CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-11

AUTHORS

L Wu, N P Gerard, R Wyatt, H Choe, C Parolin, N Ruffing, A Borsetti, A A Cardoso, E Desjardin, W Newman, C Gerard, J Sodroski

ABSTRACT

For efficient entry into target cells, primary macrophage-tropic and laboratory-adapted human immunodeficiency viruses type 1 (HIV-1) require particular chemokine receptors, CCR-5 and CXCR-4, respectively, as well as the primary receptor CD4 (refs 1-6). Here we show that a complex of gp120, the exterior envelope glycoprotein, of macrophage-tropic primary HIV-1 and soluble CD4 interacts specifically with CCR-5 and inhibits the binding of the natural CCR-5 ligands, macrophage inflammatory protein (MIP)-1alpha and MIP-1beta (refs 7, 8). The apparent affinity of the interaction between gp120 and CCR-5 was dramatically lower in the absence of soluble CD4. Additionally, in the absence of gp120, an interaction between a two-domain CD4 fragment and CCR-5 was observed. A gp120 fragment retaining the CD4-binding site and overlapping epitopes was able to interact with CCR-5 only if the V3 loop, which can specify HIV-1 tropism and chemokine receptor choice, was also present on the molecule. Neutralizing antibodies directed against either CD4-induced or V3 epitopes on gp120 blocked the interaction of gp12O-CD4 complexes with CCR-5. These results suggest that HIV-1 attachment to CD4 creates a high-affinity binding site for CCR-5, leading to membrane fusion and virus entry. More... »

PAGES

179-183

Journal

TITLE

Nature

ISSUE

6605

VOLUME

384

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/384179a0

    DOI

    http://dx.doi.org/10.1038/384179a0

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8906795


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