45 https://doi.org/10.1073/pnas.96.7.3739
46 https://doi.org/10.1074/jbc.273.40.25602
47 https://doi.org/10.1074/jbc.274.30.20970
48 https://doi.org/10.1083/jcb.129.1.147
49 https://doi.org/10.1083/jcb.130.2.331
50 https://doi.org/10.1083/jcb.135.3.647
51 https://doi.org/10.1083/jcb.136.6.1307
52 https://doi.org/10.1083/jcb.144.6.1245
53 https://doi.org/10.1083/jcb.145.5.1009
54 https://doi.org/10.1083/jcb.146.6.1319
55 https://doi.org/10.1093/emboj/16.7.1531
56 https://doi.org/10.1093/emboj/17.10.2767
57 https://doi.org/10.1111/j.1365-2958.1995.mmi_18030413.x
58 https://doi.org/10.1126/science.1848726
59 https://doi.org/10.1126/science.281.5373.105
60 schema:datePublished 1999-10
61 schema:datePublishedReg 1999-10-01
62 schema:description Actin polymerization is essential for cell locomotion and is thought to generate the force responsible for cellular protrusions. The Arp2/3 complex is required to stimulate actin assembly at the leading edge in response to signalling. The bacteria Listeria and Shigella bypass the signalling pathway and harness the Arp2/3 complex to induce actin assembly and to propel themselves in living cells. However, the Arp2/3 complex alone is insufficient to promote movement. Here we have used pure components of the actin cytoskeleton to reconstitute sustained movement in Listeria and Shigella in vitro. Actin-based propulsion is driven by the free energy released by ATP hydrolysis linked to actin polymerization, and does not require myosin. In addition to actin and activated Arp2/3 complex, actin depolymerizing factor (ADF, or cofilin) and capping protein are also required for motility as they maintain a high steady-state level of G-actin, which controls the rate of unidirectional growth of actin filaments at the surface of the bacterium. The movement is more effective when profilin, alpha-actinin and VASP (for Listeria) are also included. These results have implications for our understanding of the mechanism of actin-based motility in cells.
63 schema:genre research_article
64 schema:inLanguage en
65 schema:isAccessibleForFree false
66 schema:isPartOf N37baea937809435ca40faf9dc9ea2246
67 Nd4d81c156de04ef6b3f38f4cdb6e088e
68 sg:journal.1018957
69 schema:name Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
70 schema:pagination 613
71 schema:productId N6d8138d013bd4bc3858059ae0f403442
72 N8b4b34f1dd9d476e8844e10d27f499ea
73 N9e71858b0007479cb477aaba36cd4352
74 Ned274bdcd6064929bbd05c0fa65509c0
75 Nfc30bda903264243928d560b2d4d6bee
76 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035654768
77 https://doi.org/10.1038/44183
78 schema:sdDatePublished 2019-04-11T12:11
79 schema:sdLicense https://scigraph.springernature.com/explorer/license/
80 schema:sdPublisher Naebfecca233c4005b41153017ef00bb9
81 schema:url https://www.nature.com/articles/44183
82 sgo:license sg:explorer/license/
83 sgo:sdDataset articles
84 rdf:type schema:ScholarlyArticle
85 N06fcec8e5ecc4b3e97cd59a81ae1703d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Escherichia coli
87 rdf:type schema:DefinedTerm
88 N0c59c859c52249a594ad305abe93a214 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Profilins
90 rdf:type schema:DefinedTerm
91 N17b2944259c34bb6aca771437548b3f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name DNA-Binding Proteins
93 rdf:type schema:DefinedTerm
94 N199486517f024fc0845f12b94239cade rdf:first sg:person.0776555474.73
95 rdf:rest rdf:nil
96 N25a5f7e381d942709166577e07adb7ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Movement
98 rdf:type schema:DefinedTerm
99 N2b392ce094ea469fa9d1d591b654f4a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Cytoskeletal Proteins
101 rdf:type schema:DefinedTerm
102 N332ec8416f9e4f25bf0782607cc243af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Actin Depolymerizing Factors
104 rdf:type schema:DefinedTerm
105 N36fba4782dee433fbe3985221dea946b rdf:first sg:person.01326071070.09
106 rdf:rest N4bc22025514d4b41955064ddc572af30
107 N37baea937809435ca40faf9dc9ea2246 schema:issueNumber 6753
108 rdf:type schema:PublicationIssue
109 N3a93e3ed12db4cf7bbac658fd5ed6499 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Contractile Proteins
111 rdf:type schema:DefinedTerm
112 N4bc22025514d4b41955064ddc572af30 rdf:first sg:person.01220724016.91
113 rdf:rest N935a287aba8349469c214aa16a4d1b35
114 N5146b91eb99b4b2193a532c48026624b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Cell Adhesion Molecules
116 rdf:type schema:DefinedTerm
117 N6d8138d013bd4bc3858059ae0f403442 schema:name nlm_unique_id
118 schema:value 0410462
119 rdf:type schema:PropertyValue
120 N745f795464da44409ce37fb06636afa3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Actin-Related Protein 3
122 rdf:type schema:DefinedTerm
123 N82271f115ad44816969c8896a5a54bd1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Actins
125 rdf:type schema:DefinedTerm
126 N84a89ceeffa841d8943a1d7508ffe6b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Recombinant Proteins
128 rdf:type schema:DefinedTerm
129 N877a8ff85d134dd683e3b3ca6f879eaf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Shigella
131 rdf:type schema:DefinedTerm
132 N87a04fddfd124fafa93aba1249074952 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Transcription Factors
134 rdf:type schema:DefinedTerm
135 N8867a60f78f04b868d15175d495fd968 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Humans
137 rdf:type schema:DefinedTerm
138 N8a9434692f9a4abc9f7d6cddc9dc5887 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Adenosine Triphosphate
140 rdf:type schema:DefinedTerm
141 N8b4b34f1dd9d476e8844e10d27f499ea schema:name pubmed_id
142 schema:value 10524632
143 rdf:type schema:PropertyValue
144 N9153cb49fd8b4bcba86a9a2b5478292a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Microfilament Proteins
146 rdf:type schema:DefinedTerm
147 N935a287aba8349469c214aa16a4d1b35 rdf:first sg:person.01243750121.06
148 rdf:rest N199486517f024fc0845f12b94239cade
149 N9e71858b0007479cb477aaba36cd4352 schema:name dimensions_id
150 schema:value pub.1035654768
151 rdf:type schema:PropertyValue
152 Na1f5c6ba2ab946bbac02a58f695c23c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Cattle
154 rdf:type schema:DefinedTerm
155 Naebfecca233c4005b41153017ef00bb9 schema:name Springer Nature - SN SciGraph project
156 rdf:type schema:Organization
157 Nb7449fc264f64765a4909632ade0887a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Nerve Tissue Proteins
159 rdf:type schema:DefinedTerm
160 Nd4d81c156de04ef6b3f38f4cdb6e088e schema:volumeNumber 401
161 rdf:type schema:PublicationVolume
162 Ndda54670c0af40d2b33beba5815bf69e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Phosphoproteins
164 rdf:type schema:DefinedTerm
165 Ne77cf6331670464697601bcbd837410a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Wiskott-Aldrich Syndrome Protein, Neuronal
167 rdf:type schema:DefinedTerm
168 Ned274bdcd6064929bbd05c0fa65509c0 schema:name doi
169 schema:value 10.1038/44183
170 rdf:type schema:PropertyValue
171 Nf22198feb06745f1b023f13193885dff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Rabbits
173 rdf:type schema:DefinedTerm
174 Nf2ce671ee303469ba23868c5f1373dd4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Listeria monocytogenes
176 rdf:type schema:DefinedTerm
177 Nf7da019acb4044878343c4e0ff3b8fac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Bacterial Proteins
179 rdf:type schema:DefinedTerm
180 Nfbad545f667c48d6aca1a55019ef7086 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Actin-Related Protein 2
182 rdf:type schema:DefinedTerm
183 Nfc2e336a71cc4d80b38dc3cffbebcc92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Animals
185 rdf:type schema:DefinedTerm
186 Nfc30bda903264243928d560b2d4d6bee schema:name readcube_id
187 schema:value a9d8c65dfc510df43b78cf29f580f4d5da4920883c4b9c2052a766f831058211
188 rdf:type schema:PropertyValue
189 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
190 schema:name Biological Sciences
191 rdf:type schema:DefinedTerm
192 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
193 schema:name Biochemistry and Cell Biology
194 rdf:type schema:DefinedTerm
195 sg:journal.1018957 schema:issn 0090-0028
196 1476-4687
197 schema:name Nature
198 rdf:type schema:Periodical
199 sg:person.01220724016.91 schema:affiliation https://www.grid.ac/institutes/grid.420040.2
200 schema:familyName Boujemaa
201 schema:givenName Rajaa
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220724016.91
203 rdf:type schema:Person
204 sg:person.01243750121.06 schema:affiliation https://www.grid.ac/institutes/grid.420040.2
205 schema:familyName Pantaloni
206 schema:givenName Dominique
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243750121.06
208 rdf:type schema:Person
209 sg:person.01326071070.09 schema:affiliation https://www.grid.ac/institutes/grid.420040.2
210 schema:familyName Loisel
211 schema:givenName Thomas P.
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326071070.09
213 rdf:type schema:Person
214 sg:person.0776555474.73 schema:affiliation https://www.grid.ac/institutes/grid.420040.2
215 schema:familyName Carlier
216 schema:givenName Marie-France
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776555474.73
218 rdf:type schema:Person
219 sg:pub.10.1038/13040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016740254
220 https://doi.org/10.1038/13040
221 rdf:type schema:CreativeWork
222 sg:pub.10.1038/34208 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014134258
223 https://doi.org/10.1038/34208
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/357257a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050003624
226 https://doi.org/10.1038/357257a0
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/385265a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021570808
229 https://doi.org/10.1038/385265a0
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1002/(sici)1097-0169(1997)37:1<44::aid-cm5>3.0.co;2-h schema:sameAs https://app.dimensions.ai/details/publication/pub.1019942998
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1002/cm.970280408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052343531
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1006/jmbi.1997.1062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038674842
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/0092-8674(95)90080-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027822662
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/s0092-8674(00)80732-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046826381
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/s0955-0674(98)80085-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025244825
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/s0960-9822(98)00015-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015776418
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/s0960-9822(99)80218-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013713613
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/s0960-9822(99)80243-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051217848
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/s0962-8924(00)88939-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009283696
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1021/bi970601b schema:sameAs https://app.dimensions.ai/details/publication/pub.1055213803
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1073/pnas.92.14.6572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048020977
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1073/pnas.96.7.3739 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011319079
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1074/jbc.273.40.25602 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038663322
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1074/jbc.274.30.20970 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035778010
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1083/jcb.129.1.147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034014300
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1083/jcb.130.2.331 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001896730
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1083/jcb.135.3.647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000013639
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1083/jcb.136.6.1307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042114642
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1083/jcb.144.6.1245 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016879206
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1083/jcb.145.5.1009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021675451
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1083/jcb.146.6.1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010536568
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1093/emboj/16.7.1531 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033870538
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1093/emboj/17.10.2767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005234028
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1111/j.1365-2958.1995.mmi_18030413.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1045949963
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1126/science.1848726 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062509922
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1126/science.281.5373.105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062561595
284 rdf:type schema:CreativeWork
285 https://www.grid.ac/institutes/grid.420040.2 schema:alternateName Laboratoire d'Enzymologie et Biochimie Structurales
286 schema:name Dynamique du Cytosquelette, LEBS, CNRS, Gif-sur-Yvette, France
287 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...