A cell initiating human acute myeloid leukaemia after transplantation into SCID mice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-02

AUTHORS

T Lapidot, C Sirard, J Vormoor, B Murdoch, T Hoang, J Caceres-Cortes, M Minden, B Paterson, M A Caligiuri, J E Dick

ABSTRACT

Most human acute myeloid leukaemia (AML) cells have limited proliferative capacity, suggesting that the leukaemic clone may be maintained by a rare population of stem cells. This putative leukaemic stem cell has not been characterized because the available in vitro assays can only detect progenitors with limited proliferative and replating potential. We have now identified an AML-initiating cell by transplantation into severe combined immune-deficient (SCID) mice. These cells homed to the bone marrow and proliferated extensively in response to in vivo cytokine treatment, resulting in a pattern of dissemination and leukaemic cell morphology similar to that seen in the original patients. Limiting dilution analysis showed that the frequency of these leukaemia-initiating cells in the peripheral blood of AML patients was one engraftment unit in 250,000 cells. We fractionated AML cells on the basis of cell-surface-marker expression and found that the leukaemia-initiating cells that could engraft SCID mice to produce large numbers of colony-forming progenitors were CD34+ CD38-; however, the CD34+ CD38+ and CD34- fractions contained no cells with these properties. This in vivo model replicates many aspects of human AML and defines a new leukaemia-initiating cell which is less mature than colony-forming cells. More... »

PAGES

645-648

Journal

TITLE

Nature

ISSUE

6464

VOLUME

367

Author Affiliations

Related Patents

  • Antibodies To Notch Receptors
  • Method For Flow Cytometry Linked With Elemental Analysis
  • Therapeutic Combination And Methods Of Treatment With A Dll4 Antagonist And An Anti-Hypertensive Agent
  • Antibodies That Bind Specifically To Phosphorylated Β-Catenin
  • Compositions And Methods For Characterizing, Regulating, Diagnosing And Treating Cancer
  • Polynucleotides Encoding Vegf/Dll4 Binding Agents
  • Combination Therapy For The Treatment Of Cancer
  • Human Cancer Stem Cells
  • Culturing Embryonic Stem Cells, Embryonic Stem-Like Cells, Or Induced Pluripotent Stem Cells With A Muc1 Or Muc1* Ligand
  • Notch1 Binding Agents And Methods Of Use Thereof
  • Compositions And Methods For Inhibiting Cancer Stem Cells
  • Human Cancer Stem Cells
  • Ddr1-Binding Agents And Methods Of Use Thereof
  • Notch1 Receptor Binding Agents And Methods Of Use Thereof
  • Methods Of Treatment With Dll4 Antagonists And An Anti-Hypertensive Agent
  • Therapeutic Combination And Methods Of Treatment With A Dll4 Antagonist And An Anti-Hypertensive Agent
  • Immunotherapeutic Method Involving Cd123 (Il-3rα) Antibodies And Immunostimulating Complex
  • Therapeutic And Diagnostic Applications Of Genes Differentially Expressed In Lympho-Hematopoietic Stem Cells
  • Method For Treating Cancer Using A Notch4 Ligand Antagonist
  • Isolation And Use Of Solid Tumor Stem Cells
  • Modulators Of Development Of Adipocyte And Cancer Cells
  • Bisacodyl And Analogues As Drugs For Treating Cancer
  • Antibodies To Notch Receptors
  • Method And Apparatus For Flow Cytometry Linked With Elemental Analysis
  • Methods To Modulate Acute Myeloid Leukemia Stem/Progenitor Cell Expansion And/Or Differentiation
  • Compositions And Methods For Diagnosing And Treating Cancer
  • Determining The Capability Of A Test Compound To Affect Solid Tumor Stem Cells
  • Vegf Binding Antibodies
  • A Method For Identifying, Expanding, And Removing Adult Stem Cells And Cancer Stem Cells
  • Modified Immunoglobulin Molecules That Specifically Bind Human Vegf And Dll4
  • Immunotherapeutic Method Involving Cd123 (Il-3rα) Antibodies And Immunostimulating Complex
  • Compositions And Methods For Diagnosing And Treating Cancer
  • Method Of Treating Cancer Using Antibodies To A Non-Ligand Binding Region Of Notch2
  • Method For Identifying, Expanding, And Removing Adult Stem Cells And Cancer Stem Cells
  • Antibodies To A Non-Ligand Binding Region Of At Least Two Notch Receptors
  • Compositions And Methods For Diagnosing And Treating Cancer
  • Trispecific Therapeutics Against Acute Myeloid Leukaemia
  • Trispecific Therapeutics Against Acute Myeloid Leukaemia
  • Antibodies That Bind The Glutamate Ligand Binding Region Of Notch1
  • Use Of Fenretinide Or Bioactive Derivatives Thereof And Pharmaceutical Compositions Comprising The Same
  • Methods For Treating Melanoma
  • Sirna Compositions And Methods For Inhibiting Gene Expression In Tumor Initiating Cells Of Breast Cancer
  • Compositions And Methods For Treating And Diagnosing Cancer
  • Method For Treating Cancer Using An Antibody That Inhibits Notch4 Signaling
  • Methods Of Using Antibodies That Bind The Glutamate Ligand Binding Region Of Notch1
  • In Vivo Method For Treating, Inhibiting, And/Or Prophylaxis Of Cancer, Such As Pancreatic Cancer
  • Culture Of Leukemia Initiating Cells
  • Notch1 Receptor Antibodies And Methods Of Treatment
  • Polynucleotides Encoding Notch Receptor Antibodies
  • Cancer Stem Cell Isolation
  • Cancer Stem Cell-Specific Molecule
  • Identification And Isolation Of Transitional Cell Carcinoma Stem Cells
  • Identification And Isolation Of Acute Myeloid Leukemia Stem Cells
  • Isolation And Use Of Solid Tumor Stem Cells
  • Methods And Monitoring Of Treatment With A Dll4 Antagonist
  • Antibodies To The Notch1 Receptor
  • Compositions And Methods For Treating And Diagnosing Cancer
  • Determining The Capability Of A Test Compound To Affect Solid Tumor Stem Cells
  • Anti-Human Dll4 Antibodies And Compositions
  • Monoclonal Antibody That Binds Human Notch2 And Notch3
  • Method For Identifying, Expanding And Removing Adult Stem Cells And Cancer Stem Cells
  • Siglec-9 Binding Agents
  • Methods For Treating Melanoma
  • Compositions And Methods For Diagnosing And Treating Cancer
  • Therapeutic Composition For Treatment Of Glioblastoma
  • Proliferation Of Muc1 Expressing Cells
  • Compositions And Methods For Treating And Diagnosing Pancreatic Cancer
  • Therapeutic Composition For Treatment Of Glioblastoma
  • Compound For Treating Cancer And Diabetes
  • Polynucleotides Encoding Notch1 Receptor Antibodies
  • Notch1 Binding Agents And Methods Of Use Thereof
  • Methods For Stimulating Or Enhancing Proliferation Of Non-Tumorous Cells Expressing Muc1 Receptors
  • Methods Of Treatment By Administering Antibodies To Notch Receptors
  • Combination Therapy For The Treatment Of Cancer
  • Compositions And Methods For Treating And Diagnosing Pancreatic Cancer
  • Method Of Treating Cancer By Administering A Monoclonal Antibody That Binds Human Notch2 And Notch3
  • Notch1 Receptor Antibodies And Methods Of Treatment
  • Leukemia Stem Cell Markers
  • Cancer Stem Cells And Uses Thereof
  • Antibodies That Bind The Glutamate Ligand Binding Region Of Notch3
  • Method Of Detecting Phosphorylation State Of Beta-Catenin
  • Vegf/Dll4 Binding Agents And Uses Thereof
  • Inos-Inhibitory Compositions And Their Use As Breast Cancer Therapeutics
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/367645a0

    DOI

    http://dx.doi.org/10.1038/367645a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028822583

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7509044


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Oncology and Carcinogenesis", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "ADP-ribosyl Cyclase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "ADP-ribosyl Cyclase 1", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acute Disease", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antigens, CD", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antigens, CD34", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antigens, Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bone Marrow", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Division", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Movement", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Granulocyte-Macrophage Colony-Stimulating Factor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hematopoietic Cell Growth Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunophenotyping", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Interleukin-3", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Leukemia, Myeloid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Membrane Glycoproteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, SCID", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplastic Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Fusion Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cell Factor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transplantation, Heterologous", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Hospital for Sick Children", 
              "id": "https://www.grid.ac/institutes/grid.42327.30", 
              "name": [
                "Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lapidot", 
            "givenName": "T", 
            "id": "sg:person.0760144763.90", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760144763.90"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Sirard", 
            "givenName": "C", 
            "id": "sg:person.0616024454.33", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616024454.33"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Vormoor", 
            "givenName": "J", 
            "id": "sg:person.01270712731.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270712731.10"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Murdoch", 
            "givenName": "B", 
            "id": "sg:person.01114132705.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114132705.25"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Hoang", 
            "givenName": "T", 
            "id": "sg:person.0730341606.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730341606.34"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Caceres-Cortes", 
            "givenName": "J", 
            "id": "sg:person.01046401060.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046401060.01"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Minden", 
            "givenName": "M", 
            "id": "sg:person.01143512370.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143512370.79"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Paterson", 
            "givenName": "B", 
            "id": "sg:person.01162730054.97", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162730054.97"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Caligiuri", 
            "givenName": "M A", 
            "id": "sg:person.01167105107.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167105107.83"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Dick", 
            "givenName": "J E", 
            "id": "sg:person.016114074342.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016114074342.05"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0092-8674(91)90643-d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001082055"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1600-065x.1991.tb00614.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002672267"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci111797", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013745063"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.6.9.3156", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014685349"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcp.1041130417", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028224762"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejm198607033150103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028783950"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/bjc.1963.78", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031401152", 
              "https://doi.org/10.1038/bjc.1963.78"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/bjc.1963.78", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031401152", 
              "https://doi.org/10.1038/bjc.1963.78"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01732875", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039646994", 
              "https://doi.org/10.1007/bf01732875"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01732875", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039646994", 
              "https://doi.org/10.1007/bf01732875"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1372131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062478159"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2595371", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062545583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2904703", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062572580"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076052605", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076986884", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077005501", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077312306", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077622031", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079807579", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081841536", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082800589", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1994-02", 
        "datePublishedReg": "1994-02-01", 
        "description": "Most human acute myeloid leukaemia (AML) cells have limited proliferative capacity, suggesting that the leukaemic clone may be maintained by a rare population of stem cells. This putative leukaemic stem cell has not been characterized because the available in vitro assays can only detect progenitors with limited proliferative and replating potential. We have now identified an AML-initiating cell by transplantation into severe combined immune-deficient (SCID) mice. These cells homed to the bone marrow and proliferated extensively in response to in vivo cytokine treatment, resulting in a pattern of dissemination and leukaemic cell morphology similar to that seen in the original patients. Limiting dilution analysis showed that the frequency of these leukaemia-initiating cells in the peripheral blood of AML patients was one engraftment unit in 250,000 cells. We fractionated AML cells on the basis of cell-surface-marker expression and found that the leukaemia-initiating cells that could engraft SCID mice to produce large numbers of colony-forming progenitors were CD34+ CD38-; however, the CD34+ CD38+ and CD34- fractions contained no cells with these properties. This in vivo model replicates many aspects of human AML and defines a new leukaemia-initiating cell which is less mature than colony-forming cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/367645a0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6464", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "367"
          }
        ], 
        "name": "A cell initiating human acute myeloid leukaemia after transplantation into SCID mice", 
        "pagination": "645-648", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "894e1013b7fb4e372aad3f8ffeb664fb8e9f43ac80b5a28ba8e0c6f433431342"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "7509044"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/367645a0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1028822583"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/367645a0", 
          "https://app.dimensions.ai/details/publication/pub.1028822583"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T13:55", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000425.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/367645a0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/367645a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/367645a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/367645a0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/367645a0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    266 TRIPLES      21 PREDICATES      71 URIs      44 LITERALS      32 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/367645a0 schema:about N26bbb610aa894febacceff04971da262
    2 N2f698280e5c34e07a8990d9930d7bb9b
    3 N35fc9233c64c439c8889a01663f6ee19
    4 N41afd3698c21455dbb21a678011c836a
    5 N47f75f35fad84051a071256b5afb782d
    6 N5412caba3fb84a918e75f32bc5287b39
    7 N6470550b2de84221b1ea68aa2dc477e4
    8 N69adf2155578408b948fccaf84104af5
    9 N8534ab46f53a4f71a608b9f5b9ba077d
    10 N86e699f41f374962af195234a2467ca4
    11 N8851f8b3893d4fe09386b120f7a22636
    12 Na479a633b950477ebe7eb9d63cfe4e47
    13 Nbffd6de4359d456385cd3c0994cf4a6c
    14 Nc4b0004886b14040834439b92f683695
    15 Nd08b1810555d44aa9b4fc6b5b7469680
    16 Nd2a6153f6b4d46358bab13d85f03140b
    17 Nd942a8e32bd845778e97ef6e21830dbf
    18 Ne7b73e08293741d0ba44ecd515a881dc
    19 Neaf9112add2e4825aec94688632ce162
    20 Nf2f52d6401cb462ea786cd2ccdaa0d88
    21 Nf4e0afb03c29486a8410ff41eb3fe9e7
    22 Nf72683c30d92487e80053cbd9f3184d6
    23 Nfe33ce616aea4bbe8faa9b0fe4453756
    24 anzsrc-for:11
    25 anzsrc-for:1112
    26 schema:author N2f24cab07ac14c0fba8ae9eb6983f698
    27 schema:citation sg:pub.10.1007/bf01732875
    28 sg:pub.10.1038/bjc.1963.78
    29 https://app.dimensions.ai/details/publication/pub.1076052605
    30 https://app.dimensions.ai/details/publication/pub.1076986884
    31 https://app.dimensions.ai/details/publication/pub.1077005501
    32 https://app.dimensions.ai/details/publication/pub.1077312306
    33 https://app.dimensions.ai/details/publication/pub.1077622031
    34 https://app.dimensions.ai/details/publication/pub.1079807579
    35 https://app.dimensions.ai/details/publication/pub.1081841536
    36 https://app.dimensions.ai/details/publication/pub.1082800589
    37 https://doi.org/10.1002/jcp.1041130417
    38 https://doi.org/10.1016/0092-8674(91)90643-d
    39 https://doi.org/10.1056/nejm198607033150103
    40 https://doi.org/10.1111/j.1600-065x.1991.tb00614.x
    41 https://doi.org/10.1126/science.1372131
    42 https://doi.org/10.1126/science.2595371
    43 https://doi.org/10.1126/science.2904703
    44 https://doi.org/10.1128/mcb.6.9.3156
    45 https://doi.org/10.1172/jci111797
    46 schema:datePublished 1994-02
    47 schema:datePublishedReg 1994-02-01
    48 schema:description Most human acute myeloid leukaemia (AML) cells have limited proliferative capacity, suggesting that the leukaemic clone may be maintained by a rare population of stem cells. This putative leukaemic stem cell has not been characterized because the available in vitro assays can only detect progenitors with limited proliferative and replating potential. We have now identified an AML-initiating cell by transplantation into severe combined immune-deficient (SCID) mice. These cells homed to the bone marrow and proliferated extensively in response to in vivo cytokine treatment, resulting in a pattern of dissemination and leukaemic cell morphology similar to that seen in the original patients. Limiting dilution analysis showed that the frequency of these leukaemia-initiating cells in the peripheral blood of AML patients was one engraftment unit in 250,000 cells. We fractionated AML cells on the basis of cell-surface-marker expression and found that the leukaemia-initiating cells that could engraft SCID mice to produce large numbers of colony-forming progenitors were CD34+ CD38-; however, the CD34+ CD38+ and CD34- fractions contained no cells with these properties. This in vivo model replicates many aspects of human AML and defines a new leukaemia-initiating cell which is less mature than colony-forming cells.
    49 schema:genre research_article
    50 schema:inLanguage en
    51 schema:isAccessibleForFree false
    52 schema:isPartOf N4b250ba328f24218987d210d74dfabcd
    53 N74681eda5b30430da362b7d04ad0f5f9
    54 sg:journal.1018957
    55 schema:name A cell initiating human acute myeloid leukaemia after transplantation into SCID mice
    56 schema:pagination 645-648
    57 schema:productId N47f945f18dd2459087a7a276169a6a28
    58 N707b06ba19d147bf85588809c6f52d91
    59 N7fb90f957a434ff3ac78ef2be2f49791
    60 Nb8a0141cc25d47a5a94e03627d30a71f
    61 Ne2ad8c8a050541da9a71f18a5aac1c7a
    62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028822583
    63 https://doi.org/10.1038/367645a0
    64 schema:sdDatePublished 2019-04-10T13:55
    65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    66 schema:sdPublisher N9a7f568aa0c344c7a403bc00f974aa67
    67 schema:url http://www.nature.com/articles/367645a0
    68 sgo:license sg:explorer/license/
    69 sgo:sdDataset articles
    70 rdf:type schema:ScholarlyArticle
    71 N04b769ccea484be7927f34a7dd90e30c rdf:first sg:person.01270712731.10
    72 rdf:rest Ndfe0f7a790c34af4a79856cda1378e5b
    73 N26bbb610aa894febacceff04971da262 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Recombinant Fusion Proteins
    75 rdf:type schema:DefinedTerm
    76 N2f24cab07ac14c0fba8ae9eb6983f698 rdf:first sg:person.0760144763.90
    77 rdf:rest N4c91566201b848a4b032ffcff68b9c7d
    78 N2f698280e5c34e07a8990d9930d7bb9b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Antigens, CD
    80 rdf:type schema:DefinedTerm
    81 N35fc9233c64c439c8889a01663f6ee19 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Granulocyte-Macrophage Colony-Stimulating Factor
    83 rdf:type schema:DefinedTerm
    84 N41afd3698c21455dbb21a678011c836a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Transplantation, Heterologous
    86 rdf:type schema:DefinedTerm
    87 N47f75f35fad84051a071256b5afb782d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Antigens, CD34
    89 rdf:type schema:DefinedTerm
    90 N47f945f18dd2459087a7a276169a6a28 schema:name doi
    91 schema:value 10.1038/367645a0
    92 rdf:type schema:PropertyValue
    93 N4b250ba328f24218987d210d74dfabcd schema:volumeNumber 367
    94 rdf:type schema:PublicationVolume
    95 N4c91566201b848a4b032ffcff68b9c7d rdf:first sg:person.0616024454.33
    96 rdf:rest N04b769ccea484be7927f34a7dd90e30c
    97 N5412caba3fb84a918e75f32bc5287b39 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Animals
    99 rdf:type schema:DefinedTerm
    100 N622455bcc88f43df81de63613d72b8f6 rdf:first sg:person.016114074342.05
    101 rdf:rest rdf:nil
    102 N62d78cc9646c4ab09578872599c50144 rdf:first sg:person.0730341606.34
    103 rdf:rest N963405fefaf0420fb768047f67e19028
    104 N6470550b2de84221b1ea68aa2dc477e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Cell Division
    106 rdf:type schema:DefinedTerm
    107 N69adf2155578408b948fccaf84104af5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Acute Disease
    109 rdf:type schema:DefinedTerm
    110 N707b06ba19d147bf85588809c6f52d91 schema:name pubmed_id
    111 schema:value 7509044
    112 rdf:type schema:PropertyValue
    113 N74681eda5b30430da362b7d04ad0f5f9 schema:issueNumber 6464
    114 rdf:type schema:PublicationIssue
    115 N7fb90f957a434ff3ac78ef2be2f49791 schema:name nlm_unique_id
    116 schema:value 0410462
    117 rdf:type schema:PropertyValue
    118 N8534ab46f53a4f71a608b9f5b9ba077d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Bone Marrow
    120 rdf:type schema:DefinedTerm
    121 N86e699f41f374962af195234a2467ca4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Antigens, Differentiation
    123 rdf:type schema:DefinedTerm
    124 N8851f8b3893d4fe09386b120f7a22636 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Hematopoietic Cell Growth Factors
    126 rdf:type schema:DefinedTerm
    127 N963405fefaf0420fb768047f67e19028 rdf:first sg:person.01046401060.01
    128 rdf:rest N9915694dce9546d89af1527617a8b6ae
    129 N9915694dce9546d89af1527617a8b6ae rdf:first sg:person.01143512370.79
    130 rdf:rest Nb6e53a3a8e3146b588166bcc65ed51c8
    131 N9a7f568aa0c344c7a403bc00f974aa67 schema:name Springer Nature - SN SciGraph project
    132 rdf:type schema:Organization
    133 Na479a633b950477ebe7eb9d63cfe4e47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Interleukin-3
    135 rdf:type schema:DefinedTerm
    136 Nb6e53a3a8e3146b588166bcc65ed51c8 rdf:first sg:person.01162730054.97
    137 rdf:rest Nd590e9aaa32645e9aae5abfff5ec44a5
    138 Nb8a0141cc25d47a5a94e03627d30a71f schema:name readcube_id
    139 schema:value 894e1013b7fb4e372aad3f8ffeb664fb8e9f43ac80b5a28ba8e0c6f433431342
    140 rdf:type schema:PropertyValue
    141 Nbffd6de4359d456385cd3c0994cf4a6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Leukemia, Myeloid
    143 rdf:type schema:DefinedTerm
    144 Nc4b0004886b14040834439b92f683695 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    145 schema:name Humans
    146 rdf:type schema:DefinedTerm
    147 Nd08b1810555d44aa9b4fc6b5b7469680 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Immunophenotyping
    149 rdf:type schema:DefinedTerm
    150 Nd2a6153f6b4d46358bab13d85f03140b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name ADP-ribosyl Cyclase 1
    152 rdf:type schema:DefinedTerm
    153 Nd590e9aaa32645e9aae5abfff5ec44a5 rdf:first sg:person.01167105107.83
    154 rdf:rest N622455bcc88f43df81de63613d72b8f6
    155 Nd942a8e32bd845778e97ef6e21830dbf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Mice
    157 rdf:type schema:DefinedTerm
    158 Ndfe0f7a790c34af4a79856cda1378e5b rdf:first sg:person.01114132705.25
    159 rdf:rest N62d78cc9646c4ab09578872599c50144
    160 Ne2ad8c8a050541da9a71f18a5aac1c7a schema:name dimensions_id
    161 schema:value pub.1028822583
    162 rdf:type schema:PropertyValue
    163 Ne7b73e08293741d0ba44ecd515a881dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Stem Cell Factor
    165 rdf:type schema:DefinedTerm
    166 Neaf9112add2e4825aec94688632ce162 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name Membrane Glycoproteins
    168 rdf:type schema:DefinedTerm
    169 Nf2f52d6401cb462ea786cd2ccdaa0d88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    170 schema:name Neoplastic Stem Cells
    171 rdf:type schema:DefinedTerm
    172 Nf4e0afb03c29486a8410ff41eb3fe9e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    173 schema:name Cell Movement
    174 rdf:type schema:DefinedTerm
    175 Nf72683c30d92487e80053cbd9f3184d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    176 schema:name ADP-ribosyl Cyclase
    177 rdf:type schema:DefinedTerm
    178 Nfe33ce616aea4bbe8faa9b0fe4453756 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name Mice, SCID
    180 rdf:type schema:DefinedTerm
    181 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    182 schema:name Medical and Health Sciences
    183 rdf:type schema:DefinedTerm
    184 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
    185 schema:name Oncology and Carcinogenesis
    186 rdf:type schema:DefinedTerm
    187 sg:journal.1018957 schema:issn 0090-0028
    188 1476-4687
    189 schema:name Nature
    190 rdf:type schema:Periodical
    191 sg:person.01046401060.01 schema:familyName Caceres-Cortes
    192 schema:givenName J
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046401060.01
    194 rdf:type schema:Person
    195 sg:person.01114132705.25 schema:familyName Murdoch
    196 schema:givenName B
    197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114132705.25
    198 rdf:type schema:Person
    199 sg:person.01143512370.79 schema:familyName Minden
    200 schema:givenName M
    201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143512370.79
    202 rdf:type schema:Person
    203 sg:person.01162730054.97 schema:familyName Paterson
    204 schema:givenName B
    205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01162730054.97
    206 rdf:type schema:Person
    207 sg:person.01167105107.83 schema:familyName Caligiuri
    208 schema:givenName M A
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167105107.83
    210 rdf:type schema:Person
    211 sg:person.01270712731.10 schema:familyName Vormoor
    212 schema:givenName J
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270712731.10
    214 rdf:type schema:Person
    215 sg:person.016114074342.05 schema:familyName Dick
    216 schema:givenName J E
    217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016114074342.05
    218 rdf:type schema:Person
    219 sg:person.0616024454.33 schema:familyName Sirard
    220 schema:givenName C
    221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616024454.33
    222 rdf:type schema:Person
    223 sg:person.0730341606.34 schema:familyName Hoang
    224 schema:givenName T
    225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730341606.34
    226 rdf:type schema:Person
    227 sg:person.0760144763.90 schema:affiliation https://www.grid.ac/institutes/grid.42327.30
    228 schema:familyName Lapidot
    229 schema:givenName T
    230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760144763.90
    231 rdf:type schema:Person
    232 sg:pub.10.1007/bf01732875 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039646994
    233 https://doi.org/10.1007/bf01732875
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1038/bjc.1963.78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031401152
    236 https://doi.org/10.1038/bjc.1963.78
    237 rdf:type schema:CreativeWork
    238 https://app.dimensions.ai/details/publication/pub.1076052605 schema:CreativeWork
    239 https://app.dimensions.ai/details/publication/pub.1076986884 schema:CreativeWork
    240 https://app.dimensions.ai/details/publication/pub.1077005501 schema:CreativeWork
    241 https://app.dimensions.ai/details/publication/pub.1077312306 schema:CreativeWork
    242 https://app.dimensions.ai/details/publication/pub.1077622031 schema:CreativeWork
    243 https://app.dimensions.ai/details/publication/pub.1079807579 schema:CreativeWork
    244 https://app.dimensions.ai/details/publication/pub.1081841536 schema:CreativeWork
    245 https://app.dimensions.ai/details/publication/pub.1082800589 schema:CreativeWork
    246 https://doi.org/10.1002/jcp.1041130417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028224762
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1016/0092-8674(91)90643-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1001082055
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1056/nejm198607033150103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028783950
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1111/j.1600-065x.1991.tb00614.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002672267
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1126/science.1372131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062478159
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1126/science.2595371 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062545583
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1126/science.2904703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062572580
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1128/mcb.6.9.3156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014685349
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1172/jci111797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013745063
    263 rdf:type schema:CreativeWork
    264 https://www.grid.ac/institutes/grid.42327.30 schema:alternateName Hospital for Sick Children
    265 schema:name Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
    266 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...