Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-04

AUTHORS

Takashi Funatsu, Yoshie Harada, Makio Tokunaga, Kiwamu Saito, Toshio Yanagida

ABSTRACT

VISUALIZATION of single actin filaments by fluorescence microscopy1led to the development of new in vitro assays for analysing actomyosin-based motility at the molecular level2-5. The ability to manipulate actin filaments with a microneedle6,7 or an optical trap8 combined with position-sensitive detectors has enabled direct measurements of nanometre displacements and piconewton forces exerted by individual myosin molecules. To elucidate how myosin generates movement, it is necessary to understand how ATP hydrolysis is coupled to mechanical work at the level of the single molecule. But the most sensitive microscopic ATPase assay available still requires over 1,000 myosins9. To enhance the sensitivity of such assays, we have refined epifluorescence and total internal reflection microscopies to visualize single fluorescent dye molecules. We report here that this approach can be used directly to image single fluorescently labelled myosin molecules and detect individual ATP turnover reactions. In contrast to previously reported single fluorescent molecule imaging methods, which used specimens immobilized on an air-dried surface10-12, our method allows video-rate imaging of single molecules in aqueous solution, and hence can be applied to the study of many types of enzymes and biomolecules. More... »

PAGES

555-559

Journal

TITLE

Nature

ISSUE

6522

VOLUME

374

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/374555a0

    DOI

    http://dx.doi.org/10.1038/374555a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006208507

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7700383


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