Movement and force produced by a single myosin head View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-11

AUTHORS

J E Molloy, J E Burns, J Kendrick-Jones, R T Tregear, D C White

ABSTRACT

Muscle contraction is driven by the cyclical interaction of myosin with actin, coupled to the breakdown of ATP. Studies of the interaction of filamentous myosin and of a double-headed proteolytic fragment, heavy meromyosin (HMM), with actin have demonstrated discrete mechanical events, arising from stochastic interaction of single myosin molecules with actin. Here we show, using an optical-tweezers transducer, that a single myosin subfragment-1 (S1), which is a single myosin head, can act as an independent generator of force and movement. Our analysis accounts for the broad distribution of displacement amplitudes observed, and indicates that the underlying movement (working stroke) produced by a single acto-S1 interaction is approximately 4 nm, considerably shorter than previous estimates but consistent with structural data. We measure the average force generated by S1 or HMM to be at least 1.7 pN under isometric conditions. More... »

PAGES

209-212

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/378209a0

DOI

http://dx.doi.org/10.1038/378209a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031755821

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7477328


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Other Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomechanical Phenomena", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myosin Subfragments", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Optics and Photonics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of York", 
          "id": "https://www.grid.ac/institutes/grid.5685.e", 
          "name": [
            "Department of Biology, University of York, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Molloy", 
        "givenName": "J E", 
        "id": "sg:person.01231251602.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231251602.87"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Burns", 
        "givenName": "J E", 
        "id": "sg:person.01105277330.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105277330.57"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Kendrick-Jones", 
        "givenName": "J", 
        "id": "sg:person.01213765401.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213765401.81"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Tregear", 
        "givenName": "R T", 
        "id": "sg:person.01304201377.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304201377.55"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "White", 
        "givenName": "D C", 
        "id": "sg:person.01335754330.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335754330.59"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0076-6879(91)96035-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002822964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1994.1336", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007459572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/365721a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026078609", 
          "https://doi.org/10.1038/365721a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/326805a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040356440", 
          "https://doi.org/10.1038/326805a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/328536a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048739855", 
          "https://doi.org/10.1038/328536a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/368113a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049237121", 
          "https://doi.org/10.1038/368113a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/233533a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050599860", 
          "https://doi.org/10.1038/233533a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/233533a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050599860", 
          "https://doi.org/10.1038/233533a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/334074a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050990343", 
          "https://doi.org/10.1038/334074a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8316857", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062654660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082507351", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1995-11", 
    "datePublishedReg": "1995-11-01", 
    "description": "Muscle contraction is driven by the cyclical interaction of myosin with actin, coupled to the breakdown of ATP. Studies of the interaction of filamentous myosin and of a double-headed proteolytic fragment, heavy meromyosin (HMM), with actin have demonstrated discrete mechanical events, arising from stochastic interaction of single myosin molecules with actin. Here we show, using an optical-tweezers transducer, that a single myosin subfragment-1 (S1), which is a single myosin head, can act as an independent generator of force and movement. Our analysis accounts for the broad distribution of displacement amplitudes observed, and indicates that the underlying movement (working stroke) produced by a single acto-S1 interaction is approximately 4 nm, considerably shorter than previous estimates but consistent with structural data. We measure the average force generated by S1 or HMM to be at least 1.7 pN under isometric conditions.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/378209a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6553", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "378"
      }
    ], 
    "name": "Movement and force produced by a single myosin head", 
    "pagination": "209-212", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "97472ec9dfe9b34ae56d890b9221fabac20fa23f2d416587e3303a9c37154a2d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7477328"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/378209a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1031755821"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/378209a0", 
      "https://app.dimensions.ai/details/publication/pub.1031755821"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T00:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000425.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/378209a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/378209a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/378209a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/378209a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/378209a0'


 

This table displays all metadata directly associated to this object as RDF triples.

160 TRIPLES      21 PREDICATES      47 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/378209a0 schema:about N1590c5771456420baaf7144f22f36440
2 N5cdbae3e3a4b4d9d91f9ece27dd90355
3 N5d77f5525e9847eca37e62742863a09b
4 N784518651fed4cd792d97d6dfd785661
5 Nd8a142d6749b4e13a735d6304f412ef1
6 Ne39e9f81cd6c460fac06e42820faf302
7 Nf7b3fbb08aec492d9a889dee571f18bd
8 Nfbeb8a4d38214aa08b29d7bcb0bf7260
9 anzsrc-for:02
10 anzsrc-for:0299
11 schema:author N28f36a34c56e45b69da34611e9ed118f
12 schema:citation sg:pub.10.1038/233533a0
13 sg:pub.10.1038/326805a0
14 sg:pub.10.1038/328536a0
15 sg:pub.10.1038/334074a0
16 sg:pub.10.1038/365721a0
17 sg:pub.10.1038/368113a0
18 https://app.dimensions.ai/details/publication/pub.1082507351
19 https://doi.org/10.1006/bbrc.1994.1336
20 https://doi.org/10.1016/0076-6879(91)96035-p
21 https://doi.org/10.1126/science.8316857
22 schema:datePublished 1995-11
23 schema:datePublishedReg 1995-11-01
24 schema:description Muscle contraction is driven by the cyclical interaction of myosin with actin, coupled to the breakdown of ATP. Studies of the interaction of filamentous myosin and of a double-headed proteolytic fragment, heavy meromyosin (HMM), with actin have demonstrated discrete mechanical events, arising from stochastic interaction of single myosin molecules with actin. Here we show, using an optical-tweezers transducer, that a single myosin subfragment-1 (S1), which is a single myosin head, can act as an independent generator of force and movement. Our analysis accounts for the broad distribution of displacement amplitudes observed, and indicates that the underlying movement (working stroke) produced by a single acto-S1 interaction is approximately 4 nm, considerably shorter than previous estimates but consistent with structural data. We measure the average force generated by S1 or HMM to be at least 1.7 pN under isometric conditions.
25 schema:genre research_article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf N8ea57149e2ee42d890b0eeaad042258a
29 Ncc40c516f44b45b5a3faaeef2095e04c
30 sg:journal.1018957
31 schema:name Movement and force produced by a single myosin head
32 schema:pagination 209-212
33 schema:productId N08f81ad42abd4dc09668840e03fcc3b5
34 N67c06986fd2b4cd9ae7a24e14ed4e24c
35 N763ad205462749ca9837ab29db40e5a6
36 Na000f7c3801b458c9e4e17f2844ef3e4
37 Nab8a861f8cfd4457837eef90b4d12d06
38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031755821
39 https://doi.org/10.1038/378209a0
40 schema:sdDatePublished 2019-04-11T00:55
41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
42 schema:sdPublisher N8846e06d19f64ec7b77dc33b3d5c0b22
43 schema:url http://www.nature.com/articles/378209a0
44 sgo:license sg:explorer/license/
45 sgo:sdDataset articles
46 rdf:type schema:ScholarlyArticle
47 N08f81ad42abd4dc09668840e03fcc3b5 schema:name pubmed_id
48 schema:value 7477328
49 rdf:type schema:PropertyValue
50 N1590c5771456420baaf7144f22f36440 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Biomechanical Phenomena
52 rdf:type schema:DefinedTerm
53 N28f36a34c56e45b69da34611e9ed118f rdf:first sg:person.01231251602.87
54 rdf:rest N3b12d2e30aaa4b55a38916d74e5311b6
55 N32577fb97c4342b28c55c4c1805ccafe rdf:first sg:person.01335754330.59
56 rdf:rest rdf:nil
57 N3b12d2e30aaa4b55a38916d74e5311b6 rdf:first sg:person.01105277330.57
58 rdf:rest N51dc42dd1b2241f6ba203a54b7e3fd67
59 N51dc42dd1b2241f6ba203a54b7e3fd67 rdf:first sg:person.01213765401.81
60 rdf:rest Ne2555770992245eab213c51f98b977f0
61 N5cdbae3e3a4b4d9d91f9ece27dd90355 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Actins
63 rdf:type schema:DefinedTerm
64 N5d77f5525e9847eca37e62742863a09b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Animals
66 rdf:type schema:DefinedTerm
67 N67c06986fd2b4cd9ae7a24e14ed4e24c schema:name readcube_id
68 schema:value 97472ec9dfe9b34ae56d890b9221fabac20fa23f2d416587e3303a9c37154a2d
69 rdf:type schema:PropertyValue
70 N763ad205462749ca9837ab29db40e5a6 schema:name doi
71 schema:value 10.1038/378209a0
72 rdf:type schema:PropertyValue
73 N784518651fed4cd792d97d6dfd785661 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Movement
75 rdf:type schema:DefinedTerm
76 N8846e06d19f64ec7b77dc33b3d5c0b22 schema:name Springer Nature - SN SciGraph project
77 rdf:type schema:Organization
78 N8ea57149e2ee42d890b0eeaad042258a schema:issueNumber 6553
79 rdf:type schema:PublicationIssue
80 Na000f7c3801b458c9e4e17f2844ef3e4 schema:name dimensions_id
81 schema:value pub.1031755821
82 rdf:type schema:PropertyValue
83 Nab8a861f8cfd4457837eef90b4d12d06 schema:name nlm_unique_id
84 schema:value 0410462
85 rdf:type schema:PropertyValue
86 Ncc40c516f44b45b5a3faaeef2095e04c schema:volumeNumber 378
87 rdf:type schema:PublicationVolume
88 Nd8a142d6749b4e13a735d6304f412ef1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name In Vitro Techniques
90 rdf:type schema:DefinedTerm
91 Ne2555770992245eab213c51f98b977f0 rdf:first sg:person.01304201377.55
92 rdf:rest N32577fb97c4342b28c55c4c1805ccafe
93 Ne39e9f81cd6c460fac06e42820faf302 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Rabbits
95 rdf:type schema:DefinedTerm
96 Nf7b3fbb08aec492d9a889dee571f18bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Optics and Photonics
98 rdf:type schema:DefinedTerm
99 Nfbeb8a4d38214aa08b29d7bcb0bf7260 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Myosin Subfragments
101 rdf:type schema:DefinedTerm
102 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
103 schema:name Physical Sciences
104 rdf:type schema:DefinedTerm
105 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
106 schema:name Other Physical Sciences
107 rdf:type schema:DefinedTerm
108 sg:journal.1018957 schema:issn 0090-0028
109 1476-4687
110 schema:name Nature
111 rdf:type schema:Periodical
112 sg:person.01105277330.57 schema:familyName Burns
113 schema:givenName J E
114 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105277330.57
115 rdf:type schema:Person
116 sg:person.01213765401.81 schema:familyName Kendrick-Jones
117 schema:givenName J
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213765401.81
119 rdf:type schema:Person
120 sg:person.01231251602.87 schema:affiliation https://www.grid.ac/institutes/grid.5685.e
121 schema:familyName Molloy
122 schema:givenName J E
123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231251602.87
124 rdf:type schema:Person
125 sg:person.01304201377.55 schema:familyName Tregear
126 schema:givenName R T
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304201377.55
128 rdf:type schema:Person
129 sg:person.01335754330.59 schema:familyName White
130 schema:givenName D C
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335754330.59
132 rdf:type schema:Person
133 sg:pub.10.1038/233533a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050599860
134 https://doi.org/10.1038/233533a0
135 rdf:type schema:CreativeWork
136 sg:pub.10.1038/326805a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040356440
137 https://doi.org/10.1038/326805a0
138 rdf:type schema:CreativeWork
139 sg:pub.10.1038/328536a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048739855
140 https://doi.org/10.1038/328536a0
141 rdf:type schema:CreativeWork
142 sg:pub.10.1038/334074a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050990343
143 https://doi.org/10.1038/334074a0
144 rdf:type schema:CreativeWork
145 sg:pub.10.1038/365721a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026078609
146 https://doi.org/10.1038/365721a0
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/368113a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049237121
149 https://doi.org/10.1038/368113a0
150 rdf:type schema:CreativeWork
151 https://app.dimensions.ai/details/publication/pub.1082507351 schema:CreativeWork
152 https://doi.org/10.1006/bbrc.1994.1336 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007459572
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1016/0076-6879(91)96035-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1002822964
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1126/science.8316857 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062654660
157 rdf:type schema:CreativeWork
158 https://www.grid.ac/institutes/grid.5685.e schema:alternateName University of York
159 schema:name Department of Biology, University of York, UK.
160 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...