A tetrameric DNA structure with protonated cytosine-cytosine base pairs View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-06

AUTHORS

K Gehring, J L Leroy, M Guéron

ABSTRACT

Oligomers containing tracts of cytidine form hemiprotonated base pairs at acid pH and have been considered to be double-stranded. We have solved the structure of the DNA oligomer 5'-d(TCCCCC) at acid pH and find that it is a four-stranded complex in which two base-paired parallel-stranded duplexes are intimately associated, with their base pairs fully intercalated. The relative orientation of the duplexes is antiparallel, so that each base pair is face-to-face with its neighbours. The NMR spectrum displays only six spin systems, showing that the structure is highly symmetrical on the NMR timescale; the four strands are equivalent. A model derived by energy minimization and constrained molecular dynamics shows excellent compatibility with the observed nuclear Overhauser effects (NOEs) particularly for the very unusual inter-residue sugar-sugar NOEs H1'-H1', H1'-H2" and H1'-H4'. These NOEs are probably diagnostic for such tetrameric structures. More... »

PAGES

561-565

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/363561a0

DOI

http://dx.doi.org/10.1038/363561a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002150758

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8389423


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