CpG motifs in bacterial DNA trigger direct B-cell activation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-04

AUTHORS

Arthur M. Krieg, Ae-Kyung Yi, Sara Matson, Thomas J. Waldschmidt, Gail A. Bishop, Rebecca Teasdale, Gary A. Koretzky, Dennis M. Klinman

ABSTRACT

UNMETHYLATED CpG dinucleotides are more frequent in the gen-omes of bacteria and viruses than of vertebrates. We report here that bacterial DNA and synthetic oligodeoxynucleotides containing unmethylated CpG dinucleotides induce murine B cells to proliferate and secrete immunoglobulin in vitro and in vivo. This activation is enhanced by simultaneous signals delivered through the antigen receptor. Optimal B-cell activation requires a DNA motif in which an unmethylated CpG dinucleotide is flanked by two 5' purines and two 3' pyrimidines. Oligodeoxynucleotides containing this CpG motif induce more than 95% of all spleen B cells to enter the cell cycle. These data suggest a possible evolutionary link between immune defence based on the recognition of microbial DNA and the phenomenon of 'CpG suppression' in vertebrates. The potent immune activation by CpG oligonucleotides has impli-cations for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants. More... »

PAGES

546-549

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

6522

VOLUME

374

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  • Identifiers

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