Functional ecdysone receptor is the product of EcR and Ultraspiracle genes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-12

AUTHORS

Tso-Pang Yao, Barry Marc Forman, Zeyu Jiang, Lucy Cherbas, J.-Don Chen, Michael McKeown, Peter Cherbas, Ronald M. Evans

ABSTRACT

ALTHOUGH the biological activity of the insect moulting hormone ecdysone, is manifested through a hormonally regulated transcriptional cascade associated with chromosomal puffing1–3, a direct association of the receptor with the puff has yet to be established. The cloned ecdysone receptor4 (EcR) is by itself incapable of high-affinity DNA binding or transcriptional activation. Rather, these activities are dependent on heterodimer formation with Ultraspiracle5 (USP) the insect homologue of vertebrate retinoid X receptor6. Here we report that native EcR and USP are co-localized on ecdysone-responsive loci of polytene chromosomes. Moreover, we show that natural ecdysones selectively promote physical association between EcR and USP, and conversely, that high-affinity hormone binding requires both EcR and USP. Replacement of USP with retinoid X receptor produces heterodimers with distinct pharmacological and functional properties. These results redefine the ecdysone receptor as a dynamic complex whose activity may be altered by combinatorial interactions among subunits and ligand. More... »

PAGES

476-479

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

6454

VOLUME

366

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/366476a0

    DOI

    http://dx.doi.org/10.1038/366476a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010990226

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8247157


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