Ontology type: schema:ScholarlyArticle
1997-04
AUTHORSS L Dove, J K Joung, A Hochschild
ABSTRACTMany transcriptional activators in prokaryotes are known to bind near a promoter and contact RNA polymerase, but it is not clear whether a protein-protein contact between an activator and RNA polymerase is enough to activate gene transcription. Here we show that contact between a DNA-bound protein and a heterologous protein domain fused to RNA polymerase can elicit transcriptional activation; moreover, the strength of this engineered protein-protein interaction determines the amount of gene activation. Our results indicate that an arbitrary interaction between a DNA-bound protein and RNA polymerase can activate transcription. We also find that when the DNA-bound 'activator' makes contact with two different components of the polymerase, the effect of these two interactions on transcription is synergistic. More... »
PAGES627-630
http://scigraph.springernature.com/pub.10.1038/386627a0
DOIhttp://dx.doi.org/10.1038/386627a0
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