Germ-line transmission and expression of a human-derived yeast artificial chromosome View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-03

AUTHORS

A Jakobovits, A L Moore, L L Green, G J Vergara, C E Maynard-Currie, H A Austin, S Klapholz

ABSTRACT

Introduction of DNA fragments, hundreds of kilobases in size, into mouse embryonic stem (ES) cells would greatly advance the ability to manipulate the mouse genome. Mice generated from such modified cells would permit investigation of the function and expression of very large or crudely mapped genes. Large DNA molecules cloned into yeast artificial chromosomes (YACs) are stable and genetically manipulable within yeast, suggesting yeast-cell fusion as an ideal method for transferring large DNA segments into mammalian cells. Introduction of YACs into different cell types by this technique has been reported; however, the incorporation of yeast DNA along with the YAC has raised doubts as to whether ES cells, modified in this way, would be able to recolonize the mouse germ line. Here we provide, to our knowledge, the first demonstration of germ-line transmission and expression of a large human DNA fragment, introduced into ES cells by fusion with yeast spheroplasts. Proper development was not impaired by the cointegration of a large portion of the yeast genome with the YAC. More... »

PAGES

255-258

Journal

TITLE

Nature

ISSUE

6417

VOLUME

362

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/362255a0

    DOI

    http://dx.doi.org/10.1038/362255a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1013946620

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8459850


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