Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-03

AUTHORS

Jenny E. Hinshaw, Sandra L. Schmid

ABSTRACT

DYNAMIN, a 100K member of the GTPase superfamily1, is the mammalian homologue of the Drosophila shibire gene product2,3. Mutations in shibire cause a defect in endocytosis leading to accumulation of coated pits and deep imaginations at the plasma membrane of all tissues examined4,5. Similarly, invaginated coated pits accumulate in mammalian cells overexpressing dominant-negative mutants of dynamin, establishing that dynamin is required for the formation of 'constricted' coated pits and for coated vesicle budding6. Whether dynamin functions in the classic GTPase mode as a molecular switch to regulate events leading to coated vesicle budding or instead actively participates as a mechanochemical enzyme driving coated vesicle formation is unclear7. Here we show that dynamin spontaneously self-assembles into rings and stacks of interconnected rings, comparable in dimension to the 'collars' observed at the necks of invaginated coated pits that accumulate at synaptic terminals in shibire flies4. We propose that invaginated coated pits become constricted by the assembly of dynamin into rings around their necks. A concerted conformational change would then close the rings and pinch off the budding coated vesicles. More... »

PAGES

190-192

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/374190a0

DOI

http://dx.doi.org/10.1038/374190a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021608892

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7877694


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clathrin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coated Vesicles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drosophila Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dynamins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "GTP Phosphohydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proline", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spodoptera", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, California, USA", 
          "id": "http://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hinshaw", 
        "givenName": "Jenny E.", 
        "id": "sg:person.01145417075.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145417075.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, California, USA", 
          "id": "http://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schmid", 
        "givenName": "Sandra L.", 
        "id": "sg:person.01235321655.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235321655.35"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/351583a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026966776", 
          "https://doi.org/10.1038/351583a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/351411a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033244420", 
          "https://doi.org/10.1038/351411a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/347256a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028569139", 
          "https://doi.org/10.1038/347256a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/374186a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048309237", 
          "https://doi.org/10.1038/374186a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00318441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015145024", 
          "https://doi.org/10.1007/bf00318441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01737991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031148834", 
          "https://doi.org/10.1007/bf01737991"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1995-03", 
    "datePublishedReg": "1995-03-01", 
    "description": "DYNAMIN, a 100K member of the GTPase superfamily1, is the mammalian homologue of the Drosophila shibire gene product2,3. Mutations in shibire cause a defect in endocytosis leading to accumulation of coated pits and deep imaginations at the plasma membrane of all tissues examined4,5. Similarly, invaginated coated pits accumulate in mammalian cells overexpressing dominant-negative mutants of dynamin, establishing that dynamin is required for the formation of 'constricted' coated pits and for coated vesicle budding6. Whether dynamin functions in the classic GTPase mode as a molecular switch to regulate events leading to coated vesicle budding or instead actively participates as a mechanochemical enzyme driving coated vesicle formation is unclear7. Here we show that dynamin spontaneously self-assembles into rings and stacks of interconnected rings, comparable in dimension to the 'collars' observed at the necks of invaginated coated pits that accumulate at synaptic terminals in shibire flies4. We propose that invaginated coated pits become constricted by the assembly of dynamin into rings around their necks. A concerted conformational change would then close the rings and pinch off the budding coated vesicles.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/374190a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6518", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "374"
      }
    ], 
    "keywords": [
      "coated pits", 
      "coated vesicles", 
      "assembly of dynamin", 
      "coated vesicle formation", 
      "concerted conformational change", 
      "dominant negative mutant", 
      "shibire gene", 
      "dynamin function", 
      "mammalian homologue", 
      "mechanochemical enzyme", 
      "mammalian cells", 
      "dynamin", 
      "plasma membrane", 
      "vesicle formation", 
      "molecular switch", 
      "conformational changes", 
      "deep imaginations", 
      "vesicles", 
      "k members", 
      "synaptic terminals", 
      "shibire", 
      "mutants", 
      "homologues", 
      "endocytosis", 
      "genes", 
      "mutations", 
      "enzyme", 
      "membrane", 
      "assembly", 
      "cells", 
      "accumulation", 
      "pits", 
      "formation", 
      "members", 
      "tissue", 
      "mechanism", 
      "switch", 
      "function", 
      "defects", 
      "collar", 
      "events", 
      "changes", 
      "ring", 
      "terminals", 
      "mode", 
      "stack", 
      "neck", 
      "dimensions", 
      "imagination"
    ], 
    "name": "Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding", 
    "pagination": "190-192", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021608892"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/374190a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7877694"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/374190a0", 
      "https://app.dimensions.ai/details/publication/pub.1021608892"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_293.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/374190a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/374190a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/374190a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/374190a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/374190a0'


 

This table displays all metadata directly associated to this object as RDF triples.

189 TRIPLES      21 PREDICATES      93 URIs      79 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/374190a0 schema:about N09f8daa541d14141aee236abeedf5560
2 N18d261ecb6db4e4a9096fc4d8b209e7f
3 N1d27cd5d35ff4c28b198f4f60cbcd442
4 N222df5b53d8843849eef2e60a69761ee
5 N31276c6b32144b689ab2226ee5e1cf7b
6 N50d65ca6c1b24a26a0a1d05e9439410d
7 N60f460bcbfed4098aef7fdbc3d5e487e
8 Naf94a984ebf741f583b6c860553edb82
9 Nb549faa46c4e49eb9464646a25264c03
10 Nc5fd3720907e40fb8990203fc7cb9ad5
11 Ncb48a0c384c34cc48d5cf9f9df060060
12 Nfa563bf0eda44da783bfaf655b1e2c26
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author Nb22b95ff559746dd9522b2a42b6f9b1e
16 schema:citation sg:pub.10.1007/bf00318441
17 sg:pub.10.1007/bf01737991
18 sg:pub.10.1038/347256a0
19 sg:pub.10.1038/351411a0
20 sg:pub.10.1038/351583a0
21 sg:pub.10.1038/374186a0
22 schema:datePublished 1995-03
23 schema:datePublishedReg 1995-03-01
24 schema:description DYNAMIN, a 100K member of the GTPase superfamily1, is the mammalian homologue of the Drosophila shibire gene product2,3. Mutations in shibire cause a defect in endocytosis leading to accumulation of coated pits and deep imaginations at the plasma membrane of all tissues examined4,5. Similarly, invaginated coated pits accumulate in mammalian cells overexpressing dominant-negative mutants of dynamin, establishing that dynamin is required for the formation of 'constricted' coated pits and for coated vesicle budding6. Whether dynamin functions in the classic GTPase mode as a molecular switch to regulate events leading to coated vesicle budding or instead actively participates as a mechanochemical enzyme driving coated vesicle formation is unclear7. Here we show that dynamin spontaneously self-assembles into rings and stacks of interconnected rings, comparable in dimension to the 'collars' observed at the necks of invaginated coated pits that accumulate at synaptic terminals in shibire flies4. We propose that invaginated coated pits become constricted by the assembly of dynamin into rings around their necks. A concerted conformational change would then close the rings and pinch off the budding coated vesicles.
25 schema:genre article
26 schema:isAccessibleForFree false
27 schema:isPartOf N07d40bb4ada346299e46278088698a4f
28 N1625a78a5e914f65847897307e69d812
29 sg:journal.1018957
30 schema:keywords accumulation
31 assembly
32 assembly of dynamin
33 cells
34 changes
35 coated pits
36 coated vesicle formation
37 coated vesicles
38 collar
39 concerted conformational change
40 conformational changes
41 deep imaginations
42 defects
43 dimensions
44 dominant negative mutant
45 dynamin
46 dynamin function
47 endocytosis
48 enzyme
49 events
50 formation
51 function
52 genes
53 homologues
54 imagination
55 k members
56 mammalian cells
57 mammalian homologue
58 mechanism
59 mechanochemical enzyme
60 members
61 membrane
62 mode
63 molecular switch
64 mutants
65 mutations
66 neck
67 pits
68 plasma membrane
69 ring
70 shibire
71 shibire gene
72 stack
73 switch
74 synaptic terminals
75 terminals
76 tissue
77 vesicle formation
78 vesicles
79 schema:name Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding
80 schema:pagination 190-192
81 schema:productId N54c2e188f57a4dd797873e8c3fea69ed
82 N573ba4666e3c41e5bb6440e202d5acfc
83 Nb5cb9cc80a5a4b1b8e5376f8292bb9db
84 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021608892
85 https://doi.org/10.1038/374190a0
86 schema:sdDatePublished 2022-12-01T06:21
87 schema:sdLicense https://scigraph.springernature.com/explorer/license/
88 schema:sdPublisher N082b201257e34c0ab47bc4fa89ea0fe9
89 schema:url https://doi.org/10.1038/374190a0
90 sgo:license sg:explorer/license/
91 sgo:sdDataset articles
92 rdf:type schema:ScholarlyArticle
93 N07d40bb4ada346299e46278088698a4f schema:issueNumber 6518
94 rdf:type schema:PublicationIssue
95 N082b201257e34c0ab47bc4fa89ea0fe9 schema:name Springer Nature - SN SciGraph project
96 rdf:type schema:Organization
97 N09f8daa541d14141aee236abeedf5560 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Spodoptera
99 rdf:type schema:DefinedTerm
100 N1625a78a5e914f65847897307e69d812 schema:volumeNumber 374
101 rdf:type schema:PublicationVolume
102 N18d261ecb6db4e4a9096fc4d8b209e7f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Recombinant Proteins
104 rdf:type schema:DefinedTerm
105 N1d27cd5d35ff4c28b198f4f60cbcd442 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Proline
107 rdf:type schema:DefinedTerm
108 N222df5b53d8843849eef2e60a69761ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Dynamins
110 rdf:type schema:DefinedTerm
111 N31276c6b32144b689ab2226ee5e1cf7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Neurons
113 rdf:type schema:DefinedTerm
114 N4e0e1dc4d5174da2bd70410e3e5b791f rdf:first sg:person.01235321655.35
115 rdf:rest rdf:nil
116 N50d65ca6c1b24a26a0a1d05e9439410d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Coated Vesicles
118 rdf:type schema:DefinedTerm
119 N54c2e188f57a4dd797873e8c3fea69ed schema:name pubmed_id
120 schema:value 7877694
121 rdf:type schema:PropertyValue
122 N573ba4666e3c41e5bb6440e202d5acfc schema:name dimensions_id
123 schema:value pub.1021608892
124 rdf:type schema:PropertyValue
125 N60f460bcbfed4098aef7fdbc3d5e487e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Clathrin
127 rdf:type schema:DefinedTerm
128 Naf94a984ebf741f583b6c860553edb82 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Animals
130 rdf:type schema:DefinedTerm
131 Nb22b95ff559746dd9522b2a42b6f9b1e rdf:first sg:person.01145417075.54
132 rdf:rest N4e0e1dc4d5174da2bd70410e3e5b791f
133 Nb549faa46c4e49eb9464646a25264c03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name GTP Phosphohydrolases
135 rdf:type schema:DefinedTerm
136 Nb5cb9cc80a5a4b1b8e5376f8292bb9db schema:name doi
137 schema:value 10.1038/374190a0
138 rdf:type schema:PropertyValue
139 Nc5fd3720907e40fb8990203fc7cb9ad5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Drosophila Proteins
141 rdf:type schema:DefinedTerm
142 Ncb48a0c384c34cc48d5cf9f9df060060 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Cell Line
144 rdf:type schema:DefinedTerm
145 Nfa563bf0eda44da783bfaf655b1e2c26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Humans
147 rdf:type schema:DefinedTerm
148 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
149 schema:name Biological Sciences
150 rdf:type schema:DefinedTerm
151 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
152 schema:name Biochemistry and Cell Biology
153 rdf:type schema:DefinedTerm
154 sg:journal.1018957 schema:issn 0028-0836
155 1476-4687
156 schema:name Nature
157 schema:publisher Springer Nature
158 rdf:type schema:Periodical
159 sg:person.01145417075.54 schema:affiliation grid-institutes:grid.214007.0
160 schema:familyName Hinshaw
161 schema:givenName Jenny E.
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145417075.54
163 rdf:type schema:Person
164 sg:person.01235321655.35 schema:affiliation grid-institutes:grid.214007.0
165 schema:familyName Schmid
166 schema:givenName Sandra L.
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235321655.35
168 rdf:type schema:Person
169 sg:pub.10.1007/bf00318441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015145024
170 https://doi.org/10.1007/bf00318441
171 rdf:type schema:CreativeWork
172 sg:pub.10.1007/bf01737991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031148834
173 https://doi.org/10.1007/bf01737991
174 rdf:type schema:CreativeWork
175 sg:pub.10.1038/347256a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028569139
176 https://doi.org/10.1038/347256a0
177 rdf:type schema:CreativeWork
178 sg:pub.10.1038/351411a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033244420
179 https://doi.org/10.1038/351411a0
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/351583a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026966776
182 https://doi.org/10.1038/351583a0
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/374186a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048309237
185 https://doi.org/10.1038/374186a0
186 rdf:type schema:CreativeWork
187 grid-institutes:grid.214007.0 schema:alternateName Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, California, USA
188 schema:name Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, California, USA
189 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...