Multi-ion pore behaviour in the CFTR chloride channel View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-11

AUTHORS

J A Tabcharani, J M Rommens, Y X Hou, X B Chang, L C Tsui, J R Riordan, J W Hanrahan

ABSTRACT

Cystic fibrosis transmembrane conductance regulator (CFTR) is a non-rectifying, low-conductance channel regulated by ATP and phosphorylation, which mediates apical chloride conductance in secretory epithelia and malfunctions in cystic fibrosis (CF). Mutations at Lys 335 and Arg 347 in the sixth predicted transmembrane helix of CFTR alter its halide selectivity in whole-cell studies and its single channel conductance, but the physical basis of these alterations is unknown and permeation in CFTR is poorly understood. Here we present evidence that wild-type CFTR can contain more than one anion simultaneously. The conductance of CFTR passes through a minimum when channels are bathed in mixtures of two permeant anions. This anomalous mole fraction effect can be abolished by replacing Arg 347 with an aspartate and can be toggled on or off by varying the pH after the same residue is replaced with a histidine. Thus the CFTR channel should provide a convenient model in which to study multi-ion pore behaviour and conduction. The loss of multiple occupancy may explain how naturally occurring CF mutations at this site cause disease. More... »

PAGES

79-82

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/366079a0

DOI

http://dx.doi.org/10.1038/366079a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029316472

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7694154


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arginine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CHO Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chloride Channels", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chlorides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetinae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cystic Fibrosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cystic Fibrosis Transmembrane Conductance Regulator", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ion Channels", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Potentials", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Site-Directed", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thiocyanates", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "McGill University", 
          "id": "https://www.grid.ac/institutes/grid.14709.3b", 
          "name": [
            "Department of Physiology, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tabcharani", 
        "givenName": "J A", 
        "id": "sg:person.0617234553.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617234553.55"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Rommens", 
        "givenName": "J M", 
        "id": "sg:person.01355172356.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355172356.46"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Hou", 
        "givenName": "Y X", 
        "id": "sg:person.01361307674.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361307674.99"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Chang", 
        "givenName": "X B", 
        "id": "sg:person.01310536143.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310536143.87"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Tsui", 
        "givenName": "L C", 
        "id": "sg:person.01243057047.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243057047.05"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Riordan", 
        "givenName": "J R", 
        "id": "sg:person.01110637425.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110637425.01"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Hanrahan", 
        "givenName": "J W", 
        "id": "sg:person.0732464671.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732464671.04"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0092-8674(91)90498-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001366314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.15.7351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002118380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1113/jphysiol.1984.sp015352", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004807693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(90)81257-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006933539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90398-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007064578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/309453a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015077827", 
          "https://doi.org/10.1038/309453a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021797759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021797759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/362160a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026041580", 
          "https://doi.org/10.1038/362160a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01871272", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030522845", 
          "https://doi.org/10.1007/bf01871272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01871272", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030522845", 
          "https://doi.org/10.1007/bf01871272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.61.6.687", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032026837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/347358a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035172743", 
          "https://doi.org/10.1038/347358a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/humu.1380010409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037293842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/humu.1380010409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037293842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(91)90072-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040830484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/352628a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044633103", 
          "https://doi.org/10.1038/352628a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90155-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047418822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/2.1.51", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059644060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1712984", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062502499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075993156", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpcell.1989.257.2.c240", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078898779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082464554", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-11", 
    "datePublishedReg": "1993-11-01", 
    "description": "Cystic fibrosis transmembrane conductance regulator (CFTR) is a non-rectifying, low-conductance channel regulated by ATP and phosphorylation, which mediates apical chloride conductance in secretory epithelia and malfunctions in cystic fibrosis (CF). Mutations at Lys 335 and Arg 347 in the sixth predicted transmembrane helix of CFTR alter its halide selectivity in whole-cell studies and its single channel conductance, but the physical basis of these alterations is unknown and permeation in CFTR is poorly understood. Here we present evidence that wild-type CFTR can contain more than one anion simultaneously. The conductance of CFTR passes through a minimum when channels are bathed in mixtures of two permeant anions. This anomalous mole fraction effect can be abolished by replacing Arg 347 with an aspartate and can be toggled on or off by varying the pH after the same residue is replaced with a histidine. Thus the CFTR channel should provide a convenient model in which to study multi-ion pore behaviour and conduction. The loss of multiple occupancy may explain how naturally occurring CF mutations at this site cause disease.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/366079a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6450", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "366"
      }
    ], 
    "name": "Multi-ion pore behaviour in the CFTR chloride channel", 
    "pagination": "79-82", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8ba8d022320efb1c4490f876427b975122ee6a62507954ada25e58d53a4b6292"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7694154"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/366079a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029316472"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/366079a0", 
      "https://app.dimensions.ai/details/publication/pub.1029316472"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000469.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/366079a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/366079a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/366079a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/366079a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/366079a0'


 

This table displays all metadata directly associated to this object as RDF triples.

232 TRIPLES      21 PREDICATES      65 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/366079a0 schema:about N17cacd268a95417380e303f8d6c7eb97
2 N1cca4ddfe9c24f59a1cb869884818074
3 N3c17a6ce805f44189a3e8ea3820ceabf
4 N513510ed038c4223b0d18c81d54c8810
5 N59928d3f9efe402db608480ded788b93
6 N59a540ff4cb7495695e60681906c555f
7 N5cffb73bde284ab88c75599701c012a7
8 N634e27e375b742aea0ba2744bb6f6132
9 N6a3aa1dc3d1b4a399af924ba376440cf
10 Naebb0412ce1e46f2b439c2d370483e61
11 Nb15ed794defc42178149f54ed6956ec5
12 Nb7d462416b6243829f2f8c5d24ab3faa
13 Nc0cc970b28ef4bdfaa911632c01838a2
14 Ndc4137379470443fb2eb17795ec443aa
15 Ne583d11e5290469caa4ccd65600d467e
16 Nf9062e766f9a43ddab51e194473a32ee
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author Nd9a0f38f719541749f52ecb4437eb435
20 schema:citation sg:pub.10.1007/bf01871272
21 sg:pub.10.1038/309453a0
22 sg:pub.10.1038/347358a0
23 sg:pub.10.1038/352628a0
24 sg:pub.10.1038/362160a0
25 https://app.dimensions.ai/details/publication/pub.1075993156
26 https://app.dimensions.ai/details/publication/pub.1082464554
27 https://doi.org/10.1002/humu.1380010409
28 https://doi.org/10.1016/0014-5793(90)81257-o
29 https://doi.org/10.1016/0092-8674(90)90398-x
30 https://doi.org/10.1016/0092-8674(91)90072-7
31 https://doi.org/10.1016/0092-8674(91)90498-n
32 https://doi.org/10.1016/0092-8674(92)90155-6
33 https://doi.org/10.1016/0378-1119(89)90358-2
34 https://doi.org/10.1085/jgp.61.6.687
35 https://doi.org/10.1093/hmg/2.1.51
36 https://doi.org/10.1093/nar/16.15.7351
37 https://doi.org/10.1113/jphysiol.1984.sp015352
38 https://doi.org/10.1126/science.1712984
39 https://doi.org/10.1152/ajpcell.1989.257.2.c240
40 schema:datePublished 1993-11
41 schema:datePublishedReg 1993-11-01
42 schema:description Cystic fibrosis transmembrane conductance regulator (CFTR) is a non-rectifying, low-conductance channel regulated by ATP and phosphorylation, which mediates apical chloride conductance in secretory epithelia and malfunctions in cystic fibrosis (CF). Mutations at Lys 335 and Arg 347 in the sixth predicted transmembrane helix of CFTR alter its halide selectivity in whole-cell studies and its single channel conductance, but the physical basis of these alterations is unknown and permeation in CFTR is poorly understood. Here we present evidence that wild-type CFTR can contain more than one anion simultaneously. The conductance of CFTR passes through a minimum when channels are bathed in mixtures of two permeant anions. This anomalous mole fraction effect can be abolished by replacing Arg 347 with an aspartate and can be toggled on or off by varying the pH after the same residue is replaced with a histidine. Thus the CFTR channel should provide a convenient model in which to study multi-ion pore behaviour and conduction. The loss of multiple occupancy may explain how naturally occurring CF mutations at this site cause disease.
43 schema:genre research_article
44 schema:inLanguage en
45 schema:isAccessibleForFree false
46 schema:isPartOf N14966fcf52b144589e6ca24dcab0570c
47 N5d3cb0813b444789a476cf643b5047ff
48 sg:journal.1018957
49 schema:name Multi-ion pore behaviour in the CFTR chloride channel
50 schema:pagination 79-82
51 schema:productId N011926659b6f4dadbfbbafac33eb46f0
52 N1ceed328f091419597259b0cedda0409
53 N2527db03090e4b1cacc5acdf7cda8a4f
54 Ne1994589dc154cdca7550a8e2ea5e589
55 Nf770ffb51c09487f8b1a3714d0c9e998
56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029316472
57 https://doi.org/10.1038/366079a0
58 schema:sdDatePublished 2019-04-10T19:00
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher N5b8ef6c55d1846f2a7a085ef54dcfd75
61 schema:url http://www.nature.com/articles/366079a0
62 sgo:license sg:explorer/license/
63 sgo:sdDataset articles
64 rdf:type schema:ScholarlyArticle
65 N011926659b6f4dadbfbbafac33eb46f0 schema:name readcube_id
66 schema:value 8ba8d022320efb1c4490f876427b975122ee6a62507954ada25e58d53a4b6292
67 rdf:type schema:PropertyValue
68 N14966fcf52b144589e6ca24dcab0570c schema:volumeNumber 366
69 rdf:type schema:PublicationVolume
70 N17cacd268a95417380e303f8d6c7eb97 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Chlorides
72 rdf:type schema:DefinedTerm
73 N1cca4ddfe9c24f59a1cb869884818074 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Membrane Potentials
75 rdf:type schema:DefinedTerm
76 N1ceed328f091419597259b0cedda0409 schema:name pubmed_id
77 schema:value 7694154
78 rdf:type schema:PropertyValue
79 N2527db03090e4b1cacc5acdf7cda8a4f schema:name doi
80 schema:value 10.1038/366079a0
81 rdf:type schema:PropertyValue
82 N2a5403f0706244039958d50df151674a rdf:first sg:person.01243057047.05
83 rdf:rest N949f085e528a4f2b9ecc07d8d252b963
84 N3c17a6ce805f44189a3e8ea3820ceabf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Animals
86 rdf:type schema:DefinedTerm
87 N513510ed038c4223b0d18c81d54c8810 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Anions
89 rdf:type schema:DefinedTerm
90 N59928d3f9efe402db608480ded788b93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Membrane Proteins
92 rdf:type schema:DefinedTerm
93 N59a540ff4cb7495695e60681906c555f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Cricetinae
95 rdf:type schema:DefinedTerm
96 N5b8ef6c55d1846f2a7a085ef54dcfd75 schema:name Springer Nature - SN SciGraph project
97 rdf:type schema:Organization
98 N5cffb73bde284ab88c75599701c012a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Ion Channels
100 rdf:type schema:DefinedTerm
101 N5d3cb0813b444789a476cf643b5047ff schema:issueNumber 6450
102 rdf:type schema:PublicationIssue
103 N634e27e375b742aea0ba2744bb6f6132 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Molecular Sequence Data
105 rdf:type schema:DefinedTerm
106 N6a3aa1dc3d1b4a399af924ba376440cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Mutagenesis, Site-Directed
108 rdf:type schema:DefinedTerm
109 N6d64ceea5b634c5a93c1023d4155534c rdf:first sg:person.0732464671.04
110 rdf:rest rdf:nil
111 N8a37208f22774f98a8b73670398bdfce rdf:first sg:person.01310536143.87
112 rdf:rest N2a5403f0706244039958d50df151674a
113 N949f085e528a4f2b9ecc07d8d252b963 rdf:first sg:person.01110637425.01
114 rdf:rest N6d64ceea5b634c5a93c1023d4155534c
115 Na9742e2c24644bb3b5357851015db545 rdf:first sg:person.01361307674.99
116 rdf:rest N8a37208f22774f98a8b73670398bdfce
117 Naebb0412ce1e46f2b439c2d370483e61 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Cystic Fibrosis Transmembrane Conductance Regulator
119 rdf:type schema:DefinedTerm
120 Nb15ed794defc42178149f54ed6956ec5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name CHO Cells
122 rdf:type schema:DefinedTerm
123 Nb7d462416b6243829f2f8c5d24ab3faa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Chloride Channels
125 rdf:type schema:DefinedTerm
126 Nc0cc970b28ef4bdfaa911632c01838a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Cystic Fibrosis
128 rdf:type schema:DefinedTerm
129 Nd9a0f38f719541749f52ecb4437eb435 rdf:first sg:person.0617234553.55
130 rdf:rest Nf5aa05bc79134417943dda9a28485c81
131 Ndc4137379470443fb2eb17795ec443aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Arginine
133 rdf:type schema:DefinedTerm
134 Ne1994589dc154cdca7550a8e2ea5e589 schema:name nlm_unique_id
135 schema:value 0410462
136 rdf:type schema:PropertyValue
137 Ne583d11e5290469caa4ccd65600d467e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Amino Acid Sequence
139 rdf:type schema:DefinedTerm
140 Nf5aa05bc79134417943dda9a28485c81 rdf:first sg:person.01355172356.46
141 rdf:rest Na9742e2c24644bb3b5357851015db545
142 Nf770ffb51c09487f8b1a3714d0c9e998 schema:name dimensions_id
143 schema:value pub.1029316472
144 rdf:type schema:PropertyValue
145 Nf9062e766f9a43ddab51e194473a32ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Thiocyanates
147 rdf:type schema:DefinedTerm
148 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
149 schema:name Biological Sciences
150 rdf:type schema:DefinedTerm
151 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
152 schema:name Biochemistry and Cell Biology
153 rdf:type schema:DefinedTerm
154 sg:journal.1018957 schema:issn 0090-0028
155 1476-4687
156 schema:name Nature
157 rdf:type schema:Periodical
158 sg:person.01110637425.01 schema:familyName Riordan
159 schema:givenName J R
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110637425.01
161 rdf:type schema:Person
162 sg:person.01243057047.05 schema:familyName Tsui
163 schema:givenName L C
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243057047.05
165 rdf:type schema:Person
166 sg:person.01310536143.87 schema:familyName Chang
167 schema:givenName X B
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310536143.87
169 rdf:type schema:Person
170 sg:person.01355172356.46 schema:familyName Rommens
171 schema:givenName J M
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355172356.46
173 rdf:type schema:Person
174 sg:person.01361307674.99 schema:familyName Hou
175 schema:givenName Y X
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361307674.99
177 rdf:type schema:Person
178 sg:person.0617234553.55 schema:affiliation https://www.grid.ac/institutes/grid.14709.3b
179 schema:familyName Tabcharani
180 schema:givenName J A
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617234553.55
182 rdf:type schema:Person
183 sg:person.0732464671.04 schema:familyName Hanrahan
184 schema:givenName J W
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732464671.04
186 rdf:type schema:Person
187 sg:pub.10.1007/bf01871272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030522845
188 https://doi.org/10.1007/bf01871272
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/309453a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015077827
191 https://doi.org/10.1038/309453a0
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/347358a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035172743
194 https://doi.org/10.1038/347358a0
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/352628a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044633103
197 https://doi.org/10.1038/352628a0
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/362160a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026041580
200 https://doi.org/10.1038/362160a0
201 rdf:type schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1075993156 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1082464554 schema:CreativeWork
204 https://doi.org/10.1002/humu.1380010409 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037293842
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0014-5793(90)81257-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1006933539
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/0092-8674(90)90398-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007064578
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/0092-8674(91)90072-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040830484
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/0092-8674(91)90498-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1001366314
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/0092-8674(92)90155-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047418822
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0378-1119(89)90358-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021797759
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1085/jgp.61.6.687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032026837
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1093/hmg/2.1.51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059644060
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1093/nar/16.15.7351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002118380
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1113/jphysiol.1984.sp015352 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004807693
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1126/science.1712984 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062502499
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1152/ajpcell.1989.257.2.c240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078898779
229 rdf:type schema:CreativeWork
230 https://www.grid.ac/institutes/grid.14709.3b schema:alternateName McGill University
231 schema:name Department of Physiology, McGill University, Montréal, Québec, Canada.
232 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...